pFUSE-CA4(human)-hIgG1-Fc2
$113.00
In stock
SKU
PL38711
100μg liquid plasmid(Instantly):$134
| name | pFUSE-CA4(human)-hIgG1-Fc2 | another name | apFUSE-CA4(human)-hIgG1-Fc2 |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression,Antibody expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Zeo |
| Selective marker | Zeo | Fluorescent labeling | |
| Promoter | EF1a | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGATCTGCGA TCGCTCCGGT GCCCGTCAGT GGGCAGAGCG CACATCGCCC ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC GGCAATTGAA CGGGTGCCTA GAGAAGGTGG CGCGGGGTAA ACTGGGAAAG TGATGTCGTG TACTGGCTCC GCCTTTTTCC CGAGGGTGGG GGAGAACCGT ATATAAGTGC AGTAGTCGCC GTGAACGTTC TTTTTCGCAA CGGGTTTGCC GCCAGAACAC AGCTGAAGCT TCGAGGGGCT CGCATCTCTC CTTCACGCGC CCGCCGCCCT ACCTGAGGCC GCCATCCACG CCGGTTGAGT CGCGTTCTGC CGCCTCCCGC CTGTGGTGCC TCCTGAACTG CGTCCGCCGT CTAGGTAAGT TTAAAGCTCA GGTCGAGACC GGGCCTTTGT CCGGCGCTCC CTTGGAGCCT ACCTAGACTC AGCCGGCTCT CCACGCTTTG CCTGACCCTG CTTGCTCAAC TCTACGTCTT TGTTTCGTTT TCTGTTCTGC GCCGTTACAG ATCCAAGCTG TGACCGGCGC CTACCTGAGA TCACCGGTGA ATTCGATATC TCGAGCGCCA CCATGGAGAT GCTGCTGGCG CTCCTGGCCC TCTCCGCGGC GCGGCCATCG GCCAGTGCAG AGTCACACTG GTGCTACGAG GTTCAAGCCG AGTCCTCCAA CTACCCCTGC TTGGTGCCAG TCAAGTGGGG TGGAAACTGC CAGAAGGACC GCCAGTCCCC CATCAACATC GTCACCACCA AGGCAAAGGT GGACAAAAAA CTGGGACGCT TCTTCTTCTC TGGCTACGAT AAGAAGCAAA CGTGGACTGT CCAAAATAAC GGGCACTCAG TGATGATGTT GCTGGAGAAC AAGGCCAGCA TTTCTGGAGG AGGACTGCCT GCCCCATACC AGGCCAAACA GTTGCACCTG CACTGGTCCG ACTTGCCATA TAAGGGCTCG GAGCACAGCC TCGATGGGGA GCACTTTGCC ATGGAGATGC ACATAGTACA TGAGAAAGAG AAGGGGACAT CGAGGAATGT GAAAGAGGCC CAGGACCCTG AAGACGAAAT TGCGGTGCTG GCCTTTCTGG TGGAGGCTGG AACCCAGGTG AACGAGGGCT TCCAGCCACT GGTGGAGGCA CTGTCTAATA TCCCCAAACC TGAGATGAGC ACTACGATGG CAGAGAGCAG CCTGTTGGAC CTGCTCCCCA AGGAGGAGAA ACTGAGGCAC TACTTCCGCT ACCTGGGCTC ACTCACCACA CCGACCTGCG ATGAGAAGGT CGTCTGGACT GTGTTCCGGG AGCCCATTCA GCTTCACAGA GAACAGATCC TGGCATTCTC TCAGAAGCTG TACTACGACA AGGAACAGAC AGTGAGCATG AAGGACAATG TCAGGCCCCT GCAGCAGCTG GGGCAGCGCA CGGTGATAAA GTCCGGGCTT GAAGTCCTCT TTCAGGGACC CACTCACACA TGCCCACCGT GCCCAGCACC TGAACTCCTG GGGGGACCGT CAGTCTTCCT CTTCCCCCCA AAACCCAAGG ACACCCTCAT GATCTCCCGG ACCCCTGAGG TCACATGCGT GGTGGTGGAC GTGAGCCACG AAGACCCTGA GGTCAAGTTC AACTGGTACG TGGACGGCGT GGAGGTGCAT AATGCCAAGA CAAAGCCGCG GGAGGAGCAG TACAACAGCA CGTACCGTGT GGTCAGCGTC CTCACCGTCC TGCACCAGGA CTGGCTGAAT GGCAAGGAGT ACAAGTGCAA GGTCTCCAAC AAAGCCCTCC CAGCCCCCAT CGAGAAAACC ATCTCCAAAG CCAAAGGGCA GCCCCGAGAA CCACAGGTGT ACACCCTGCC CCCATCCCGG GAGGAGATGA CCAAGAACCA GGTCAGCCTG