pNL4-3-HA-R-E-
$142.00
In stock
SKU
PL38634
100μg liquid plasmid(Instantly):$163
| name | pNL4-3-HA-R-E- | another name | apNL4-3-HA-R-E- |
| Insert Size (bp) | Plasmid host | Mammary cell,Lentiviral | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GAGCCTAAAG GTAAAAAATA AAATAATAAA AAATAAATAA AATCTAGCTC ACTCCTTCAC ATCAAAATGG AGATACAGCT GTTAGCATTA AATACCAAAT AACCCATCTT GTCCTCAATA ATTTTAAGCG CCTCTCTCCA CCACATCTAA CTCCTGTCAA AGGCATGTGC CCCTTCCGGG CGCTCTGCTG TGCTGCCAAC CAACTGGCAT GTGGACTCTG CAGGGTCCCT AACTGCCAAG CCCCACAGTG TGCCCTGAGG CTGCCCCTTC CTTCTAGCGG CTGCCCCCAC TCGGCTTTGC TTTCCCTAGT TTCAGTTACT TGCGTTCAGC CAAGGTCTGA AACTAGGTGC GCACAGAGCG GTAAGACTGC GAGAGAAAGA GACCAGCTTT ACAGGGGGTT TATCACAGTG CACCCTGACA GTCGTCAGCC TCACAGGGGG TTTATCACAT TGCACCCTGA CAGTCGTCAG CCTCACAGGG GGTTTATCAC AGTGCACCCT TACAATCATT CCATTTGATT CACAATTTTT TTAGTCTCTA CTGTGCCTAA CTTGTAAGTT AAATTTGATC AGAGGTGTGT TCCCAGAGGG GAAAACAGTA TATACAGGGT TCAGTACTAT CGCATTTCAG GCCTCCACCT GGGTCTTGGA ATGTGTCCCC CGAGGGGTGA TGACTACCTC AGTTGGATCT CCACAGGTCA CAGTGACACA AGATAACCAA GACACCTCCC AAGGCTACCA CAATGGGCCG CCCTCCACGT GCACATGGCC GGAGGAACTG CCATGTCGGA GGTGCAAGCA CACCTGCGCA TCAGAGTCCT TGGTGTGGAG GGAGGGACCA GCGCAGCTTC CAGCCATCCA CCTGATGAAC AGAACCTAGG GAAAGCCCCA GTTCTACTTA CACCAGGAAA GGCTGGAAGG GCTAATTTGG TCCCAAAAAA GACAAGAGAT CCTTGATCTG TGGATCTACC ACACACAAGG CTACTTCCCT GATTGGCAGA ACTACACACC AGGGCCAGGG ATCAGATATC CACTGACCTT TGGATGGTGC TTCAAGTTAG TACCAGTTGA ACCAGAGCAA GTAGAAGAGG CCAATGAAGG AGAGAACAAC AGCTTGTTAC ACCCTATGAG CCAGCATGGG ATGGAGGACC CGGAGGGAGA AGTATTAGTG TGGAAGTTTG ACAGCCTCCT AGCATTTCGT CACATGGCCC GAGAGCTGCA TCCGGAGTAC TACAAAGACT GCTGACATCG AGCTTTCTAC AAGGGACTTT CCGCTGGGGA CTTTCCAGGG AGGTGTGGCC TGGGCGGGAC TGGGGAGTGG CGAGCCCTCA GATGCTACAT ATAAGCAGCT GCTTTTTGCC TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC TCAAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGTG GCGCCCGAAC AGGGACTTGA AAGCGAAAGT AAAGCCAGAG GAGATCTCTC GACGCAGGAC