pCMV-HUWE1(human)-3×HA-Neo
$243.00
In stock
SKU
PL37780
100μg liquid plasmid(Instantly):$265
| name | pCMV-HUWE1(human)-3×HA-Neo | another name | apCMV-HUWE1(human)-3×HA-Neo |
| Insert Size (bp) | 13071bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCATGAA AGTAGACAGG ACTAAACTGA AGAAGACACC TACTGAGGCT CCTGCAGACT GCAGAGCCTT AATAGACAAA CTCAAAGTTT GTAATGATGA GCAACTTCTC TTGGAACTGC AGCAGATCAA AACATGGAAC ATTGGAAAGT GCGAGTTATA TCACTGGGTG GACCTGTTGG ACCGCTTCGA TGGAATACTG GCAGATGCTG GACAGACAGT GGAGAATATG TCATGGATGC TCGTATGTGA TAGGCCAGAA AGAGAGCAAC TGAAAATGCT TCTCTTGGCT GTGTTGAACT TCACAGCCTT GCTCATTGAG TACAGCTTTT CCCGGCATCT GTACAGTTCC ATAGAGCATT TGACAACTTT ATTGGCTTCC TCTGATATGC AAGTGGTGCT GGCAGTCCTC AATCTCCTAT ATGTATTTAG CAAAAGATCA AACTACATCA CTCGTCTGGG ATCTGACAAG AGGACCCCGC TGCTAACTCG GCTACAACAT TTGGCAGAGA GCTGGGGTGG AAAGGAGAAT GGCTTTGGAC TTGCAGAATG TTGCAGAGAC TTGCATATGA TGAAATATCC ACCCAGTGCA ACTACACTAC ACTTTGAATT CTATGCAGAT CCTGGGGCCG AGGTCAAAAT TGAGAAAAGG ACAACTAGTA ACACACTACA TTATATTCAC ATAGAGCAAC TTGACAAGAT TTCAGAAAGC CCTTCTGAAA TCATGGAATC TCTTACCAAA ATGTACAGCA TTCCTAAGGA TAAGCAGATG CTGTTATTTA CACACATACG ACTGGCCCAT GGCTTTTCTA ATCACAGGAA GCGATTGCAG GCAGTTCAGG CCAGACTGCA TGCAATATCT ATATTAGTGT ATTCCAATGC CTTGCAGGAA TCAGCAAACA GTATCTTGTA TAATGGCTTG ATAGAGGAGT TGGTAGATGT CCTTCAGATA ACGGATAAGC AGCTTATGGA GATTAAAGCA GCTTCTTTAC GAACATTAAC ATCAATTGTC CACTTGGAGA GAACTCCCAA ACTCAGCAGT ATTATTGACT GTACTGGAAC TGCCTCCTAC CATGGATTTT TGCCAGTGCT TGTAAGGAAC TGTATCCAGG CCATGATTGA TCCTTCCATG GATCCATACC CTCACCAGTT TGCCACTGCT CTCTTCTCTT TTTTATACCA TCTGGCCAGC TACGATGCTG GTGGTGAAGC CTTGGTCTCC TGTGGAATGA TGGAAGCCTT ATTGAAGGTC ATAAAGTTTC TTGGCGATGA ACAGGACCAG ATAACATTTG TCACCAGAGC CGTCAGAGTG GTTGACCTTA TCACCAACCT GGATATGGCA GCTTTTCAAT CCCATAGTGG ACTTTCTATC TTCATTTATA GACTTGAGCA TGAAGTAGAT CTGTGCCGAA AAGAATGTCC GTTTGTGATC AAGCCAAAGA TCCAGAGACC CAATACTACA CAAGAAGGAG AGGAAATGGA AACTGATATG GATGGAGTCC AGTGTATTCC ACAACGAGCA GCACTTCTGA AATCCATGTT GAATTTCCTC AAGAAGGCCA TCCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGATGGTTCT CTGCCTACCT CCCTGAAACA CATCATCAGC AATGCAGAAT ACTATGGCCC ATCACTCTTC CTCCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA CTCCAGGACA ATGGATTGAC AGATGTCATG CTGCATGCAC TGCTTATCAA AGATGTTCCT GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC CGAGGTCTTC AGTCTTTTGT TCAGTGTCAG CCTTTTGAAC GCCTCTTCAA AGTTCTTCTG TCTCCAGATT ACCTCCCAGC CATGCGGAGG AGGAGAAGTT CTGATCCCCT TGGGGATACT GCATCCAACC TGGGGAGTGC TGTCGATGAG CTCATGAGAC ATCAGCCCAC CCTTAAAACA GATGCAACGA CTGCCATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGGGACCCC AAATACATCT GTCAGAAGCC ATCGATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCC CCAAGGTCTA ATCATGCCGC AGAAGAAGCC TCTAGTGAGG ATGAGGAGGA AGAGGAAGTA CAGGCCATGC AGAGCTTTAA TTCTACCCAG CAAAATGAAA CTGAGCCTAA TCAGCAGGTT GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT CAGAAAGGAC TGTTGCCTTT GGTTACCATT TTGGGTCTTC CCAATCTGCC CATTGACTTT CCCACATCTG CTGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA CATGAACCCA AAGTCCTTCA AGAGGGTCTC CTTCAGTTGG ACTCCATCCT CTCCTCCCTG GAGCCCTTAC ACCGCCCCAT TGAATCCCCT GGGGGCTCAG TGTTGTTGCG AGAACTGGCT TGCGCAGGCA ATGTTGCTGA TGCTACCCTC TCAGCCCAGG CCACACCTCT GCTGCATGCA CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT GAAATTCGTT CCATCTCCGT AAACCAGTGG GGCTCTCAAT TGGGTCTGAG TGTTTTGAGC AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTCCT CTCTCTGTGT ACCCCAAACA GCCTACCATC TGGGTGTGAA TTTGGCCAGG CAGATATGCA GAAACTGGTT CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT GGAGCAGCTG GAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTAGAT GGTGACACAT TGGCTCCCAT GGAGACAGAT GAACCTACTG CTTCAGACTC TAAGGGCAAA TCTAAAATCA CACCAGCAAT GGCTGCCAGA ATTAAGCAAA TCAAGCCTTT GTTATCAGCT TCCTCCAGAT TAGGCCGAGC ACTTGCTGAG CTATTTGGAC TTCTTGTTAA ACTTTGTGTG GGATCTCCTG TCCGCCAGAG AAGGAGCCAT CATGCTGCCA GCACCACTAC AGCACCGACA CCTGCCGCGC GATCAACAGC CTCAGCTCTC ACTAAGCTCT TGACTAAGGG GTTATCTTGG CAGCCCCCAC CATATACACC TACTCCCCGA TTCAGGCTGA CATTCTTCAT CTGTTCAGTT GGTTTCACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CCTACCACCT CATGCTGCAA AAATTTCTCT GCTCCGGAGG CCACAATGCT CTTTTTGAAA CTTTCAACTG GGCTCTGTCC ATGGGAGGTA AAGTTCCTGT TTCTGAGGGA TTGGAACACT CAGACTTGCC TGATGGCACA GGAGAATTCC TAGATGCCTG GCTTATGCTG GTGGAGAAGA TGGTGAATCC CACCACGGTG CTTGAATCTC CACATTCGCT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCCCAG TTCAGTGCAC TGCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAAAAC TTATGGAACC GGAAACCCCT GAAGGTATAT GGTGGACGAA TGGCTGAATC GATGCTGGCC ATTCTATGCC ACATCCTCCG AGGAGAACCT GTGATTCGAG AGAGACTAAG CAAGGAGAAG GAGGGGTCTC GAGGAGAAGA GGATACAGGG