pTol2-ZBactin-AFPIII(Antarctic eel pout)-EGFP
$99.00
In stock
SKU
PL38689
100μg liquid plasmid(Instantly):$120
| name | pTol2-ZBactin-AFPIII(Antarctic eel pout)-EGFP | another name | apTol2-ZBactin-AFPIII(Antarctic eel pout)-EGFP |
| Insert Size (bp) | 258bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression,Transpose | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Neo/G418 | Fluorescent labeling | Green |
| Promoter | ZBactin | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAGTTATTAA TAGAGGTGTA AAGTACTTGA GTAATTTTAC TTGATTACTG TACTTAAGTA TTATTTTTGG GGATTTTTAC TTTACTTGAG TACAATTAAA AATCAATACT TTTACTTTTA CTTAATTACA TTTTTTTAGA AAAAAAAGTA CTTTTTACTC CTTACAATTT TATTTACAGT CAAAAAGTAC TTATTTTTTG GAGATCACTT CATTCTATTT TCCCTTGCTA TTAGCTAGCG CTACCGGACT CAGATCTCGA GCGTTTACTC TGAGAAACTT CCATATTTTA GAGGTAAAAT GCTCAGAATT GTAAAAAGTC TTGTCAAAAT ACTTGGAATT TGCCAAAAAC AAAATAAAAG TCACATCACC TCATTGTCCT TGGTATCAGC AAGCACTTTT AACACAATCA GCTGTTTTTG TTTATAACTC CCACTGAAGG CCATGTGCAT TACTCCCAAG TTACACAGTA AAAAGTCACA TGTAGTTTAT CATGATCACT GCTGCTCAAG CACAGCTTTA TCTGTTATTC AGTGTGGCAA TATCTGAAAT GTGTGCAAAA TATTATTTAT TGTAGCTGAT CACCAACACT TTCAGTGTTT TTTTTTTCTA CTTTTAATGA GAATTATGTT CTTAGGATTT ATCCTTATAA ACCTCTTTAA AAAGAAATGT TATGTCTGCA TAGACACGAC ATGAAATTAT GAACAGAAAA TTTAACTATT TAAGATCAAG ACTTGACATA TAACATTTCT AATGAACATT AAACCTGAGG TAGGCCCACT TGCAAAAATG TAACTGCTAT CACTCACTTT ATACATCATT GTGTAACTTG TACACACAAA AATAGTGAAG CAATGTGGCA TGGAATGCAG GTCCCATTCA TTCAAAGTTA TTAAAGCACT TTCTTAAAGT GCATTACTAC TGAGCTAAGA CCAGCTAGTT GCTCATACAA CATACTACAA GTAGTGTCAT CCTAATTTGG ATAAAAAAAA TGTCCATTTT ATACATGGAA AAGTACTCAT TAGATCCCCC ATTGTTTGTA TTACGGTATT TCGTGAACAC AAGAGGTAAA TGACCTACAG AGCTGCTGTT GTGTTAGATT GAAAACACAA CACAGGATCA TGGAGGCATC CATCATTATG ACGCATTCTG CCACTTGTAA CTCTGCACAG ACTTTTTGAA AAGTTTAATT GAGTCTTGGC GTTGTCGCAG AAAAATACGA AAAATTCTGT GTTAAAATTT GCAAATAAAA ATTCCAGTTT TATAGGAAAT CGCCATGGCC TTGTTCTTTA ACCAGTTAAG CTTCCCCTTC TTTCACTCTC AAGTTGCAAG AAGCAAGTGT AGCAATGTGC ACGCGACAGC CGGGTGTGTG ACGCTGGACC AATCAGAGCA CAGAGCTCCG AAAGTTTACC TTTTATGGCT AGAGCCGGGT ATGTGCCGTC ATATAAAAGA GCGCGCCCAG CTTTTCAGCC TCACTTTGAG CTCCTCCACA CGCAGCTAGT GCGGAATATC ATCTGCTTGT AACCCATTCT CTTAAGTCGA CAACCCCCCA AACCCAAGGT GAGTTGGTTT TACAGTTTTT AAGCGTTTAA TTATAGTTTC TATCTATTAA TGTTAAGTAA AATGTGTAAA TGGATAAATA TGATTTGATG AAGTCTTTAG ACAGTTCATA ACGGTCTCTT TGTAGTTATC TACTAGGTTT GATTTATAAG TGGTGGTTCT TTTGATAATC TATGTTACAT TTAATAAATA TTGGCTGTAT CTTAACTTTT TTGTAGCGTT AGTGGATGTG GCAGGTGAGA ATGTCGGTAA TTTAACGTGA CCAGTCGTAG GCACGACATT GAACATTGAA TAGGCCGGTG TGAAATGGGT GTTAAGTCTT AATATAAAGT TGTGTTCTGG CTAATCTCTC TTTAACAGCC ATAAATGTGA TTTTTAACTA CAGTTTTGAG GGTGCTGGTG AGCATGTTGC ACACTTGATG GATAGCCGGC ATGGGAAGTT CTTTGTGCAG GCAGTGCTGC AGCAGGGTGT GACCTACTTT