pSL4-CMV-3×Flag-CLK1(human)-Puro
$70.00
In stock
SKU
PL38468
NM_001162407.1;CLK; CLK/STY; STY
100μg liquid plasmid(Instantly):$91
| name | pSL4-CMV-3×Flag-CLK1(human)-Puro | another name | apSL4-CMV-3×Flag-CLK1(human)-Puro |
| Insert Size (bp) | 1578bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGGGTGGGAG TCATATAGCA GAGCTCGTTT AGTGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT TTTGACCTCC ATAGAAGACA CCGGCGGCCA CGGATCTGCC ACCATGGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA TTACAAGGAT GACGATGACA AGCTTGGATC CATGGCGGCT GGGCGGAGGC CGGCTTCGGC CCTGTGGCCG GAAAGGCGAG GCTCCCCGTT GAGGGGGGAT TTGCTGGGGT TCCAGAATGT GCGTGAGCCA AGCAGCTGTG GGGAAACGTT GTCTGGAATG AGACACTCAA AGAGAACTTA CTGTCCTGAT TGGGATGACA AGGATTGGGA TTATGGAAAA TGGAGGAGCA GCAGCAGTCA TAAAAGAAGG AAGAGATCAC ATAGCAGTGC CCAGGAGAAC AAGCGCTGCA AATACAATCA CTCTAAAATG TGTGATAGCC ATTATTTGGA AAGCAGGTCT ATAAATGAGA AAGATTATCA TAGTCGACGC TACATTGATG AGTACAGAAA TGACTACACT CAAGGATGTG AACCTGGACA TCGCCAAAGA GACCATGAAA GCCGGTATCA GAACCATAGT AGCAAGTCTT CTGGTAGAAG TGGAAGAAGT AGTTATAAAA GCAAACACAG GATTCACCAC AGTACTTCAC ATCGTCGTTC ACATGGGAAG AGTCACCGAA GGAAAAGAAC CAGGAGTGTA GAGGATGATG AGGAGGGTCA CCTGATCTGT CAGAGTGGAG ACGTACTAAG TGCAAGATAT GAAATTGTTG ATACTTTAGG TGAAGGAGCT TTTGGAAAAG TTGTGGAGTG CATCGATCAT AAAGCGGGAG GTAGACATGT AGCAGTAAAA ATAGTTAAAA ATGTGGATAG ATACTGTGAA GCTGCTCGCT CAGAAATACA AGTTCTGGAA CATCTGAATA CAACAGACCC CAACAGTACT TTCCGCTGTG TCCAGATGTT GGAATGGTTT GAGCATCATG GTCACATTTG CATTGTTTTT GAACTATTGG GACTTAGTAC TTACGACTTC ATTAAAGAAA ATGGTTTTCT ACCATTTCGA CTGGATCATA TCAGAAAGAT GGCATATCAG ATATGCAAGT CTGTGAATTT TTTGCACAGT AATAAGTTGA CTCACACAGA CTTAAAGCCT GAAAACATCT TATTTGTGCA GTCTGACTAC ACAGAGGCGT ATAATCCCAA AATAAAACGT GATGAACGCA CCTTAATAAA TCCAGATATT AAAGTTGTAG ACTTTGGTAG TGCAACATAT GATGACGAAC ATCACAGTAC ATTGGTATCT ACAAGACATT ATAGAGCACC TGAAGTTATT TTAGCCCTAG GGTGGTCCCA ACCATGTGAT GTCTGGAGCA TAGGATGCAT TCTTATTGAA TACTATCTTG GGTTTACCGT ATTTCCAACA CACGATAGTA AGGAGCATTT AGCAATGATG GAAAGGATTC TTGGACCTCT ACCAAAACAT ATGATACAGA AAACCAGGAA ACGTAAATAT TTTCACCACG ATCGATTAGA CTGGGATGAA CACAGTTCTG CCGGCAGATA TGTTTCAAGA CGCTGTAAAC CTCTGAAGGA ATTTATGCTT TCTCAAGATG TTGAACATGA GCGTCTCTTT GACCTCATTC AGAAAATGTT GGAGTATGAT CCAGCCAAAA GAATTACTCT CAGAGAAGCC TTAAAGCATC CTTTCTTTGA CCTTCTGAAG AAAAGTATAT AGAATTCAGA TATCGGTTCT AGAGCTAGCC TCGAGCGATC CCTCCCCCCC CCCTAACGTT ACTGGCCGAA GCCGCTTGGA ATAAGGCCGG TGTGCGTTTG TCTATATGTT ATTTTCCACC ATATTGCCGT CTTTTGGCAA TGTGAGGGCC CGGAAACCTG GCCCTGTCTT CTTGACGAGC ATTCCTAGGG GTCTTTCCCC TCTCGCCAAA GGAATGCAAG GTCTGTTGAA TGTCGTGAAG GAAGCAGTTC CTCTGGAAGC TTCTTGAAGA CAAACAACGT CTGTAGCGAC CCTTTGCAGG CAGCGGAACC CCCCACCTGG CGACAGGTGC CTCTGCGGCC AAAAGCCACG TGTATAAGAT ACACCTGCAA AGGCGGCACA ACCCCAGTGC CACGTTGTGA GTTGGATAGT TGTGGAAAGA GTCAAATGGC TCTCCTCAAG CGTATTCAAC AAGGGGCTGA AGGATGCCCA GAAGGTACCC CATTGTATGG GATCTGATCT GGGGCCTCGG TGCACATGCT TTACATGTGT TTAGTCGAGG TTAAAAAAAC GTCTAGGCCC CCCGAACCAC GGGGACGTGG TTTTCCTTTG AAAAACACGA TGATAATATG GCCACACATA TGCATACCGA GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GAGCATGCGC AGTCGACATC GATAGATCCT AATCAACCTC TGGATTACAA AATTTGGAAA GATGTACCCG
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