pCAG-Ace2(mouse)-FLAG
$84.00
In stock
SKU
PL38509
NM_001130513.1;2010305L05Rik
100μg liquid plasmid(Instantly):$105
| name | pCAG-Ace2(mouse)-FLAG | another name | apCAG-Ace2(mouse)-FLAG |
| Insert Size (bp) | 2415bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CAG | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | pcaggs-F：GTTCGGCTTCTGGCGTGT | |
| 3′ sequencing primer | pcaggs-R：TATGTCCTTCCGAGTGAGAG | Copy number | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC ATGGTCGAGG TGAGCCCCAC GTTCTGCTTC ACTCTCCCCA TCTCCCCCCC CTCCCCACCC CCAATTTTGT ATTTATTTAT TTTTTAATTA TTTTGTGCAG CGATGGGGGC GGGGGGGGGG GGGGGGCCCC CCCCAGGCGG GGCGGGGCGG GGCGAGGGGC GGGGCGGGGC GAGGCGGAAA GGTGCGGCGG CAGCCAATCA GAGCGGCGCG CTCCGAAAGT TTCCTTTTAT GGCGAGGCGG CGGCGGCGGC GGCCCTATAA AAAGCGAAGC GCGCGGCGGG CGGGAGTCGT TGCGCGCTGC CTTCCCCCCG TGCCCCGCTC CGCCGCCGCC TCGCGCCGCC CGCCCCGGCT CTGACTGACC GCGTTACTCC CACAGGTGAG CGGGCGGGAC GGCCCTTCTC CTCCGGGCTG TAATTAGCGC TTGGTTTAAT GACGGCTTGT TTCTTTTCTG TGGCTGCGTG AAAGCCTTGA GGGGCTCCGG GAGGGCCCTT TGTGCGGGGG GAGCGGCTCG GGGGGTGCGT GCGTGTGTGT GTGCGTGGGG AGCGCCGCGT GCGGCTCCGC GCTGCCCGGC GGCTGTGAGC GCTGCGGGCG CGGCGCGGGG CTTTGTGCGC TCCGCAGTGT GCGCGAGGGG AGCGCGGCCG GGGGCGGTGC CCCGCGGTGC GGGGGGGGCT GCGAGGGGAA CAAAGGCTGC GTGCGGGGTG TGTGCGTGGG GGGGTGAGCA GGGGGTGTGG GCGCGTCGGT CGGGCTGCAA CCCCCCCTGC ACCCCCCTCC CCGAGTTGCT GAGCACGGCC CGGCTTCGGG TGCGGGGCTC CGTACGGGGC GTGGCGCGGG GCTCGCCGTG CCGGGCGGGG GGTGGCGGCA GGTGGGGGTG CCGGGCGGGG CGGGGCCGCC TCGGGCCGGG GAGGGCTCGG GGGAAGGGGC GCGGCGGCCC CCGGAGCGCC GGCGGCTGTC GAGGCGCGGC GAGCCGCAGC CATTGCCTTT TATGGTAATC GTGCGAGAGG GCGCAGGGAC TTCCTTTGTC CCAAATCTGT GCGGAGCCGA AATCTGGGAG GCGCCGCCGC ACCCCCTCTA GCGGGCGCGG GGCGAAGCGG TGCGGCGCCG GCAGGAAGGA AATGGGCGGG GAGGGCCTTC GTGCGTCGCC GCGCCGCCGT CCCCTTCTCC CTCTCCAGCC TCGGGGCTGT CCGCGGGGGG ACGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT GTGACCGGCG GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA ACGTGCTGGT TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCCTCGAG CCACCATGTC CAGCTCCTCC TGGCTCCTTC TCAGCCTTGT TGCTGTTACT ACTGCTCAGT CCCTCACCGA GGAAAATGCC AAGACATTTT TAAACAACTT TAATCAGGAA GCTGAAGACC TGTCTTATCA AAGTTCACTT GCTTCTTGGA ATTATAATAC TAACATTACT GAAGAAAATG CCCAAAAGAT GAGTGAGGCT GCAGCCAAAT GGTCTGCCTT TTATGAAGAA CAGTCTAAGA CTGCCCAAAG TTTCTCACTA CAAGAAATCC AGACTCCGAT CATCAAGCGT CAACTACAGG CCCTTCAGCA AAGTGGGTCT TCAGCACTCT CAGCAGACAA GAACAAACAG TTGAACACAA TTCTGAACAC CATGAGCACC ATTTACAGTA CTGGAAAAGT TTGCAACCCA AAGAACCCAC AAGAATGCTT ATTACTTGAG CCAGGATTGG ATGAAATAAT GGCGACAAGC ACAGACTACA ACTCTAGGCT CTGGGCATGG GAGGGCTGGA GGGCTGAGGT TGGCAAGCAG CTGAGGCCGT TGTATGAAGA GTATGTGGTC CTGAAAAACG AGATGGCAAG AGCAAACAAT TATAACGACT ATGGGGATTA TTGGAGAGGG GACTATGAAG CAGAGGGAGC AGATGGCTAC AACTATAACC GTAACCAGTT GATTGAAGAT GTAGAACGTA CCTTCGCAGA