TCGGCTTGCT GAAGCGCGCA CGGCAAGAGG CGAGGGGCGG CGACTGGTGA GTACGCCAAA AATTTTGACT AGCGGAGGCT AGAAGGAGAG AGATGGGTGC GAGAGCGTCG GTATTAAGCG GGGGAGAATT AGATAAATGG GAAAAAATTC GGTTAAGGCC AGGGGGAAAG AAACAATATA AACTAAAACA TATAGTATGG GCAAGCAGGG AGCTAGAACG ATTCGCAGTT AATCCTGGCC TTTTAGAGAC ATCAGAAGGC TGTAGACAAA TACTGGGACA GCTACAACCA TCCCTTCAGA CAGGATCAGA AGAACTTAGA TCATTATATA ATACAATAGC AGTCCTCTAT TGTGTGCATC AAAGGATAGA TGTAAAAGAC ACCAAGGAAG CCTTAGATAA GATAGAGGAA GAGCAAAACA AAAGTAAGAA AAAGGCACAG CAAGCAGCAG CTGACACAGG AAACAACAGC CAGGTCAGCC AAAATTACCC TATAGTGCAG AACCTCCAGG GGCAAATGGT ACATCAGGCC ATATCACCTA GAACTTTAAA TGCATGGGTA AAAGTAGTAG AAGAGAAGGC TTTCAGCCCA GAAGTAATAC CCATGTTTTC AGCATTATCA GAAGGAGCCA CCCCACAAGA TTTAAATACC ATGCTAAACA CAGTGGGGGG ACATCAAGCA GCCATGCAAA TGTTAAAAGA GACCATCAAT GAGGAAGCTG CAGAATGGGA TAGATTGCAT CCAGTGCATG CAGGGCCTAT TGCACCAGGC CAGATGAGAG AACCAAGGGG AAGTGACATA GCAGGAACTA CTAGTACCCT TCAGGAACAA ATAGGATGGA TGACACATAA TCCACCTATC CCAGTAGGAG AAATCTATAA AAGATGGATA ATCCTGGGAT TAAATAAAAT AGTAAGAATG TATAGCCCTA CCAGCATTCT GGACATAAGA CAAGGACCAA AGGAACCCTT TAGAGACTAT GTAGACCGAT TCTATAAAAC TCTAAGAGCC GAGCAAGCTT CACAAGAGGT AAAAAATTGG ATGACAGAAA CCTTGTTGGT CCAAAATGCG AACCCAGATT GTAAGACTAT TTTAAAAGCA TTGGGACCAG GAGCGACACT AGAAGAAATG ATGACAGCAT GTCAGGGAGT GGGGGGACCC GGCCATAAAG CAAGAGTTTT GGCTGAAGCA ATGAGCCAAG TAACAAATCC AGCTACCATA ATGATACAGA AAGGCAATTT TAGGAACCAA AGAAAGACTG TTAAGTGTTT CAATTGTGGC AAAGAAGGGC ACATAGCCAA AAATTGCAGG GCCCCTAGGA AAAAGGGCTG TTGGAAATGT GGAAAGGAAG GACACCAAAT GAAAGATTGT ACTGAGAGAC AGGCTAATTT TTTAGGGAAG ATCTGGCCTT CCCACAAGGG AAGGCCAGGG AATTTTCTTC AGAGCAGACC AGAGCCAACA GCCCCACCAG AAGAGAGCTT CAGGTTTGGG GAAGAGACAA CAACTCCCTC TCAGAAGCAG GAGCCGATAG ACAAGGAACT GTATCCTTTA GCTTCCCTCA GATCACTCTT TGGCAGCGAC CCCTCGTCAC AATAAAGATA GGGGGGCAAT TAAAGGAAGC TCTATTAGAT ACAGGAGCAG ATGATACAGT ATTAGAAGAA ATGAATTTGC CAGGAAGATG GAAACCAAAA ATGATAGGGG GAATTGGAGG TTTTATCAAA GTAAGACAGT ATGATCAGAT ACTCATAGAA ATCTGCGGAC