pLV2-Tight-CHD1(human)
$128.00
In stock
SKU
PL38195
NM_001270.4;CHD-1; PILBOS
100μg liquid plasmid(Instantly):$149
| name | pLV2-Tight-CHD1(human) | another name | apLV2-Tight-CHD1(human) |
| Insert Size (bp) | 5133bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression,Tetracycline induction | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | TRE | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGGAAGGGCT AATTCACTCC CAAAGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA CACAAGGCTA CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA GAAGAGGCCA ATAAAGGAGA GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG AGAGAGAAGT GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC GTGGCCCGAG AGCTGCATCC GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG GGACTTTCCG CTGGGGACTT TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA GCCCTCAGAT CCTGCATATA AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGCCTTT AAAAGAAAAG GGGGGATTGG GGGGTACAGT GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC AAACTAAAGA ACTACAAAAA CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG ACAGCAGAGA TCCAGTTTAT CGATGAGGCC CTTTCGTCTT CACTCGAGTT TACTCCCTAT CAGTGATAGA GAACGTATGT CGAGTTTACT CCCTATCAGT GATAGAGAAC GATGTCGAGT TTACTCCCTA TCAGTGATAG AGAACGTATG TCGAGTTTAC TCCCTATCAG TGATAGAGAA CGTATGTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGTCGAGTTT ATCCCTATCA GTGATAGAGA ACGTATGTCG AGTTTACTCC CTATCAGTGA TAGAGAACGT ATGTCGAGGT AGGCGTGTAC GGTGGGAGGC CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGATCGCC TGGAGAAGGA TCCGCGGCCG CCACCATGAA TGGACACAGT GATGAAGAAA GTGTTAGAAA CAGTAGTGGA GAATCAAGCC AGTCGGATGA TGATTCTGGG TCAGCTTCAG GCTCTGGATC TGGTTCGAGT TCTGGAAGCA GTAGTGATGG AAGCAGTAGC CAGTCAGGTA GCAGTGACTC TGACTCCGGA TCTGAATCAG GCAGTCAGTC AGAGTCTGAG TCAGACACTT CCCGAGAAAA CAAAGTTCAA GCAAAACCAC CGAAAGTTGA TGGAGCTGAG TTTTGGAAAT CTAGTCCTAG TATTCTGGCC GTTCAGAGAT CTGCAATCCT CAAGAAGCAG CAACAGCAGC AGCAGCAACA ACAACATCAA GCCTCATCTA ATAGCGGATC AGAAGAGGAT TCCTCTAGCA GTGAAGATTC CGATGACTCA TCAAGTGAGG TCAAAAGGAA AAAGCATAAA GATGAAGATT GGCAAATGTC TGGGTCAGGA TCTCCATCTC AGTCTGGTTC AGATTCAGAA TCTGAAGAAG AGAGAGAGAA AAGCAGTTGT GATGAAACAG