pCMV-CUL9(human)-3×HA-Neo
$157.00
In stock
SKU
PL38815
NM_015089.4;H7AP1; PARC
100μg liquid plasmid(Instantly):$178
| name | pCMV-CUL9(human)-3×HA-Neo | another name | apCMV-CUL9(human)-3×HA-Neo |
| Insert Size (bp) | 7551bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCATGGT GGGGGAACGG CATGCTGGGG ACCTCATGGT GCCCTTAGGG CCTCGGCTGC AGGCATATCC TGAAGAACTC ATTCGACAGA GGCCTGGGCA TGACGGGCAT CCTGAATACC TGATCCGATG GAGTGTCCTG AAGTGTGGGG AAGTGGGCAA AGTGGGTGTG GAAGAAGGCA AAGCAGAGCA CATCCTCATG TGGCTGTCGG CTCCTGAGGT CTACGCCAAC TGCCCTGGGC TGTTAGGTGA GCGGGCACTA TCTAAGGGAC TTCAGCACGA ACCAGCTGGG GTTTCAGGAA GCTTTCCTCG AGATCCAGGA GGCCTGGATG AAGTGGCAAT GGGAGAGATG GAGGCTGATG TTCAGGCGCT GGTACGCAGG GCGGCCAGGC AGCTGGCAGA AAGTGGGACC CCAAGCCTCA CGGCCGCTGT GCTTCACACC ATCCACGTGC TCAGTGCCTA CGCCAGCATC GGGCCCCTCA CTGGTGTCTT CAGGGAGACA GGAGCCCTGG ACCTGCTCAT GCACATGTTA TGCAATCCTG AGCCTCAGAT CCGCCGGAGT GCAGGCAAAA TGCTGCAGGC TCTGGCAGCC CACGATGCTG GGAGTCGGGC TCACGTCCTT CTATCACTGA GCCAGCAAGA TGGCATCGAG CAGCACATGG ATTTTGACAG TCGCTATACA TTGCTGGAGC TGTTTGCAGA AACCACATCC TCTGAAGAAC ACTGCATGGC CTTTGAGGGC ATTCATCTGC CTCAGATCCC AGGAAAGCTG CTTTTCTCCT TGGTGAAGCG CTACCTTTGT GTCACGTCCC TCCTGGATCA GCTGAATAGC AGTCCAGAGC TGGGAGCTGG AGACCAAAGC TCCCCATGTG CCACAAGAGA GAAAAGCCGG GGACAGCGGG AACTGGAGTT CAGCATGGCT GTGGGCAACC TCATCTCTGA GCTTGTGCGG AGCATGGGCT GGGCCCGGAA CCTCAGCGAA CAGGGCATGT CACCTCCCCG GCCAACCCGG TCCATCTTTC AGCCCTACAT TTCAGGCCCC AGCCTTTTAC TCCCCACCAT TGTCACCACC CCCAGAAGAC AAGGGTGGGT CTTCCGCCAG CGCTCTGAAT TCTCCAGCCG TAGTGGCTAT GGAGAATATG TGCAGCAGAC ACTGCAGCCA GGGATGCGAG TGCGGATGCT GGATGATTAT GAGGAGATCA GTGCTGGGGA CGAGGGCGAG TTCCGGCAGA GCAACAACGG CATTCCCCCT GTGCAGGTTT TCTGGCAGTC GACAGGCCGC ACTTACTGGG TGCACTGGCA CATGCTGGAG ATCCTGGGCC CTGAGGAAGC CACTGAGGAT AAGGCTTCAG CAGCTGTGGA GAAGGGGGCA GGGGCTACTG TGTTGGGCAC AGCATTTCCC TCCTGGGACT GGAATCCTAT GGATGGGCTG TACCCTTTGC CGTACCTCCA GCCCGAACCT CAGAAGAATG AGAGAGTGGG ATATCTGACC CAGGCTGAAT GGTGGGAGCT