NM_000138.5;ACMICD; ECTOL1; FBN; GPHYSD2; MASS; MFLS;MFS1; OCTD; SGS; SSKS; WMS; WMS2
100μg liquid plasmid(Instantly):$192
| name | pLV3-CMV-FBN1(human)-3×HA-CopGFP-Puro | another name | apLV3-CMV-FBN1(human)-3×HA-CopGFP-Puro |
| Insert Size (bp) | 8613bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT TATCGATACT AGTATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTTT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGATTC TAGAGCTAGC CTGATACGAA CTCGGAATTC GCCACCATGC GTCGAGGGCG TCTGCTGGAG ATCGCCCTGG GATTTACCGT GCTTTTAGCG TCCTACACGA GCCATGGGGC GGACGCCAAT TTGGAGGCTG GGAACGTGAA GGAAACCAGA GCCAGTCGGG CCAAGAGAAG AGGCGGTGGA GGACACGACG CGCTTAAAGG ACCCAATGTC TGTGGATCAC GTTATAATGC TTACTGTTGC CCTGGATGGA AAACCTTACC TGGCGGAAAT CAGTGTATTG TCCCCATTTG CCGGCATTCC TGTGGGGATG GATTTTGTTC GAGGCCAAAT ATGTGCACTT GCCCATCTGG TCAGATAGCT CCTTCCTGTG GCTCCAGATC CATACAACAC TGCAATATTC GCTGTATGAA TGGAGGTAGC TGCAGTGACG ATCACTGTCT ATGCCAGAAA GGATACATAG GGACTCACTG TGGACAACCT GTTTGTGAAA GTGGCTGTCT CAATGGAGGA AGGTGTGTGG CCCCAAATCG ATGTGCATGC ACTTACGGAT TTACTGGACC CCAGTGTGAA AGAGATTACA GGACAGGCCC ATGTTTTACT GTGATCAGCA ACCAGATGTG CCAGGGACAA CTCAGCGGGA TTGTCTGCAC AAAAACGCTC TGCTGTGCCA CAGTCGGCCG AGCCTGGGGC CACCCCTGTG AGATGTGTCC TGCCCAGCCT CACCCCTGCC GCCGTGGCTT CATTCCAAAT ATCCGCACGG GAGCTTGTCA AGATGTGGAT GAATGCCAGG CCATCCCCGG GCTCTGTCAG GGAGGAAATT GCATTAATAC TGTTGGGTCT TTTGAGTGCA AATGCCCTGC TGGACACAAA CTTAATGAAG TGTCACAAAA ATGTGAAGAT ATTGATGAAT GCAGCACCAT TCCTGGAATC TGTGAAGGGG GTGAATGTAC AAACACAGTC AGCAGTTACT TTTGCAAATG TCCCCCTGGT TTTTACACCT CTCCAGATGG TACCAGATGC ATAGATGTTC GCCCAGGATA CTGTTACACA GCTCTGACAA ACGGGCGCTG CTCTAACCAG CTGCCACAGT CCATAACCAA AATGCAGTGC TGCTGTGATG CCGGCCGATG CTGGTCTCCA GGGGTCACTG TCGCCCCTGA GATGTGTCCC ATCAGAGCAA CCGAGGATTT CAACAAGCTG TGCTCTGTTC CTATGGTAAT TCCTGGGAGA CCAGAATATC CTCCCCCACC CCTTGGCCCC ATTCCTCCAG TTCTCCCTGT TCCTCCTGGC TTTCCTCCTG GACCTCAAAT TCCGGTCCCT CGACCACCAG TGGAATATCT GTATCCATCT CGGGAGCCAC CAAGGGTGCT GCCAGTAAAC GTTACTGATT ACTGCCAGTT GGTCCGCTAT CTCTGTCAAA ATGGACGCTG CATTCCAACT CCTGGGAGTT ACCGGTGTGA GTGCAACAAA GGGTTCCAGC