pLV2-Tight-INO80(human)
$113.00
In stock
SKU
PL38148
NM_017553.3;INO80A; INOC1
100μg liquid plasmid(Instantly):$134
| name | pLV2-Tight-INO80(human) | another name | apLV2-Tight-INO80(human) |
| Insert Size (bp) | 4671bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression,Tetracycline induction | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | TRE | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGGAAGGGCT AATTCACTCC CAAAGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA CACAAGGCTA CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA GAAGAGGCCA ATAAAGGAGA GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG AGAGAGAAGT GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC GTGGCCCGAG AGCTGCATCC GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG GGACTTTCCG CTGGGGACTT TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA GCCCTCAGAT CCTGCATATA AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGCCTTT AAAAGAAAAG GGGGGATTGG GGGGTACAGT GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC AAACTAAAGA ACTACAAAAA CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG ACAGCAGAGA TCCAGTTTAT CGATGAGGCC CTTTCGTCTT CACTCGAGTT TACTCCCTAT CAGTGATAGA GAACGTATGT CGAGTTTACT CCCTATCAGT GATAGAGAAC GATGTCGAGT TTACTCCCTA TCAGTGATAG AGAACGTATG TCGAGTTTAC TCCCTATCAG TGATAGAGAA CGTATGTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGTCGAGTTT ATCCCTATCA GTGATAGAGA ACGTATGTCG AGTTTACTCC CTATCAGTGA TAGAGAACGT ATGTCGAGGT AGGCGTGTAC GGTGGGAGGC CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGATCGCC TGGAGAAGGA TCCGCGGCCG CCACCATGGC CTCGGAGTTG GGTGCCAGGG ATGATGGAGG CTGCACTGAG CTGGCAAAGC CCCTCTATCT TCAGTACTTG GAGAGGGCCC TCCGGTTGGA CCATTTTCTG CGACAAACGT CAGCTATCTT CAATAGGAAT ATTTCTAGTG ATGACAGTGA AGATGGACTG GATGACAGTA ATCCATTATT GCCCCAGTCT GGGGATCCCT TAATACAAGT TAAGGAAGAA CCTCCAAATT CATTGCTTGG TGAAACTTCT GGAGCAGGCA GTTCTGGAAT GTTAAACACA TATTCTCTGA ATGGAGTTCT ACAGTCAGAA TCAAAATGTG ATAAGGGGAA TTTATATAAT TTCTCTAAGC TGAAGAAAAG CAGAAAGTGG CTAAAGAGCA TTCTGCTAAG TGATGAATCC AGCGAGGCTG ATTCTCAGAG TGAAGACGAT GATGAAGAAG AACTCAATCT CAGCAGAGAA GAACTTCACA ACATGCTTCG ACTACACAAA TATAAGAAAC TTCACCAAAA TAAGTATAGT AAAGACAAGG AGTTGCAGCA ATATCAGTAC TACAGTGCAG GCCTGCTCTC CACATATGAC CCTTTCTATG AGCAACAACG GCACCTACTT GGACCCAAGA AAAAGAAATT TAAGGAGGAA AAGAAACTTA AAGCTAAGTT GAAAAAAGTG AAGAAAAAAA GACGAAGAGA TGAAGAACTT TCCTCTGAAG AATCCCCTCG TCGCCATCAC CACCAGACCA AAGTCTTTGC CAAGTTTTCT CACGATGCAC CTCCCCCTGG CACTAAGAAA AAGCACTTAT CCATTGAGCA GCTGAATGCT CGTCGCAGGA AAGTATGGCT CAGCATTGTG AAAAAGGAAC TACCAAAGGC AAATAAGCAG AAAGCTTCAG CTCGTAACCT GTTTCTCACC AATAGCCGAA AGCTTGCTCA CCAGTGCATG AAGGAGGTGC GTCGAGCTGC CTTGCAGGCC CAGAAGAACT GTAAGGAAAC CTTGCCTCGT GCCCGCCGCC TCACCAAGGA GATGCTTCTG TACTGGAAGA