GCTTTTCTTT ATCAAAAAGT TGGACTTGTG TGAGCAGCAG CCAATTTTCC AGAATCTTTG GAAGAACCTG GATGAGACCC TGGGTGAAAA GGCCCTAGGT GAGATCTCTG TGTCCGTGGA AATGGCCGAG AGTCTGCTGC AGGTTCTCAG TAGTCGATTT GAGGGCAGCA CTCTCAATGA CCTGCTCAAC TCCCAGATCT ACACCAAGTA TGGGCTGCTG TCTAATGAAC CAAGCAGCTC GTCTACTTCA CGAAATCACT CCTGTACCCC AGATCCAGAA GAGGAGTCCA AGTCGGAGGC CAGCTTCTCA GAGGAAGAGA CTGAGTCCCT CAAAGCAAAG GCCGAGGCCC CTAAGACAGA GGCCGAGCCC ACCAAGACAA GGACCGAGAC CCCCATGGCA CAGAGTGATT CTCAGCTGTT TAACCAGCTT CTGGTGACTG AGGGGATGAC CCTGCCCACT GAGATGAAGG AGGCAGCCAG TGAAATGGCC AGAGCCTTGC GGGGTCCCGG TCCTCGCAGC TCCCTGGATC AGCATGTGGC AGCGGTCGTG GCCACTGTGC AGATATCCAG CTTGGACACA AACCTGCAGC TTTCAGGGCT CTCTGCCCTC TCTCAGGCTG TGGAGGAGGT CACTGAGCGG GACCACCCTC TGGTCCGTCC TGACAGATCG CTGAGAGAGA AGCTAGTGAA GATGCTGGTG GAGCTGCTGA CCAACCAGGT GGGAGAGAAG ATGGTGGTCG TGCAGGCCCT GCGCCTCCTT TACCTGCTCA TGACCAAGCA CGAGTGGCGG CCGCTCTTTG CCAGGGAGGG TGGCATCTAT GCTGTGCTGG TCTGCATGCA AGAATATAAG ACTTCTGTCT TGGTGCAGCA GGCTGGGCTG GCGGCACTGA AGATGCTGGC CGTCGCCAGC TCCTCGGAGA TCCCCACTTT TGTTACTGGC CGAGATTCTA TCCACTCTTT GTTTGATGCT CAGATGACCA GAGAGATCTT CGCCAGCATC GACTCAGCCA CACGCCCGGG CTCTGAGAGC CTGCTCCTCA CTGTCCCTGC AGCCGTGATC CTGATGCTGA ATACTGAGGG GTGCTCTTCT GCAGCGAGAA ATGGCTTACT CCTGCTCAAC CTACTTTTGT GCAACCACCA CACTCTGGGA GACCAGATTA TAACCCAAGA GCTGAGAGAC ACGTTGTTTA GGCACTCAGG GATAGCACCA AGAACAGAAC CTATGCCTAC CACACGCACC ATCCTCATGA TGCTTCTCAA TCGCTACTCA GAGCCGCCGG GCAGCCCTGA GCGTGCAGCA CTAGAGACCC CCATCATCCA GGGTCAGGAT GGGTCCCCTG AGCTACTGAT TCGATCCCTG GTTGGGGGCC CATCTGCAGA ACTACTCCTG GACTTGGAGC GTGTGCTGTG CCGTGAGGGC AGCCCCGGAG GTGCCGTGAG GCCCCTCCTC AAGCGCCTCC AGCAGGAGAC CCAGCCTTTC CTCCTGTTGC TGCGGACTCT GGATGCTCCG GGGCCCAACA AGACTCTGCT GCTGTCTGTG CTGAGGGTCA TAACCCGACT GCTGGATTTC CCTGAGGCAA TGGTCCTCCC CTGGCACGAG GTCTTGGAGC CCTGCCTCAA CTGCCTGAGT GGCCCTAGCA GTGACTCCGA GATTGTTCAG GAGCTGACCT GCTTCCTACA TCGCCTGGCC TCGATGCATA AGGACTATGC TGTGGTGCTC TGCTGCCTGG GAGCAAAAGA GATCCTCTCC