NM_004448.4;c-ERB-2; c-ERB2; CD340; HER-2; HER-2/neu;HER2; MLN 19; MLN-19; NEU; NGL; p185(erbB2); TKR1; VSCN2
100μg liquid plasmid(Instantly):$120
| name | pLV3-CMV-ERBB2(human)-EGFP-Puro | another name | apLV3-CMV-ERBB2(human)-EGFP-Puro |
| Insert Size (bp) | 3765bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCGCGCAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGA GCTGCAAGCT TAATGTAGTC TTATGCAATA CTCTTGTAGT CTTGCAACAT GGTAACGATG AGTTAGCAAC ATGCCTTACA AGGAGAGAAA AAGCACCGTG CATGCCGATT GGTGGAAGTA AGGTGGTACG ATCGTGCCTT ATTAGGAAGG CAACAGACGG GTCTGACATG GATTGGACGA ACCACTGAAT TGCCGCATTG CAGAGATATT GTATTTAAGT GCCTAGCTCG ATACATAAAC GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCGCTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCG TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTTGG AATCACACGA CCTGGATGGA GTGGGACAGA GAAATTAACA ATTACACAAG CTTAATACAC TCCTTAATTG AAGAATCGCA AAACCAGCAA GAAAAGAATG AACAAGAATT ATTGGAATTA GATAAATGGG CAAGTTTGTG GAATTGGTTT AACATAACAA ATTGGCTGTG GTATATAAAA TTATTCATAA TGATAGTAGG AGGCTTGGTA GGTTTAAGAA TAGTTTTTGC TGTACTTTCT ATAGTGAATA GAGTTAGGCA GGGATATTCA CCATTATCGT TTCAGACCCA CCTCCCAACC CCGAGGGGAC CCGACAGGCC CGAAGGAATA GAAGAAGAAG GTGGAGAGAG AGACAGAGAC AGATCCATTC GATTAGTGAA CGGATCTCGA CGGTATCGGT TAACTTTTAA AAGAAAAGGG GGGATTGGGG GGTACAGTGC AGGGGAAAGA ATAGTAGACA TAATAGCAAC AGACATACAA ACTAAAGAAT TACAAAAACA AATTACAAAA TTCAAAATTT TATCGATAAG CTTGGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGA CTCTAGAGCC ACCATGGAGC TGGCGGCCTT GTGCCGCTGG GGGCTCCTCC TCGCCCTCTT GCCCCCCGGA GCCGCGAGCA CCCAAGTGTG CACCGGCACA GACATGAAGC TGCGGCTCCC TGCCAGTCCC GAGACCCACC TGGACATGCT CCGCCACCTC TACCAGGGCT GCCAGGTGGT GCAGGGAAAC CTGGAACTCA CCTACCTGCC CACCAATGCC AGCCTGTCCT TCCTGCAGGA TATCCAGGAG GTGCAGGGCT ACGTGCTCAT CGCTCACAAC CAAGTGAGGC AGGTCCCACT GCAGAGGCTG CGGATTGTGC GAGGCACCCA GCTCTTTGAG GACAACTATG CCCTGGCCGT GCTAGACAAT GGAGACCCGC TGAACAATAC CACCCCTGTC ACAGGGGCCT CCCCAGGAGG CCTGCGGGAG CTGCAGCTTC GAAGCCTCAC AGAGATCTTG AAAGGAGGGG TCTTGATCCA GCGGAACCCC CAGCTCTGCT ACCAGGACAC GATTTTGTGG AAGGACATCT TCCACAAGAA CAACCAGCTG GCTCTCACAC TGATAGACAC CAACCGCTCT CGGGCCTGCC ACCCCTGTTC TCCGATGTGT AAGGGCTCCC GCTGCTGGGG AGAGAGTTCT GAGGATTGTC AGAGCCTGAC GCGCACTGTC TGTGCCGGTG GCTGTGCCCG CTGCAAGGGG