pCMV-dRfxCas13d-Alkbh5-FL-Neo
$113.00
In stock
SKU
PL38827
100μg liquid plasmid(Instantly):$134
| name | pCMV-dRfxCas13d-Alkbh5-FL-Neo | another name | apCMV-dRfxCas13d-Alkbh5-FL-Neo |
| Insert Size (bp) | 4145bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCGAC GGATCGGGAG ATCTCCCGAT CCCCTATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGCCACCA TGATCGAAAA AAAAAAGTCC TTCGCCAAGG GCATGGGCGT GAAGTCCACA CTCGTGTCCG GCTCCAAAGT GTACATGACA ACCTTCGCCG AAGGCAGCGA CGCCAGGCTG GAAAAGATCG TGGAGGGCGA CAGCATCAGG AGCGTGAATG AGGGCGAGGC CTTCAGCGCT GAAATGGCCG ATAAAAACGC CGGCTATAAG ATCGGCAACG CCAAATTCAG CCATCCTAAG GGCTACGCCG TGGTGGCTAA CAACCCTCTG TATACAGGAC CCGTCCAGCA GGATATGCTC GGCCTGAAGG AAACTCTGGA AAAGAGGTAC TTCGGCGAGA GCGCTGATGG CAATGACAAT ATTTGTATCC AGGTGATCCA TAACATCCTG GACATTGAAA AAATCCTCGC CGAATACATT ACCAACGCCG CCTACGCCGT CAACAATATC TCCGGCCTGG ATAAGGACAT TATTGGATTC GGCAAGTTCT CCACAGTGTA TACCTACGAC GAATTCAAAG ACCCCGAGCA CCATAGGGCC GCTTTCAACA ATAACGATAA GCTCATCAAC GCCATCAAGG CCCAGTATGA CGAGTTCGAC AACTTCCTCG ATAACCCCAG ACTCGGCTAT TTCGGCCAGG CCTTTTTCAG CAAGGAGGGC AGAAATTACA TCATCAATTA CGGCAACGAA TGCTATGACA TTCTGGCCCT CCTGAGCGGA CTGGCGCACT GGGTGGTCGC TAACAACGAA GAAGAGTCCA GGATCTCCAG GACCTGGCTC TACAACCTCG ATAAGAACCT CGACAACGAA TACATCTCCA CCCTCAACTA CCTCTACGAC AGGATCACCA ATGAGCTGAC CAACTCCTTC TCCAAGAACT CCGCCGCCAA CGTGAACTAT ATTGCCGAAA CTCTGGGAAT CAACCCTGCC GAATTCGCCG AACAATATTT CAGATTCAGC ATTATGAAAG AGCAGAAAAA CCTCGGATTC AATATCACCA AGCTCAGGGA AGTGATGCTG GACAGGAAGG ATATGTCCGA GATCAGGAAA AATCATAAGG TGTTCGACTC CATCAGGACC AAGGTCTACA CCATGATGGA CTTTGTGATT TATAGGTATT ACATCGAAGA GGATGCCAAG GTGGCTGCCG CCAATAAGTC CCTCCCCGAT AATGAGAAGT CCCTGAGCGA GAAGGATATC TTTGTGATTA ACCTGAGGGG CTCCTTCAAC GACGACCAGA AGGATGCCCT CTACTACGAT GAAGCTAATA GAATTTGGAG AAAGCTCGAA AATATCATGC ACAACATCAA GGAATTTAGG GGAAACAAGA CAAGAGAGTA TAAGAAGAAG GACGCCCCTA GACTGCCCAG AATCCTGCCC GCTGGCCGTG ATGTTTCCGC CTTCAGCAAA CTCATGTATG CCCTGACCAT GTTCCTGGAT GGCAAGGAGA TCAACGACCT CCTGACCACC CTGATTAATA AATTCGATAA CATCCAGAGC TTCCTGAAGG TGATGCCTCT CATCGGAGTC AACGCTAAGT TCGTGGAGGA ATACGCCTTT TTCAAAGACT CCGCCAAGAT CGCCGATGAG CTGAGGCTGA TCAAGTCCTT CGCTAGAATG GGAGAACCTA TTGCCGATGC CAGGAGGGCC ATGTATATCG ACGCCATCCG TATTTTAGGA ACCAACCTGT CCTATGATGA GCTCAAGGCC CTCGCCGACA CCTTTTCCCT GGACGAGAAC GGAAACAAGC TCAAGAAAGG CAAGCACGGC ATGAGAAATT TCATTATTAA TAACGTGATC AGCAATAAAA GGTTCCACTA CCTGATCAGA TACGGTGATC CTGCCCACCT CCATGAGATC GCCAAAAACG AGGCCGTGGT GAAGTTCGTG CTCGGCAGGA TCGCTGACAT CCAGAAAAAA CAGGGCCAGA ACGGCAAGAA CCAGATCGAC AGGTACTACG AAACTTGTAT CGGAAAGGAT AAGGGCAAGA GCGTGAGCGA AAAGGTGGAC GCTCTCACAA AGATCATCAC CGGAATGAAC TACGACCAAT