AATCTGATTA TGAGCCAAAA AACAAAGTCA AAAGCAGAAA ACCTCAAAAT AGATCTAAGT CAAAAAATGG AAAGAAGATT CTTGGACAAA AAAAGAGACA GATTGATTCA TCTGAGGAGG ATGATGATGA AGAAGATTAT GATAATGATA AAAGAAGTTC TCGTCGCCAA GCAACTGTTA ATGTTAGCTA TAAGGAGGAT GAAGAAATGA AAACAGATTC TGATGACCTA CTGGAAGTCT GTGGAGAGGA TGTTCCTCAA CCTGAGGAAG AGGAATTTGA AACCATAGAA AGATTTATGG ATTGTCGGAT TGGGAGAAAA GGAGCTACTG GTGCTACTAC AACCATCTAT GCAGTTGAAG CAGATGGTGA CCCAAATGCA GGCTTTGAAA AAAACAAAGA ACCAGGAGAG ATTCAGTATT TAATTAAATG GAAAGGATGG TCCCATATCC ACAACACTTG GGAGACAGAA GAAACCCTCA AGCAGCAGAA TGTTAGAGGA ATGAAAAAAT TGGATAATTA TAAGAAAAAA GATCAGGAAA CAAAAAGATG GTTGAAAAAT GCCTCTCCAG AAGATGTGGA ATATTATAAT TGCCAGCAAG AACTTACAGA TGATCTACAT AAACAGTATC AAATAGTGGA ACGTATAATT GCTCATTCCA ATCAAAAGTC AGCAGCTGGT TATCCTGATT ATTACTGCAA ATGGCAGGGC CTTCCATACT CAGAGTGCAG CTGGGAAGAT GGAGCTCTCA TTTCCAAAAA GTTTCAAGCA TGCATTGATG AGTATTTTAG CAGGAACCAA TCAAAAACCA CTCCTTTTAA AGATTGCAAA GTATTAAAAC AAAGGCCAAG GTTTGTAGCC CTGAAGAAGC AGCCATCCTA TATTGGAGGA CATGAGGGCT TAGAATTAAG AGATTATCAA CTGAATGGTT TAAATTGGCT TGCTCATTCT TGGTGCAAAG GAAATAGTTG CATACTCGCT GATGAAATGG GCCTTGGAAA AACAATACAG ACGATCTCAT TTCTGAATTA TTTGTTTCAT GAACATCAAT TATATGGACC TTTTTTATTG GTAGTACCGC TCTCCACTCT TACTTCCTGG CAAAGGGAAA TTCAGACTTG GGCTTCTCAA ATGAATGCTG TGGTTTATTT AGGTGACATT AACAGCAGAA ACATGATAAG AACTCATGAA TGGACGCATC ATCAGACCAA ACGGTTAAAA TTTAATATAT TGTTAACAAC TTATGAAATT TTATTAAAAG ATAAGGCATT CCTTGGAGGT CTAAATTGGG CATTTATAGG TGTTGATGAA GCACACCGAT TAAAGAATGA TGACTCCCTT CTGTATAAAA CTTTAATAGA TTTTAAATCC AATCATCGTC TCCTTATCAC TGGAACTCCT CTACAGAATT CCCTCAAAGA GCTCTGGTCT TTGCTACATT TCATTATGCC AGAAAAGTTT TCTTCCTGGG AAGATTTTGA AGAAGAACAT GGCAAAGGGA GAGAATATGG TTATGCAAGC CTTCACAAGG AGCTTGAGCC ATTTCTGTTA CGCCGAGTTA AGAAAGATGT GGAAAAATCT CTTCCTGCCA AGGTTGAGCA GATTTTAAGA ATGGAAATGA GTGCTTTACA GAAACAATAT TACAAATGGA TTTTAACTAG GAATTACAAA GCCCTCAGCA AAGGTTCCAA GGGCAGTACC TCAGGCTTTT TGAACATTAT GATGGAGCTA AAGAAATGTT GTAACCATTG CTACCTCATT AAACCACCAG ATAATAATGA ATTCTATAAT AAACAGGAGG CCTTACAACA CTTAATTCGT AGTAGCGGAA AATTGATTCT TCTTGACAAG CTATTAATTC GCCTAAGAGA ACGAGGCAAT CGAGTTCTTA TTTTTTCACA AATGGTGCGG ATGTTAGATA TACTTGCAGA ATATTTGAAA TATCGTCAAT TCCCCTTTCA AAGATTAGAT