ACCTGCCTGG TCAAAGGCTT CTATCCCAGC GACATCGCCG TGGAGTGGGA GAGCAATGGG CAGCCGGAGA ACAACTACAA GACCACGCCT CCCGTGCTGG ACTCCGACGG CTCCTTCTTC CTCTACAGCA AGCTCACCGT GGACAAGAGC AGGTGGCAGC AGGGGAACGT CTTCTCATGC TCCGTGATGC ACGAGGCTCT GCACAACCAC TACACGCAGA AGAGCCTCTC CCTGTCTCCG GGTAAATGAG TGCTAGCTGG CCAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTATGGAATT AATTCTAAAA TACAGCATAG CAAAACTTTA ACCTCCAAAT CAAGCCTCTA CTTGAATCCT TTTCTGAGGG ATGAATAAGG CATAGGCATC AGGGGCTGTT GCCAATGTGC ATTAGCTGTT TGCAGCCTCA CCTTCTTTCA TGGAGTTTAA GATATAGTGT ATTTTCCCAA GGTTTGAACT AGCTCTTCAT TTCTTTATGT TTTAAATGCA CTGACCTCCC ACATTCCCTT TTTAGTAAAA TATTCAGAAA TAATTTAAAT ACATCATTGC AATGAAAATA AATGTTTTTT ATTAGGCAGA ATCCAGATGC TCAAGGCCCT TCATAATATC CCCCAGTTTA GTAGTTGGAC TTAGGGAACA AAGGAACCTT TAATAGAAAT TGGACAGCAA GAAAGCGAGC TTCTAGCTTA TCCTCAGTCC TGCTCCTCTG CCACAAAGTG CACGCAGTTG CCGGCCGGGT CGCGCAGGGC GAACTCCCGC CCCCACGGCT GCTCGCCGAT CTCGGTCATG GCCGGCCCGG AGGCGTCCCG GAAGTTCGTG GACACGACCT CCGACCACTC GGCGTACAGC TCGTCCAGGC CGCGCACCCA CACCCAGGCC AGGGTGTTGT CCGGCACCAC CTGGTCCTGG ACCGCGCTGA TGAACAGGGT CACGTCGTCC CGGACCACAC CGGCGAAGTC GTCCTCCACG AAGTCCCGGG AGAACCCGAG CCGGTCGGTC CAGAACTCGA CCGCTCCGGC GACGTCGCGC GCGGTGAGCA CCGGAACGGC ACTGGTCAAC TTGGCCATGA TGGCTCCTCC TGTCAGGAGA GGAAAGAGAA GAAGGTTAGT ACAATTGCTA TAGTGAGTTG TATTATACTA TGCAGATATA CTATGCCAAT GATTAATTGT CAAACTAGGG CTGCAGGGTT CATAGTGCCA CTTTTCCTGC ACTGCCCCAT CTCCTGCCCA CCCTTTCCCA GGCATAGACA GTCAGTGACT TACCAAACTC ACAGGAGGGA GAAGGCAGAA GCTTGAGACA GACCCGCGGG ACCGCCGAAC TGCGAGGGGA CGTGGCTAGG GCGGCTTCTT TTATGGTGCG CCGGCCCTCG GAGGCAGGGC GCTCGGGGAG GCCTAGCGGC CAATCTGCGG TGGCAGGAGG CGGGGCCGAA GGCCGTGCCT GACCAATCCG GAGCACATAG GAGTCTCAGC CCCCCGCCCC AAAGCAAGGG GAAGTCACGC GCCTGTAGCG CCAGCGTGTT GTGAAATGGG GGCTTGGGGG GGTTGGGGCC CTGACTAGTC AAAACAAACT CCCATTGACG TCAATGGGGT GGAGACTTGG AAATCCCCGT GAGTCAAACC GCTATCCACG CCCATTGATG TACTGCCAAA ACCGCATCAT CATGGTAATA GCGATGACTA ATACGTAGAT GTACTGCCAA GTAGGAAAGT CCCATAAGGT CATGTACTGG GCATAATGCC AGGCGGGCCA TTTACCGTCA TTGACGTCAA TAGGGGGCGT ACTTGGCATA TGATACACTT GATGTACTGC CAAGTGGGCA GTTTACCGTA AATACTCCAC CCATTGACGT CAATGGAAAG TCCCTATTGG CGTTACTATG GGAACATACG TCATTATTGA CGTCAATGGG CGGGGGTCGT TGGGCGGTCA GCCAGGCGGG CCATTTACCG TAAGTTATGT AACGCCTGCA GGTTAATTAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGGCT AGTTAATTAA CATTTAAATC AGCGGCCGCA ATAAAATATC TTTATTTTCA TTACATCTGT GTGTTGGTTT TTTGTGTGAA TCGTAACTAA CATACGCTCT CCATCAAAAC AAAACGAAAC AAAACAAACT AGCAAAATAG GCTGTCCCCA GTGCAAGTGC AGGTGCCAGA ACATTTCTCT ATCGAA
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