TGGACCTCCG TGGGGAGTGT ATTGATGTTG ATGAATGTGA GAAAAACCCC TGTGCTGGTG GTGAGTGTAT TAACAACCAG GGTTCGTACA CCTGTCAGTG CCGAGCTGGA TATCAGAGCA CACTCACGCG GACAGAATGC CGAGACATTG ATGAGTGTTT ACAGAATGGC CGGATCTGCA ATAATGGACG CTGCATCAAC ACAGATGGCA GTTTTCATTG CGTGTGTAAT GCGGGCTTTC ATGTTACACG AGATGGGAAG AACTGTGAAG ATATGGATGA ATGCAGCATA AGGAACATGT GCCTTAATGG AATGTGTATC AATGAAGATG GCAGTTTTAA ATGTATTTGC AAACCTGGAT TCCAGCTGGC ATCAGATGGA CGTTATTGCA AAGACATTAA CGAGTGTGAA ACCCCTGGGA TCTGCATGAA TGGGCGTTGC GTCAACACTG ATGGCTCCTA CAGATGTGAA TGCTTCCCTG GACTGGCTGT GGGTCTGGAT GGCCGTGTGT GTGTTGACAC ACACATGCGG AGCACATGCT ATGGTGGATA CAAGAGAGGC CAGTGTATCA AACCTTTGTT TGGTGCTGTC ACTAAATCTG AATGCTGTTG CGCCAGCACT GAGTATGCAT TTGGGGAACC TTGCCAGCCG TGTCCTGCAC AGAATTCAGC GGAATATCAG GCACTCTGCA GCAGTGGGCC AGGAATGACG TCAGCAGGCA GTGATATAAA TGAATGTGCA CTAGATCCTG ATATTTGCCC AAATGGAATC TGTGAAAACC TTCGTGGGAC CTATAAATGT ATATGCAATT CAGGATATGA AGTGGATTCA ACTGGGAAAA ACTGCGTTGA TATTAATGAA TGTGTACTGA ACAGTCTCCT TTGTGACAAT GGACAATGTA GAAATACTCC TGGAAGTTTT GTCTGTACCT GCCCCAAGGG ATTTATCTAC AAACCTGATC TAAAAACATG TGAAGACATT GATGAATGCG AATCAAGTCC TTGCATTAAT GGAGTCTGCA AGAACAGCCC AGGCTCTTTT ATTTGTGAAT GTTCTTCTGA AAGTACTTTG GATCCAACAA AAACCATCTG CATAGAAACC ATCAAGGGCA CTTGCTGGCA GACTGTCATT GATGGGCGAT GTGAGATCAA CATCAATGGA GCCACCTTAA AGTCCCAGTG CTGCTCCTCC CTCGGTGCTG CGTGGGGAAG CCCGTGCACC CTATGCCAAG TTGATCCCAT ATGTGGTAAA GGGTACTCAA GAATTAAAGG AACACAATGT GAAGATATAG ATGAATGTGA AGTGTTCCCA GGAGTGTGTA AAAATGGCCT GTGTGTTAAC ACTAGGGGGT CATTCAAGTG TCAGTGTCCC AGTGGAATGA CTTTGGATGC CACAGGAAGG ATCTGTCTTG ATATCCGCCT GGAAACCTGC TTCCTGAGGT ACGAGGACGA GGAGTGCACC CTGCCTATTG CTGGCCGCCA CCGCATGGAC GCCTGCTGCT GCTCCGTCGG GGCAGCCTGG GGTACTGAGG AATGCGAGGA GTGTCCCATG AGAAATACTC CTGAGTACGA GGAGCTGTGT CCGAGAGGAC CCGGATTTGC CACAAAAGAA ATTACAAATG GAAAGCCTTT CTTCAAAGAT ATCAATGAGT GCAAGATGAT ACCCAGCCTC TGCACCCACG GCAAGTGCAG AAACACCATT GGCAGCTTTA AGTGCAGGTG TGACAGCGGC TTTGCTCTTG ATTCTGAAGA AAGGAACTGC ACAGACATTG ACGAATGCCG CATATCTCCT GACCTCTGTG GCAGAGGCCA GTGTGTGAAC ACCCCTGGGG ACTTTGAATG CAAGTGTGAC GAAGGCTATG AAAGTGGATT