pLenti-EF1a-Cacna1i(mouse)-EGFP-Puro
$142.00
In stock
SKU
PL38456
NM_001044308.2;Ca(V)3.3
100μg liquid plasmid(Instantly):$163
| name | pLenti-EF1a-Cacna1i(mouse)-EGFP-Puro | another name | apLenti-EF1a-Cacna1i(mouse)-EGFP-Puro |
| Insert Size (bp) | 6600bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | EF1a | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AATTTTAACA AAATATTAAC GCTTACAATT TCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GATCCCTATA CAGTTGAAGT CGGAAGTTTA CATACACCTT AGCCAAATAC ATTTAAACTC ACTTTTTCAC AATTCCTGAC ATTTAATCCT AGTAAAAATT CCCTGTCTTA GGTCAGTTAG GATCACCACT TTATTTTAAG AATGTGAAAT ATCAGAATAA TAGTAGAGAG AATGATTCAT TTCAGCTTTT ATTTCTTTCA TCACATTCCC AGTGGGTCAG AAGTTTACAT ACACTCGACT CCTCTGCAGA ATGCGGCGAT GTTTCGGTAA GGGGTCCCGA GTAGCTAGTT CATGGCAGCC AGCATATGGC ATATGTTGCC AAACTCTAAA CCAAATACTC ATTCTGATGT TTTAAATGAT TTGCCCTCCC ATATGTCCTT CCGAGTGAGA GACACAAAAA ATTCCAACAC ACTATTGCAA TGAAAATAAA TTTCCTTTAT TAGCCAGAAG TCAGATGCTC AAGGGGCTTC ATGATGTCCC CATAATTTTT GGCAGAGGGA AAAAGATCTC AGTGGTATTT GTGAGCCAGG GCATTGGCCA CACCAGCCAC CACCTTCTGA TAGGCAGCCT GCACCTGAGG AGTGAATTCT CAGGCACCGG GCTTGCGGGT CATGCACCAG GTGCGCGGTC CTTCGGGCAC CTCGACGTCG GCGGTGACGG TGAAGCCGAG CCGCTCGTAG AAGGGGAGGT TGCGGGGCGC GGAGGTCTCC AGGAAGGCGG GCACCCCGGC GCGCTCGGCC GCCTCCACTC CGGGGAGCAC GACGGCGCTG CCCAGACCCT TGCCCTGGTG GTCGGGCGAG ACTCCGACGG TGGCCAGGAA CCACGCGGGC TCCTTGGGCC GGTGCGGCGC CAGGAGGCCT TCCATCTGTT GCTGCGCGGC CAGCCGGGAA CCGCTCAACT CGGCCATGCG CGGGCCGATC TCGGCGAACA CCGCCCCCGC TTCGACGCTC TCCGGCGTGG TCCAGACCGC CACCGCGGCG CCGTCGTCCG CGACCCACAC CTTGCCGATG TCGAGCCCGA CGCGCGTGAG GAAGAGTTCT TGCAGCTCGG TGACCCGCTC GATGTGGCGG TCCGGATCGA CGGTGTGGCG CGTGGCGGGG TAGTCGGCGA ACGCGGCGGC GAGGGTGCGT ACGGCCCTGG GGACGTCGTC GCGGGTGGCG AGGCGCACCG TGGGCTTGTA CTCGGTCGGT CCAGGATTCT CTTCGACATC TCCGGCTTGT TTCAGCAGAG AGAAGTTTGT TGCGAATTCC TTGTACAGCT CGTCCATGCC GAGAGTGATC CCGGCGGCGG TCACGAACTC CAGCAGGACC ATGTGATCGC GCTTCTCGTT GGGGTCTTTG CTCAGGGCGG ACTGGGTGCT CAGGTAGTGG TTGTCGGGCA GCAGCACGGG GCCGTCGCCG ATGGGGGTGT TCTGCTGGTA GTGGTCGGCG AGCTGCACGC TGCCGTCCTC GATGTTGTGG