pCMV-Notch1(rat)-8×His-Neo
$157.00
In stock
SKU
PL38500
NM_001105721.1;NOTCH; TAN1
100μg liquid plasmid(Instantly):$178
| name | pCMV-Notch1(rat)-8×His-Neo | another name | apCMV-Notch1(rat)-8×His-Neo |
| Insert Size (bp) | 7593bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Rat | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCATGCC GCGGCTCCTG GCGCCCTTGC TCTGCCTAAC GCTGCTGCCC GCGCTCGCCG CAAGAGGCTT GAGATGCTCC CAGCCAAGTG GGACCTGCCT GAATGGAGGG AGGTGCGAAG TGGCCAACGG CACTGAAGCC TGTGTCTGCA GCGGAGCGTT CGTGGGCCAG CGATGCCAGG ACCCCAGCCC TTGCCTCAGC ACACCATGTA AGAATGCTGG AACGTGCTAT GTTGTGGACC ATGGCGGCAT CGTGGACTAT GCCTGCAGTT GCCCCCTGGG TTTCTCTGGG CCCCTCTGCC TGACACCTCT GGCCAATGCC TGCCTGGCCA ACCCCTGCCG CAACGGGGGG ACCTGTGACC TGCTCACTCT CACAGAATAC AAGTGCCGGT GCCCGCCAGG GTGGTCAGGA AAGTCATGTC AGCAAGCCGA CCCCTGTGCC TCCAACCCCT GTGCCAATGG TGGCCAGTGC CTGCCCTTTG AGTCTTCATA CATCTGTGGC TGCCCGCCCG GCTTCCATGG CCCCACCTGC AGACAAGATG TTAACGAGTG CAGCCAGAAC CCTGGGTTGT GCCGTCATGG CGGCACGTGC CACAATGAGA TTGGCTCCTA TCGCTGTGCC TGCCGTGCCA CCCACACTGG TCCCCACTGC GAGCTGCCCT ACGTGCCCTG CAGCCCCTCA CCCTGCCAGA ACGGAGGCAC CTGCCGCCCT ACGGGGGACA CCACCCACGA GTGTGCCTGC CTGCCAGGCT TTGCTGGACA GAACTGTGAA GAAAATGTGG ATGACTGCCC AGGAAACAAC TGCAAGAACG GGGGTGCCTG TGTGGACGGT GTGAATACCT ACAATTGCCG CTGCCCACCG GAGTGGACAG GTCAGTACTG CACAGAGGAT GTGGACGAGT GTCAGCTCAT GCCCAACGCC TGCCAGAATG GCGGAACCTG CCACAACTCC CACGGTGGCT ACAACTGCGT GTGTGTCAAT GGCTGGACTG GTGAGGACTG CAGTGAGAAC ATTGATGACT GTGCCAGTGC CGCCTGTTTT CAGGGTGCCA CCTGCCATGA CCGTGTGGCT TCCTTCTACT GCGAGTGTCC ACATGGGCGC ACAGGCCTGC TGTGCCACCT GAACGATGCG TGTATCAGCA ACCCCTGCAA CGAGGGCTCC AACTGCGACA CCAACCCTGT CAACGGCAAG GCCATCTGCA CTTGCCCCTC GGGGTACACG GGGCCAGCCT GCAGCCAGGA CGTGGATGAG TGCGCTCTAG GTGCCAACCC GTGTGAGCAC GCGGGCAAGT GCCTCAACAC ACTGGGCTCT TTCGAGTGTC AGTGTCTACA GGGCTACACT GGGCCCCGCT GTGAGATTGA TGTCAACGAG TGCATCTCCA ACCCATGTCA GAATGATGCC ACGTGCCTGG ACCAGATTGG GGAGTTTCAG TGTATATGTA TGCCAGGTTA TGAGGGTGTA TACTGTGAGA TCAACACGGA CGAGTGTGCC AGCAGCCCCT GTCTACACAA TGGCCGCTGC GTGGACAAGA TCAACGAGTT CCTGTGTCAG TGTCCCAAAG GCTTCAGCGG GCACCTGTGC CAGTATGACG TGGATGAGTG CGCCAGCACA CCATGCAAGA ACGGCGCCAA GTGCCTGGAT GGGCCCAACA CCTACACCTG CGTGTGCACA GAAGGTTACA CGGGGACCCA CTGCGAGGTG GACATTGACG AGTGTGACCC TGACCCCTGT CACTATGGTT TGTGCAAGGA TGGTGTGGCC ACCTTTACCT GCCTCTGCCA GCCAGGCTAC ACAGGCCATC ACTGTGAGAC CAACATTAAT GAGTGTCACA GCCAGCCGTG CCGCCATGGC GGCACCTGCC AGGACCGTGA CAACTACTAC CTCTGCTTAT