CATGATGATG AAGAACTGCA TGGATATTGA TGAGTGTCAG AGAGATCCTC TCCTATGCCG AGGTGGTGTT TGCCATAACA CAGAGGGAAG TTACCGCTGT GAATGCCCGC CTGGCCATCA GCTGTCCCCC AACATCTCCG CGTGTATCGA CATCAATGAA TGTGAGCTGA GTGCACACCT GTGCCCCAAT GGCCGTTGCG TGAACCTCAT AGGGAAGTAT CAGTGTGCCT GCAACCCTGG CTACCATTCA ACTCCCGATA GGCTATTTTG TGTTGACATT GATGAATGCA GCATAATGAA TGGTGGTTGT GAAACCTTCT GCACAAACTC TGAAGGCAGC TATGAATGTA GCTGTCAGCC GGGATTTGCA CTAATGCCTG ACCAGAGATC ATGCACCGAC ATCGATGAGT GTGAAGATAA TCCCAATATC TGTGATGGTG GTCAGTGCAC AAATATCCCT GGAGAGTACA GGTGCTTGTG TTATGATGGA TTCATGGCAT CTGAAGACAT GAAGACTTGT GTAGATGTCA ATGAGTGTGA CCTGAATCCA AATATCTGCC TAAGTGGGAC CTGTGAAAAC ACGAAAGGCT CATTTATCTG CCACTGTGAT ATGGGCTACT CCGGCAAAAA AGGAAAAACT GGCTGTACAG ACATCAATGA ATGTGAAATT GGAGCACACA ACTGTGGCAA ACATGCTGTA TGTACCAATA CAGCAGGAAG CTTCAAATGT AGCTGCAGTC CCGGGTGGAT TGGAGATGGC ATTAAGTGCA CTGATCTGGA CGAATGTTCC AATGGAACCC ATATGTGCAG CCAGCATGCA GACTGCAAGA ATACCATGGG ATCTTACCGC TGTCTGTGCA AGGAAGGATA CACAGGTGAT GGCTTCACTT GTACAGACCT TGATGAGTGC TCTGAGAACC TGAATCTCTG TGGCAATGGC CAGTGCCTCA ATGCACCAGG AGGATACCGC TGTGAATGCG ACATGGGCTT CGTGCCCAGT GCTGACGGGA AAGCCTGTGA AGATATTGAT GAGTGCTCCC TTCCGAACAT CTGTGTCTTT GGAACTTGCC ACAACCTCCC TGGCCTGTTC CGCTGTGAGT GTGAGATAGG CTACGAACTG GACAGAAGCG GCGGGAACTG CACAGATGTG AATGAATGCC TGGATCCAAC CACGTGCATC AGTGGGAACT GTGTCAACAC TCCAGGCAGC TATATCTGTG ACTGCCCACC TGATTTTGAA CTGAACCCAA CTCGAGTTGG CTGTGTTGAT ACCCGCTCTG GAAATTGCTA TTTGGATATT CGACCTCGAG GAGACAATGG AGATACAGCC TGCAGCAATG AAATTGGAGT TGGTGTTTCC AAAGCTTCCT GCTGCTGTTC TCTGGGTAAA GCCTGGGGTA CTCCTTGTGA GATGTGTCCT GCTGTGAACA CATCCGAGTA CAAAATTCTT TGTCCTGGAG GGGAAGGTTT CCGACCAAAT CCTATCACCG TTATATTGGA AGATATTGAT GAGTGCCAGG AGCTACCAGG GCTGTGCCAA GGAGGAAAAT GTATCAACAC CTTTGGGAGT TTCCAGTGCC GCTGTCCAAC CGGCTACTAC CTGAATGAAG ATACACGAGT GTGTGATGAT GTGAATGAAT GTGAGACTCC TGGAATCTGT GGTCCAGGGA CATGTTACAA CACCGTTGGC AACTACACCT GTATCTGTCC TCCAGACTAC ATGCAAGTGA ATGGGGGAAA TAATTGCATG GATATGAGAA GAAGTTTGTG CTACAGAAAC TACTATGCTG ACAACCAGAC CTGTGATGGA GAATTGTTAT TCAACATGAC CAAGAAGATG TGCTGCTGTT CCTACAACAT TGGCCGGGCG TGGAACAAGC