AAAGTCCTGG ACAAGCACTC AGCTCAGCTG CTGCTGGGCT GTGAGCTTCG GGACCTGGTG ACAGAGTGTG AGAAGTACGC ACAGCTCTAT AGCAACCTCA CCTCCAGCAT CCTGGCCGGC TGCATTCAGA TGGTGCTGGG CCAGATCGAA GACCACAGAC GAACCCACCA ACCCATCAAT ATCCCCTTCT TTGATGTGTT CCTCAGGCAT CTCTGCCAGG GCTCCAGTGT GGAAGTGAAG GAGGACAAGT GCTGGGAGAA GGTGGAGGTG TCCTCCAACC CGCACCGAGC CAGCAAGCTG ACGGACCACA ACCCCAAGAC CTACTGGGAG TCCAACGGCA GCACCGGCTC CCACTACATC ACCCTGCACA TGCACCGTGG TGTTCTTGTT AGGCAGCTCA CTTTGCTGGT GGCCAGTGAG GACTCAAGCT ACATGCCAGC CAGGGTGGTG GTGTTTGGGG GTGACAGCAC CAGCTGCATC GGCACTGAGC TCAACACGGT GAATGTGATG CCCTCTGCCA GCCGGGTGAT CCTCTTGGAG AACCTGAACC GCTTCTGGCC CATCATCCAG ATCCGCATAA AGCGCTGCCA GCAGGGCGGC ATTGACACCC GGGTTCGGGG TGTGGAGGTC CTGGGCCCTA AGCCCACATT CTGGCCACTG TTCCGGGAGC AGCTGTGTCG CCGAACATGT CTCTTCTACA CAATTCGGGC ACAAGCCTGG AGCCGGGACA TAGCAGAGGA CCACCGGCGC CTCCTCCAGC TCTGTCCCAG ACTGAACAGG GTTTTGCGCC ACGAGCAGAA TTTTGCTGAC CGCTTCCTCC CTGATGATGA GGCCGCCCAG GCACTGGGCA AGACCTGCTG GGAGGCCCTG GTCAGCCCCC TGGTGCAGAA CATCACCTCT CCCGATGCGG AAGGCGTGAG TGCCCTGGGA TGGCTGCTGG ATCAGTACTT AGAACAGAGA GAGACCTCTC GGAACCCCTT GAGTCGAGCA GCGTCCTTTG CTTCTCGAGT TCGTCGCCTT TGCCACTTGC TGGTGCATGT GGAACCTCCT CCTGGGCCTT CTCCTGAGCC ATCCACTCGG CCCTTCAGCA AGAACAGCAA GGGTCGGGAC CGGAGCCCGG CGCCTTCGCC AGTGCTTCCA AGCAGCAGCC TGAGGAACAT AACCCAGTGC TGGCTGAGCG TGGTGCAGGA GCAGGTCAGC AGATTCCTGG CTGCAGCTTG GAGGGCCCCA GACTTTGTGC CTCGTTACTG TAAACTCTAT GAGCACTTGC AGAGAGCAGG CTCCGAGCTG TTTGGGCCTC GGGCAGCCTT CATGCTGGCT CTGCGCAGTG GCTTCTCTGG CGCCTTGCTG CAGCAGTCCT TCCTCACTGC TGCTCACATG AGTGAGCAGT TTGCCAGGTA CATTGACCAA CAGATCCAGG GTGGCCTGAT TGGTGGAGCC CCTGGAGTGG AAATGCTGGG GCAGCTTCAG CGGCACCTGG AACCCATTAT GGTCCTTTCT GGTCTGGAAC TGGCCACAAC TTTTGAGCAC TTCTATCAGC ATTATATGGC GGACCGTCTC CTGAGCTTTG GTTCGAGCTG GCTGGAGGGG GCTGTGCTAG AGCAGATTGG CCTCTGTTTT CCCAACCGCC TCCCACAGCT GATGCTGCAG AGCCTGAGCA CCTCTGAGGA GCTGCAGCGC CAGTTCCACC TCTTCCAGCT CCAGCGGCTC GACAAGTTGT TCTTGGAGCA GGAAGATGAG GAGGAAAAGA GACTAGAGGA