CGGATCTTGA AGTTCACCTT GATGCCGTTC TTCTGCTTGT CGGCCATGAT ATAGACGTTG TGGCTGTTGT AGTTGTACTC CAGCTTGTGC CCCAGGATGT TGCCGTCCTC CTTGAAGTCG ATGCCCTTCA GCTCGATGCG GTTCACCAGG GTGTCGCCCT CGAACTTCAC CTCGGCGCGG GTCTTGTAGT TGCCGTCGTC CTTGAAGAAG ATGGTGCGCT CCTGGACGTA GCCTTCGGGC ATGGCGGACT TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG GGTAGCGGCT GAAGCACTGC ACGCCGTAGG TCAGGGTGGT CACGAGGGTG GGCCAGGGCA CGGGCAGCTT GCCGGTGGTG CAGATGAACT TCAGGGTCAG CTTGCCGTAG GTGGCATCGC CCTCGCCCTC GCCGGACACG CTGAACTTGT GGCCGTTTAC GTCGCCGTCC AGCTCGACCA GGATGGGCAC CACCCCGGTG AACAGCTCCT CGCCCTTGCT CAACATGGTG GCCTCAGGTG CAGAGGTTTC TACAGGGAAA GAGAGAAAGG CTACAGTTAG TTTGCTGTGA TGATGCTAAG TGCCGAGGTC AGATGTCTAT GGATTGTACA CGGAATCCCA ACGCAGGAAC CAAAAAACAC TCACCCAAAA AAATGGCGCT AAGGCCGAGA GAAAGGCGAA GTTCCGTCTA CGGCTATATA CCTTGAAACA GGGGCGGCAG AAGTGACGCA ACGCGGTTGC GCGGACGGCT TCCTGTGTTT TCATTGGTCG AAGCGTTCTG CGCGTGCGCA GAGATTCCCC AGGGAAGGCG GGCCCTCTTC CGAATGTCCA ATCCGGACAT TCTTCCGGTT GGACCTCCGC GGTCAGCGCT GTGCCCGGCG AGAGATCACG TGGGGCGCGG AGGCGGTGCT GCTGGGGCAC GGCCGTCCAG CCTCGGCGGC CATATTTTTG AGGGGCTGTT CATCTCGTTC ACACGCTCTG TCCGCCATGT TTGTGAGTGG AAGCGCCATT ACCCCTTCAA GCGACTGAAG GCTGCAGGGC CTCTGGTGGC CCGCATGGGG AGACCAGACC CGCCAGGCCC GCCTTTCCGC ACTCAGTCCG GGCTTACTTT ATTTTGTGAG ACAGGGTCTC GCCTAGAGGC TCCGGTGCCC GTCAGTGGGC AGAGCGCACA TCGCCCACAG TCCCCGAGAA GTTGGGGGGA GGGGTCGGCA ATTGAACCGG TGCCTAGAGA AGGTGGCGCG GGGTAAACTG GGAAAGTGAT GTCGTGTACT GGCTCCGCCT TTTTCCCGAG GGTGGGGGAG AACCGTATAT AAGTGCAGTA GTCGCCGTGA ACGTTCTTTT TCGCAACGGG TTTGCCGCCA GAACACAGGT AAGTGCCGTG TGTGGTTCCC GCGGGCCTGG CCTCTTTACG GGTTATGGCC CTTGCGTGCC TTGAATTACT TCCACGCCCC TGGCTGCAGT ACGTGATTCT TGATCCCGAG CTTCGGGTTG GAAGTGGGTG GGAGAGTTCG AGGCCTTGCG CTTAAGGAGC CCCTTCGCCT CGTGCTTGAG TTGAGGCTTG GCCTGGGCGC TGGGGCCGCC GCGTGCGAAT CTGGTGGCAC CTTCGCGCCT GTCTCGCTGC TTTCGATAAG TCTCTAGCCA TTTAAAATTT TTGATGACCT GCTGCGACGC TTTTTTTCTG GCAAGATAGT CTTGTAAATG CGGGCCAAGA TCTGCACACT GGTATTTCGG TTTTTGGGGC CGCGGGCGGC GACGGGGCCC GTGCGTCCCA GCGCACATGT TCGGCGAGGC GGGGCCTGCG AGCGCGGCCA CCGAGAATCG GACGGGGGTA GTCTCAAGCT GGCCGGCCTG CTCTGGTGCC TGGCCTCGCG