CCTGTGAACA GTGTCCCATC CCAAGTACAG ATGAGTTTGC TACACTCTGT GGAAGTCAAA GGCCAGGCTT TGTCATCGAC ATTTATACCG GTTTACCCGT TGATATTGAT GAGTGCCGGG AGATCCCAGG GGTCTGTGAA AATGGAGTGT GTATCAACAT GGTTGGCAGC TTCCGATGTG AATGTCCAGT GGGATTCTTC TATAATGACA AGTTGTTGGT TTGTGAAGAT ATTGACGAGT GTCAGAACGG CCCAGTGTGC CAGCGCAACG CCGAATGCAT CAACACTGCA GGCAGCTACC GCTGTGACTG TAAGCCCGGC TACCGCTTCA CCTCCACAGG ACAGTGCAAT GATCGTAATG AATGTCAAGA AATCCCCAAT ATATGCAGTC ATGGGCAGTG CATTGACACA GTTGGAAGCT TTTATTGCCT TTGCCACACT GGTTTTAAAA CAAATGATGA CCAAACCATG TGCTTGGACA TAAATGAATG TGAAAGAGAT GCCTGTGGGA ATGGAACTTG CCGGAACACA ATTGGTTCCT TCAACTGCCG CTGCAATCAT GGTTTCATCC TTTCTCACAA CAATGACTGT ATAGATGTTG ATGAATGTGC AAGTGGAAAT GGGAATCTTT GCAGAAATGG CCAATGCATT AATACAGTGG GGTCTTTCCA GTGCCAGTGC AATGAAGGCT ATGAGGTGGC TCCAGATGGG AGGACCTGTG TGGATATCAA TGAATGTCTT CTAGAACCCA GAAAATGTGC ACCAGGTACC TGTCAAAACT TGGATGGGTC CTACAGATGC ATTTGCCCAC CTGGATACAG TCTTCAAAAT GAGAAGTGTG AAGATATTGA TGAGTGTGTC GAAGAGCCAG AAATTTGTGC CCTGGGCACA TGCAGTAACA CTGAAGGCAG CTTCAAATGT CTGTGTCCAG AAGGGTTTTC CTTGTCCTCC AGTGGAAGAA GGTGCCAAGA TTTGCGAATG AGCTACTGTT ATGCGAAGTT TGAAGGAGGA AAGTGTTCAT CACCCAAATC CAGAAATCAC TCCAAGCAGG AATGCTGCTG TGCCTTGAAG GGAGAAGGCT GGGGAGACCC CTGCGAGCTC TGCCCCACGG AACCTGATGA GGCCTTCCGC CAGATATGTC CTTATGGAAG TGGGATCATC GTGGGACCTG ATGATTCAGC AGTTGATATG GACGAATGCA AAGAACCCGA TGTCTGTAAA CATGGACAGT GCATCAATAC AGATGGTTCC TATCGCTGCG AGTGTCCCTT TGGTTATATT CTAGCAGGGA ATGAATGTGT AGATACTGAT GAATGTTCTG TTGGCAATCC TTGTGGAAAT GGAACCTGCA AGAATGTGAT TGGAGGTTTT GAATGCACCT GCGAGGAGGG ATTTGAGCCC GGTCCAATGA TGACATGTGA AGATATAAAT GAATGTGCCC AGAATCCTCT GCTCTGTGCC TTCCGATGTG TGAACACTTA TGGGTCATAT GAATGCAAAT GTCCCGTGGG ATATGTGCTC AGAGAAGACC GTAGGATGTG CAAAGATGAG GATGAGTGTG AAGAGGGAAA ACATGACTGT ACTGAAAAAC AAATGGAATG CAAGAACCTC ATTGGCACAT ATATGTGCAT CTGTGGACCC GGGTATCAGC GGAGACCTGA TGGAGAAGGC TGTGTAGATG AGAATGAATG TCAGACGAAG CCAGGGATCT GTGAGAATGG GCGCTGCCTC AACACCCGTG GGAGCTACAC CTGTGAGTGT AATGATGGGT TTACCGCCAG CCCCAACCAG GACGAGTGCC TTGACAATCG GGAAGGGTAC TGCTTCACAG AGGTGCTACA AAACATGTGT CAGATCGGCT CCAGCAACAG