NM_001083619.3;GluA2; gluR-2; gluR-B; GluR-K2; GLUR2
100μg liquid plasmid(Instantly):$105
| name | pCMV-GRIA2(human)-CAG-mCherry-Neo | another name | apCMV-GRIA2(human)-CAG-mCherry-Neo |
| Insert Size (bp) | 2652bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | Red |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CAGCATATGG GCATATGTTG CCAAACTCTA AACCAAATAC TCATTCTGAT GTTTTAAATG ATTTGCCCTC CCATATGTCC TTCCGAGTGA GAGACACAAA AAATTCCAAC ACACTATTGC AATGAAAATA AATTTCCTTT ATTAGCCAGA AGTCAGATGC TCAAGGGGCT TCATGATGTC CCCATAATTT TTGGCAGAGG GAAAAAGATC TCTACTTGTA CAGCTCGTCC ATGCCGCCGG TGGAGTGGCG GCCCTCGGCG CGTTCGTACT GTTCCACGAT GGTGTAGTCC TCGTTGTGGG AGGTGATGTC CAACTTGATG TTGACGTTGT AGGCGCCGGG CAGCTGCACG GGCTTCTTGG CCTTGTAGGT GGTCTTGACC TCAGCGTCGT AGTGGCCGCC GTCCTTCAGC TTCAGCCTCT GCTTGATCTC GCCCTTCAGG GCGCCGTCCT CGGGGTACAT CCGCTCGGAG GAGGCCTCCC AGCCCATGGT CTTCTTCTGC ATTACGGGGC CGTCGGAGGG GAAGTTGGTG CCGCGCAGCT TCACCTTGTA GATGAACTCG CCGTCCTGCA GGGAGGAGTC CTGGGTCACG GTCACCACGC CGCCGTCCTC GAAGTTCATC ACGCGCTCCC ACTTGAAGCC CTCGGGGAAG GACAGCTTCA AGTAGTCGGG GATGTCGGCG GGGTGCTTCA CGTAGGCCTT GGAGCCGTAC ATGAACTGAG GGGACAGGAT GTCCCAGGCG AAGGGCAGGG GGCCACCCTT GGTCACCTTC AGCTTGGCGG TCTGGGTGCC CTCGTAGGGG CGGCCCTCGC CCTCGCCCTC GATCTCGAAC TCGTGGCCGT TCACGGAGCC CTCCATGTGC ACCTTGAAGC GCATGAACTC CTTGATGATG GCCATGTTAT CCTCCTCGCC CTTGCTCACC ATGAGCTCCC ACCTTCTGAT AGGAAGCTTC AGCCTGCACC TGCTAGCGAG GAGTGAATTC CTCGAGGAAT TCTTTGCCAA AATGATGAGA CAGCACAATA ACCAGCACGT TGCCCAGGAG CTGTAGGAAA AAGAAGAAGG CATGAACATG GTTAGCAGAG GCTCTAGAGC CGCCGGTCAC ACGCCAGAAG CCGAACCCCG CCCTGCCCCG TCCCCCCCGA AGGCAGCCGT CCCCCCGCGG ACAGCCCCGA GGCTGGAGAG GGAGAAGGGG ACGGCGGCGC GGCGACGCAC GAAGGCCCTC CCCGCCCATT TCCTTCCTGC CGGCGCCGCA CCGCTTCGCC CCGCGCCCGC TAGAGGGGGT GCGGCGGCGC CTCCCAGATT TCGGCTCCGC ACAGATTTGG GACAAAGGAA GTCCCTGCGC CCTCTCGCAC GATTACCATA AAAGGCAATG GCTGCGGCTC GCCGCGCCTC GACAGCCGCC GGCGCTCCGG GGGCCGCCGC GCCCCTTCCC CCGAGCCCTC CCCGGCCCGA GGCGGCCCCG CCCCGCCCGG CACCCCCACC TGCCGCCACC CCCCGCCCGG CACGGCGAGC CCCGCGCCAC GCCCCGTACG GAGCCCCGCA CCCGAAGCCG GGCCGTGCTC AGCAACTCGG GGAGGGGGGT GCAGGGGGGG TTGCAGCCCG ACCGACGCGC CCACACCCCC TGCTCACCCC CCCACGCACA CACCCCGCAC GCAGCCTTTG TTCCCCTCGC AGCCCCCCCC GCACCGCGGG GCACCGCCCC CGGCCGCGCT CCCCTCGCGC ACACTGCGGA GCGCACAAAG CCCCGCGCCG CGCCCGCAGC GCTCACAGCC GCCGGGCAGC GCGGAGCCGC ACGCGGCGCT CCCCACGCAC ACACACACGC ACGCACCCCC CGAGCCGCTC