CCGCCGTGTA TCGCCCCGCC CTGGGCGGCA AGGCTGGCCC GGTCGGCACC AGTTGCGTGA GCGGAAAGAT GGCCGCTTCC CGGCCCTGCT GCAGGGAGCT CAAAATGGAG GACGCGGCGC TCGGGAGAGC GGGCGGGTGA GTCACCCACA CAAAGGAAAA GGGCCTTTCC GTCCTCAGCC GTCGCTTCAT GTGACTCCAC GGAGTACCGG GCGCCGTCCA GGCACCTCGA TTAGTTCTCG AGCTTTTGGA GTACGTCGTC TTTAGGTTGG GGGGAGGGGT TTTATGCGAT GGAGTTTCCC CATACTGAGT GGGTGGAGAC TGAAGTTAGG CCAGCTTGGC ACTTGATGTA ATTCTCCTTG GAATTTGCCC TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA AGTTTTTTTC TTCCATTTAA GGTGTCGTGA AAACTACCCC AAGCTGGCCT CTGAGGCCAC CATGGCTGAC AGCAACTTAC CACCCTCATC TTCAGCAGCC CCGGACCCTG AGCCGGGAAT CACTGAGCAG CCGGGGCCCC GGAGTCCCCC TCCATCCCCT CCAGGCCTGG AGGAGCCATT GGATGGAACC AACCCTGACG TCCCGCATCC AGACCTGGCA CCCGTTGCCT TCTTCTGCCT GCGCCAGACC ACGAGCCCAC GGAACTGGTG CATCAAGATG GTATGTAACC CGTGGTTCGA GTGTGTGAGC ATGCTGGTCA TCCTGCTGAA CTGTGTGACC CTGGGCATGT ACCAGCCATG TGACGATATG GAGTGCCTCT CAGACCGCTG CAAGATCCTG CAGGTCTTTG ATGACTTCAT CTTCATCTTC TTCGCCATGG AGATGGTGCT TAAGATGGTG GCCCTGGGCA TTTTTGGCAA GAAGTGCTAC CTCGGGGACA CATGGAACCG CTTGGACTTC TTCATTGTCA TGGCAGGGAT GGTCGAGTAC TCTCTGGACC TACAGAACAT CAACCTGTCA GCCATCCGCA CTGTGCGTGT CCTGAGGCCT CTCAAAGCCA TCAACCGTGT TCCCAGCATG CGGATTCTGG TGAACCTGCT GCTGGACACG CTGCCCATGC TGGGAAACGT GCTCCTGCTC TGTTTCTTCG TCTTCTTCAT CTTTGGCATC ATCGGCGTGC AGCTCTGGGC AGGCCTGCTG CGGAACCGCT GCTTCCTGGA GGAGAACTTC ACCATACAAG GGGATGTGGC CCTGCCCCCT TACTACCAAC CAGAAGAGGA CGACGAGATG CCCTTCATCT GCTCCCTGTC GGGGGACAAT GGCATTATGG GCTGTCACGA GATCCCTCCA CTCAAGGAGC AGGGCCGAGA GTGCTGCCTG TCCAAAGATG ACATGTACGA CTTCGGGGCG GGACGCCAAG ACCTCAACGC CAGCGGCCTC TGCGTCAACT GGAACCGCTA CTACAACGTC TGCCGCACGG GCAACGCCAA CCCTCACAAG GGCGCCATCA ACTTTGACAA CATTGGCTAT GCCTGGATCG TGATCTTTCA GGTGATCACT CTGGAAGGCT GGGTGGAGAT CATGTACTAC GTGATGGATG CACATTCTTT CTACAACTTC ATCTACTTCA TCCTGCTCAT CATAGTGGGC TCCTTCTTCA TGATCAACCT GTGCCTCGTT GTCATAGCGA CCCAGTTCTC TGAGACCAAG CAACGGGAGC ACCGGCTGAT GCTGGAGCAA CGCCAGCGCT ACCTGTCCTC CAGCACGGTG GCCAGTTACG CCGAGCCCGG CGACTGCTAT GAGGAGATCT TCCAATATGT CTGTCACATC CTTCGCAAGG CCAAGCGCCG TGCTCTAGGC CTCTACCAGG CCCTGCAGAA CCGGCGCCAG GCCACGGGCC