pTol2-ZBactin-AFPIII(Atlantic wolffish)-EGFP
$99.00
In stock
SKU
PL38682
100μg liquid plasmid(Instantly):$120
| name | pTol2-ZBactin-AFPIII(Atlantic wolffish)-EGFP | another name | apTol2-ZBactin-AFPIII(Atlantic wolffish)-EGFP |
| Insert Size (bp) | 459bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression,Transpose | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Neo/G418 | Fluorescent labeling | Green |
| Promoter | ZBactin | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAGTTATTAA TAGAGGTGTA AAGTACTTGA GTAATTTTAC TTGATTACTG TACTTAAGTA TTATTTTTGG GGATTTTTAC TTTACTTGAG TACAATTAAA AATCAATACT TTTACTTTTA CTTAATTACA TTTTTTTAGA AAAAAAAGTA CTTTTTACTC CTTACAATTT TATTTACAGT CAAAAAGTAC TTATTTTTTG GAGATCACTT CATTCTATTT TCCCTTGCTA TTAGCTAGCG CTACCGGACT CAGATCTCGA GCGTTTACTC TGAGAAACTT CCATATTTTA GAGGTAAAAT GCTCAGAATT GTAAAAAGTC TTGTCAAAAT ACTTGGAATT TGCCAAAAAC AAAATAAAAG TCACATCACC TCATTGTCCT TGGTATCAGC AAGCACTTTT AACACAATCA GCTGTTTTTG TTTATAACTC CCACTGAAGG CCATGTGCAT TACTCCCAAG TTACACAGTA AAAAGTCACA TGTAGTTTAT CATGATCACT GCTGCTCAAG CACAGCTTTA TCTGTTATTC AGTGTGGCAA TATCTGAAAT GTGTGCAAAA TATTATTTAT TGTAGCTGAT CACCAACACT TTCAGTGTTT TTTTTTTCTA CTTTTAATGA GAATTATGTT CTTAGGATTT ATCCTTATAA ACCTCTTTAA AAAGAAATGT TATGTCTGCA TAGACACGAC ATGAAATTAT GAACAGAAAA TTTAACTATT TAAGATCAAG ACTTGACATA TAACATTTCT AATGAACATT AAACCTGAGG TAGGCCCACT TGCAAAAATG TAACTGCTAT CACTCACTTT ATACATCATT GTGTAACTTG TACACACAAA AATAGTGAAG CAATGTGGCA TGGAATGCAG GTCCCATTCA TTCAAAGTTA TTAAAGCACT TTCTTAAAGT GCATTACTAC TGAGCTAAGA CCAGCTAGTT GCTCATACAA CATACTACAA GTAGTGTCAT CCTAATTTGG ATAAAAAAAA TGTCCATTTT ATACATGGAA AAGTACTCAT TAGATCCCCC ATTGTTTGTA TTACGGTATT TCGTGAACAC AAGAGGTAAA TGACCTACAG AGCTGCTGTT GTGTTAGATT GAAAACACAA CACAGGATCA TGGAGGCATC CATCATTATG ACGCATTCTG CCACTTGTAA CTCTGCACAG ACTTTTTGAA AAGTTTAATT GAGTCTTGGC GTTGTCGCAG AAAAATACGA AAAATTCTGT GTTAAAATTT GCAAATAAAA ATTCCAGTTT TATAGGAAAT CGCCATGGCC TTGTTCTTTA ACCAGTTAAG CTTCCCCTTC TTTCACTCTC AAGTTGCAAG AAGCAAGTGT AGCAATGTGC ACGCGACAGC CGGGTGTGTG ACGCTGGACC AATCAGAGCA CAGAGCTCCG AAAGTTTACC TTTTATGGCT AGAGCCGGGT ATGTGCCGTC ATATAAAAGA GCGCGCCCAG CTTTTCAGCC TCACTTTGAG CTCCTCCACA CGCAGCTAGT GCGGAATATC ATCTGCTTGT AACCCATTCT CTTAAGTCGA CAACCCCCCA AACCCAAGGT GAGTTGGTTT TACAGTTTTT AAGCGTTTAA TTATAGTTTC TATCTATTAA TGTTAAGTAA AATGTGTAAA TGGATAAATA TGATTTGATG AAGTCTTTAG ACAGTTCATA ACGGTCTCTT TGTAGTTATC TACTAGGTTT GATTTATAAG TGGTGGTTCT TTTGATAATC TATGTTACAT TTAATAAATA TTGGCTGTAT CTTAACTTTT TTGTAGCGTT AGTGGATGTG GCAGGTGAGA ATGTCGGTAA TTTAACGTGA CCAGTCGTAG GCACGACATT GAACATTGAA TAGGCCGGTG TGAAATGGGT GTTAAGTCTT AATATAAAGT TGTGTTCTGG CTAATCTCTC TTTAACAGCC ATAAATGTGA TTTTTAACTA CAGTTTTGAG GGTGCTGGTG AGCATGTTGC ACACTTGATG GATAGCCGGC ATGGGAAGTT CTTTGTGCAG GCAGTGCTGC AGCAGGGTGT GACCTACTTT AGCTAGCCGG CTAACCAGCT CTCATCTGAT GTAACGTAAA CCCCATCAAT CTAACATCGG CTCATTCGTG CTTTATAAGA TCGAAGATCT TGTTATTAGT AACGGGTATT GGCATTTAGT TTTAACTTGG TTAATTTTCT TTTTGTTATG TGTTCTATTT AAATGTAGCC TGCAGCCCCC TCTGTCTTTA ATGAAGCTGA AGATTAACGT TAACGTTAGT GAAGTGTTGT ATCCGCTATG AAAGATTTGA ATTTTAGAGG TGTGGTCCAC TTTAATAACG GCTGCGGAGT TCTCTGGCTG CACTTCAGCC GCTGTGACTG TCGAAGCAGA GCCCATCAGT TTTTCAGGAT CTCGGTGATT TTTGAGGCTA GACTATGAAC TGAACCGACT GAGAGTGACC GTCCAGAAAT GCTTTAGTGT GAAGTCTCCG CTCACGAGGA GTCCGTGGAC TGACTGCAGA TCTGTGACGC AGTAACACCC GGCGGCAGAC ACCCGTTGAA ATGCGGTTGT GTAATTGATC GCAGGCGAGG GTGGAAGAGG ATGTGAAACT TCATTTGTGT AAAATTTAGG GAGTGGTCCT GGTGTGATGA ATGTCGAAAT CTGTTCCTTT TTACTGAGCC CTACGACTCT GGCTGAGTGC CACACCGCCG GCAGCCGCAA AGCGGCTCAA ACCATTGCCT TTTATGGTAA TAATGAGAGA ATGCAGAGGG ACTTCCTTTG TCTGGCATAT CTGAGGCGCG CGCTGTCACT AGCGCCCACC AGCGGTCAGA CTGTAGAATG CAGAGCAAAA CAGGAAGTTG ACTCCAGATG GTCACATGCT TGCTGAAACG CCTCTCCCTG GCAGCAGTGC AATTCTAACA GTTTCCCTTT CTTTTACAGT TCAGCCGAAT TCTGCAGTCG ACGGTACCAG CCACCATGAA GTCAGCTATT TTAACTGGTT TGCTTTTCGT CCTCCTTTGT GTCGACCACA TGAGTTCAGC CCACCAGGTG AGATATTCTT GCTCCACAAA TAAATATTCA AAGATGTGAG CCACAGTAAA ATTCAATTGT TTTCTGTTTA GAAAGACAGA GAACCTTTAA GAAGACATTT TTGAAATAGT TTTTTCAACT GTGCCATGAG AACAATAATA ATGTCTGACC TTTTATTTTC CATTCTTCAA GGCGTCCGTG GTGGCCACCC AGCTGATCCC CATAAATACT GCCCTGACTC TGGTGATGAT GACGACCAGG GTGGTCTTCC CAACGGGCAT CCCCGCCGAG GACATTCCCC GATTAGTCTC AATGCAAGTG AACAGGGCAG TGCCGATGGG CACAACCCTC ATGCCAGACA TGGTGAAATT TTACTGCCTC TGCGCGCCGA AGACCCAAAG ATGGGGATCC GGAGGTGGCA GCGGTGGCGG TCAGCTGTTG AATTTTGACC TTCTTAAACT TGCGGGAGAC GTCGAGTCCA ACCCTGGGCC CACAACCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAAGC GGCCGCGACT CTAGATCATA ATCAGCCATA CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA AACATAAAAT GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT AAGGCTTAAA CAAGAATCTC TAGTTTTCTT TCTTGCTTTT ACTTTTACTT CCTTAATACT CAAGTACAAT TTTAATGGAG TACTTTTTTA CTTTTACTCA AGTAAGATTC TAGCCAGATA CTTTTACTTT TAATTGAGTA AAATTTTCCC TAAGTACTTG TACTTTCACT TGAGTAAAAT TTTTGAGTAC TTTTTACACC TCTGCTTAAG GCGTAAATTG TAAGCGTTAA TATTTTGTTA AAATTCGCGT TAAATTTTTG TTAAATCAGC TCATTTTTTA ACCAATAGGC CGAAATCGGC AAAATCCCTT ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTGTTGT TCCAGTTTGG AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA AACCGTCTAT CAGGGCGATG GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGGG GTCGAGGTGC CGTAAAGCAC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA TGCGCCGCTA CAGGGCGCGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TCCTGAGGCG GAAAGAACCA GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA GATCGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CTAGGGGGAG GCTAACTGAA ACACGGAAGG AGACAATACC GGAAGGAACC CGCGCTATGA CGGCAATAAA AAGACAGAAT AAAACGCACG GTGTTGGGTC GTTTGTTCAT AAACGCGGGG TTCGGTCCCA GGGCTGGCAC TCTGTCGATA CCCCACCGAG ACCCCATTGG GGCCAATACG CCCGCGTTTC TTCCTTTTCC CCACCCCACC CCCCAAGTTC GGGTGAAGGC CCAGGGCTCG CAGCCAACGT CGGGGCGGCA GGCCCTGCCA TAGCCTCAGG TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCATGCAT
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