AATATGAGAA AGTAGAGAAG GAGCACCGCA AGAGAGCAGA GAAGGAAGCT TTGGAGCAGC GGAAGTTGGA TGAGGAAATG CGGGAGGCCA AGAGGCAACA GCGAAAACTC AACTTCTTAA TTACCCAGAC AGAGTTGTAT GCCCATTTCA TGAGTCGCAA ACGAGATATG GGTCATGATG GTATCCAGGA AGAAATCCTA AGGAAACTGG AAGACAGTTC TACCCAGAGA CAAATCGATA TAGGTGGAGG AGTGGTAGTT AACATCACAC AGGAGGATTA TGATAGTAAC CATTTTAAAG CCCAGGCCCT GAAGAATGCT GAAAATGCTT ACCATATTCA CCAAGCTCGG ACAAGGTCAT TTGATGAAGA TGCAAAAGAA AGTCGAGCAG CTGCCCTACG GGCAGCAAAC AAGTCTGGCA CTGGGTTTGG GGAGAGTTAT AGCCTGGCTA ACCCATCTAT CCGGGCTGGT GAGGATATTC CACAGCCCAC AATTTTTAAT GGCAAATTGA AAGGTTATCA ACTGAAAGGC ATGAATTGGT TGGCCAATCT ATATGAACAG GGTATTAATG GCATTCTTGC TGATGAAATG GGCCTTGGTA AAACAGTACA GAGCATTGCC CTTCTGGCCC ATCTGGCTGA GAGAGAGAAC ATTTGGGGAC CTTTCTTAAT AATTTCACCT GCGTCTACAC TTAACAATTG GCACCAGGAG TTTACTAGAT TTGTTCCTAA ATTTAAGGTG CTACCATATT GGGGAAATCC TCATGATAGA AAAGTCATCA GAAGGTTCTG GAGTCAGAAG ACCCTCTACA CTCAGGATGC CCCCTTCCAT GTGGTTATTA CCAGCTATCA GCTGGTGGTG CAGGATGTAA AGTATTTCCA GCGGGTCAAG TGGCAATACA TGGTACTGGA TGAGGCTCAG GCGCTCAAGA GTAGTTCCAG TGTTCGTTGG AAGATCCTCT TACAGTTCCA GTGTCGGAAT CGGCTTTTGC TAACCGGGAC CCCAATTCAG AACACCATGG CAGAGCTTTG GGCTCTGCTG CATTTCATTA TGCCAACATT ATTTGATTCA CATGAGGAAT TTAATGAATG GTTTTCCAAG GACATTGAGA GCCATGCCGA AAACAAATCT GCTATTGATG AGAATCAACT TTCTCGCTTA CACATGATTT TGAAGCCATT TATGCTGAGG AGAATCAAGA AAGATGTGGA AAATGAATTA TCTGACAAGA TTGAGATTCT AATGTATTGC CAACTGACCA GCCGACAGAA GCTGCTATAT CAGGCACTAA AGAACAAAAT TTCCATTGAG GATTTATTGC AGTCTTCTAT GGGCTCTACC CAACAAGCAC AGAACACCAC CAGCAGCCTC ATGAATCTGG TCATGCAGTT TAGGAAGGTG TGTAATCACC CGGAGTTATT TGAACGGCAA GAAACTTGGT CTCCATTTCA TATTTCCCTA AAGCCATACC ACATTTCAAA GTTTATCTAC CGTCATGGAC AGATCAGGGT CTTCAATCAT TCACGAGACA GGTGGTTAAG GGTTCTTTCT CCATTTGCAC CAGACTATAT CCAACGGTCT CTCTTTCACA GAAAAGGTAT TAATGAAGAA AGCTGTTTCT CTTTCCTTCG CTTTATTGAT ATATCTCCAG CAGAAATGGC AAACCTTATG CTTCAGGGAC TTTTGGCCAG ATGGTTAGCT CTTTTCCTGT CTCTGAAAGC CTCCTACAGG CTCCATCAGC TACGCTCCTG GGGAGCGCCA GAAGGGGAGA GCCACCAGAG ATACCTGAGG AACAAGGATT TCCTTCTTGG GGTTAATTTT CCACTCTCCT TTCCAAACCT TTGCAGCTGC CCTTTGTTAA AGTCTCTTGT TTTCAGCAGC CACTGTAAAG CAGTGAGTGG CTACTCAGAC CAGGTTGTCC ATCAGCGGAG ATCAGCTACC TCCTCGCTGC GTCGCTGCCT GCTCACTGAG CTGCCATCTT TTTTGTGTGT GGCCAGTCCA CGAGTTACCG CAGTGCCATT GGATTCTTAC TGCAATGACC GAAGTGCAGA ATATGAAAGG CGAGTTCTGA AGGAAGGAGG GAGTCTGGCA GCCAAGCAGT GTTTGTTGAA TGGGGCCCCT GAACTGGCTG CAGACTGGCT AAATAGACGA TCACAGTTCT TCCCAGAGCC AGCTGGAGGT CTGTGGAGCA TCAGACCTCA GAATGGCTGG TCTTTCATCA GGATTCCAGG CAAGGAGAGC CTCATCACTG ACAGTGGAAA GCTGTATGCC CTTGATGTCC TGCTGACTCG GCTCAAGTCT CAAGGGCATA GGGTCCTTAT CTACTCCCAG ATGACCAGGA TGATAGACCT ACTGGAGGAA TACATGGTTT ACAGGAAGCA TACCTACATG AGGCTTGATG GCTCATCCAA GATCTCGGAG AGGCGAGACA TGGTTGCTGA TTTTCAGAAC AGGAATGACA TCTTTGTGTT