GAACCCCGTC ACCAAATCGG AATGCTGCTG TGACGGAGGG AGAGGCTGGG GTCCCCACTG TGAGATCTGC CCTTTCCAGG GGACTGTGGC TTTCAAGAAA CTCTGTCCCC ATGGCCGAGG ATTCATGACC AATGGAGCAG ATATCGATGA ATGCAAGGTT ATTCACGATG TTTGCCGAAA TGGGGAATGT GTCAATGACA GAGGATCATA TCATTGCATT TGTAAAACTG GGTACACTCC AGATATAACT GGGACTTCCT GTGTAGATCT GAACGAGTGC AACCAGGCTC CCAAACCCTG CAATTTTATC TGCAAAAACA CAGAAGGGAG TTACCAGTGT TCATGCCCGA AAGGCTACAT TCTGCAAGAG GATGGAAGGA GCTGCAAAGA TCTTGATGAG TGTGCAACCA AGCAACACAA CTGCCAGTTC CTATGTGTTA ACACCATTGG CGGCTTCACA TGCAAATGTC CTCCCGGATT TACCCAACAC CATACGTCCT GCATTGATAA CAATGAATGC ACCTCTGACA TCAATCTGTG CGGGTCTAAG GGCATTTGCC AGAACACTCC TGGAAGCTTC ACCTGTGAAT GCCAGCGGGG ATTCTCACTT GATCAGACCG GCTCCAGCTG TGAAGACGTG GACGAGTGTG AGGGTAACCA CCGCTGCCAG CATGGCTGCC AGAACATCAT TGGGGGCTAC AGGTGCAGCT GCCCCCAGGG CTACCTCCAG CACTACCAGT GGAACCAGTG TGTTGATGAA AACGAATGCC TCAGCGCTCA CATCTGCGGA GGAGCCTCCT GTCACAACAC CCTGGGGAGC TACAAGTGCA TGTGTCCCGC CGGCTTCCAG TATGAACAGT TCAGTGGAGG ATGCCAAGAC ATCAATGAAT GTGGCTCTGC GCAGGCCCCC TGCAGCTATG GCTGTTCCAA TACCGAGGGC GGTTACCTGT GTGGCTGTCC ACCTGGTTAC TTCCGCATAG GCCAAGGGCA CTGTGTTTCT GGAATGGGCA TGGGCCGAGG AAACCCAGAG CCACCTGTCA GTGGTGAAAT GGATGACAAT TCACTCTCCC CAGAGGCTTG TTACGAGTGT AAGATCAATG GCTACCCCAA ACGGGGCAGG AAACGGAGAA GCACAAACGA AACTGATGCC TCCAATATCG AGGATCAGTC TGAGACAGAA GCCAATGTGA GTCTTGCAAG TTGGGATGTT GAGAAGACAG CCATCTTTGC TTTCAATATT TCCCACGTCA GTAACAAGGT TCGAATCCTA GAACTCCTTC CAGCTCTTAC AACTCTGACG AATCACAACA GATACTTGAT CGAATCTGGA AATGAAGATG GCTTCTTTAA AATCAACCAA AAGGAAGGGA TCAGCTACCT CCACTTCACA AAGAAGAAGC CAGTGGCTGG AACCTATTCA TTACAAATCA GTAGTACTCC ACTTTATAAA AAGAAAGAAC TTAACCAACT AGAAGACAAA TATGACAAAG ACTACCTCAG TGGTGAACTG GGTGATAATC TGAAGATGAA AATCCAGGTT TTGCTTCATC TCGAGTACCC ATACGACGTA CCTGATTATG CAACTTATCC TTATGATGTA CCTGATTATG CTAGCTATCC TTATGATGTC CCAGACTACG CATAGGGATC CCTGCGATAT CCAGCGCGGC CGCGAAGGAT CTGCGATCGC TCCGGTGCCC GTCAGTGGGC AGAGCGCACA TCGCCCACAG TCCCCGAGAA GTTGGGGGGA GGGGTCGGCA ATTGAACGGG TGCCTAGAGA AGGTGGCGCG GGGTAAACTG GGAAAGTGAT GTCGTGTACT GGCTCCGCCT TTTTCCCGAG GGTGGGGGAG AACCGTATAT AAGTGCAGTA GTCGCCGTGA