CCGGGACACC AGCCCCTGCC AAGCCTGGGC CCCATGCCAA GGAGCCCAGC CACTGCAAGC TGTGCCCACG ACACAGTCCC CTGGACACCA CTCCCCACAC ACTGGTGCAG CCCATCTCTG CCATTCTGGC CTCCGATCCC AGCAGCTGCC CTCGCTGCCA GCACGAGGCA GGCAGGCGGC CCTCTGGCCT GGGCAGCACT GACTCGGGCC AGGAAGGCTC AGGTTCTGGC GGTTCTGCAG AGGCTGAAGC CAATGGCGAT GGACCCCAAA GCAGTGAGGA TGGGGTCTCC TCAGGTTTGG GGAAGGAGGA GGAACAGGAG GACGGGGCAG CCCGACTGTG TGGGGATGTG TGGCGAGAGA CACGGGCCAA GCTGCGGGGC ATTGTGGACA GCAAGTACTT CAACAGAGGC ATCATGATGG CTATTCTGGT TAACACTGTC AGCATGGGCA TCGAGCACCA TGAACAGCCA GAGGAACTGA CCAACATCCT GGAGATCTGC AACGTGGTCT TCACCAGCAT GTTTGCCCTG GAGATGATTC TGAAGCTGGC TGCCTTTGGG CTCTTTGACT ACCTGCGGAA CCCTTACAAC ATCTTCGACA GCATCATTGT CATCATCAGC ATCTGGGAAA TCGTGGGGCA GGCTGACGGC GGCCTGTCCG TGCTGCGCAC CTTCCGGCTG CTGCGGGTGC TGAAGCTGGT GCGCTTCATG CCGGCGCTGC GGCGCCAGCT CGTGGTGCTC ATGAAGACCA TGGACAACGT GGCCACCTTC TGCATGCTGC TCATGCTCTT CATTTTCATC TTCAGCATCC TTGGGATGCA TATCTTTGGC TGCAAATTCA GCCTCCGCAC GGACACTGGA GACACAGTCC CTGACAGGAA GAACTTCGAT TCCTTACTGT GGGCCATCGT CACCGTGTTT CAGATCCTCA CTCAGGAGGA CTGGAACGTT GTCCTGTACA ATGGCATGGC CTCCACCACC CCCTGGGCCT CCCTCTATTT TGTTGCCCTC ATGACCTTTG GCAACTATGT TCTCTTCAAT CTCCTGGTGG CTATCCTGGT CGAGGGCTTC CAGGCTGAGG GTGATGCTAA TCGCTCCTAC TCAGACGAGG ACCAGAGCTC ATCCAACTTG GAGGAGCTTG ATAAGCTCCC AGAGGGCCTG GACAGCAGCA GAGATCTCAA GCTCTGCCCA ATACCCATGA CACCCAATGG ACACCTGGAC CCTAGTCTCC CTCTGGGTGG ACATCTGGGT CCTGCTGGTG CCATGGGTGC TGCCCCCCGC CTCTCACTGC AGCCAGACCC AGTGCTGGTG GCCCTAGAAT CCCGGAAAAG CAGTGTCATG TCACTGGGCA GGATGAGCTA TGATCAGCGA TCTTTGTCCA GCTCCCGGAG CTCCTACTAT GGGCCCTGGG GCCGCAGCGG GACCTGGGCC AGCCGCCGCT CCAGCTGGAA CAGCCTGAAA CACAAGCCAC CCTCAGCTGA GCACGAGTCC CTGCTCTCCG GGGAGCGTGG TGGTAGTTGC GTCAGGGCCT GTGAAGGCGC CCGGGAGGAT GCGCCACCTC GCGCTGCACC CCTGCATGCC CCGCACACCC ACCACGCGCA TCATGGACCC CACTTGGCAC ACCGTCACCG CCACCACCGT CGGACACTGT CCCTTGATAC CAGGGACTCT GTTGACCTGG CAGAGCTGGT GCCCGTGGTG GGTGCCCACT CGCGGGCCGC TTGGAGGGCG GCAGGCCAGG CCCCTGGGCA CGAGGACTGC AATGGCAGAA TGCCCAACAT AGCCAAGGAT GTCTTCACCA AGATGGACGA CCGCCGCGAC CGTGGGGAGG ACGAGGAGGA GATCGACTAC ACCCTGTGTT TCCGCGTCCG CAAGATGATT