ATAAAGCTAT AGGTACAGTA TTAGTAGGAC CTACACCTGT CAACATAATT GGAAGAAATC TGTTGACTCA GATTGGCTGC ACTTTAAATT TTCCCATTAG TCCTATTGAG ACTGTACCAG TAAAATTAAA GCCAGGAATG GATGGCCCAA AAGTTAAACA ATGGCCATTG ACAGAAGAAA AAATAAAAGC ATTAGTAGAA ATTTGTACAG AAATGGAAAA GGAAGGAAAA ATTTCAAAAA TTGGGCCTGA AAATCCATAC AATACTCCAG TATTTGCCAT AAAGAAAAAA GACAGTACTA AATGGAGAAA ATTAGTAGAT TTCAGAGAAC TTAATAAGAG AACTCAAGAT TTCTGGGAAG TTCAATTAGG AATACCACAT CCTGCAGGGT TAAAACAGAA AAAATCAGTA ACAGTACTGG ATGTGGGCGA TGCATATTTT TCAGTTCCCT TAGATAAAGA CTTCAGGAAG TATACTGCAT TTACCATACC TAGTATAAAC AATGAGACAC CAGGGATTAG ATATCAGTAC AATGTGCTTC CACAGGGATG GAAAGGATCA CCAGCAATAT TCCAGTGTAG CATGACAAAA ATCTTAGAGC CTTTTAGAAA ACAAAATCCA GACATAGTCA TCTATCAATA CATGGATGAT TTGTATGTAG GATCTGACTT AGAAATAGGG CAGCATAGAA CAAAAATAGA GGAACTGAGA CAACATCTGT TGAGGTGGGG ATTTACCACA CCAGACAAAA AACATCAGAA AGAACCTCCA TTCCTTTGGA TGGGTTATGA ACTCCATCCT GATAAATGGA CAGTACAGCC TATAGTGCTG CCAGAAAAGG ACAGCTGGAC TGTCAATGAC ATACAGAAAT TAGTGGGAAA ATTGAATTGG GCAAGTCAGA TTTATGCAGG GATTAAAGTA AGGCAATTAT GTAAACTTCT TAGGGGAACC AAAGCACTAA CAGAAGTAGT ACCACTAACA GAAGAAGCAG AGCTAGAACT GGCAGAAAAC AGGGAGATTC TAAAAGAACC GGTACATGGA GTGTATTATG ACCCATCAAA AGACTTAATA GCAGAAATAC AGAAGCAGGG GCAAGGCCAA TGGACATATC AAATTTATCA AGAGCCATTT AAAAATCTGA AAACAGGAAA GTATGCAAGA ATGAAGGGTG CCCACACTAA TGATGTGAAA CAATTAACAG AGGCAGTACA AAAAATAGCC ACAGAAAGCA TAGTAATATG GGGAAAGACT CCTAAATTTA AATTACCCAT ACAAAAGGAA ACATGGGAAG CATGGTGGAC AGAGTATTGG CAAGCCACCT GGATTCCTGA GTGGGAGTTT GTCAATACCC CTCCCTTAGT GAAGTTATGG TACCAGTTAG AGAAAGAACC CATAATAGGA GCAGAAACTT TCTATGTAGA TGGGGCAGCC AATAGGGAAA CTAAATTAGG AAAAGCAGGA TATGTAACTG ACAGAGGAAG ACAAAAAGTT GTCCCCCTAA CGGACACAAC AAATCAGAAG ACTGAGTTAC AAGCAATTCA TCTAGCTTTG CAGGATTCGG GATTAGAAGT AAACATAGTG ACAGACTCAC AATATGCATT GGGAATCATT CAAGCACAAC CAGATAAGAG TGAATCAGAG TTAGTCAGTC AAATAATAGA GCAGTTAATA AAAAAGGAAA AAGTCTACCT GGCATGGGTA CCAGCACACA AAGGAATTGG AGGAAATGAA CAAGTAGATA AATTGGTCAG TGCTGGAATC AGGAAAGTAC TATTTTTAGA TGGAATAGAT AAGGCCCAAG AAGAACATGA GAAATATCAC AGTAATTGGA GAGCAATGGC TAGTGATTTT AACCTACCAC CTGTAGTAGC AAAAGAAATA GTAGCCAGCT GTGATAAATG TCAGCTAAAA GGGGAAGCCA TGCATGGACA AGTAGACTGT AGCCCAGGAA TATGGCAGCT AGATTGTACA CATTTAGAAG GAAAAGTTAT CTTGGTAGCA GTTCATGTAG CCAGTGGATA TATAGAAGCA GAAGTAATTC CAGCAGAGAC AGGGCAAGAA ACAGCATACT TCCTCTTAAA ATTAGCAGGA AGATGGCCAG TAAAAACAGT ACATACAGAC AATGGCAGCA ATTTCACCAG TACTACAGTT AAGGCCGCCT GTTGGTGGGC GGGGATCAAG CAGGAATTTG GCATTCCCTA CAATCCCCAA AGTCAAGGAG TAATAGAATC TATGAATAAA GAATTAAAGA AAATTATAGG ACAGGTAAGA GATCAGGCTG AACATCTTAA GACAGCAGTA CAAATGGCAG TATTCATCCA CAATTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT TCGGGTTTAT TACAGGGACA GCAGAGATCC AGTTTGGAAA GGACCAGCAA AGCTCCTCTG GAAAGGTGAA GGGGCAGTAG TAATACAAGA TAATAGTGAC ATAAAAGTAG TGCCAAGAAG AAAAGCAAAG ATCATCAGGG ATTATGGAAA ACAGATGGCA GGTGATGATT GTGTGGCAAG TAGACAGGAT GAGGATTAAC ACATGGAAAA GATTAGTAAA ACACCATATG TATATTTCAA GGAAAGCTAA GGACTGGTTT TATAGACATC ACTATGAAAG TACTAATCCA AAAATAAGTT CAGAAGTACA CATCCCACTA GGGGATGCTA AATTAGTAAT AACAACATAT TGGGGTCTGC ATACAGGAGA AAGAGACTGG CATTTGGGTC AGGGAGTCTC CATAGAATGG AGGAAAAAGA GATATAGCAC ACAAGTAGAC CCTGACCTAG CAGACCAACT AATTCATCTG CACTATTTTG ATTGTTTTTC AGAATCTGCT ATAAGAAATA CCATATTAGG ACGTATAGTT AGTCCTAGGT GTGAATATCA AGCAGGACAT AACAAGGTAG GATCTCTACA GTACTTGGCA CTAGCAGCAT TAATAAAACC AAAACAGATA AAGCCACCTT TGCCTAGTGT TAGGAAACTG ACAGAGGACA GATGGAACAA GCCCCAGAAG ACCAAGGGCC ACAGAGGGAG CCATACAATG AATGGACACT AGAGCTTTTA GAGGAACTTA AGAGTGAAGC TGTTAGACAT TTTCCTAGGA TATGGCTCCA TAACTTAGGA CAACATATCT ATGAAACTTA CGGGGATACT TGGGCAGGAG TGGAAGCCAT AATAAGAATT CTGCAACAAC TGCTGTTTAT CCATTTCAGA ATTGGGTGTC GACATAGCAG AATAGGCGTT ACTCGACAGA GGAGAGCAAG AAATGGAGCC AGTAGATCCT AGACTAGAGC CCTGGAAGCA TCCAGGAAGT CAGCCTAAAA CTGCTTGTAC CAATTGCTAT TGTAAAAAGT GTTGCTTTCA TTGCCAAGTT TGTTTCATGA CAAAAGCCTT AGGCATCTCC TATGGCAGGA AGAAGCGGAG ACAGCGACGA AGAGCTCATC AGAACAGTCA GACTCATCAA GCTTCTCTAT CAAAGCAGTA AGTAGTACAT GTAATGCAAC CTATAATAGT AGCAATAGTA GCATTAGTAG TAGCAATAAT AATAGCAATA GTTGTGTGGT CCATAGTAAT CATAGAATAT AGGAAAATAT TAAGACAAAG AAAAATAGAC AGGTTAATTG ATAGACTAAT AGAAAGAGCA GAAGACAGTG GCAATGAGAG TGAAGGAGAA GTATCAGCAC TTGTGGAGAT GGGGGTGGAA ATGGGGCACC ATGCTCCTTG GGATATTGAT GATCTGTAGT GCTACAGAAA AATTGTGGGT CACAGTCTAT TATGGGGTAC CTGTGTGGAA GGAAGCAACC ACCACTCTAT TTTGTGCATC AGATGCTAAA GCATATGATA CAGAGGTACA TAATGTTTGG GCCACACATG CCTGTGTACC CACAGACCCC AACCCACAAG AAGTAGTATT GGTAAATGTG ACAGAAAATT TTAACATGTG GAAAAATGAC ATGGTAGAAC AGATGCATGA GGATATAATC AGTTTATGGG ATCAAAGCCT AAAGCCATGT GTAAAATTAA CCCCACTCTG TGTTAGTTTA AAGTGCACTG ATTTGAAGAA TGATACTAAT ACCAATAGTA GTAGCGGGAG AATGATAATG GAGAAAGGAG AGATAAAAAA CTGCTCTTTC AATATCAGCA CAAGCATAAG AGATAAGGTG CAGAAAGAAT ATGCATTCTT TTATAAACTT GATATAGTAC CAATAGATAA TACCAGCTAT AGGTTGATAA GTTGTAACAC CTCAGTCATT ACACAGGCCT GTCCAAAGGT ATCCTTTGAG CCAATTCCCA TACATTATTG TGCCCCGGCT GGTTTTGCGA TTCTAAAATG TAATAATAAG ACGTTCAATG GAACAGGACC ATGTACAAAT GTCAGCACAG TACAATGTAC ACATGGAATC AGGCCAGTAG TATCAACTCA ACTGCTGTTA AATGGCAGTC TAGCAGAAGA AGATGTAGTA ATTAGATCTG CCAATTTCAC AGACAATGCT AAAACCATAA TAGTACAGCT GAACACATCT GTAGAAATTA ATTGTACAAG ACCCAACAAC AATACAAGAA AAAGTATCCG TATCCAGAGG GGACCAGGGA GAGCATTTGT TACAATAGGA AAAATAGGAA ATATGAGACA AGCACATTGT AACATTAGTA GAGCAAAATG GAATGCCACT TTAAAACAGA TAGCTAGCAA ATTAAGAGAA CAATTTGGAA ATAATAAAAC AATAATCTTT AAGCAATCCT CAGGAGGGGA CCCAGAAATT GTAACGCACA GTTTTAATTG TGGAGGGGAA TTTTTCTACT GTAATTCAAC ACAACTGTTT AATAGTACTT GGTTTAATAG TACTTGGAGT ACTGAAGGGT CAAATAACAC TGAAGGAAGT GACACAATCA CACTCCCATG CAGAATAAAA CAATTTATAA ACATGTGGCA GGAAGTAGGA AAAGCAATGT ATGCCCCTCC CATCAGTGGA CAAATTAGAT GTTCATCAAA TATTACTGGG CTGCTATTAA CAAGAGATGG TGGTAATAAC AACAATGGGT CCGAGATCTT CAGACCTGGA GGAGGCGATA TGAGGGACAA TTGGAGAAGT GAATTATATA AATATAAAGT AGTAAAAATT GAACCATTAG GAGTAGCACC CACCAAGGCA AAGAGAAGAG TGGTGCAGAG AGAAAAAAGA GCAGTGGGAA TAGGAGCTTT GTTCCTTGGG TTCTTGGGAG CAGCAGGAAG