pCMV-AEBP1(human)-3×FLAG-Neo
$99.00
In stock
SKU
PL38566
NM_001129.5;ACLP
100μg liquid plasmid(Instantly):$120
| name | pCMV-AEBP1(human)-3×FLAG-Neo | another name | apCMV-AEBP1(human)-3×FLAG-Neo |
| Insert Size (bp) | 3474bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCATGGC GGCCGTGCGC GGGGCGCCCC TGCTCAGCTG CCTCCTGGCG TTGCTGGCCC TGTGCCCTGG AGGGCGCCCG CAGACGGTGC TGACCGACGA CGAGATCGAG GAGTTCCTCG AGGGCTTCCT GTCAGAGCTA GAACCTGAGC CCCGGGAGGA CGACGTGGAG GCCCCGCCGC CTCCCGAGCC CACCCCGCGG GTCCGAAAAG CCCAGGCGGG GGGCAAGCCA GGGAAGCGGC CAGGGACGGC CGCAGAAGTG CCTCCGGAAA AGACCAAAGA CAAAGGGAAG AAAGGCAAGA AAGACAAAGG CCCCAAGGTG CCCAAGGAGT CCTTGGAGGG GTCCCCCAGG CCGCCCAAGA AGGGGAAGGA GAAGCCACCC AAGGCCACCA AGAAGCCCAA GGAGAAGCCA CCTAAGGCCA CCAAGAAGCC CAAGGAGAAG CCACCCAAGG CCACCAAGAA GCCCAAAGAG AAGCCACCCA AGGCCACCAA GAAGCCCCCG TCAGGGAAGA GGCCCCCCAT TCTGGCTCCC TCAGAAACCC TGGAGTGGCC ACTGCCCCCA CCCCCCAGCC CTGGCCCCGA GGAGCTACCC CAGGAGGGAG GGGCGCCCCT CTCAAATAAC TGGCAGAATC CAGGAGAGGA GACCCATGTG GAGGCACGGG AGCACCAGCC TGAGCCGGAG GAGGAGACCG AGCAACCCAC ACTGGACTAC AATGACCAGA TCGAGAGGGA GGACTATGAG GACTTTGAGT ACATTCGGCG CCAGAAGCAA CCCAGGCCAC CCCCAAGCAG AAGGAGGAGG CCCGAGCGGG TCTGGCCAGA GCCCCCTGAG GAGAAGGCCC CGGCCCCAGC CCCGGAGGAG AGGATTGAGC CTCCTGTGAA GCCTCTGCTG CCCCCGCTGC CCCCTGACTA TGGTGATGGT TACGTGATCC CCAACTACGA TGACATGGAC TATTACTTTG GGCCTCCTCC GCCCCAGAAG CCCGATGCTG AGCGCCAGAC AGACGAAGAG AAGGAGGAGC TGAAGAAACC CAAAAAGGAG GACAGCAGCC CCAAGGAGGA GACCGACAAG TGGGCAGTGG AGAAGGGCAA GGACCACAAA GAGCCCCGAA AGGGCGAGGA GTTGGAGGAG GAGTGGACGC CTACGGAGAA AGTCAAGTGT CCCCCCATTG GGATGGAGTC ACACCGTATT GAGGACAACC AGATCCGAGC CTCCTCCATG CTGCGCCACG GCCTGGGGGC ACAGCGCGGC CGGCTCAACA TGCAGACCGG TGCCACTGAG GACGACTACT ATGATGGTGC GTGGTGTGCC GAGGACGATG CCAGGACCCA GTGGATAGAG GTGGACACCA GGAGGACTAC CCGGTTCACA GGCGTCATCA CCCAGGGCAG AGACTCCAGC ATCCATGACG ATTTTGTGAC CACCTTCTTC GTGGGCTTCA GCAATGACAG CCAGACATGG GTGATGTACA CCAACGGCTA TGAGGAAATG ACCTTTCATG GGAACGTGGA CAAGGACACA CCCGTGCTGA GTGAGCTCCC AGAGCCGGTG GTGGCTCGTT TCATCCGCAT CTACCCACTC ACCTGGAATG GCAGCCTGTG CATGCGCCTG GAGGTGCTGG GGTGCTCTGT GGCCCCTGTC TACAGCTACT ACGCACAGAA TGAGGTGGTG GCCACCGATG ACCTGGATTT CCGGCACCAC AGCTACAAGG ACATGCGCCA GCTCATGAAG GTGGTGAACG AGGAGTGCCC CACCATCACC CGCACTTACA GCCTGGGCAA GAGCTCACGA GGCCTCAAGA TCTATGCCAT GGAGATCTCA GACAACCCTG GGGAGCATGA ACTGGGGGAG CCCGAGTTCC GCTACACTGC TGGGATCCAT GGCAACGAGG TGCTGGGCCG AGAGCTGTTG CTGCTGCTCA TGCAGTACCT GTGCCGAGAG TACCGCGATG GGAACCCACG TGTGCGCAGC CTGGTGCAGG ACACACGCAT CCACCTGGTG CCCTCACTGA ACCCTGATGG CTACGAGGTG GCAGCGCAGA TGGGCTCAGA GTTTGGGAAC TGGGCGCTGG GACTGTGGAC TGAGGAGGGC TTTGACATCT