CAAGAGGAAG GTGGCTCCCG CCGGGAACCT CAAGTCAACC AGCAACAACT GCAACAGCTC ATGGACATGG GCTTCACAAG GGAACATGCA ATGGAGGCAC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TTTAACCCAC CCTCCTCCAA TCATGGGAGG AGTTGTTCGG GATCTCAGCA TGTCTGAAGA GGACCAGATG ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATTCCAA TGGATCAAAG GGCAGAGTCA CCTGAGGAAG TTGCTTGCCG GAAGGAGGAA GAGGAACGGA AAGCTCGGGA AAAGCAGGAG GAGGAAGAGG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ACCCGTTGGA ACAAGATGAG CTCCACACTT TCACAGATAC TATGTTGCCA GGCTGCTTCC ACCTTCTTGA TGAGCTGCCA GACACAGTAT ACCGTGTGTG TGACCTGATC ATGACAGCAA TCAAACGTAA TGGAGCAGAT TATCGTGACA TGATTCTGAA GCAAGTAGTC AATCAGGTGT GGGAAGCTGC TGATGTATTG ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CCGTGTCAGA GTGGATAAGT CAGATGGCCA CACTGCCCCA GGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACGCTA CTTTTTGAGG AGTTGAAGCT ACCTTGTGCT TGGGTGGTTG AATCAAGTGG CATCCTTAAT GTCCTAATCA AACTCTTGGA AGTGGTTCAG CCCTGCCTCC AGGCAGCCAA GGAGCAGAAG GAAGTCCAGA CCCCAAAGTG GATCACACCA GTGTTGCTCC TGATTGATTT CTATGAAAAG ACAGCCATCT CCTCAAAAAG GAGAGCCCAG ATGACTAAGT ACCTGCAATC CAACAGCAAC AACTGGCGCT GGTTTGATGA TCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAT AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGCG TGCGATTCAC TGCAGGCCGA AGAAGATACA CGGTCCAATT CACTACAATG GTGCAGGTTA ATGAGGAAAC AGGGAACCGA CGCCCTGTGA TGCTGACTCT CCTCAGGGTA CCTCGGCTGA ATAAAAATTC AAAAAACAGC AATGGACAGG AACTAGAGAA GACGCTGGAA GAAAGCAAAG AAATGGATAT CAAACGTAAA GAAAATAAAG GCAATGATAC CCCTTTGGCC CTAGAGAGTA CAAACACTGA AAAGGAGACA AGCCTGGAGG AAACAAAAAT CGGGGAGATC CTGATCCAGG GCTTGACAGA AGATATGGTG ACTGTTTTAA TCCGGGCCTG CGTGAGCATG CTGGGAGTCC CTGTGGACCC AGATACTTTG CATGCCACCC TTCGTCTCTG TCTGAGGCTC ACCCGGGACC ACAAATATGC CATGATGTTT GCAGAACTGA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG TTTACTCCCC TGGTCACCCT TCTCTTAAGA CACATCATTG AGGACCCCTG TACCCTTCGT CATACCATGG AAAAGGTTGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT GGTGTTGTGT CTGGCAGCCT CGGCTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG CCAGCCGCAT GCCGCAATCC AGACATATTC ACAGAAGTGG CCAACTGCTG TATCCGCATC GCCCTTCCTG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA ATTAAAGGCC CTAATGCTGT ACAGCTGGTG AAGACCACCC CTTTGAAGCC CTCACCTCTG CCTGTCATCC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC TCTGGCTGCA