CACTATGGGC GCAGCGTCAA TGACGCTGAC GGTACAGGCC AGACAATTAT TGTCTGATAT AGTGCAGCAG CAGAACAATT TGCTGAGGGC TATTGAGGCG CAACAGCATC TGTTGCAACT CACAGTCTGG GGCATCAAAC AGCTCCAGGC AAGAATCCTG GCTGTGGAAA GATACCTAAA GGATCAACAG CTCCTGGGGA TTTGGGGTTG CTCTGGAAAA CTCATTTGCA CCACTGCTGT GCCTTGGAAT GCTAGTTGGA GTAATAAATC TCTGGAACAG ATTTGGAATA ACATGACCTG GATGGAGTGG GACAGAGAAA TTAACAATTA CACAAGCTTA ATACACTCCT TAATTGAAGA ATCGCAAAAC CAGCAAGAAA AGAATGAACA AGAATTATTG GAATTAGATA AATGGGCAAG TTTGTGGAAT TGGTTTAACA TAACAAATTG GCTGTGGTAT ATAAAATTAT TCATAATGAT AGTAGGAGGC TTGGTAGGTT TAAGAATAGT TTTTGCTGTA CTTTCTATAG TGAATAGAGT TAGGCAGGGA TATTCACCAT TATCGTTTCA GACCCACCTC CCAATCCCGA GGGGACCCGA CAGGCCCGAA GGAATAGAAG AAGAAGGTGG AGAGAGAGAC AGAGACAGAT CCATTCGATT AGTGAACGGA TCCTTAGCAC TTATCTGGGA CGATCTGCGG AGCCTGTGCC TCTTCAGCTA CCACCGCTTG AGAGACTTAC TCTTGATTGT AACGAGGATT GTGGAACTTC TGGGACGCAG GGGGTGGGAA GCCCTCAAAT ATTGGTGGAA TCTCCTACAA TATTGGAGTC AGGAGCTAAA GAATACCCAT ACGACGTACC AGATTACGCT TAGCTCGAGA CCTAGAAAAA CATGGAGCAA TCACAAGTAG CAATACAGCA GCTAACAATG CTGCTTGTGC CTGGCTAGAA GCACAAGAGG AGGAAGAGGT GGGTTTTCCA GTCACACCTC AGGTACCTTT AAGACCAATG ACTTACAAGG CAGCTGTAGA TCTTAGCCAC TTTTTAAAAG AAAAGGGGGG ACTGGAAGGG CTAATTCACT CCCAAAGAAG ACAAGATATC CTTGATCTGT GGATCTACCA CACACAAGGC TACTTCCCTG ATTGGCAGAA CTACACACCA GGGCCAGGGG TCAGATATCC ACTGACCTTT GGATGGTGCT ACAAGCTAGT ACCAGTTGAG CCAGATAAGG TAGAAGAGGC CAATAAAGGA GAGAACACCA GCTTGTTACA CCCTGTGAGC CTGCATGGAA TGGATGACCC TGAGAGAGAA GTGTTAGAGT GGAGGTTTGA CAGCCGCCTA GCATTTCATC ACGTGGCCCG AGAGCTGCAT CCGGAGTACT TCAAGAACTG CTGACATCGA GCTTGCTACA AGGGACTTTC CGCTGGGGAC TTTCCAGGGA GGCGTGGCCT GGGCGGGACT GGGGAGTGGC GAGCCCTCAG ATGCTGCATA TAAGCAGCTG CTTTTTGCCT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT TTAGTCAGTG TGGAAAATCT CTAGCACCCA GGAGGTAGAG GTTGCAGTGA GCCAAGATCG CGCCACTGCA TTCCAGCCTG GGCAAGAAAA CAAGACTGTC TAAAATAATA ATAATAAGTT AAGGGTATTA AATATATTTA TACATGGAGG TCATAAAAAT ATATATATTT GGGCTGGGCG CAGTGGCTCA CACCTGCGCC