GCCTCAAGGG GACCACAGGA CCCAACTGTG AGATCAATCT GGATGACTGT GCGAGCAACC CCTGTGACTC TGGCACGTGT CTGGACAAGA TCGATGGCTA CGAGTGTGCG TGCGAGCCAG GCTACACAGG GAGCATGTGT AATGTCAACA TTGACGAATG TGCGGGCAGC CCCTGCCACA ACGGGGGCAC CTGTGAGGAT GGCATCGCCG GCTTCACTTG CCGCTGCCCC GAGGGCTACC ACGACCCTAC GTGCCTGTCT GAGGTCAACG AGTGCAACAG TAACCCCTGC ATCCATGGAG CTTGCCGGGA TGGCCTCAAT GGATACAAAT GTGACTGTGC CCCTGGGTGG AGTGGGACAA ACTGTGACAT CAACAACAAT GAGTGTGAGT CCAACCCTTG TGTCAACGGT GGCACCTGCA AAGACATGAC CAGTGGCTAC GTATGCACCT GCCGAGAAGG CTTCAGTGGC CCTAACTGCC AGACCAACAT TAACGAATGT GCTTCCAACC CCTGCCTGAA CCAGGGCACC TGCATTGATG ATGTCGCTGG GTACAAATGC AACTGCCCTC TGCCCTATAC AGGAGCCACA TGTGAGGTGG TGTTGGCCCC ATGTGCCACC AGCCCCTGCA AAAACAGTGG GGTATGCAAG GAGTCTGAGG ACTATGAGAG CTTTTCCTGT GTCTGTCCCA CAGGCTGGCA AGGTCAAACC TGCGAGATCG ACATCAATGA GTGTGTGAAA AGCCCGTGTC GCCATGGTGC CTCTTGCCAG AACACCAATG GCAGCTACCG CTGCCTCTGC CAGGCTGGCT ACACGGGTCG CAACTGCGAG AGTGACATCG ATGACTGCCG ACCCAACCCA TGTCACAACG GGGGTTCCTG CACTGACGGG GTCAACGCGG CCTTCTGCGA CTGCCTGCCC GGCTTCCAGG GTGCCTTCTG TGAGGAGGAC ATCAACGAAT GCGCCAGCAA TCCATGCCAA AATGGCGCCA ACTGCACTGA CTGCGTGGAC AGCTACACGT GCACCTGCCC CACGGGCTTC AATGGCATCC ATTGCGAGAA CAACACACCT GACTGTACCG AGAGCTCCTG TTTCAATGGT GGCACCTGTG TGGATGGTAT CAACTCCTTC ACCTGTCTGT GCCCACCTGG CTTCACGGGC AGCTACTGCC AGTATGACGT CAATGAGTGT GACTCACGGC CCTGTCTGCA TGGTGGCACC TGCCAAGACA GCTATGGTAC CTATAAGTGT ACCTGCCCAC AGGGCTACAC TGGTCTCAAC TGCCAGAACC TTGTGCGCTG GTGTGACTCA GCTCCCTGCA AGAATGGCGG CAAGTGCTGG CAGACCAACA CACAGTACCA CTGCGAGTGC CGCAGCGGCT GGACTGGCTT CAACTGCGAC GTGCTCAGTG TGTCCTGCGA GGTGGCTGCA CAGAAGCGAG GCATCGATGT CACTCTCCTA TGCCAGCACG GAGGGCTCTG TGTGGATGAG GAAGACAAGC ATTACTGCCA CTGCCAGGCA GGATACACGG GCAGCTACTG TGAGGACGAG GTGGACGAGT GCTCACCTAA TCCCTGCCAG AACGGAGCCA CCTGCACTGA CTATCTCGGT GGCTTTTCCT GCAAGTGTGT GGCTGGGTAC CATGGCTCTA ACTGCTCTGA GGAGATCAAC GAGTGCCTAT CCCAACCCTG CCAGAATGGG GGTACCTGCA TTGATCTGAC CAACACCTAC AAGTGCTCCT GCCCCAGGGG CACACAGGGT GTACACTGTG AGATCAACGT CGATGACTGC CATCCTCCCC TAGACCCTGC TTCCCGAAGC CCCAAATGCT TCAATAATGG CACCTGCGTG GACCAGGTGG GTGGCTATAC CTGCACCTGC CCGCCAGGCT TCGTCGGGGA GCGGTGCGAG GGCGATGTCA ATGAGTGTCT CTCCAACCCC TGTGACCCAC GTGGCACCCA GAACTGCGTG CAGCGTGTTA ATGACTTCCA CTGCGAGTGC CGGGCTGGCC ACACTGGACG CCGCTGTGAG TCGGTCATTA ATGGCTGCAG GGGCAAACCA TGCAGGAATG GAGGTGTCTG TGCTGTGGCC TCCAACACCG CCCGTGGATT CATCTGTAGG TGCCCTGCGG GCTTCGAGGG TGCCACTTGT GAAAATGACG CCCGCACTTG TGGCAGTTTG CGCTGCCTCA ACGGTGGTAC