TACCATGCTC CAGAGGAAGA TAAATCTGAT CCTAAACCTG GGGTTATGAC CCAAGAGGTT GGCCAGCTCC TGCAAGACAT GGGTGATGAT GTATACCAGC AGTACCGGTC ACTTACGCGT CAGAGCAGTG ACTTTGATAC GCAGTCAGGT TTTTCCATTA ATAGTCAGGT CTTTGCTGCA GATGGTGCCT CCACTGAGAC TTCCGCATCT GGGACCTCCC AAGGAGAGGC TTCAACTCCA GAGGAGTCTC GAGATGGGAA GAAAGATAAA GAAGGGGACC GGGCCTCTGA GGAAGGCAAA CAGAAAGGCA AGGGCAGCAA ACCTTTAATG CCTACCTCCA CTATCCTTCG TCTTCTGGCA GAGTTGGTGA GGTCCTATGT TGGTATTGCT ACCCTGATTG CCAACTACAG CTACACTGTG GGCCAGTCTG AACTGATCAA AGAGGACTGC AGTGTGCTAG CTTTTGTTCT GGACCACCTG CTCCCACATA CCCAGAATGC AGAAGACAAG GACACCCCTG CCTTGGCCCG CCTGTTCCTC GCAAGCCTGG CTGCTGCAGG GAGTGGCACA GATGCCCAGG TGGCCCTAGT GAATGAAGTA AAAGCAGCCC TTGGACGGGC ACTGGCTATG GCTGAGAGTA CAGAGAAACA TGCCAGGCTT CAGGCAGTGA TGTGTATCAT CAGTACTATC ATGGAGTCCT GCCCCTCCAC CTCCAGCTTC TACAGCAGTG CCACAGCGAA GACCCAGCAC AATGGCATGA ACAACATCAT TCGGCTTTTC CTGAAGAAGG GACTGGTTAA TGACCTGGCC AGAGTACCTC ACAGCTTAGA CCTGTCCAGT CCCAACATGG CCAACACAGT CAATGCTGCT CTGAAGCCTT TGGAAACACT TTCCCGGATT GTGAACCAGC CCAGTAGCCT TTTTGGCAGC AAGAGTGCTT CTAGCAAGAA CAAGTCTGAG CAGGATGCCC AAGGAGCCTC TCAAGATTCC AGTAGCAACC AGCAGGACCC AGGCGAGCCT GGGGAAGCAG AAGTGCAGGA GGAGGATCAT GATGTCACTC AGACAGAGGT GGCAGATGGG GATATCATGG ATGGGGAGGC TGAAACCGAC TCAGTGGTGA TTGCTGGGCA GCCTGAGGTG CTCAGTTCAC AAGAGATGCA GGTTGAGAAT GAGCTGGAGG ACCTGATAGA TGAGTTGCTT GAGAGGGATG GCGGATCTGG GAACAGTACA ATTATAGTGA GCAGAAGTGG AGAGGATGAA TCACAAGAGG ACGTGCTGAT GGATGAAGCT CCTTCCAACC TCAGCCAAGC TTCCACCTTG CAGGCCAACC GAGAAGATTC CATGAATATC CTGGACCCTG AGGATGAGGA GGAGCACACT CAGGAAGAGG ACAGCAGTGG CAGTAACGAG GATGAGGATG ATAGTCAGGA TGAAGAGGAG GAGGAGGAGG AAGATGAGGA AGATGATCAG GAGGATGATG AAGGTGAAGA GGGAGATGAA GACGATGACG ACGATGGCTC TGAGATGGAA TTGGATGAGG ATTATCCTGA TATGAACGCT TCTCCCTTGG TCCGATTTGA GCGCTTTGAC CGGGAGGATG ATCTCATCAT TGAGTTTGAC AACATGTTCT CCAGTGCTAC AGACATCCCC CCATCCCCAG GAAATATCCC TACCACCCAT CCACTGATGG TGCGCCATGC AGACCACAGT TCTCTGACAC TGGGCAGTGG CTCTTCAACA ACTCGTCTCA CCCAGGGCAT CGGGCGCAGT CAGAGGACCC TAAGGCAGCT GACGGCCAAT ACTGGCCACA CCATTCATGT TCACTACCCT GGGAATCGCC AGCCCAACCC TCCTCTTATA CTGCAGAGGT TGCTTGGTCC CTCAGCTGCT GCTGACATCC TTCAGCTGAG CAGCAGCCTT CCCCTACAAA GCCGGGGTCG GGCCCGCCTC CTGGTAGGCA ACGATGACGT CCACATCATC GCCCGTTCTG ATGATGAGCT GCTGGATGAC TTTTTCCATG ATCAGAGCAC AGCTACCAGC CAAGCAGGAA CCCTGTCCAG