CCTGTTAAGC ACACGAGCTG GAGGACTGGG TATCAATCTC ACTGCTGCAG ACACAGTGAT TTTCTATGAT AGCGACTGGA ACCCCACTGT GGACCAGCAG GCCATGGACA GGGCCCACCG CTTAGGGCAG ACAAAGCAGG TTACTGTGTA CCGGCTCATC TGTAAAGGCA CCATTGAAGA ACGCATTCTG CAAAGAGCCA AGGAGAAGAG TGAGATTCAG CGGATGGTGA TTTCAGGTGG GAACTTCAAA CCAGATACCT TGAAACCCAA AGAGGTGGTT AGTCTTCTTC TAGACGACGA AGAGTTGGAG AAGAAACTGA GGCTGCGGCA GGAAGAGAAA CGGCAACAGG AGGAAACCAA CCGAGTGAAA GAGCGCAAGC GGAAGCGGGA AAAGTATGCA GAGAAGAAGA AAAAAGAAGA TGAATTGGAT GGGAAAAGGA GAAAAGAGGG TGTGAACCTG GTGATCCCAT TTGTTCCCTC GGCTGATAAC TCCAACCTCT CTGCTGACGG AGATGACTCC TTCATTAGCG TGGACTCAGC CATGCCAAGC CCTTTCAGTG AGATCTCCAT CAGCAGTGAG CTGCACACTG GCTCCATTCC CCTGGACGAG AGCAGCAGTG ACATGCTGGT CATTGTGGAT GACCCAGCCT CCTCAGCCCC TCAGTCTCGA GCTACCAACT CTCCCGCATC CATAACAGGC TCCGTCTCAG ATACCGTGAA TGGAATTTCC ATTCAGGAAA TGCCAGCTGC AGGACGTGGT CACTCAGCCC GAAGCCGAGG CCGCCCCAAA GGTTCAGGAA GCACAGCCAA AGGAGCAGGG AAGGGCCGGA GCCGAAAGTC CACGGCAGGC AGTGCTGCTG CAATGGCAGG AGCCAAAGCC GGGGCTGCAG CGGCCTCTGC AGCTGCCTAT GCCGCATACG GGTACAACGT GTCTAAAGGA ATCTCCGCCA GCAGTCCTCT GCAGACATCC CTTGTTCGGC CTGCTGGCCT TGCTGACTTT GGACCTTCAA GCGCCTCTTC TCCTTTGAGT TCCCCTTTGA GCAAGGGAAA TAATGTTCCT GGGAATCCCA AGAACCTCCA CATGACCAGC AGCCTAGCCC CAGACTCTCT GGTCCGGAAA CAGGGCAAAG GCACCAACCC CTCTGGAGGA CGGTAAACGC GTGAATTCCA ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA GCTGACGTCC TTTCCATGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCCTGGAA GTTTAAACCT GCAGTCGAGA CCTAGAAAAA CATGGAGCAA TCACAAGTAG CAATACAGCA GCTACCAATG CTGATTGTGC CTGGCTAGAA GCACAAGAGG AGGAGGAGGT GGGTTTTTCC AGTCACACCT CAGGTACCTT TAAGACCAAT GACTTACAAG GCAGCTGTAG ATCTTAGCCA CTTTTTAAAA GAAAAGAGGG GACTGCTGGA AGGGCTAATT CACTCCCAAC GAAGACAAGA TATCCTTGAT CTGTGGATCT ACCACACACA AGGCTACTTC CCTGATTAGC AGAACTACAC ACCAGGGCCA GGGGTCAGAT ATCCACTGAC CTTTGGATGG TGCTACAAGC TAGTACCAGT TGAGCCAGAT AAGGTAGAAG AGGCCAATAA AGGAGAGAAC ACCAGCTTGT TACACCCTGT GAGCCTGCAT GGGATGGATG ACCCGGAGAG AGAAGTGTTA GAGTGGAGGT TTGACAGCCG CCTAGCATTT CATCACGTGG CCCGAGAGCT GCATCCGGAG TACTTCAAGA ACTGCTGATA TCGAGCTTGC TACAAGGGAC TTTCCGCTGG GGACTTTCCA GGGAGGCGTG GCCTGGGCGG GACTGGGGAG TGGCGAGCCC TCAGATCCTG CATATAAGCA GCTGCTTTTT GCCTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GTAGTAGTTC ATGTCATCTT ATTATTCAGT ATTTATAACT TGCAAAGAAA TGAATATCAG AGAGTGAGAG GCCTTGACAT TGCTAGCGTT TTACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCGACGGAT CGGGAGATCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GATCAACTGG ATAACTCAAG CTAACCAAAA TCATCCCAAA CTTCCCACCC CATACCCTAT TACCACTGCC AATTACCTAG TGGTTTCATT TACTCTAAAC CTGTGATTCC TCTGAATTAT TTTCATTTTA AAGAAATTGT ATTTGTTAAA TATGTACTAC AAACTTAGTA GTTTTTAAAG AAATTGTATT TGTTAAATAT GTACTACAAA CTTAGTAGT
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