CCCCCGCACA AAGGGCCCTC CCGGAGCCCC TCAAGGCTTT CACGCAGCCA CAGAAAAGAA ACAAGCCGTC ATTAAACCAA GCGCTAATTA CAGCCCGGAG GAGAAGGGCC GTCCCGCCCG CTCACCTGTG GGAGTAACGC GGTCAGTCAG AGCCGGGGCG GGCGGCGCGA GGCGGCGGCG GAGCGGGGCA CGGGGGGAAG GCAGCGCGCA ACGACTCCCG CCCGCCGCGC GCTTCGCTTT TTATAGGGCC GCCGCCGCCG CCGCCTCGCC ATAAAAGGAA ACTTTCGGAG CGCGCCGCTC TGATTGGCTG CCGCCGCACC TTTCCGCCTC GCCCCGCCCC GCCCCTCGCC CCGCCCCGCC CCGCCTGGGG GGGGCCCCCC CCCCCCCCCC GCCCCCATCG CTGCACAAAA TAATTAAAAA ATAAATAAAT ACAAAATTGG GGGTGGGGAG GGGGGGGAGA TGGGGAGAGT GAAGCAGAAC GTGGGGCTCA CCTCGACCAT GGTAATAGCG ATGACTAATA CGTAGATGTA CTGCCAAGTA GGAAAGTCCC ATAAGGTCAT GTACTGGGCA TAATGCCAGG CGGGCCATTT ACCGTCATTG ACGTCAATAG GGGGCGTACT TGGCATATGA TACACTTGAT GTACTGCCAA GTGGGCAGTT TACCGTAAAT ACTCCACCCA TTGACGTCAA TGGAAAGTCC CTATTGGCGT TACTATGGGA ACATACGTCA TTATTGACGT CAATGGGCGG GGGTCGTTGG GCGGTCAGCC AGGCGGGCCA TTTACCGTAA GTTATGTAAC GCGGAACTCC ATATATGGGC TATGAACTAA TGACCCCGTA ATTGATTACT ATTAATAACT AGTCAATAAT CAATGTCGTG ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCCTGCAGT CGACGGTACC GCGGGCCCGG GATCCGCCAC CATGCAAAAG ATTATGCATA TTTCTGTCCT CCTTTCTCCT GTTTTATGGG GACTGATTTT TGGTGTCTCT TCTAACAGCA TACAGATAGG GGGGCTATTT CCTAGGGGCG CCGATCAAGA ATACAGTGCA TTTCGAGTAG GGATGGTTCA GTTTTCCACT TCGGAGTTCA GACTGACACC CCACATCGAC AATTTGGAGG TGGCAAACAG CTTCGCAGTC ACTAATGCTT TCTGCTCCCA GTTTTCGAGA GGAGTCTATG CTATTTTTGG ATTTTATGAC AAGAAGTCTG TAAATACCAT CACATCATTT TGCGGAACAC TCCACGTCTC CTTCATCACT CCCAGCTTCC CAACAGATGG CACACATCCA TTTGTCATTC AGATGAGACC CGACCTCAAA GGAGCTCTCC TTAGCTTGAT TGAATACTAT CAATGGGACA AGTTTGCATA CCTCTATGAC AGTGACAGAG GCTTATCAAC ACTGCAAGCT GTGCTGGATT CTGCTGCTGA AAAGAAATGG CAAGTGACTG CTATCAATGT GGGAAACATT AACAATGACA AGAAAGATGA GATGTACCGA TCACTTTTTC AAGATCTGGA GTTAAAAAAG GAACGGCGTG TAATTCTGGA CTGTGAAAGG GATAAAGTAA ACGACATTGT AGACCAGGTT ATTACCATTG GAAAACATGT TAAAGGGTAC CACTACATCA TTGCAAATCT GGGATTTACT GATGGAGACC TATTAAAAAT CCAGTTTGGA GGTGCAAATG TCTCTGGATT TCAGATAGTG GACTATGATG ATTCGTTGGT ATCTAAATTT ATAGAAAGAT GGTCAACACT GGAAGAAAAA GAATACCCTG GAGCTCACAC AACAACAATT AAGTATACTT CTGCTCTGAC CTATGATGCC GTTCAAGTGA TGACTGAAGC CTTCCGCAAC CTAAGGAAGC AAAGAATTGA AATCTCCCGA AGGGGGAATG CAGGAGACTG TCTGGCAAAC CCAGCAGTGC CCTGGGGACA AGGTGTAGAA ATAGAAAGGG CCCTCAAACA GGTTCAGGTT GAAGGTCTCT CAGGAAATAT AAAGTTTGAC CAGAATGGAA AAAGAATAAA CTATACAATT AACATCATGG AGCTCAAAAC TAATGGGCCC CGGAAGATTG GCTACTGGAG