AGCTAGCCGG CTAACCAGCT CTCATCTGAT GTAACGTAAA CCCCATCAAT CTAACATCGG CTCATTCGTG CTTTATAAGA TCGAAGATCT TGTTATTAGT AACGGGTATT GGCATTTAGT TTTAACTTGG TTAATTTTCT TTTTGTTATG TGTTCTATTT AAATGTAGCC TGCAGCCCCC TCTGTCTTTA ATGAAGCTGA AGATTAACGT TAACGTTAGT GAAGTGTTGT ATCCGCTATG AAAGATTTGA ATTTTAGAGG TGTGGTCCAC TTTAATAACG GCTGCGGAGT TCTCTGGCTG CACTTCAGCC GCTGTGACTG TCGAAGCAGA GCCCATCAGT TTTTCAGGAT CTCGGTGATT TTTGAGGCTA GACTATGAAC TGAACCGACT GAGAGTGACC GTCCAGAAAT GCTTTAGTGT GAAGTCTCCG CTCACGAGGA GTCCGTGGAC TGACTGCAGA TCTGTGACGC AGTAACACCC GGCGGCAGAC ACCCGTTGAA ATGCGGTTGT GTAATTGATC GCAGGCGAGG GTGGAAGAGG ATGTGAAACT TCATTTGTGT AAAATTTAGG GAGTGGTCCT GGTGTGATGA ATGTCGAAAT CTGTTCCTTT TTACTGAGCC CTACGACTCT GGCTGAGTGC CACACCGCCG GCAGCCGCAA AGCGGCTCAA ACCATTGCCT TTTATGGTAA TAATGAGAGA ATGCAGAGGG ACTTCCTTTG TCTGGCATAT CTGAGGCGCG CGCTGTCACT AGCGCCCACC AGCGGTCAGA CTGTAGAATG CAGAGCAAAA CAGGAAGTTG ACTCCAGATG GTCACATGCT TGCTGAAACG CCTCTCCCTG GCAGCAGTGC AATTCTAACA GTTTCCCTTT CTTTTACAGT TCAGCCGAAT TCTGCAGTCG ACGGTACCAG CCACCATGAA GTCAGTTGTT TTAACTGGTT TGCTGTTCGT CCTCCTTTGT GTCGACCACA TGAGTTCAGC CACCAAGTCC GTGGTGGCCA GCCAGCTGAT CCCCATAAAT ACTGCCCTGA CTCCAGCGAT GATGAAGGCG AAGGAAGTCA GCCCAAAGGG CATCCCTGCC GAGGAGATGT CCAAAATAGT GGGAATGCAA GTGAACAGGG CAGTGAATCT GGACGAAACC CTCATGCCAG ATATGGTGAA AACGTATCAG AAGGGATCCG GAGGTGGCAG CGGTGGCGGT CAGCTGTTGA ATTTTGACCT TCTTAAACTT GCGGGAGACG TCGAGTCCAA CCCTGGGCCC ACAACCATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTAAAGCG GCCGCGACTC TAGATCATAA TCAGCCATAC CACATTTGTA GAGGTTTTAC TTGCTTTAAA AAACCTCCCA CACCTCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA AGGCTTAAAC AAGAATCTCT AGTTTTCTTT CTTGCTTTTA CTTTTACTTC CTTAATACTC AAGTACAATT TTAATGGAGT ACTTTTTTAC TTTTACTCAA GTAAGATTCT AGCCAGATAC TTTTACTTTT AATTGAGTAA AATTTTCCCT AAGTACTTGT ACTTTCACTT GAGTAAAATT TTTGAGTACT TTTTACACCT CTGCTTAAGG CGTAAATTGT AAGCGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA TAAATCAAAA GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC AGGGCGATGG CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCACT AAATCGGAAC CCTAAAGGGA GCCCCCGATT TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC GGTCACGCTG CGCGTAACCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT CCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAG ATCGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC TAGGGGGAGG CTAACTGAAA CACGGAAGGA GACAATACCG GAAGGAACCC GCGCTATGAC GGCAATAAAA AGACAGAATA AAACGCACGG TGTTGGGTCG TTTGTTCATA AACGCGGGGT TCGGTCCCAG GGCTGGCACT CTGTCGATAC CCCACCGAGA CCCCATTGGG GCCAATACGC CCGCGTTTCT TCCTTTTCCC CACCCCACCC CCCAAGTTCG GGTGAAGGCC CAGGGCTCGC AGCCAACGTC GGGGCGGCAG GCCCTGCCAT AGCCTCAGGT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCATGCAT
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