GATCAAGCCA TTGTATGAGC ATCTTCATGC CTATGTGAGG AGGAAGTTGA TGGATACCTA CCCTTCCTAC ATCAGCCCCA CTGGATGCCT CCCTGCCCAT TTGCTTGGTG ATATGTGGGG TAGATTTTGG ACAAATCTGT ACCCTTTGAC TGTTCCCTTT GCACAGAAAC CAAACATAGA TGTTACTGAT GCAATGATGA ATCAGGGCTG GGATGCAGAA AGGATATTTC AAGAGGCAGA GAAATTCTTT GTTTCTGTTG GCCTTCCTCA TATGACTCAA GGATTCTGGG CAAACTCTAT GCTGACTGAG CCAGCAGATG GCCGGAAAGT TGTCTGCCAC CCCACAGCTT GGGATCTGGG ACACGGAGAC TTCAGAATCA AGATGTGTAC AAAGGTCACA ATGGACAACT TCTTGACAGC CCATCACGAG ATGGGACACA TCCAATATGA CATGGCATAT GCCAGGCAAC CTTTCCTGCT AAGAAACGGA GCCAATGAAG GGTTCCATGA AGCTGTTGGA GAAATCATGT CACTTTCTGC AGCTACCCCC AAGCATCTGA AATCCATTGG TCTTCTGCCA TCCGATTTTC AAGAAGATAG CGAAACAGAG ATAAACTTCC TACTGAAACA GGCATTGACA ATTGTTGGAA CACTACCGTT TACTTACATG TTAGAGAAGT GGAGGTGGAT GGTCTTTCGG GGTGAAATTC CCAAAGAGCA GTGGATGAAA AAGTGGTGGG AGATGAAGCG GGAGATCGTT GGTGTGGTGG AGCCTCTGCC TCATGATGAA ACATACTGTG ACCCTGCATC TCTGTTCCAT GTTTCTAATG ATTACTCATT CATTCGATAT TACACAAGGA CCATTTACCA ATTCCAGTTT CAAGAAGCTC TTTGTCAAGC AGCTAAGTAT AATGGTTCTC TGCACAAATG TGACATCTCA AATTCCACTG AAGCTGGGCA GAAGTTGCTC AAGATGCTGA GTCTTGGAAA TTCAGAGCCC TGGACCAAAG CCTTGGAAAA TGTGGTAGGA GCAAGGAATA TGGATGTAAA ACCACTGCTC AATTACTTCC AACCGTTGTT TGACTGGCTG AAAGAGCAGA ACAGAAATTC TTTTGTGGGG TGGAACACTG AATGGAGCCC ATATGCCGAC CAAAGCATTA AAGTGAGGAT AAGCCTAAAA TCAGCTCTTG GAGCTAATGC ATATGAATGG ACCAACAACG AAATGTTCCT GTTCCGATCA TCTGTTGCAT ATGCCATGAG AAAGTATTTT TCAATAATCA AAAACCAGAC AGTTCCTTTT CTAGAGGAAG ATGTACGAGT GAGTGATTTG AAACCAAGAG TCTCCTTCTA CTTCTTTGTC ACCTCACCCC AAAATGTGTC TGATGTCATT CCTAGAAGTG AAGTTGAAGA TGCCATCAGG ATGTCTCGGG GCCGCATCAA TGATGTCTTT GGCCTGAATG ATAACAGCCT GGAGTTTCTG GGGATTCACC CAACACTTGA GCCACCTTAC CAGCCTCCTG TCACCATATG GCTGATTATT TTTGGTGTTG TGATGGCACT GGTAGTGGTT GGCATCATCA TCCTGATTGT CACTGGGATC AAAGGTCGAA AGAAGAAAAA TGAAACAAAA AGAGAAGAGA ACCCTTATGA CTCGATGGAC ATTGGAAAAG GAGAAAGCAA TGCAGGATTC CAAAACAGTG ATGATGCTCA GACTTCCTTT GACTACAAGG ACGACGATGA CAAGTAGGAA TTCACTCCTC AGGTGCAGGC TGCCTATCAG AAGGTGGTGG CTGGTGTGGC CAATGCCCTG GCTCACAAAT ACCACTGAGA TCTTTTTCCC TCTGCCAAAA ATTATGGGGA CATCATGAAG CCCCTTGAGC ATCTGACTTC TGGCTAATAA AGGAAATTTA TTTTCATTGC AATAGTGTGT TGGAATTTTT TGTGTCTCTC ACTCGGAAGG ACATATGGGA GGGCAAATCA TTTAAAACAT CAGAATGAGT ATTTGGTTTA GAGTTTGGCA ACATATGCCC ATATGCTGGC TGCCATGAAC AAAGGTTGGC TATAAAGAGG TCATCAGTAT ATGAAACAGC CCCCTGCTGT CCATTCCTTA TTCCATAGAA AAGCCTTGAC TTGAGGTTAG ATTTTTTTTA TATTTTGTTT TGTGTTATTT TTTTCTTTAA CATCCCTAAA ATTTTCCTTA CATGTTTTAC TAGCCAGATT TTTCCTCCTC TCCTGACTAC TCCCAGTCAT AGCTGTCCCT CTTCTCTTAT GGAGATCCCT CGACCTGCAG CCCAAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCGGA TCCGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGATCC GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGG
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