TCGACAAGAA AAGGAGCGTC ATTGAGGACA CCGGCAGGGA AAACGCCGAG AGGGAGAAGT TTAAAAAGAT CATCAGCCTG TACCTCACCG TGATCTACCA CATCCTCAAG AATATTGTCA ATATCAACGC CAGGTACGTC ATCGGATTCC ATTGCGTCGA GCGTGATGCT CAACTGTACA AGGAGAAAGG CTACGACATC AATCTCAAGA AACTGGAAGA GAAGGGATTC AGCTCCGTCA CCAAGCTCTG CGCTGGCATT GATGAAACTG CCCCCGATAA GAGAAAGGAC GTGGAAAAGG AGATGGCTGA AAGAGCCAAG GAGAGCATTG ACAGCCTCGA GAGCGCCAAC CCCAAGCTGT ATGCCAATTA CATCAAATAC AGCGACGAGA AGAAAGCCGA GGAGTTCACC AGGCAGATTA ACAGGGAGAA GGCCAAAACC GCCCTGAACG CCTACCTGAG GAACACCAAG TGGAATGTGA TCATCAGGGA GGACCTCCTG AGAATTGACA ACAAGACATG TACCCTGTTC GCAAACAAGG CCGTCGCCCT GGAAGTGGCC AGGTATGTCC ACGCCTATAT CAACGACATT GCCGAGGTCA ATTCCTACTT CCAACTGTAC CATTACATCA TGCAGAGAAT TATCATGAAT GAGAGGTACG AGAAAAGCAG CGGAAAGGTG TCCGAGTACT TCGACGCTGT GAATGACGAG AAGAAGTACA ACGATAGGCT CCTGAAACTG CTGTGTGTGC CTTTCGGCTA CTGTATCCCC AGGTTTAAGA ACCTGAGCAT CGAGGCCCTG TTCGATAGGA ACGAGGCCGC CAAGTTCGAC AAGGAGAAAA AGAAGGTGTC CGGCAATTCC ACCGGTCTGC CTCCACTTGA AAGACTGACA CTGGGATCCG GAGGAGGTGG AAGCGGTACC ATGGCGGCCG CCAGCGGCTA CACGGACCTG CGTGAGAAGC TCAAGTCCAT GACGTCCCGG GACAACTATA AGGCGGGCAG CCGGGAGGCC GCCGCCGCTG CCGCAGCCGC CGTAGCCGCC GCAGCCGCAG CCGCCGCTGC CGCCGAACCT TACCCTGTGT CCGGGGCCAA GCGCAAGTAT CAGGAGGACT CGGACCCCGA GCGCAGCGAC TATGAGGAGC AGCAGCTGCA GAAGGAGGAG GAGGCGCGCA AGGTGAAGAG CGGCATCCGC CAGATGCGCC TCTTCAGCCA GGACGAGTGC GCCAAGATCG AGGCCCGCAT TGACGAGGTG GTGTCCCGCG CTGAGAAGGG CCTGTACAAC GAGCACACGG TGGACCGGGC CCCACTGCGC AACAAGTACT TCTTCGGCGA AGGCTACACT TACGGCGCCC AGCTGCAGAA GCGCGGGCCC GGCCAGGAGC GCCTCTACCC GCCGGGCGAC GTGGACGAGA TCCCCGAGTG GGTGCACCAG CTGGTGATCC AAAAGCTGGT GGAGCACCGC GTCATCCCCG AGGGCTTCGT CAACAGCGCC GTCATCAACG ACTACCAGCC CGGCGGCTGC ATCGTGTCTC ACGTGGACCC CATCCACATC TTCGAGCGCC CCATCGTGTC CGTGTCCTTC TTTAGCGACT CTGCGCTGTG CTTCGGCTGC AAGTTCCAGT TCAAGCCTAT TCGGGTGTCG GAACCAGTGC TTTCCCTGCC GGTGCGCAGG GGAAGCGTGA CTGTGCTCAG TGGATATGCT GCTGATGAAA TCACTCACTG CATACGGCCT CAGGACATCA AGGAGCGCCG AGCAGTCATC ATCCTCAGGA AGACAAGATT AGATGCACCC CGGTTGGAAA CAAAGTCCCT GAGCAGCTCC GTGTTACCAC CCAGCTATGC TTCAGATCGC CTGTCAGGAA ACAACAGGGA CCCTGCTCTG AAACCCAAGC GGTCCCACCG CAAGGCAGAC CCTGATGCTG CCCACAGGCC ACGGATCCTG GAGATGGACA AGGAAGAGAA CCGGCGCTCG GTGCTGCTGC CCACACACCG GCGGAGGGGT AGCTTCAGCT CTGAGAACTA CTGGCGCAAG TCATACGAGT CCTCAGAGGA CTGCTCTGAG GCAGCAGGCA GCCCTGCCCG AAAGGTGAAG ATGCGGCGGC ACTGAGAATT CGCGGCCGCT CGAGTCTAGA GGGCCCGTTT AAACCCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCAGCT GGGGCTCTAG GGGGTATCCC CACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT AATTCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCA
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