GTGCATCTCA GGCCCACGCA GTCCCACCTG CCTATGCCTG GGCTCCTTCA CTGGCCCTGA ATGCCAGTTC CCAGCCAGCA GCCCCTGTGT GGGTAGCAAC CCCTGCTACA ATCAGGGCAC CTGTGAGCCC ACATCCGAGA GCCCTTTCTA CCGCTGTCTA TGCCCTGCCA AATTCAACGG GCTGCTGTGC CACATCCTGG ACTACAGCTT CACAGGTGGC GCCGGGCGCG ACATTCCCCC GCCACAGATT GAGGAGGCCT GCGAACTGCC TGAGTGCCAG GAGGATGCAG GCAATAAGGT CTGCAACCTT CAGTGTAATA ATCACGCGTG TGGCTGGGAC GGCGGCGACT GCTCCCTCAA CTTCAATGAC CCTTGGAAGA ACTGCACTCA GTCCCTGCAG TGCTGGAAGT ATTTTAGTGA CGGCCACTGT GACAGCCAGT GCAACTCAGC TGGCTGCCTT TTTGATGGCT TCGACTGCCA GCTCACCGAG GGACAGTGCA ACCCCCTGTA TGACCAGTAC TGCAAGGACC ACTTCAGTGA TGGCCACTGC GACCAGGGCT GCAACAGTGC CGAGTGTGAG TGGGATGGAC TGGACTGTGC AGAGCATGTC CCCGAGCGAC TGGCAGCCGG CACACTGGTG CTGGTGGTGC TGCTTCCGCC CGATCAGCTA CGCAACAACT CCTTCCACTT CCTGCGGGAG CTCAGCCACG TGCTGCACAC CAACGTGGTC TTCAAGCGTG ACGCGCAGGG CCAGCAGATG ATCTTCCCCT ACTATGGCCG CGAGGAAGAG CTACGCAAGC ACCCGATCAA GCGTTCTGCA GTGGGCTGGG CCACAACGTC ACTGCTTCCT GGCACCAATG GTGGGCGCCA GCGCAGGGAG CTGGACCCCA TGGACATCCA TGGCTCCATC GTCTACCTGG AGATCGACAA CCGGCAGTGT GTACAGTCAT CCTCGCAATG CTTCCAGAGC GCCACCGATG TGGCTGCCTT CCTAGGTGCT CTTGCCTCCC TCGGCAGCCT CAATATCCCC TACAAGATCG AAGCCGTAAA GAGTGAGACG GTGGAGCCTC CGCTGCCCTC ACAGCTGCAC CTCATGTACG TGGCGGCAGC TGCCTTCGTG CTCCTGTTCT TTGTGGGCTG TGGGGTGCTG CTGTCCCGCA AGCGCAGGCG GCAGCATGGC CAGCTCTGGT TCCCTGAGGG TTTCAAAGTG TCAGAGGCCA GCAAGAAGAA GCGGAGAGAA CCCCTCGGCG AGGACTCAGT CGGCCTCAAG CCCCTGAAGA ACGCCTCAGA TGGTGCCCTG ATGGACGACA ATCAGAACGA GTGGGGGGAC GAAGACCTGG AGACCAAGAA GTTCCGGTTT GAGGAACCAG TGGTTCTCCC TGACCTGGAT GATCAGACTG ACCACCGGCA GTGGACCCAG CAGCACCTGG ATGCCGCTGA CCTACGTGTG TCTGCCATGG CCCCAACGCC GCCTCAGGGG GAGGTAGATG CTGACTGCAT GGACGTCAAT GTTCGAGGAC CAGATGGCTT CACTCCCCTA ATGATTGCCT CCTGCAGCGG AGGGGGCCTG GAGACAGGCA ACAGTGAGGA AGAAGAAGAT GCACCTGCTG TCATCTCCGA CTTCATCTAT CAGGGTGCCA GCTTGCACAA CCAGACGGAC CGCACAGGGG AGACTGCCCT GCACCTGGCT GCCCGATACT CTCGTTCAGA TGCTGCCAAG CGCTTGCTGG AGGCCAGCGC AGATGCCAAC ATCCAAGACA ACATGGGGCG TACCCCATTA CATGCCGCTG TTTCTGCAGA CGCTCAGGGT GTCTTCCAGA TCCTGCTCCG GAACAGAGCC ACAGATCTGG ATGCCCGAAT GCATGATGGC ACAACCCCTC TGATCCTGGC GGCACGCCTG GCCGTGGAAG GCATGCTAGA GGACCTCATC AACTCTCACG CTGATGTCAA TGCTGTGGAT GACCTAGGCA AGTCAGCTCT GCACTGGGCA GCCGCTGTGA ACAATGTGGA CGCTGCTGTT GTGCTCCTGA AGAACGGAGC CAACAAAGAC ATGCAGAACA ACAAGGAGGA GACTCCCCTG TTCCTGGCCG CCCGTGAGGG CAGCTATGAG ACTGCCAAAG TGTTGCTGGA CCACTTTGCC AACCGGGACA TCACGGATCA CATGGACCGA TTGCCACGGG ACATTGCACA