CCACTGCCCA CTGACTGCTG CCATGAGCAG TGTGCTGCCG GCTGCACGGG CCCCAAGCAC TCTGACTGCC TGGCCTGCCT CCACTTCAAC CACAGTGGCA TCTGTGAGCT GCACTGCCCA GCCCTGGTCA CCTACAACAC AGACACGTTT GAGTCCATGC CCAATCCCGA GGGCCGGTAT ACATTCGGCG CCAGCTGTGT GACTGCCTGT CCCTACAACT ACCTTTCTAC GGACGTGGGA TCCTGCACCC TCGTCTGCCC CCTGCACAAC CAAGAGGTGA CAGCAGAGGA TGGAACACAG CGGTGTGAGA AGTGCAGCAA GCCCTGTGCC CGAGTGTGCT ATGGTCTGGG CATGGAGCAC TTGCGAGAGG TGAGGGCAGT TACCAGTGCC AATATCCAGG AGTTTGCTGG CTGCAAGAAG ATCTTTGGGA GCCTGGCATT TCTGCCGGAG AGCTTTGATG GGGACCCAGC CTCCAACACT GCCCCGCTCC AGCCAGAGCA GCTCCAAGTG TTTGAGACTC TGGAAGAGAT CACAGGTTAC CTATACATCT CAGCATGGCC GGACAGCCTG CCTGACCTCA GCGTCTTCCA GAACCTGCAA GTAATCCGGG GACGAATTCT GCACAATGGC GCCTACTCGC TGACCCTGCA AGGGCTGGGC ATCAGCTGGC TGGGGCTGCG CTCACTGAGG GAACTGGGCA GTGGACTGGC CCTCATCCAC CATAACACCC ACCTCTGCTT CGTGCACACG GTGCCCTGGG ACCAGCTCTT TCGGAACCCG CACCAAGCTC TGCTCCACAC TGCCAACCGG CCAGAGGACG AGTGTGTGGG CGAGGGCCTG GCCTGCCACC AGCTGTGCGC CCGAGGGCAC TGCTGGGGTC CAGGGCCCAC CCAGTGTGTC AACTGCAGCC AGTTCCTTCG GGGCCAGGAG TGCGTGGAGG AATGCCGAGT ACTGCAGGGG CTCCCCAGGG AGTATGTGAA TGCCAGGCAC TGTTTGCCGT GCCACCCTGA GTGTCAGCCC CAGAATGGCT CAGTGACCTG TTTTGGACCG GAGGCTGACC AGTGTGTGGC CTGTGCCCAC TATAAGGACC CTCCCTTCTG CGTGGCCCGC TGCCCCAGCG GTGTGAAACC TGACCTCTCC TACATGCCCA TCTGGAAGTT TCCAGATGAG GAGGGCGCAT GCCAGCCTTG CCCCATCAAC TGCACCCACT CCTGTGTGGA CCTGGATGAC AAGGGCTGCC CCGCCGAGCA GAGAGCCAGC CCTCTGACGT CCATCATCTC TGCGGTGGTT GGCATTCTGC TGGTCGTGGT CTTGGGGGTG GTCTTTGGGA TCCTCATCAA GCGACGGCAG CAGAAGATCC GGAAGTACAC GATGCGGAGA CTGCTGCAGG AAACGGAGCT GGTGGAGCCG CTGACACCTA GCGGAGCGAT GCCCAACCAG GCGCAGATGC GGATCCTGAA AGAGACGGAG CTGAGGAAGG TGAAGGTGCT TGGATCTGGC GCTTTTGGCA CAGTCTACAA GGGCATCTGG ATCCCTGATG GGGAGAATGT GAAAATTCCA GTGGCCATCA AAGTGTTGAG GGAAAACACA TCCCCCAAAG CCAACAAAGA AATCTTAGAC GAAGCATACG TGATGGCTGG TGTGGGCTCC CCATATGTCT CCCGCCTTCT GGGCATCTGC CTGACATCCA CGGTGCAGCT GGTGACACAG CTTATGCCCT ATGGCTGCCT CTTAGACCAT GTCCGGGAAA ACCGCGGACG CCTGGGCTCC CAGGACCTGC TGAACTGGTG TATGCAGATT GCCAAGGGGA TGAGCTACCT GGAGGATGTG CGGCTCGTAC ACAGGGACTT GGCCGCTCGG AACGTGCTGG TCAAGAGTCC CAACCATGTC AAAATTACAG ACTTCGGGCT GGCTCGGCTG CTGGACATTG ACGAGACAGA GTACCATGCA GATGGGGGCA AGGTGCCCAT CAAGTGGATG GCGCTGGAGT CCATTCTCCG CCGGCGGTTC ACCCACCAGA GTGATGTGTG