AGAGGAGGAG GAAGAGGAGG AAGAGGAAGC TGAGAAAGAA TTATTTATCG AAGATCCAAG TCCAGCCATT TCTATACTGG TCCTGTCACC ACGCTGCTGG CCCGTCTCCC CACTCTGCTA CCTGTACCAT CCCAGAAAGT GCCTTCCCAC AGAATTCTGT GATGCCCTTG ACCGTTTCTC CAGTTTCTAC AGCCAGAGTC AGAACCATCC AGTCCTGGAC ATGGGACCAC ATCGGCGACT GCAGTGGACG TGGCTGGGCC GGGCTGAGCT GCAGTTTGGG AAGCAGATAC TGCATGTGTC CACCGTGCAG ATGTGGCTGC TGCTGAAATT CAATCAGACA GAGGAGGTGT CAGTAGAGAC CTTGCTGAAG GATTCTGACC TCTCCCCAGA GCTGCTGCTC CAGGCACTCG TGCCCCTCAC CTCAGGGAAT GGCCCTTTGA CCCTGCATGA GGGCCAGGAC TTTCCACACG GGGGTGTGCT GCGGCTTCAT GAGCCTGGGC CCCAGCGCAG TGGGGAGGCC CTGTGGCTGA TACCTCCCCA GGCATACCTG AACGTAGAGA AGGATGAAGG CCGAACCCTG GAACAGAAGA GGAATCTCTT GAGCTGTCTT CTTGTTCGTA TTCTCAAAGC CCATGGGGAA AAGGGCCTCC ACATTGATCA GCTGGTTTGT CTGGTGCTGG AGGCCTGGCA GAAGGGTCCA AATCCTCCTG GAACCCTGGG CCACACTGTT GCTGGGGGTG TGGCCTGTAC CAGTACAGAT GTCCTCTCTT GCATCCTGCA CCTCTTAGGC CAGGGCTACG TGAAACGGCG TGATGACCGG CCCCAGATCC TGATGTATGC CGCTCCAGAG CCCATGGGGC CCTGCCGGGG TCAGGCAGAT GTCCCTTTCT GTGGCAGCCA GAGCGAAACC TCCAAGCCCA GCCCAGAAGC TGTGGCTACC CTGGCATCTC TACAGCTGCC TGCAGGCCGC ACCATGAGCC CCCAGGAAGT AGAAGGGTTG ATGAAGCAGA CGGTGCGTCA GGTGCAGGAG ACGCTGAACT TAGAGCCAGA TGTCGCTCAG CACCTTTTGG CTCATTCCCA CTGGGGCGCT GAACAGCTGC TGCAGAGCTA CAGTGAGGAC CCTGAGCCAC TGCTGCTGGC AGCTGGGCTG TGCGTACACC AGGCTCAGGC TGTACCCGTA CGGCCTGACC ACTGCCCCGT CTGTGTGAGC CCCCTGGGGT GTGACGACGA CCTGCCCTCT CTCTGCTGCA TGCACTATTG CTGTAAGTCT TGCTGGAATG AGTACCTGAC AACTCGGATC GAGCAGAACC TTGTTTTGAA TTGCACCTGC CCCATTGCCG ACTGCCCCGC CCAGCCCACC GGAGCCTTCA TTCGTGCCAT CGTCTCCTCG CCAGAGGTCA TCTCCAAGTA TGAGAAGGCG CTCCTGCGTG GCTATGTGGA GAGCTGCTCC AACCTGACCT GGTGCACCAA CCCCCAGGGC TGCGACCGCA TCCTGTGCCG CCAGGGCCTG GGCTGTGGGA CCACCTGCTC CAAGTGTGGC TGGGCCTCTT GCTTCAACTG TAGCTTCCCT GAGGCACACT ACCCTGCTAG CTGTGGCCAT ATGTCTCAGT GGGTCGACGA CGGTGGCTAC TATGACGGCA TGAGCGTGGA GGCGCAGAGC AAGCACCTGG CCAAGCTCAT CTCCAAGCGC TGTCCCAGCT GTCAGGCTCC CATCGAGAAG AACGAGGGGT GCCTGCACAT GACCTGTGCC