pLac-6×His-T7 RNA polymerase
$84.00
In stock
SKU
PL38180
100μg liquid plasmid(Instantly):$105
| name | pLac-6×His-T7 RNA polymerase | another name | apLac-6×His-T7 RNA polymerase |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | protease | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | Lac | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCTT GCATGCCTGC AGGTCGACTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGGCAGCAG CCATCATCAT CATCATCACA GCAGCGGCGA TGACGACGAC AAGATGAACA CGATTAACAT CGCTAAGAAC GACTTCTCTG ACATCGAACT GGCTGCTATC CCGTTCAACA CTCTGGCTGA CCATTACGGT GAGCGTTTAG CTCGCGAACA GTTGGCCCTT GAGCATGAGT CTTACGAGAT GGGTGAAGCA CGCTTCCGCA AGATGTTTGA GCGTCAACTT AAAGCTGGTG AGGTTGCGGA TAACGCTGCC GCCAAGCCTC TCATCACTAC CCTACTCCCT AAGATGATTG CACGCATCAA CGACTGGTTT GAGGAAGTGA AAGCTAAGCG CGGCAAGCGC CCGACAGCCT TCCAGTTCCT GCAAGAAATC AAGCCGGAAG CCGTAGCGTA CATCACCATT AAGACCACTC TGGCTTGCCT AACCAGTGCT GACAATACAA CCGTTCAGGC TGTAGCAAGC GCAATCGGTC GGGCCATTGA GGACGAGGCT CGCTTCGGTC GTATCCGTGA CCTTGAAGCT AAGCACTTCA AGAAAAACGT TGAGGAACAA CTCAACAAGC GCGTAGGGCA CGTCTACAAG AAAGCATTTA TGCAAGTTGT CGAGGCTGAC ATGCTCTCTA AGGGTCTACT CGGTGGCGAG GCGTGGTCTT CGTGGCATAA GGAAGACTCT ATTCATGTAG GAGTACGCTG CATCGAGATG CTCATTGAGT CAACCGGAAT GGTTAGCTTA CACCGCCAAA ATGCTGGCGT AGTAGGTCAA GACTCTGAGA CTATCGAACT CGCACCTGAA TACGCTGAGG CTATCGCAAC CCGTGCAGGT GCGCTGGCTG GCATCTCTCC GATGTTCCAA CCTTGCGTAG TTCCTCCTAA GCCGTGGACT GGCATTACTG GTGGTGGCTA TTGGGCTAAC GGTCGTCGTC CTCTGGCGCT GGTGCGTACT CACAGTAAGA AAGCACTGAT GCGCTACGAA GACGTTTACA TGCCTGAGGT GTACAAAGCG ATTAACATTG CGCAAAACAC CGCATGGAAA ATCAACAAGA AAGTCCTAGC GGTCGCCAAC GTAATCACCA AGTGGAAGCA TTGTCCGGTC GAGGACATCC CTGCGATTGA ACGTGAAGAA CTCCCGATGA AACCGGAAGA CATCGACATG AATCCTGAGG CTCTCACCGC GTGGAAACGT GCTGCCGCTG CTGTGTACCG CAAGGACAAG GCTCGCAAGT CTCGCCGTAT CAGCCTTGAG TTCATGCTTG AGCAAGCCAA TAAGTTTGCT AACCATAAGG CCATCTGGTT CCCTTACAAC ATGGACTGGC GCGGTCGTGT TTACGCTGTG TCAATGTTCA ACCCGCAAGG TAACGATATG ACCAAAGGAC TGCTTACGCT GGCGAAAGGT AAACCAATCG GTAAGGAAGG TTACTACTGG CTGAAAATCC ACGGTGCAAA CTGTGCGGGT GTCGATAAGG TTCCGTTCCC TGAGCGCATC AAGTTCATTG AGGAAAACCA CGAGAACATC ATGGCTTGCG CTAAGTCTCC ACTGGAGAAC ACTTGGTGGG CTGAGCAAGA TTCTCCGTTC TGCTTCCTTG CGTTCTGCTT TGAGTACGCT GGGGTACAGC ACCACGGCCT GAGCTATAAC TGCTCCCTTC CGCTGGCGTT TGACGGGTCT TGCTCTGGCA TCCAGCACTT CTCCGCGATG CTCCGAGATG AGGTAGGTGG TCGCGCGGTT AACTTGCTTC CTAGTGAAAC CGTTCAGGAC ATCTACGGGA TTGTTGCTAA GAAAGTCAAC GAGATTCTAC AAGCAGACGC AATCAATGGG ACCGATAACG AAGTAGTTAC CGTGACCGAT GAGAACACTG GTGAAATCTC TGAGAAAGTC AAGCTGGGCA CTAAGGCACT GGCTGGTCAA TGGCTGGCTT ACGGTGTTAC TCGCAGTGTG ACTAAGCGTT CAGTCATGAC GCTGGCTTAC GGGTCCAAAG AGTTCGGCTT CCGTCAACAA GTGCTGGAAG ATACCATTCA GCCAGCTATT GATTCCGGCA AGGGTCTGAT GTTCACTCAG CCGAATCAGG CTGCTGGATA CATGGCTAAG CTGATTTGGG AATCTGTGAG CGTGACGGTG GTAGCTGCGG TTGAAGCAAT GAACTGGCTT AAGTCTGCTG CTAAGCTGCT GGCTGCTGAG GTCAAAGATA AGAAGACTGG AGAGATTCTT CGCAAGCGTT GCGCTGTGCA TTGGGTAACT CCTGATGGTT TCCCTGTGTG GCAGGAATAC AAGAAGCCTA TTCAGACGCG CTTGAACCTG ATGTTCCTCG GTCAGTTCCG CTTACAGCCT ACCATTAACA CCAACAAAGA TAGCGAGATT GATGCACACA AACAGGAGTC TGGTATCGCT CCTAACTTTG TACACAGCCA AGACGGTAGC CACCTTCGTA AGACTGTAGT GTGGGCACAC GAGAAGTACG GAATCGAATC TTTTGCACTG ATTCACGACT CCTTCGGTAC CATTCCGGCT GACGCTGCGA ACCTGTTCAA AGCAGTGCGC GAAACTATGG TTGACACATA TGAGTCTTGT GATGTACTGG CTGATTTCTA CGACCAGTTC GCTGACCAGT TGCACGAGTC TCAATTGGAC AAAATGCCAG CACTTCCGGC TAAAGGTAAC TTGAACCTCC GTGACATCTT AGAGTCGGAC TTCGCGTTCG CGTAACGCCA AATCAATACG ACTCCGGATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGTGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAA
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