GATGTGTACA AGCCTGACTG GTGCGAAGTC CGCGAGGACT GGTCGGTCTA CCTCTTCTCC CCTGAGAACA AGTTCCGGAT CCTGTGTCAG ACCATTATTG CTCACAAGCT TTTTGACTAC GTGGTCTTGG CCTTCATCTT CCTCAACTGT ATCACCATTG CTCTGGAGAG GCCCCAGATC GAAGCTGGTA GCACTGAGCG CATCTTCCTC ACGGTGTCTA ACTACATCTT CACAGCCATC TTTGTGGGCG AGATGACACT GAAGGTGGTC TCTTTGGGCC TATACTTCGG TGAGCAAGCA TACTTGCGTA GCAGCTGGAA CGTCCTGGAT GGTTTCCTGG TCTTTGTGTC TATCATCGAT ATCGTGGTAT CCGTGGCCTC TGCAGGGGGA GCCAAGATCC TGGGGGTCCT CCGGGTCCTG CGGCTCCTGC GTACCTTACG TCCTTTGAGG GTCATCAGCC GGGCCCCTGG GCTGAAGCTG GTGGTAGAGA CGCTCATCTC CTCCCTCAAG CCCATTGGAA ACATTGTCCT CATCTGCTGC GCCTTCTTCA TCATCTTCGG TATCCTGGGG GTGCAGCTTT TCAAAGGCAA GTTCTATCAC TGTCTGGGAG TGGACACCCG AAACATCACC AACCGATCAG ACTGTGTGGC CGCCAACTAC CGCTGGGTGC ATCACAAATA CAACTTTGAT AACCTGGGCC AGGCTTTGAT GTCCCTCTTT GTCTTGGCCT CCAAGGATGG CTGGGTGAAC ATCATGTATA ATGGATTGGA TGCTGTTGCT GTGGACCAGC AGCCGGTGAC AAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCCTTC CTGCTCATCG TCAGCTTCTT CGTGCTCAAC ATGTTCGTGG GAGTGGTCGT GGAGAACTTC CACAAGTGCC GGCAGCATCA GGAGGCTGAG GAGGCACGGC GGCGTGAGGA GAAACGGCTA CGGCGCCTGG AAAAGAAGCG CCGTAAGGCT CAGAGGCTGC CCTACTATGC CACCTACTGT CCCACAAGAC TGCTCATCCA CTCCATGTGC ACTAGCCACT ACCTGGACAT CTTCATCACC TTCATCATCT GCCTCAACGT GGTCACCATG TCCCTGGAGC ACTACAACCA GCCTACATCC CTAGAGACAG CCCTCAAGTA CTGCAACTAC ATGTTCACCA CTGTCTTTGT GCTGGAGGCT GTGCTGAAGC TGGTGGCGTT TGGCCTGAGG CGTTTCTTCA AGGACCGATG GAACCAGCTG GACTTGGCCA TTGTGCTGCT GTCTGTCATG GGCATCACAC TGGAGGAGAT TGAGATCAAT GCGGCCCTGC CCATCAACCC CACCATCATC CGTATCATGC GTGTTCTGCG TATTGCCCGG GTGTTGAAGC TGTTGAAGAT GGCCACAGGA ATGCGGGCCC TGCTGGACAC AGTGGTACAG GCTCTGCCCC AGGTGGGCAA CCTGGGCCTC CTCTTCATGC TGCTCTTCTT CATCTACGCC GCTCTGGGAG TGGAGCTCTT CGGAAAGCTG GTCTGCAATG ACGAGAACCC GTGTGAGGGC ATGAGCCGGC ACGCCACCTT CGAGAACTTC GGCATGGCCT TCCTCACACT CTTCCAGGTC TCCACAGGCG ATAACTGGAA TGGGATTATG AAGGACACTC TGCGAGACTG CACCCACGAC GAGCGCAGCT GCCTGAGCAG CCTGCAGTTT GTGTCGCCTC TCTACTTTGT GAGCTTCGTA CTCACAGCGC AGTTTGTGCT CATCAACGTG GTGGTGGCCG TGCTGATGAA ACATCTGGAT GACAGCAACA AGGAGGCCCA GGAGGATGCA GAGATGGATG CTGAGATCGA GCTGGAGATG GCCCATGGCC TAGGCCCCGG GCCCGGCCCC TGTCCCTGTC CCTGTCCCTG CCCCTGCCCC TGTCCCTGTC CTGGCCCGAG GATGCCCACC AGTTCACCGG GGGCTCCAGG CCGAGGATCA GGAGGGGCAG GCGTTGGAGG CGACACTGAG AGCCACCTGT GCCGGCACTG CTATTCTCCA GCCCAGGAGA CCCTGTGGCT GGACAGCGTC TCTTTAATCA TCAAGGACTC CTTGGAGGGG GAGCTGACCA TCATTGACAA CCTGTCTGGC TCCATCTTCC ACCACTACTC CTCACCTGCC GGCTGTGACA AGTGTCACCA TGACAAGCAA GAGGTGCAGC TGGCTGAGAC AGAGGCCTTC TCCCTGAACT CAGACAGGTC CTCGTCCGTC CTGCTGGGGG ATGACCTGAG TCTTGAGGAC CCCACGGCCT GCCCGCAGGG CCCCAAGGAG AGCAAGGGTG AGCTAGAGCC TCCGGAGCCC ATGCAGGCTG GAGACCTGGA TGAATGCTTC TTCCCCTTTG CCGGTGAGCC AGTGTCTGCA GGCCCAGAGA GCCTGCTGTG CGAGATGGGG GCCATTCCAT TCAACCCCGT CCAGTCCTGG CTCAAACATG AGAGCAACCA AGCACCCCCA AGCCCTTTCT CCCCGGATGG TTCCAGCCCT CTGCTGCAGA TGCCTGCTGA GTTCTTCCAC CCTGCTGTGT CTGCCAGCCA GAAGGGGCAG GAACCGGGCA TGAGTTCAGG GACCCTGCCC AAGATTGCAC TTCAGGGGTC CTGGGCATCC CTGAGGTCAC CGAGTGTCAA CTGCACCCTC TTGCGCCAGG CTACTGTGAG TGACACGTCT TTGGATGCCA GCCCTAGCAG CTCAGCGGGC AGCCTGCAGA CCACACTGGA GGACAGTCTG ACCCTGAGTG ACAGTCCTCG GCGTGCCCTG GGACCACCAG TCCAGGTGCC AGGGCCACGG GCTAGCCTGT CACCTGCCAC CCGGCGCCGC CTCAGCCTGC GGGGGCGTGG CCTGTTTAGT CTGCGTGGGC TGCGGGCCCA TCAGCGTAGC CACAGCAGCG GTGGCTCCAC CAGCCCTGGC TGCACTTACC ACGACTCCAT GGACCCCTCT GATGAGGAGG GCCGCGGGGG CGCAGGTGGT GGGGGCGCGG GCAGTGAGCA CTCCGAGACC CTCAGCAGCC TGTCACTCAC ATCTCTCTTC TGCCTACCAC CCACGCTGCC ACCACCCGGT CTCACCCCAG CCAGGAAGTT CAGCAGTACC AGCAGCCTGG CTGCTGGACC TGGCCGTCCT GGCGCCACTG TCTCGGTCCG CGGCCTGGCC AGGAGTCCCT CATGGGCTGC AGACCGCAGC AAGGACCCAC CAGGCCAGGC ACAGCTGGCC TCCGGCTTCG GCTCCTCAGC ACCCGAGCCA CAGCCACCTC CCGGGGAGTC AACGGATGCG GCGAGCAAAA GGAAGAGATG AATCTAGAGC TCGCTGATCA GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA CCAGCTGGGC CTACTAGATA GCGGACCCCT TACCGAAACA TCGCCGCATT CTGCAGAGGA GTCGAACTTA GTGTATGTAA ACTTCTGACC CACTGGAATT GTGATACAGT GAATTATAAG TGAAATAATC TGTCTGTAAA CAATTGTTGG AAAAATGACT TGTGTCATGC ACAAAGTAGA TGTCCTAACT GACTTGCCAA AACTATTGTT TGTTAACAAG AAATTTGTGG AGTAGTTGAA AAACGAGTTT TAATGACTCC AACTTAAGTG TATGTAAACT TCCGACTTCA ACTGTATAGG GATCC
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