ACGTTCTTTT TCGCAACGGG TTTGCCGCCA GAACACAGCT GAAGCTTCGA GGGGCTCGCA TCTCTCCTTC ACGCGCCCGC CGCCCTACCT GAGGCCGCCA TCCACGCCGG TTGAGTCGCG TTCTGCCGCC TCCCGCCTGT GGTGCCTCCT GAACTGCGTC CGCCGTCTAG GTAAGTTTAA AGCTCAGGTC GAGACCGGGC CTTTGTCCGG CGCTCCCTTG GAGCCTACCT AGACTCAGCC GGCTCTCCAC GCTTTGCCTG ACCCTGCTTG CTCAACTCTA CGTCTTTGTT TCGTTTTCTG TTCTGCGCCG TTACAGATCC AAGCTGTGAC CGGCGCCTAC GCTAGACGCC ACCATGGAGA GCGACGAGAG CGGCCTGCCC GCCATGGAGA TCGAGTGCCG CATCACCGGC ACCCTGAACG GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG AGGGCACCCC CAAGCAGGGC CGCATGACCA ACAAGATGAA GAGCACCAAA GGCGCCCTGA CCTTCAGCCC CTACCTGCTG AGCCACGTGA TGGGCTACGG CTTCTACCAC TTCGGCACCT ACCCCAGCGG CTACGAGAAC CCCTTCCTGC ACGCCATCAA CAACGGCGGC TACACCAACA CCCGCATCGA GAAGTACGAG GACGGCGGCG TGCTGCACGT GAGCTTCAGC TACCGCTACG AGGCCGGCCG CGTGATCGGC GACTTCAAGG TGGTGGGCAC CGGCTTCCCC GAGGACAGCG TGATCTTCAC CGACAAGATC ATCCGCAGCA ACGCCACCGT GGAGCACCTG CACCCCATGG GCGATAACGT GCTGGTGGGC AGCTTCGCCC GCACCTTCAG CCTGCGCGAC GGCGGCTACT ACAGCTTCGT GGTGGACAGC CACATGCACT TCAAGAGCGC CATCCACCCC AGCATCCTGC AGAACGGGGG CCCCATGTTC GCCTTCCGCC GCGTGGAGGA GCTGCACAGC AACACCGAGC TGGGCATCGT GGAGTACCAG CACGCCTTCA AGACCCCCAT CGCCTTCGCC AGATCCCGCG CTCAGTCGTC CAATTCTGCC GTGGACGGCA CCGCCGGACC CGGCTCCACC GGATCTCGCG AGGGCAGAGG AAGTCTTCTA ACATGCGGTG ACGTGGAGGA GAATCCCGGC CCTATGACCG AGTACAAGCC CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGAAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAGC TGACGTCCTT TCCATGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTCCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGC CGCCTCCCCG CCTGGTACCT TTAAGACCAA TGACTTACAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG GGACTGGAAG GGCTAATTCA CTCCCAACGA AAATAAGATC TGCTTTTTGC TTGTACTGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT AGTAGTTCAT GTCATCTTAT TATTCAGTAT TTATAACTTG CAAAGAAATG AATATCAGAG AGTGAGAGGA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GCTCTAGCTA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGAC TTTTGCAGAG ACGGCCCAAA TTCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG CCAAGCTG