TGAAGTGGAC AAAATGGTTG TTACCCTTAC TGAGCTCCCT TCTGGAAATG ACACCTCTGG GCTTGAGAAT AAGACTGTTG TTGTCACCAC AATTTTGGAA TCTCCGTATG TTATGATGAA GAAAAATCAT GAAATGCTTG AAGGCAATGA GCGCTATGAG GGCTACTGTG TTGACCTGGC TGCAGAAATC GCCAAACATT GTGGGTTCAA GTACAAGTTG ACAATTGTTG GTGATGGCAA GTATGGGGCC AGGGATGCAG ACACGAAAAT TTGGAATGGG ATGGTTGGAG AACTTGTATA TGGGAAAGCT GATATTGCAA TTGCTCCATT AACTATTACC CTTGTGAGAG AAGAGGTGAT TGACTTCTCA AAGCCCTTCA TGAGCCTCGG GATATCTATC ATGATCAAGA AGCCTCAGAA GTCCAAACCA GGAGTGTTTT CCTTTCTTGA TCCTTTAGCC TATGAGATCT GGATGTGCAT TGTTTTTGCC TACATTGGGG TCAGTGTAGT TTTATTCCTG GTCAGCAGAT TTAGCCCCTA CGAGTGGCAC ACTGAGGAGT TTGAAGATGG AAGAGAAACA CAAAGTAGTG AATCAACTAA TGAATTTGGG ATTTTTAATA GTCTCTGGTT TTCCTTGGGT GCCTTTATGC AGCAAGGATG CGATATTTCG CCAAGATCCC TCTCTGGGCG CATTGTTGGA GGTGTGTGGT GGTTCTTTAC CCTGATCATA ATCTCCTCCT ACACGGCTAA CTTAGCTGCC TTCCTGACTG TAGAGAGGAT GGTGTCTCCC ATCGAAAGTG CTGAGGATCT TTCTAAGCAA ACAGAAATTG CTTATGGAAC ATTAGACTCT GGCTCCACTA AAGAGTTTTT CAGGAGATCT AAAATTGCAG TGTTTGATAA AATGTGGACC TACATGCGGA GTGCGGAGCC CTCTGTGTTT GTGAGGACTA CGGCCGAAGG GGTGGCTAGA GTGCGGAAGT CCAAAGGGAA ATATGCCTAC TTGTTGGAGT CCACGATGAA CGAGTACATT GAGCAAAGGA AGCCTTGCGA CACCATGAAA GTTGGTGGAA ACCTGGATTC CAAAGGCTAT GGCATCGCAA CACCTAAAGG ATCCTCATTA AGAAATGCGG TTAACCTCGC AGTACTAAAA CTGAATGAAC AAGGCCTGTT GGACAAATTG AAAAACAAAT GGTGGTACGA CAAAGGAGAG TGCGGCAGCG GGGGAGGTGA TTCCAAGGAA AAGACCAGTG CCCTCAGTCT GAGCAACGTT GCTGGAGTAT TCTACATCCT TGTCGGGGGC CTTGGTTTGG CAATGCTGGT GGCTTTGATT GAGTTCTGTT ACAAGTCAAG GGCCGAGGCG AAACGAATGA AGGTGGCAAA GAATGCACAG AATATTAACC CATCTTCCTC GCAGAATTCA CAGAATTTTG CAACTTATAA GGAAGGTTAC AACGTATATG GCATCGAAAG TGTTAAAATT TAGGCGGCCG CGACTCTAGA TCATAATCAG CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA CCTGAAACAT AAAATGAATG CAATTGTTGT TGTTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTAAGCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTC CTGAGGCGGA AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAGA TCGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCT AGGGGGAGGC TAACTGAAAC ACGGAAGGAG ACAATACCGG AAGGAACCCG CGCTATGACG GCAATAAAAA GACAGAATAA AACGCACGGT GTTGGGTCGT TTGTTCATAA ACGCGGGGTT CGGTCCCAGG GCTGGCACTC TGTCGATACC CCACCGAGAC CCCATTGGGG CCAATACGCC CGCGTTTCTT CCTTTTCCCC ACCCCACCCC CCAAGTTCGG GTGAAGGCCC AGGGCTCGCA GCCAACGTCG GGGCGGCAGG CCCTGCCATA GCCTCAGGTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGT
