NM_001165963.4;DEE6; DEE6A; DEE6B; DRVT; EIEE6; FEB3;FEB3A; FHM3; GEFSP2; HBSCI; NAC1; Nav1.1; SCN1; SMEI
100μg liquid plasmid(Instantly):$163
| name | pCMV-SCN1A(human)-EGFP-Neo | another name | apCMV-SCN1A(human)-EGFP-Neo |
| Insert Size (bp) | 6027bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | BL21(DE3) | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG TACCGAGCTC GGATCCGCCA CCATGGAGCA AACAGTGCTT GTACCACCAG GACCTGACAG CTTCAACTTC TTCACCAGAG AATCTCTTGC GGCTATTGAA AGACGCATTG CAGAAGAAAA GGCAAAGAAT CCCAAACCAG ACAAAAAAGA TGACGACGAA AATGGCCCAA AGCCAAATAG TGACTTGGAA GCTGGAAAGA ACCTTCCATT TATTTATGGA GACATTCCTC CAGAGATGGT GTCAGAGCCC CTGGAGGACC TGGACCCCTA CTATATCAAT AAGAAAACTT TTATAGTATT GAATAAAGGG AAGGCCATCT TCCGGTTCAG TGCCACCTCT GCCCTGTACA TTTTAACTCC CTTCAATCCT CTTAGGAAAA TAGCTATTAA GATTTTGGTA CATTCATTAT TCAGCATGCT AATTATGTGC ACTATTTTGA CAAACTGTGT GTTTATGACA ATGAGTAACC CTCCTGATTG GACAAAGAAT GTAGAATACA CCTTCACAGG AATATATACT TTTGAATCAC TTATAAAAAT TATTGCAAGG GGATTCTGTT TAGAAGATTT TACTTTCCTT CGGGATCCAT GGAACTGGCT CGATTTCACT GTCATTACAT TTGCGTACGT CACAGAGTTT GTGGACCTGG GCAATGTCTC GGCATTGAGA ACATTCAGAG TTCTCCGAGC ATTGAAGACG ATTTCAGTCA TTCCAGGCCT GAAAACCATT GTGGGAGCCC TGATCCAGTC TGTGAAGAAG CTCTCAGATG TAATGATCCT GACTGTGTTC TGTCTGAGCG TATTTGCTCT AATTGGGCTG CAGCTGTTCA TGGGCAACCT GAGGAATAAA TGTATACAAT GGCCTCCCAC CAATGCTTCC TTGGAGGAAC ATAGTATAGA AAAGAATATA ACTGTGAATT ATAATGGTAC ACTTATAAAT GAAACTGTCT TTGAGTTTGA CTGGAAGTCA TATATTCAAG ATTCAAGATA TCATTATTTC CTGGAGGGTT TTTTAGATGC ACTACTATGT GGAAATAGCT CTGATGCAGG CCAATGTCCA GAGGGATATA TGTGTGTGAA AGCTGGTAGA AATCCCAATT ATGGCTACAC AAGCTTTGAT ACCTTCAGTT GGGCTTTTTT GTCCTTGTTT CGACTAATGA CTCAGGACTT CTGGGAAAAT CTTTATCAAC TGACATTACG TGCTGCTGGG AAAACGTACA TGATATTTTT TGTATTGGTC ATTTTCTTGG GCTCATTCTA CCTAATAAAT TTGATCCTGG CTGTGGTGGC CATGGCCTAC GAGGAACAGA ATCAGGCCAC CTTGGAAGAA GCAGAACAGA AAGAGGCCGA ATTTCAGCAG ATGATTGAAC AGCTTAAAAA GCAACAGGAG GCAGCTCAGC AGGCAGCAAC GGCAACTGCC TCAGAACATT CCAGAGAGCC CAGTGCAGCA GGCAGGCTCT CAGACAGCTC ATCTGAAGCC TCTAAGTTGA GTTCCAAGAG TGCTAAGGAA AGAAGAAATC GGAGGAAGAA AAGAAAACAG AAAGAGCAGT CTGGTGGGGA AGAGAAAGAT GAGGATGAAT TCCAAAAATC TGAATCTGAG GACAGCATCA GGAGGAAAGG TTTTCGCTTC TCCATTGAAG GGAACCGATT GACATATGAA AAGAGGTACT CCTCCCCACA CCAGTCTTTG TTGAGCATCC GTGGCTCCCT ATTTTCACCA AGGCGAAATA GCAGAACAAG CCTTTTCAGC TTTAGAGGGC GAGCAAAGGA TGTGGGATCT GAGAACGACT TCGCAGATGA TGAGCACAGC ACCTTTGAGG ATAACGAGAG CCGTAGAGAT TCCTTGTTTG TGCCCCGACG ACACGGAGAG AGACGCAACA GCAACCTGAG TCAGACCAGT AGGTCATCCC GGATGCTGGC AGTGTTTCCA GCGAATGGGA AGATGCACAG CACTGTGGAT TGCAATGGTG TGGTTTCCTT GGTTGGTGGA CCTTCAGTTC CTACATCGCC TGTTGGACAG CTTCTGCCAG AGGTGATAAT AGATAAGCCA GCTACTGATG ACAATGGAAC AACCACTGAA ACTGAAATGA GAAAGAGAAG GTCAAGTTCT TTCCACGTTT CCATGGACTT TCTAGAAGAT CCTTCCCAAA GGCAACGAGC AATGAGTATA GCCAGCATTC TAACAAATAC AGTAGAAGAA CTTGAAGAAT CCAGGCAGAA ATGCCCACCC TGTTGGTATA AATTTTCCAA CATATTCTTA ATCTGGGACT GTTCTCCATA TTGGTTAAAA GTGAAACATG TTGTCAACCT GGTTGTGATG GACCCATTTG TTGACCTGGC CATCACCATC TGTATTGTCT TAAATACTCT TTTCATGGCC ATGGAGCACT ATCCAATGAC GGACCATTTC AATAATGTGC TTACAGTAGG AAACTTGGTT TTCACTGGGA TCTTTACAGC AGAAATGTTT CTGAAAATTA TTGCCATGGA TCCTTACTAT TATTTCCAAG AAGGCTGGAA TATCTTTGAC GGTTTTATTG TGACGCTTAG CCTGGTAGAA CTTGGACTCG CCAATGTGGA AGGATTATCT GTTCTCCGTT CATTTCGATT GCTGCGAGTT TTCAAGTTGG CAAAATCTTG GCCAACGTTA AATATGCTAA TAAAGATCAT CGGCAATTCC GTGGGGGCTC TGGGAAATTT AACCCTCGTC TTGGCCATCA TCGTCTTCAT TTTTGCCGTG GTCGGCATGC AGCTCTTTGG TAAAAGCTAC AAAGATTGTG TCTGCAAGAT CGCCAGTGAT TGTCAACTCC CACGCTGGCA CATGAATGAC TTCTTCCACT CCTTCCTGAT TGTGTTCCGC GTGCTGTGTG GGGAGTGGAT AGAGACCATG TGGGACTGTA TGGAGGTTGC TGGTCAAGCC ATGTGCCTTA CTGTCTTCAT GATGGTCATG GTGATTGGAA ACCTAGTGGT CCTGAATCTC TTTCTGGCCT TGCTTCTGAG CTCATTTAGT GCAGACAACC TTGCAGCCAC TGATGATGAT AATGAAATGA ATAATCTCCA AATTGCTGTG GATAGGATGC ACAAAGGAGT AGCTTATGTG AAAAGAAAAA TATATGAATT TATTCAACAG TCCTTCATTA GGAAACAAAA GATTTTAGAT GAAATTAAAC CACTTGATGA TCTAAACAAC AAGAAAGACA GTTGTATGTC CAATCATACA GCAGAAATTG GGAAAGATCT TGACTATCTT AAAGATGTAA ATGGAACTAC AAGTGGTATA GGAACTGGCA GCAGTGTTGA AAAATACATT ATTGATGAAA GTGATTACAT GTCATTCATA AACAACCCCA GTCTTACTGT GACTGTACCA ATTGCTGTAG GAGAATCTGA CTTTGAAAAT TTAAACACGG AAGACTTTAG TAGTGAATCG GATCTGGAAG AAAGCAAAGA GAAACTGAAT GAAAGCAGTA GCTCATCAGA AGGTAGCACT GTGGACATCG GCGCACCTGT AGAAGAACAG CCCGTAGTGG AACCTGAAGA AACTCTTGAA CCAGAAGCTT GTTTCACTGA AGGCTGTGTA CAAAGATTCA AGTGTTGTCA AATCAATGTG GAAGAAGGCA GAGGAAAACA ATGGTGGAAC CTGAGAAGGA CGTGTTTCCG AATAGTTGAA CATAACTGGT TTGAGACCTT CATTGTTTTC ATGATTCTCC TTAGTAGTGG TGCTCTGGCA TTTGAAGATA TATATATTGA TCAGCGAAAG ACGATTAAGA CGATGTTGGA ATATGCTGAC AAGGTTTTCA CTTACATTTT CATTCTGGAA ATGCTTCTAA AATGGGTGGC ATATGGCTAT CAAACATATT TCACCAATGC CTGGTGTTGG CTGGACTTCT TAATTGTTGA TGTTTCATTG GTCAGTTTAA CAGCAAATGC CTTGGGTTAC TCAGAACTTG GAGCCATCAA ATCTCTCAGG ACACTAAGAG CTCTGAGACC TCTAAGAGCC TTATCTCGAT TTGAAGGGAT GAGGGTGGTT GTGAATGCCC TTTTAGGAGC AATTCCATCC ATCATGAATG TGCTTCTGGT TTGTCTTATA TTCTGGCTAA TTTTCAGCAT CATGGGCGTA AATTTGTTTG CTGGCAAATT CTACCACTGT ATTAACACCA CAACTGGTGA CAGGTTTGAC ATCGAAGACG TGAATAATCA TACTGATTGC CTAAAACTAA TAGAAAGAAA TGAGACTGCT CGATGGAAAA ATGTGAAAGT AAACTTTGAT AATGTAGGAT TTGGGTATCT CTCTTTGCTT CAAGTTGCCA CATTCAAAGG ATGGATGGAT ATAATGTATG CAGCAGTTGA TTCCAGAAAT GTGGAACTCC AGCCTAAGTA TGAAGAAAGT CTGTACATGT ATCTTTACTT TGTTATTTTC ATCATCTTTG GGTCCTTCTT CACCTTGAAC CTGTTTATTG GTGTCATCAT AGATAATTTC AACCAGCAGA AAAAGAAGTT TGGAGGTCAA GACATCTTTA TGACAGAAGA ACAGAAGAAA TACTATAATG CAATGAAAAA ATTAGGATCG AAAAAACCGC AAAAGCCTAT ACCTCGACCA GGAAACAAAT TTCAAGGAAT GGTCTTTGAC TTCGTAACCA GACAAGTTTT TGACATAAGC ATCATGATTC TCATCTGTCT TAACATGGTC ACAATGATGG TGGAAACAGA TGACCAGAGT GAATATGTGA CTACCATTTT GTCACGCATC AATCTGGTGT TCATTGTGCT ATTTACTGGA GAGTGTGTAC TGAAACTCAT CTCTCTACGC CATTATTATT TTACCATTGG ATGGAATATT TTTGATTTTG TGGTTGTCAT TCTCTCCATT GTAGGTATGT TTCTTGCCGA GCTGATAGAA AAGTATTTCG TGTCCCCTAC CCTGTTCCGA GTGATCCGTC TTGCTAGGAT TGGCCGAATC CTACGTCTGA TCAAAGGAGC AAAGGGGATC CGCACGCTGC TCTTTGCTTT GATGATGTCC CTTCCTGCGT TGTTTAACAT CGGCCTCCTA CTCTTCCTAG TCATGTTCAT CTACGCCATC TTTGGGATGT CCAACTTTGC CTATGTTAAG AGGGAAGTTG GGATCGATGA CATGTTCAAC TTTGAGACCT TTGGCAACAG CATGATCTGC CTATTCCAAA TTACAACCTC TGCTGGCTGG GATGGATTGC TAGCACCCAT TCTCAACAGT AAGCCACCCG ACTGTGACCC TAATAAAGTT AACCCTGGAA GCTCAGTTAA GGGAGACTGT GGGAACCCAT CTGTTGGAAT TTTCTTTTTT GTCAGTTACA TCATCATATC CTTCCTGGTT GTGGTGAACA TGTACATCGC GGTCATCCTG GAGAACTTCA GTGTTGCTAC TGAAGAAAGT GCAGAGCCTC TGAGTGAGGA TGACTTTGAG ATGTTCTATG AGGTTTGGGA GAAGTTTGAT CCCGATGCAA CTCAGTTCAT GGAATTTGAA AAATTATCTC AGTTTGCAGC TGCGCTTGAA CCGCCTCTCA ATCTGCCACA ACCAAACAAA CTCCAGCTCA TTGCCATGGA TTTGCCCATG GTGAGTGGTG ACCGGATCCA CTGTCTTGAT ATCTTATTTG CTTTTACAAA GCGGGTTCTA GGAGAGAGTG GAGAGATGGA TGCTCTACGA ATACAGATGG AAGAGCGATT CATGGCTTCC AATCCTTCCA AGGTCTCCTA TCAGCCAATC ACTACTACTT TAAAACGAAA ACAAGAGGAA GTATCTGCTG TCATTATTCA GCGTGCTTAC AGACGCCACC TTTTAAAGCG AACTGTAAAA CAAGCTTCCT TTACGTACAA TAAAAACAAA ATCAAAGGTG GGGCTAATCT TCTTATAAAA GAAGACATGA TAATTGACAG AATAAATGAA AACTCTATTA CAGAAAAAAC TGATCTGACC ATGTCCACTG CAGCTTGTCC ACCTTCCTAT GACCGGGTGA CAAAGCCAAT TGTGGAAAAA CATGAGCAAG AAGGCAAAGA TGAAAAAGCC AAAGGGAAAC TCGAGATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAGGGGCC CGATATCTCG ACAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAGCT GACGTCCTTT CCATGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG CCTGGAAACG GGGGAGGCTA ACTGAAACAC GGAAGGAGAC AATACCGGAA GGAACCCGCG CTATGACGGC AATAAAAAGA CAGAATAAAA CGCACGGGTG TTGGGTCGTT TGTTCATAAA CGCGGGGTTC GGTCCCAGGG CTGGCACTCT GTCGATACCC CACCGAGACC CCATTGGGGC CAATACGCCC GCGTTTCTTC CTTTTCCCCA CCCCACCCCC CAAGTTCGGG TGAAGGCCCA GGGCTCGCAG CCAACGTCGG GGCGGCAGGC CCTGCCATAG CAGATCTGCG CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGCATCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGGGG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC AATGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCGACGGATC GGGAGATCTC CCGATCCCCT ATGGTCGACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATCTGCTCCC TGCTTGTGTG TTGGAGGTCG CTGAGTAGTG CGCGAGCAAA ATTTAAGCTA CAACAAGGCA AGGCTTGACC GACAATTGCA TGAAGAATCT GCTTAGG