CGGCCCTTTG GGAGGCCGAG GCAGGTGGAT CACCTGAGTT TGGGAGTTCC AGACCAGCCT GACCAACATG GAGAAACCCC TTCTCTGTGT ATTTTTAGTA GATTTTATTT TATGTGTATT TTATTCACAG GTATTTCTGG AAAACTGAAA CTGTTTTTCC TCTACTCTGA TACCACAAGA ATCATCAGCA CAGAGGAAGA CTTCTGTGAT CAAATGTGGT GGGAGAGGGA GGTTTTCACC AGCACATGAG CAGTCAGTTC TGCCGCAGAC TCGGCGGGTG TCCTTCGGTT CAGTTCCAAC ACCGCCTGCC TGGAGAGAGG TCAGACCACA GGGTGAGGGC TCAGTCCCCA AGACATAAAC ACCCAAGACA TAAACACCCA ACAGGTCCAC CCCGCCTGCT GCCCAGGCAG AGCCGATTCA CCAAGACGGG AATTAGGATA GAGAAAGAGT AAGTCACACA GAGCCGGCTG TGCGGGAGAA CGGAGTTCTA TTATGACTCA AATCAGTCTC CCCAAGCATT CGGGGATCAG AGTTTTTAAG GATAACTTAG TGTGTAGGGG GCCAGTGAGT TGGAGATGAA AGCGTAGGGA GTCGAAGGTG TCCTTTTGCG CCGAGTCAGT TCCTGGGTGG GGGCCACAAG ATCGGATGAG CCAGTTTATC AATCCGGGGG TGCCAGCTGA TCCATGGAGT GCAGGGTCTG CAAAATATCT CAAGCACTGA TTGATCTTAG GTTTTACAAT AGTGATGTTA CCCCAGGAAC AATTTGGGGA AGGTCAGAAT CTTGTAGCCT GTAGCTGCAT GACTCCTAAA CCATAATTTC TTTTTTGTTT TTTTTTTTTT ATTTTTGAGA CAGGGTCTCA CTCTGTCACC TAGGCTGGAG TGCAGTGGTG CAATCACAGC TCACTGCAGC CTCAACGTCG TAAGCTCAAG CGATCCTCCC ACCTCAGCCT GCCTGGTAGC TGAGACTACA AGCGACGCCC CAGTTAATTT TTGTATTTTT GGTAGAGGCA GCGTTTTGCC GTGTGGCCCT GGCTGGTCTC GAACTCCTGG GCTCAAGTGA TCCAGCCTCA GCCTCCCAAA GTGCTGGGAC AACCGGGGCC AGTCACTGCA CCTGGCCCTA AACCATAATT TCTAATCTTT TGGCTAATTT GTTAGTCCTA CAAAGGCAGT CTAGTCCCCA GGCAAAAAGG GGGTTTGTTT CGGGAAAGGG CTGTTACTGT CTTTGTTTCA AACTATAAAC TAAGTTCCTC CTAAACTTAG TTCGGCCTAC ACCCAGGAAT GAACAAGGAG AGCTTGGAGG TTAGAAGCAC GATGGAATTG GTTAGGTCAG ATCTCTTTCA CTGTCTGAGT TATAATTTTG CAATGGTGGT TCAAAGACTG CCCGCTTCTG ACACCAGTCG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGGGGAGG CAGAGATTGC AGTAAGCTGA GATCGCAGCA CTGCACTCCA GCCTGGGCGA CAGAGTAAGA CTCTGTCTCA AAAATAAAAT AAATAAATCA ATCAGATATT CCAATCTTTT CCTTTATTTA TTTATTTATT TTCTATTTTG GAAACACAGT CCTTCCTTAT TCCAGAATTA CACATATATT CTATTTTTCT TTATATGCTC CAGTTTTTTT TAGACCTTCA CCTGAAATGT GTGTATACAA AATCTAGGCC AGTCCAGCA
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