GGATCAATAA AAGGAGAACT GAGGAAACAA GCTCTAGATC ATTTTAATGC TGAGGGATCA GAGGATTTTT GCTTTTTGCT GTCCACAAGA GCTGGAGGTC TAGGGATTAA TTTAGCCTCT GCTGACACTG TTGTTATATT TGATTCCGAT TGGAATCCAC AGAATGATCT TCAGGCACAG GCTAGAGCCC ATCGAATTGG GCAAAAGAAA CAGGTGAATA TTTATCGTCT AGTTACAAAG GGATCAGTTG AAGAAGATAT TCTTGAAAGG GCGAAAAAGA AGATGGTTTT AGATCATCTT GTAATTCAAA GAATGGACAC AACTGGGAAG ACAGTACTAC ATACAGGTTC TGCCCCATCA AGTTCTACTC CTTTCAATAA AGAAGAGTTA TCAGCCATTT TAAAGTTTGG TGCTGAAGAA CTTTTTAAGG AACCTGAAGG AGAAGAACAA GAGCCCCAGG AAATGGATAT AGATGAAATC TTGAAGAGAG CTGAAACTCA TGAAAATGAA CCAGGTCCTT TAACTGTAGG AGATGAATTG CTTTCCCAGT TCAAGGTTGC CAACTTCTCA AATATGGATG AGGATGACAT TGAGTTGGAA CCTGAAAGAA ATTCAAAGAA TTGGGAGGAA ATTATTCCAG AAGATCAAAG AAGACGATTA GAAGAAGAAG AAAGACAAAA GGAACTTGAA GAAATTTATA TGCTCCCAAG AATGAGAAAT TGTGCAAAAC AGATTAGTTT CAATGGAAGT GAAGGGAGGC GCAGTAGAAG TAGGAGATAC TCTGGATCTG ATAGTGATTC CATCTCAGAA GGGAAAAGGC CAAAGAAACG TGGAAGACCA CGGACTATTC CTCGGGAGAA TATTAAAGGA TTTAGTGATG CAGAAATTAG GCGGTTTATC AAGAGCTATA AGAAATTTGG TGGTCCTCTG GAAAGATTAG ATGCAATTGC TCGAGATGCT GAGTTAGTTG ATAAGTCAGA AACAGACCTT AGACGACTGG GAGAATTGGT ACATAATGGT TGCATTAAAG CATTAAAGGA TAGTTCTTCA GGAACAGAAC GAACAGGTGG TAGACTCGGA AAAGTGAAGG GTCCAACATT CCGAATATCA GGAGTACAGG TGAATGCCAA ACTAGTCATC TCCCATGAAG AAGAATTAAT ACCTTTGCAC AAATCCATTC CTTCTGATCC AGAAGAAAGA AAGCAGTATA CTATCCCATG CCACACAAAG GCAGCTCATT TTGATATAGA CTGGGGCAAA GAAGATGATT CCAATTTGTT AATTGGCATC TATGAATATG GATATGGAAG CTGGGAAATG ATTAAAATGG ATCCTGACCT CAGTCTAACA CACAAGATTC TTCCAGATGA TCCCGATAAA AAACCACAAG CAAAACAGTT GCAGACCCGT GCAGACTACC TCATCAAATT ACTTAGTAGA GATCTTGCAA AAAAAGAAGC TCTTTCTGGT GCGGGAAGTT CAAAGAGGAG AAAAGCAAGA GCTAAGAAGA ATAAAGCAAT GAAGTCTATA AAAGTGAAAG AGGAAATAAA GAGTGATTCT TCTCCTCTGC CTTCAGAGAA GTCTGATGAA GATGATGATA AATTGAGTGA ATCCAAGTCT GATGGTAGGG AAAGATCCAA GAAATCTTCA GTGTCAGATG CTCCAGTTCA TATCACGGCA AGTGGTGAAC CAGTTCCCAT TTCTGAAGAA TCTGAAGAGC TGGATCAGAA GACATTCAGC ATTTGTAAAG AAAGAATGAG GCCTGTTAAA GCAGCTTTGA AACAACTTGA TAGGCCTGAG AAAGGCCTTT CAGAAAGAGA ACAACTAGAG CATACTAGAC AATGTTTAAT AAAAATTGGA GACCATATCA CAGAATGTCT AAAAGAGTAT ACAAATCCTG AACAAATTAA GCAATGGAGA AAAAACCTGT GGATTTTTGT ATCTAAGTTT ACTGAATTTG ATGCAAGAAA ATTACATAAA TTATATAAGC ATGCTATTAA AAAACGGCAG GAGTCTCAGC AAAACAGTGA TCAAAACAGC AACTTGAATC CTCACGTGAT TAGAAATCCA GATGTGGAAA GATTAAAAGA GAATACAAAT CACGATGATA GCAGCAGGGA CAGTTATTCC TCTGATAGAC ACTTAACTCA GTACCATGAT CATCATAAAG ACCGACATCA GGGAGATTCT TACAAAAAAA GTGATTCCAG GAAAAGACCC TATTCTTCTT TTAGTAATGG TAAAGACCAT CGTGATTGGG ATCACTACAA GCAAGACAGC AGATATTACA GTGACAGAGA GAAACACAGA AAACTGGATG ATCACAGGAG TAGAGATCAC AGGTCAAATT TGGAAGGAAG TTTAAAAGAT AGATCTCATT CTGATCATCG TTCTCACTCA GATCATCGGT TACATTCAGA CCACCGGTCA AGTTCTGAAT ATACGCACCA TAAATCTTCC AGGGATTATA GGTATCACTC AGACTGGCAA ATGGACCACA GAGCTTCCAG CAGTGGCCCT AGGTCACCAC TAGATCAGAG ATCTCCTTAT GGCTCCAGAT CTCCATTTGA ACATTCAGTT GAACACAAAA GTACACCGGA GCATACCTGG AGTAGTCGGA AAACATAAAC GCGTGAATTC CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAGCTGACGT CCTTTCCATG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCCTGG AAGTTTAAAC CTGCAGTCGA GACCTAGAAA AACATGGAGC AATCACAAGT AGCAATACAG CAGCTACCAA TGCTGATTGT GCCTGGCTAG AAGCACAAGA GGAGGAGGAG GTGGGTTTTT CCAGTCACAC CTCAGGTACC TTTAAGACCA ATGACTTACA AGGCAGCTGT AGATCTTAGC CACTTTTTAA AAGAAAAGAG GGGACTGCTG GAAGGGCTAA TTCACTCCCA ACGAAGACAA GATATCCTTG ATCTGTGGAT CTACCACACA CAAGGCTACT TCCCTGATTA GCAGAACTAC ACACCAGGGC CAGGGGTCAG ATATCCACTG ACCTTTGGAT GGTGCTACAA GCTAGTACCA GTTGAGCCAG ATAAGGTAGA AGAGGCCAAT AAAGGAGAGA ACACCAGCTT GTTACACCCT GTGAGCCTGC ATGGGATGGA TGACCCGGAG AGAGAAGTGT TAGAGTGGAG GTTTGACAGC CGCCTAGCAT TTCATCACGT GGCCCGAGAG CTGCATCCGG AGTACTTCAA GAACTGCTGA TATCGAGCTT GCTACAAGGG ACTTTCCGCT GGGGACTTTC CAGGGAGGCG TGGCCTGGGC GGGACTGGGG AGTGGCGAGC CCTCAGATCC TGCATATAAG CAGCTGCTTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGTAGTAGT TCATGTCATC TTATTATTCA GTATTTATAA CTTGCAAAGA AATGAATATC AGAGAGTGAG AGGCCTTGAC ATTGCTAGCG TTTTACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCGACGG ATCGGGAGAT CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGATCAACT GGATAACTCA AGCTAACCAA AATCATCCCA AACTTCCCAC CCCATACCCT ATTACCACTG CCAATTACCT AGTGGTTTCA TTTACTCTAA ACCTGTGATT CCTCTGAATT ATTTTCATTT TAAAGAAATT GTATTTGTTA AATATGTACT ACAAACTTAG TAGTTTTTAA AGAAATTGTA TTTGTTAAAT ATGTACTACA AACTTAGTAG T
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