pCasKP-apr②
$84.00
In stock
SKU
PL38553
100μg liquid plasmid(Instantly):$127
| name | pCasKP-apr② | another name | apCasKP-apr② |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | CRISPR,Red |
| Fragment species | Prokaryotic resistance | Apr | |
| Selective marker | Fluorescent labeling | ||
| Promoter | araBAD | Replicon | pSC101 |
| Competence | DH5a | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | Low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG ATCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG CTAGCGAAAA GATGTTTCGT GAAGCCGTCG ACGCTTATAA AAAATGGATA TTAATACTGA AACTGAGATC AAGCAAAAGC ATTCACTAAC CCCCTTTCCT GTTTTCCTAA TCAGCCCGGC ATTTCGCGGG CGATATTTTC ACAGCTATTT CAGGAGTTCA GCCATGAACG CTTATTACAT TCAGGATCGT CTTGAGGCTC AGAGCTGGGC GCGTCACTAC CAGCAGCTCG CCCGTGAAGA GAAAGAGGCA GAACTGGCAG ACGACATGGA AAAAGGCCTG CCCCAGCACC TGTTTGAATC GCTATGCATC GATCATTTGC AACGCCACGG GGCCAGCAAA AAATCCATTA CCCGTGCGTT TGATGACGAT GTTGAGTTTC AGGAGCGCAT GGCAGAACAC ATCCGGTACA TGGTTGAAAC CATTGCTCAC CACCAGGTTG ATATTGATTC AGAGGTATAA AACGAATGAG TACTGCACTC GCAACGCTGG CTGGGAAGCT GGCTGAACGT GTCGGCATGG ATTCTGTCGA CCCACAGGAA CTGATCACCA CTCTTCGCCA GACGGCATTT AAAGGTGATG CCAGCGATGC GCAGTTCATC GCATTACTGA TCGTTGCCAA CCAGTACGGC CTTAATCCGT GGACGAAAGA AATTTACGCC TTTCCTGATA AGCAGAATGG CATCGTTCCG GTGGTGGGCG TTGATGGCTG GTCCCGCATC ATCAATGAAA ACCAGCAGTT TGATGGCATG GACTTTGAGC AGGACAATGA ATCCTGTACA TGCCGGATTT ACCGCAAGGA CCGTAATCAT CCGATCTGCG TTACCGAATG GATGGATGAA TGCCGCCGCG AACCATTCAA AACTCGCGAA GGCAGAGAAA TCACGGGGCC GTGGCAGTCG CATCCCAAAC GGATGTTACG TCATAAAGCC ATGATTCAGT GTGCCCGTCT GGCCTTCGGA TTTGCTGGTA TCTATGACAA GGATGAAGCC GAGCGCATTG TCGAAAATAC TGCATACACT GCAGAACGTC AGCCGGAACG CGACATCACT CCGGTTAACG ATGAAACCAT GCAGGAGATT AACACTCTGC TGATCGCCCT GGATAAAACA TGGGATGACG ACTTATTGCC GCTCTGTTCC CAGATATTTC GCCGCGACAT TCGTGCATCG TCAGAACTGA CACAGGCCGA AGCAGTAAAA GCTCTTGGAT TCCTGAAACA GAAAGCCGCA GAGCAGAAGG TGGCAGCATG ACACCGGACA TTATCCTGCA GCGTACCGGG ATCGATGTGA GAGCTGTCGA ACAGGGGGAA GATGCGTGGC ACAAATTACG GCTCGGCGTC ATCACCGCTT CAGAAGTTCA CAACGTGATA GCAAAACCCC GCTCCGGAAA GAAGTGGCCT GACATGAAAA TGTCCTACTT CCACACCCTG CTTGCTGAGG TTTGCACCGG TGTGGCTCCG GAAGTTAACG CTAAAGCACT GGCCTGGGGA AAACAGTACG AGAACGACGC CAGAACCCTG TTTGAATTCA CTTCCGGCGT GAATGTTACT GAATCCCCGA TCATCTATCG CGACGAAAGT ATGCGTACCG CCTGCTCTCC CGATGGTTTA TGCAGTGACG GCAACGGCCT TGAACTGAAA TGCCCGTTTA CCTCCCGGGA TTTCATGAAG TTCCGGCTCG GTGGTTTCGA GGCCATAAAG TCAGCTTACA TGGCCCAGGT GCAGTACAGC ATGTGGGTGA CGCGAAAAAA TGCCTGGTAC TTTGCCAACT ATGACCCGCG TATGAAGCGT GAAGGCCTGC ATTATGTCGT GATTGAGCGG GATGAAAAGT ACATGGCGAG TTTTGACGAG ATCGTGCCGG AGTTCATCGA AAAAATGGAC GAGGCACTGG CTGAAATTGG TTTTGTATTT GGGGAGCAAT GGCGATGACG CATCCTCACG ATAATAAGCT TCCTGCTGAA CATCAAAGGC AAGAAAACAT CTGTTGTCAA AGACAGCATC CTTGAACAAG GACAATTAAC AGTTAACAAA TAAAAACGCA AAAGAAAATG CCGATATTGA CTACCGGAAG CAGTGTGACC GTGTGCTTCT CAAATGCCTG ATTCAGGCTG TCTATGTGTG ACTGTTGAGC TGTAACAAGT TGTCTCAGGT GTTCAATTTC ATGTTCTAGT TGCTTTGTTT TACTGGTTTC ACCTGTTCTA TTAGGTGTTA CATGCTGTTC ATCTGTTACA TTGTCGATCT GTTCATGGTG AACAGCTTTA AATGCACCAA AAACTCGTAA AAGCTCTGAT GTATCTATCT TTTTTACACC GTTTTCATCT GTGCATATAG CTATACTGAT TTCGTCAGAC TCACAGTCAA ACATGCCGGT CAGTTGGCCT GGTGATGGCG GGATCGTTGT ATATTTCTTG ACACCTTTTC GGCACCGCCC TAAAATTCTG CGTCCTCATA ATATATGAGG CGATTTATTA CGTGTTTACG AAGCAAAAGC TAAAACCAGG AGCTATTTAA TGGATAAGAA ATACTCAATA GGCTTAGATA TCGGCACAAA TAGCGTCGGA TGGGCGGTGA TCACTGATGA ATATAAGGTT CCGTCTAAAA AGTTCAAGGT TCTGGGAAAT ACAGACCGCC ACAGTATCAA AAAAAATCTT ATAGGGGCTC TTTTATTTGA CAGTGGAGAG ACAGCGGAAG CGACTCGTCT CAAACGGACA GCTCGTAGAA GGTATACACG TCGGAAGAAT CGTATTTGTT ATCTACAGGA GATTTTTTCA AATGAGATGG CGAAAGTAGA TGATAGTTTC TTTCATCGAC TTGAAGAGTC TTTTTTGGTG GAAGAAGACA AGAAGCATGA ACGTCATCCT ATTTTTGGAA ATATAGTAGA TGAAGTTGCT TATCATGAGA AATATCCAAC TATCTATCAT CTGCGAAAAA AATTGGTAGA TTCTACTGAT AAAGCGGATT TGCGCTTAAT CTATTTGGCC TTAGCGCATA TGATTAAGTT TCGTGGTCAT TTTTTGATTG AGGGAGATTT AAATCCTGAT AATAGTGATG TGGACAAACT ATTTATCCAG TTGGTACAAA CCTACAATCA ATTATTTGAA GAAAACCCTA TTAACGCAAG TGGAGTAGAT GCTAAAGCGA TTCTTTCTGC ACGATTGAGT AAATCAAGAC GATTAGAAAA TCTCATTGCT CAGCTCCCCG GTGAGAAGAA AAATGGCTTA TTTGGGAATC TCATTGCTTT GTCATTGGGT TTGACCCCTA ATTTTAAATC AAATTTTGAT TTGGCAGAAG ATGCTAAATT ACAGCTTTCA AAAGATACTT ACGATGATGA TTTAGATAAT TTATTGGCGC AAATTGGAGA TCAATATGCT GATTTGTTTT TGGCAGCTAA GAATTTATCA GATGCTATTT TACTTTCAGA TATCCTAAGA GTAAATACTG AAATAACTAA GGCTCCCCTA TCAGCTTCAA TGATTAAACG CTACGATGAA CATCATCAAG ACTTGACTCT TTTAAAAGCT TTAGTTCGAC AACAACTTCC AGAAAAGTAT AAAGAAATCT TTTTTGATCA ATCAAAAAAC GGATATGCAG GTTATATTGA TGGGGGAGCT AGCCAAGAAG AATTTTATAA ATTTATCAAA CCAATTTTAG AAAAAATGGA TGGTACTGAG GAATTATTGG TGAAACTAAA TCGTGAAGAT TTGCTGCGCA AGCAACGGAC CTTTGACAAC GGCTCTATTC CCCATCAAAT TCACTTGGGT GAGCTGCATG CTATTTTGAG AAGACAAGAA GACTTTTATC CATTTTTAAA AGACAATCGT GAGAAGATTG AAAAAATCTT GACTTTTCGA ATTCCTTATT ATGTTGGTCC ATTGGCGCGT GGCAATAGTC GTTTTGCATG GATGACTCGG AAGTCTGAAG AAACAATTAC CCCATGGAAT TTTGAAGAAG TTGTCGATAA AGGTGCTTCA GCTCAATCAT TTATTGAACG CATGACAAAC TTTGATAAAA ATCTTCCAAA TGAAAAAGTA CTACCAAAAC ATAGTTTGCT TTATGAGTAT TTTACGGTTT ATAACGAATT GACAAAGGTC AAATATGTTA CTGAAGGAAT GCGAAAACCA GCATTTCTTT CAGGTGAACA GAAGAAAGCC ATTGTTGATT TACTCTTCAA AACAAATCGA AAAGTAACCG TTAAGCAATT AAAAGAAGAT TATTTCAAAA AAATAGAATG TTTTGATAGT GTTGAAATTT CAGGAGTTGA AGATAGATTT AATGCTTCAT TAGGTACCTA CCATGATTTG CTAAAAATTA TTAAAGATAA AGATTTTTTG GATAATGAAG AAAATGAAGA TATCTTAGAG GATATTGTTT TAACATTGAC CTTATTTGAA GATAGGGAGA TGATTGAGGA AAGACTTAAA ACATATGCTC ACCTCTTTGA TGATAAGGTG ATGAAACAGC TTAAACGTCG CCGTTATACT GGTTGGGGAC GTTTGTCTCG AAAATTGATT AATGGTATTA GGGATAAGCA ATCTGGCAAA ACAATATTAG ATTTTTTGAA ATCAGATGGT TTTGCCAATC GCAATTTTAT GCAGCTGATC CATGATGATA GTTTGACATT TAAAGAAGAC ATTCAAAAAG CACAAGTGTC TGGACAAGGC GATAGTTTAC ATGAACATAT TGCAAATTTA GCTGGTAGCC CTGCTATTAA AAAAGGTATT TTACAGACTG TAAAAGTTGT TGATGAATTG GTCAAAGTAA TGGGGCGGCA TAAGCCAGAA AATATCGTTA TTGAAATGGC ACGTGAAAAT CAGACAACTC AAAAGGGCCA GAAAAATTCG CGAGAGCGTA TGAAACGAAT CGAAGAAGGT ATCAAAGAAT TAGGAAGTCA GATTCTTAAA GAGCATCCTG TTGAAAATAC TCAATTGCAA AATGAAAAGC TCTATCTCTA TTATCTCCAA AATGGAAGAG ACATGTATGT GGACCAAGAA TTAGATATTA ATCGTTTAAG TGATTATGAT GTCGATCACA TTGTTCCACA AAGTTTCCTT AAAGACGATT CAATAGACAA TAAGGTCTTA ACGCGTTCTG ATAAAAATCG TGGTAAATCG GATAACGTTC CAAGTGAAGA AGTAGTCAAA AAGATGAAAA ACTATTGGAG ACAACTTCTA AACGCCAAGT TAATCACTCA ACGTAAGTTT GATAATTTAA CGAAAGCTGA ACGTGGAGGT TTGAGTGAAC TTGATAAAGC TGGTTTTATC AAACGCCAAT TGGTTGAAAC TCGCCAAATC ACTAAGCATG TGGCACAAAT TTTGGATAGT CGCATGAATA CTAAATACGA TGAAAATGAT AAACTTATTC GAGAGGTTAA AGTGATTACC TTAAAATCTA AATTAGTTTC TGACTTCCGA AAAGATTTCC AATTCTATAA AGTACGTGAG ATTAACAATT ACCATCATGC CCATGATGCG TATCTAAATG CCGTCGTTGG AACTGCTTTG ATTAAGAAAT ATCCAAAACT TGAATCGGAG TTTGTCTATG GTGATTATAA AGTTTATGAT GTTCGTAAAA TGATTGCTAA GTCTGAGCAA GAAATAGGCA AAGCAACCGC AAAATATTTC TTTTACTCTA ATATCATGAA CTTCTTCAAA ACAGAAATTA CACTTGCAAA TGGAGAGATT CGCAAACGCC CTCTAATCGA AACTAATGGG GAAACTGGAG AAATTGTCTG GGATAAAGGG CGAGATTTTG CCACAGTGCG CAAAGTATTG TCCATGCCCC AAGTCAATAT TGTCAAGAAA ACAGAAGTAC AGACAGGCGG ATTCTCCAAG GAGTCAATTT TACCAAAAAG AAATTCGGAC AAGCTTATTG CTCGTAAAAA AGACTGGGAT CCAAAAAAAT ATGGTGGTTT TGATAGTCCA ACGGTAGCTT ATTCAGTCCT AGTGGTTGCT AAGGTGGAAA AAGGGAAATC GAAGAAGTTA AAATCCGTTA AAGAGTTACT AGGGATCACA ATTATGGAAA GAAGTTCCTT TGAAAAAAAT CCGATTGACT TTTTAGAAGC TAAAGGATAT AAGGAAGTTA AAAAAGACTT AATCATTAAA CTACCTAAAT ATAGTCTTTT TGAGTTAGAA AACGGTCGTA AACGGATGCT GGCTAGTGCC GGAGAATTAC AAAAAGGAAA TGAGCTGGCT CTGCCAAGCA AATATGTGAA TTTTTTATAT TTAGCTAGTC ATTATGAAAA GTTGAAGGGT AGTCCAGAAG ATAACGAACA AAAACAATTG TTTGTGGAGC AGCATAAGCA TTATTTAGAT GAGATTATTG AGCAAATCAG TGAATTTTCT AAGCGTGTTA TTTTAGCAGA TGCCAATTTA GATAAAGTTC TTAGTGCATA TAACAAACAT AGAGACAAAC CAATACGTGA ACAAGCAGAA AATATTATTC ATTTATTTAC GTTGACGAAT CTTGGAGCTC CCGCTGCTTT TAAATATTTT GATACAACAA TTGATCGTAA ACGATATACG TCTACAAAAG AAGTTTTAGA TGCCACTCTT ATCCATCAAT CCATCACTGG TCTTTATGAA ACACGCATTG ATTTGAGTCA GCTAGGAGGT GACTGATGGC TGGTTGGCGT ACTGTTGTGG TAAATACCCA TATGGACAGT TTTCCCTTTG ATATGTAACG GTGAACAGTT GTTCTACTTT TGTTTGTTAG TCTTGATGCT TCACTGATAG ATACAAGAGC CATAAGAACC TCAGATCCTT CCGTATTTAG CCAGTATGTT CTCTAGTGTG GTTCGTTGTT TTTGCGTGAG CCATGAGAAC GAACCATTGA GATCATACTT ACTTTGCATG TCACTCAAAA ATTTTGCCTC AAAACTGGTG AGCTGAATTT TTGCAGTTAA AGCATCGTGT AGTGTTTTTC TTAGTCCGTT ATGTAGGTAG GAATCTGATG TAATGGTTGT TGGTATTTTG TCACCATTCA TTTTTATCTG GTTGTTCTCA AGTTCGGTTA CGAGATCCAT TTGTCTATCT AGTTCAACTT GGAAAATCAA CGTATCAGTC GGGCGGCCTC GCTTATCAAC CACCAATTTC ATATTGCTGT AAGTGTTTAA ATCTTTACTT ATTGGTTTCA AAACCCATTG GTTAAGCCTT TTAAACTCAT GGTAGTTATT TTCAAGCATT AACATGAACT TAAATTCATC AAGGCTAATC TCTATATTTG CCTTGTGAGT TTTCTTTTGT GTTAGTTCTT TTAATAACCA CTCATAAATC CTCATAGAGT ATTTGTTTTC AAAAGACTTA ACATGTTCCA GATTATATTT TATGAATTTT TTTAACTGGA AAAGATAAGG CAATATCTCT TCACTAAAAA CTAATTCTAA TTTTTCGCTT GAGAACTTGG CATAGTTTGT CCACTGGAAA ATCTCAAAGC CTTTAACCAA AGGATTCCTG ATTTCCACAG TTCTCGTCAT CAGCTCTCTG GTTGCTTTAG CTAATACACC ATAAGCATTT TCCCTACTGA TGTTCATCAT CTGAACGTAT TGGTTATAAG TGAACGATAC CGTCCGTTCT TTCCTTGTAG GGTTTTCAAT CGTGGGGTTG AGTAGTGCCA CACAGCATAA AATTAGCTTG GTTTCATGCT CCGTTAAGTC ATAGCGACTA ATCGCTAGTT CATTTGCTTT GAAAACAACT AATTCAGACA TACATCTCAA TTGGTCTAGG TGATTTTAAT CACTATACCA ATTGAGATGG GCTAGTCAAT GATAATTACT AGTCCTTTTC CTTTGAGTTG TGGGTATCTG TAAATTCTGC TAGACCTTTG CTGGAAAACT TGTAAATTCT GCTAGACCCT CTGTAAATTC CGCTAGACCT TTGTGTGTTT TTTTTGTTTA TATTCAAGTG GTTATAATTT ATAGAATAAA GAAAGAATAA AAAAAGATAA AAAGAATAGA TCCCAGCCCT GTGTATAACT CACTACTTTA GTCAGTTCCG CAGTATTACA AAAGGATGTC GCAAACGCTG TTTGCTCCTC TACAAAACAG ACCTTAAAAC CCTAAAGGCT TAAGTAGCAC CCTCGCAAGC TCGGGCAAAT CGCTGAATAT TCCTTTTGTC TCCGACCATC AGGCACCTGA GTCGCTGTCT TTTTCGTGAC ATTCAGTTCG CTGCGCTCAC GGCTCTGGCA GTGAATGGGG GTAAATGGCA CTACAGGCGC CTTTTATGGA TTCATGCAAG GAAACTACCC ATAATACAAG AAAAGCCCGT CACGGGCTTC TCAGGGCGTT TTATGGCGGG TCTGCTATGT GGTGCTATCT GACTTTTTGC TGTTCAGCAG TTCCTGCCCT CTGATTTTCC AGTCTGACCA CTTCGGATTA TCCCGTGACA GGTCATTCAG ACTGGCTAAT GCACCCAGTA AGGCAGCGGT ATCATCAACA GGCTTACCCG TCTTACTGTC GGGGATCGAC GCTCTCCCTT ATGCGACTCC TGCACCTTTC GTCTTCGAAT AAATACCTGT GACGGAAGAT CACTTCGCAG AATAAATAAA TCCTGGTGTC CCTGTTGATA CCGGGAAGCC CTGGGCCAAC TTTTGGCGAA AATGAGACGT TGATCGGCAC GTAAGAGGTT CCAACTTTCA CCATAATGAA ATAAGATCAC TACCGGGCGT ATTTTTTGAG TTATCGAGAT TTTCAGGAGC TAAGGAAGCT AAAATGGAGA AAAAAATCAC TGGATATACC ACCGTTGATA TATCCCAATG GCATCGTAAA GAACATTTTG AGGCATTTCA GTCAGTTGCT CAATGTACCT ATAACCAGAC CGTTCAGCTG GGTCATCCAC CGGATCAATT CCCCTGCTCG CGCAGGCTGG GTGCCAAGCT CTCGGGTAAC ATCAAGGCCC GATCCTTGGA GCCCTTGCCC TCCCGCACGA TGATCGTGCC GTGATCGAAA TCCAGATCCT TGACCCGCAG TTGCAAACCC TCACTGATCC GGCTCACGGT AACTGATGCC GTATTTGCAG TACCAGCGTA CGGCCCACAG AATGATGTCA CGCTGAAAAT GCCGGCCTTT GAATGGGTTC ATGTGCAGCT CCATCAGCAA AAGGGGATGA TAAGTTTATC ACCACCGACT ATTTGCAACA GTGCCGTTGA TCGTGCTATG ATCGACTGAT GTCATCAGCG GTGGAGTGCA ATGTCGTGCA ATACGAATGG CGAAAAGCCG AGCTCATCGG TCAGCTTCTC AACCTTGGGG TTACCCCCGG CGGTGTGCTG CTGGTCCACA GCTCCTTCCG TAGCGTCCGG CCCCTCGAAG ATGGGCCACT TGGACTGATC GAGGCCCTGC GTGCTGCGCT GGGTCCGGGA GGGACGCTCG TCATGCCCTC GTGGTCAGGT CTGGACGACG AGCCGTTCGA TCCTGCCACG TCGCCCGTTA CACCGGACCT TGGAGTTGTC TCTGACACAT TCTGGCGCCT GCCAAATGTA AAGCGCAGCG CCCATCCATT TGCCTTTGCG GCAGCGGGGC CACAGGCAGA GCAGATCATC TCTGATCCAT TGCCCCTGCC ACCTCACTCG CCTGCAAGCC CGGTCGCCCG TGTCCATGAA CTCGATGGGC AGGTACTTCT CCTCGGCGTG GGACACGATG CCAACACGAC GCTGCATCTT GCCGAGTTGA TGGCAAAGGT TCCCTATGGG GTGCCGAGAC ACTGCACCAT TCTTCAGGAT GGCAAGTTGG TACGCGTCGA TTATCTCGAG AATGACCACT GCTGTGAGCG CTTTGCCTTG GCGGACAGGT GGCTCAAGGA GAAGAGCCTT CAGAAGGAAG GTCCAGTCGG TCATGCCTTT GCTCGGTTGA TCCGCTCCCG CGACATTGTG GCGACAGCCC TGGGTCAACT GGGCCGAGAT CCGTTGATCT TCCTGCATCC GCCAGAGGCG GGATGCGAAG AATGCGATGC CGCTCGCCAG TCGATTGGCT GAGCTCATGA GCGGAGAACG AGATGACGTT GGAGGGGCAA GGTCGCGCTG ATTGCTGGGG CAACACGTGA AAGGCGAGAT CACCAAGGTA GTCGGCAAAT AATGTCTAAC GGTACCCGGG GATCCTCTAG AGTCGACCTG CATAATGTGC CTGTCAAATG GACGAAGCAG GGATTCTGCA AACCCTATGC TACTCCGTCA AGCCGTCAAT TGTCTGATTC GTTACCAATT ATGACAACTT GACGGCTACA TCATTCACTT TTTCTTCACA ACCGGCACGG AACTCGCTCG GGCTGGCCCC GGTGCATTTT TTAAATACCC GCGAGAAATA GAGTTGATCG TCAAAACCAA CATTGCGACC GACGGTGGCG ATAGGCATCC GGGTGGTGCT CAAAAGCAGC TTCGCCTGGC TGATACGTTG GTCCTCGCGC CAGCTTAAGA CGCTAATCCC TAACTGCTGG CGGAAAAGAT GTGACAGACG CGACGGCGAC AAGCAAACAT GCTGTGCGAC GCTGGCGATA TCAAAATTGC TGTCTGCCAG GTGATCGCTG ATGTACTGAC AAGCCTCGCG TACCCGATTA TCCATCGGTG GATGGAGCGA CTCGTTAATC GCTTCCATGC GCCGCAGTAA CAATTGCTCA AGCAGATTTA TCGCCAGCAG CTCCGAATAG CGCCCTTCCC CTTGCCCGGC GTTAATGATT TGCCCAAACA GGTCGCTGAA ATGCGGCTGG TGCGCTTCAT CCGGGCGAAA GAACCCCGTA TTGGCAAATA TTGACGGCCA GTTAAGCCAT TCATGCCAGT AGGCGCGCGG ACGAAAGTAA ACCCACTGGT GATACCATTC GCGAGCCTCC GGATGACGAC CGTAGTGATG AATCTCTCCT GGCGGGAACA GCAAAATATC ACCCGGTCGG CAAACAAATT CTCGTCCCTG ATTTTTCACC ACCCCCTGAC CGCGAATGGT GAGATTGAGA ATATAACCTT TCATTCCCAG CGGTCGGTCG ATAAAAAAAT CGAGATAACC GTTGGCCTCA ATCGGCGTTA AACCCGCCAC CAGATGGGCA TTAAACGAGT ATCCCGGCAG CAGGGGATCA TTTTGCGCTT CAGCCATACT TTTCATACTC CCGCCATTCA GAG
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