GAGTTATGGT GTGACTGTGT GGGAGCTGAT GACTTTTGGG GCCAAACCTT ACGATGGGAT CCCAGCCCGG GAGATCCCTG ACCTGCTGGA AAAGGGGGAG CGGCTGCCCC AGCCCCCCAT CTGCACCATT GATGTCTACA TGATCATGGT CAAATGTTGG ATGATTGACT CTGAATGTCG GCCAAGATTC CGGGAGTTGG TGTCTGAATT CTCCCGCATG GCCAGGGACC CCCAGCGCTT TGTGGTCATC CAGAATGAGG ACTTGGGCCC AGCCAGTCCC TTGGACAGCA CCTTCTACCG CTCACTGCTG GAGGACGATG ACATGGGGGA CCTGGTGGAT GCTGAGGAGT ATCTGGTACC CCAGCAGGGC TTCTTCTGTC CAGACCCTGC CCCGGGCGCT GGGGGCATGG TCCACCACAG GCACCGCAGC TCATCTACCA GGAGTGGCGG TGGGGACCTG ACACTAGGGC TGGAGCCCTC TGAAGAGGAG GCCCCCAGGT CTCCACTGGC ACCCTCCGAA GGGGCTGGCT CCGATGTATT TGATGGTGAC CTGGGAATGG GGGCAGCCAA GGGGCTGCAA AGCCTCCCCA CACATGACCC CAGCCCTCTA CAGCGGTACA GTGAGGACCC CACAGTACCC CTGCCCTCTG AGACTGATGG CTACGTTGCC CCCCTGACCT GCAGCCCCCA GCCTGAATAT GTGAACCAGC CAGATGTTCG GCCCCAGCCC CCTTCGCCCC GAGAGGGCCC TCTGCCTGCT GCCCGACCTG CTGGTGCCAC TCTGGAAAGG CCCAAGACTC TCTCCCCAGG GAAGAATGGG GTCGTCAAAG ACGTTTTTGC CTTTGGGGGT GCCGTGGAGA ACCCCGAGTA CTTGACACCC CAGGGAGGAG CTGCCCCTCA GCCCCACCCT CCTCCTGCCT TCAGCCCAGC CTTCGACAAC CTCTATTACT GGGACCAGGA CCCACCAGAG CGGGGGGCTC CACCCAGCAC CTTCAAAGGG ACACCTACGG CAGAGAACCC AGAGTACCTG GGTCTGGACG TGCCAGTGCT CGAGGGCGGT GGAGGCAGTG GTGGAGGCGG TAGCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCACCCAGT CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GTAACGGGAT CCGGACTAGT TAACGCGTCG ACCAGTGGTC GACCCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTTA CCATGACCGA GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GACGCCCGCC CCACGACCCG CAGCGCCCGA CCGAAAGGAG CGCACGACCC CATGCATCGC CAGTCGACAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAGCTGACG TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCCTG GAATTCGAGC TCGGTACCTT TAAGACCAAT GACTTACAAG GCAGCTGTAG ATCTTAGCCA CTTTTTAAAA GAAAAGGGGG GACTGGAAGG GCTAATTCAC TCCCAACGAA GACAAGATCT GCTTTTTGCT TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGTA GTAGTTCATG TCATCTTATT ATTCAGTATT TATAACTTGC AAAGAAATGA ATATCAGAGA GTGAGAGGAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG CTCTAGCTAT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGG ACGTACCCAA TTCGCCCTAT AGTGAGTCGT ATTACGCGCG CTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGCTT ACAATTTAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AG