GGAGCGCATG CACCACGATA TCGTGCGGCT TTTGGATGAA TACAACCTGG TGCGCAGCCC ACAGCTGCAT GGCACTGCCT TGGGTGGCAC ACCCACTCTG TCTCCCACAC TCTGCTCGCC CAACGGCTAC CTGGGCAACC TCAAGTCTGC CACACAGGGC AAGAAGGCCC GAAAGCCCAG CACCAAAGGG CTGGCTTGCA GTAGCAAGGA AGCTAAGGAC CTCAAGGCCC GGAGGAAGAA GTCCCAGGAT GGCAAGGGCT GCCTGTTGGA CAGCTCAAGC ATGCTGTCAC CCGTGGACTC CCTCGAGTCA CCCCATGGCT ACTTGTCAGA TGTGGCCTCA CCACCCCTCC TTCCCTCCCC GTTCCAGCAG TCTCCATCCA TGCCTCTCAG CCACCTGCCA GGTATGCCTG ACACCCACCT GGGCATCAGC CACTTGAATG TGGCAGCCAA GCCCGAGATG GCAGCTCTGG CCGGAGGCAG CCGGTTGGCC TTTGAGCCAC CCCCACCACG CCTCTCCCAC CTGCCTGTAG CCTCCAGTGC CAGCACAGTG CTGAGTACCA ATGGCACAGG GGCTATGAAT TTCACCGTGG GTGCACCGGC AAGCTTGAAT GGCCAGTGTG AGTGGCTTCC CCGGCTCCAG AATGGCATGG TGCCCAGCCA GTACAACCCG CTAAGGCCAG GTGTGACTCC GGGCACACTA AGCACACAGG CAGCTGGCCT CCAGCATGGC ATGATGGGCC CGATACACAG CAGCCTCTCC ACCAATACCT TGTCCCCGAT TATCTACCAG GGCCTGCCCA ACACAAGGCT GGCCACACAG CCCCACCTGG TGCAGACCCA GCAGGTGCAG CCACAGAACT TACAAATCCA GCCTCAGAAC CTGCAGCCAC CATCGCAGCC ACACCTCAGT GTGAGCTCAG CAGCCAATGG GCACCTGGGT CGGAGCTTCC TGAGCGGGGA GCCCAGCCAG GCAGACGTAC AGCCGCTGGG CCCCAGCAGT CTGCCTGTGC ACACCATTCT GCCCCAGGAA AGCCAGGCTC TGCCGACATC ACTGCCATCC TCCATGGTCC CACCCATGAC CACTACCCAG TTCCTGACCC CTCCTTCTCA GCACAGCTAC TCATCCTCAC CTGTGGACAA CACCCCCAGC CACCAGCTGC AGGTGCCAGA GCACCCCTTC CTCACCCCAT CCCCTGAGTC CCCTGACCAG TGGTCCAGCT CCTCCCCGCA TTCCAACATC TCTGATTGGT CCGAGGGCAT CTCTAGCCCG CCCACGAGCA TGCCGTCCCA GATCACCCAC ATTCCAGAGG CATTTAAGCT CGAGCACCAT CACCATCACC ATCACCATTA GGGCCCGATA TCTCGACAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAGCTGACGT CCTTTCCATG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCCTGG AAACGGGGGA GGCTAACTGA AACACGGAAG GAGACAATAC CGGAAGGAAC CCGCGCTATG ACGGCAATAA AAAGACAGAA TAAAACGCAC GGGTGTTGGG TCGTTTGTTC ATAAACGCGG GGTTCGGTCC CAGGGCTGGC ACTCTGTCGA TACCCCACCG AGACCCCATT GGGGCCAATA CGCCCGCGTT TCTTCCTTTT CCCCACCCCA CCCCCCAAGT TCGGGTGAAG GCCCAGGGCT CGCAGCCAAC GTCGGGGCGG CAGGCCCTGC CATAGCAGAT CTGCGCAGCT GGGGCTCTAG GGGGTATCCC CACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGCA TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGGGGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT AATTCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCAATGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCGAC GGATCGGGAG ATCTCCCGAT CCCCTATGGT CGACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GG
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