AAATGTAACC ATGGATTCTG CTGGCGCTGC CTCAAGTCCT GGAAGCCAAA TCACAAAGAC TATTACAACT GCTCTGCCAT GGTAAGCAAG GCAGCTCGCC AGGAGAAGCG GTTTCAGGAC TATAATGAGA GGTGCACTTT CCATCACCAG GCGCGGGAGT TTGCTGTGAA CTTGCGGAAC CGGGTGTCTG CCATCCATGA AGTGCCCCCG CCCAGATCCT TCACCTTCCT CAATGATGCC TGCCAGGGAC TGGAGCAGGC TCGGAAGGTG CTGGCCTACG CCTGCGTGTA CAGCTTCTAC AGCCAGGACG CAGAGTACAT GGATGTGGTG GAGCAGCAGA CAGAGAACCT GGAGCTGCAC ACCAATGCCC TGCAGATCCT CCTGGAGGAA ACCCTGCTGC GGTGCAGAGA CCTGGCCTCC TCCCTGCGCC TCCTGCGGGC CGACTGCCTC AGCACGGGCA TGGAGCTGCT CCGGCGGATC CAGGAGAGGC TGCTTGCCAT CCTGCAGCAT TCTGCCCAGG ATTTCCGGGT TGGTCTTCAG AGTCCATCAG TAGAGGCCTG GGAGGCAAAA GGACCCAACA TGCCTGGCAG TCAGCCCCAG GCCTCCTCAG GGCCAGAGGC AGAAGAGGAG GAGGAAGACG ATGAGGATGA TGTGCCCGAG TGGCAGCAGG ATGAGTTTGA TGAGGAGCTG GACAATGACA GCTTCTCCTA CGATGAGTCT GAGAACCTGG ACCAAGAGAC TTTCTTCTTT GGTGATGAGG AAGAGGATGA AGATGAGGCC TATGACCTCG AGTACCCATA CGACGTACCT GATTATGCAA CTTATCCTTA TGATGTACCT GATTATGCTA GCTATCCTTA TGATGTCCCA GACTACGCAT AGTGAGGGCC CGATATCTCG ACAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAGCT GACGTCCTTT CCATGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG CCTGGAAACG GGGGAGGCTA ACTGAAACAC GGAAGGAGAC AATACCGGAA GGAACCCGCG CTATGACGGC AATAAAAAGA CAGAATAAAA CGCACGGGTG TTGGGTCGTT TGTTCATAAA CGCGGGGTTC GGTCCCAGGG CTGGCACTCT GTCGATACCC CACCGAGACC CCATTGGGGC CAATACGCCC GCGTTTCTTC CTTTTCCCCA CCCCACCCCC CAAGTTCGGG TGAAGGCCCA GGGCTCGCAG CCAACGTCGG GGCGGCAGGC CCTGCCATAG CAGATCTGCG CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGCATCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGGGG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC AATGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCGACGGATC GGGAGATCTC CCGATCCCCT ATGGTCGACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATCTGCTCCC TGCTTGTGTG TTGGAGGTCG CTGAGTAGTG CGCGAGCAAA ATTTAAGCTA CAACAAGGCA AGGCTTGACC GACAATTGCA TGAAGAATCT GCTTAGG
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