TTGAAGATTT CCCGGATCTC AACTCTGTGC TCTGGGGAGC TGAGGAGAGG AAATGGGTCC CCTACCGGGT CCCCAACAAT AACTTGCCCA TCCCTGAACG CTACCTTTCG CCAGATGCCA CGGTATCCAC GGAGGTCCGG GCCATCATTG CCTGGATGGA GAAGAACCCC TTCGTGCTGG GAGCAAATCT GAACGGCGGC GAGCGGCTAG TATCCTACCC CTACGATATG GCCCGCACGC CTACCCAGGA GCAGCTGCTG GCCGCAGCCA TGGCAGCAGC CCGGGGGGAG GATGAGGACG AGGTCTCCGA GGCCCAGGAG ACTCCAGACC ACGCCATCTT CCGGTGGCTT GCCATCTCCT TCGCCTCCGC ACACCTCACC TTGACCGAGC CCTACCGCGG AGGCTGCCAA GCCCAGGACT ACACCGGCGG CATGGGCATC GTCAACGGGG CCAAGTGGAA CCCCCGGACC GGGACTATCA ATGACTTCAG TTACCTGCAT ACCAACTGCC TGGAGCTCTC CTTCTACCTG GGCTGTGACA AGTTCCCTCA TGAGAGTGAG CTGCCCCGCG AGTGGGAGAA CAACAAGGAG GCGCTGCTCA CCTTCATGGA GCAGGTGCAC CGCGGCATTA AGGGGGTGGT GACGGACGAG CAAGGCATCC CCATTGCCAA CGCCACCATC TCTGTGAGTG GCATTAATCA CGGCGTGAAG ACAGCCAGTG GTGGTGATTA CTGGCGAATC TTGAACCCGG GTGAGTACCG CGTGACAGCC CACGCGGAGG GCTACACCCC GAGCGCCAAG ACCTGCAATG TTGACTATGA CATCGGGGCC ACTCAGTGCA ACTTCATCCT GGCTCGCTCC AACTGGAAGC GCATCCGGGA GATCATGGCC ATGAACGGGA ACCGGCCTAT CCCACACATA GACCCATCGC GCCCTATGAC CCCCCAACAG CGACGCCTGC AGCAGCGACG CCTACAACAC CGCCTGCGGC TTCGGGCACA GATGCGGCTG CGGCGCCTCA ACGCCACCAC CACCCTAGGC CCCCACACTG TGCCTCCCAC GCTGCCCCCT GCCCCTGCCA CCACCCTGAG CACTACCATA GAGCCCTGGG GCCTCATACC GCCAACCACC GCTGGCTGGG AGGAGTCGGA GACTGAGACC TACACAGAGG TGGTGACAGA GTTTGGGACC GAGGTGGAGC CCGAGTTTGG GACCAAGGTG GAGCCCGAGT TTGAGACCCA GTTGGAGCCT GAGTTTGAGA CCCAGCTGGA ACCCGAGTTT GAGGAAGAGG AGGAGGAGGA GAAAGAGGAG GAGATAGCCA CTGGCCAGGC ATTCCCCTTC ACAACAGTAG AGACCTACAC AGTGAACTTT GGGGACTTCG GATCCGACTA CAAAGACCAT GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA GTAGTGAGGG CCCGATATCT CGACAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAG CTGACGTCCT TTCCATGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCCTGGAAA CGGGGGAGGC TAACTGAAAC ACGGAAGGAG ACAATACCGG AAGGAACCCG CGCTATGACG GCAATAAAAA GACAGAATAA AACGCACGGG TGTTGGGTCG TTTGTTCATA AACGCGGGGT TCGGTCCCAG GGCTGGCACT CTGTCGATAC CCCACCGAGA CCCCATTGGG GCCAATACGC CCGCGTTTCT TCCTTTTCCC CACCCCACCC CCCAAGTTCG GGTGAAGGCC CAGGGCTCGC AGCCAACGTC GGGGCGGCAG GCCCTGCCAT AGCAGATCTG CGCAGCTGGG GCTCTAGGGG GTATCCCCAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGCATCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGG GGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCAATGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCGACGGA TCGGGAGATC TCCCGATCCC CTATGGTCGA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATCTGCTC CCTGCTTGTG TGTTGGAGGT CGCTGAGTAG TGCGCGAGCA AAATTTAAGC TACAACAAGG CAAGGCTTGA CCGACAATTG CATGAAGAAT CTGCTTAGG
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