CATCCCCACA GCCCTGACCC GCTGGACAGA AGAATGCAAA GTTCTCGATG CTGAGAGCAT GCATGACTGT GTTTCAGTGG TTAAAGTGTC CATTGTCAAT CACCTGGAAT TCCTGAGGGA TGAGGAGCTG GAAGAAAGGC GAGAGAAGCG CAGGAAACAA CTGGCTGAGG AAGAAACAAA GATAACTGAT AAAGGCAAAG AAGATAAGGA GAACAGGGAT CAGAGTGCAC AGTGTACTGC ATCTAAGTCA AATGACTCCA CTGAACAGAA TCTCTCAGAT GGGACGCCTA TGCCTGACAG CTACCCAACA ACCCCATCTT CAACTGATGC AGCTACATCT GAGTCCAAGG AGACCCTTGG CACTCTGCAA TCCTCACAAC AGCAACCAAC ACTCCCAACC CCACCAGCTT TGGGAGAGGT TCCTCAGGAG CTGCAGTCTC CAGCTGGAGA AGGGGGCAGC TCTACACAGC TATTGATGCC TGTAGAGCCA GAGGAATTGG GTCCCACAAG GCCAAGTGGG GAAGCAGAAA CAACTCAGAT GGAGTTATCC CCAGCTCCCA CTATAACCTC ACTTTCCCCA GAGAGAGCTG AGGATTCTGA TGCACTGACG GCTGTCAGCA GTCAGCTAGA AGGCTCTCCT ATGGATACAA GCAGCCTGGC TTCCTGTACC TTAGAGGAGG CTGTGGGTGA CACTTCAGCA GCTGGCAGTT CTGAGCAGCC CAGAGCAGGC AGCTCCACTC CTGGGGATGC CCCACCAGCT GTGGCGGAAG TGCAAGGCAG GAGTGATGGG TCAGGGGAAT CTGCCCAGCC ACCTGAGGAC AGCTCCCCAC CTGCATCCTC TGAGAGCTCT TCCACCAGAG ATTCTGCCGT GGCCATTTCT GGAGCAGATT CCCGAGGAAT CCTAGAAGAG CCGTTGCCTT CAACAAGCAG TGAAGAAGAA GATCCCCTTG CGGGTATCAG TCTCCCTGAA GGTGTGGACC CCTCTTTTCT GGCTGCCCTG CCTGATGACA TCCGTCGGGA AGTTCTACAG AACCAGCTAG GCATTCGTCC ACCAACCCGG ACTGCCCCCT CCACAAATAG CTCAGCGCCT GCAGTGGTGG GGAATCCTGG TGTGACTGAA GTACTGGCAC AGCAGAGAGC TGAGCAGCAG CGACGAGAAC TAGCACAGAA TGCCAGCTCA GACACCCCTA TGGACCCTGT GACCTTCATC CAGACTCTGC CCTCAGACCT GCGCCGTAGT GTCCTAGAGG ATATGGAGGA CAGTGTGTTA GCTGTGATGC CACCTGACAT TGCAGCTGAG GCTCAAGCCC TGAGACGAGA GCAAGAAGCC CGGCAGCGAC AGCTCATGCA TGAGCGTCTG TTTGGGCACA GTAGCACCTC CGCACTCTCT GCTATTCTCC GAAGCCCGGC TTTCACCAGT CGCTTAAGTG GCAACCGTGG GGTCCAGTAT ACTCGCCTTG CTGTGCAGAG AGGTGGCACC TTCCAGATGG GGGGTAGCAG CAGCCATAAC AGGCCTTCTG GCAGTAATGT AGATACTCTC CTCCGCCTCC GAGGACGGCT CCTTCTGGAC CACGAAGCCC TTTCTTGTCT CTTGGTCCTA CTTTTTGTGG ATGAGCCAAA GCTCAATACT AGCCGTCTAC ACCGAGTACT GAGAAATCTC TGCTACCATG CCCAGACCCG CCACTGGGTC ATCCGCAGTC TGCTCTCCAT CTTGCAGCGC AGCAGTGAGA GTGAGCTATG CATTGAAACA CCCAAACTCA CTACAAGTGA GGAAAAGGGC AAAAAGTCGA GCAAGAGCTG TGGGTCAAGT AGCCATGAGA ACCGTCCCCT GGACCTGCTA CACAAGATGG AGTCAAAGAG CTCCAACCAG CTTTCCTGGC TCTCAGTATC CATGGATGCA GCCCTAGGCT GCAGGACTAA TATATTTCAG ATCCAGCGTT CAGGGGGGCG TAAACATACC GAGAAGCATG CAAGCGGTGG CTCCACCGTC CACATCCATC CCCAAGCTGC TCCTGTTGTC TGCAGACACG TTTTGGATAC ACTCATTCAA TTGGCCAAGG TATTTCCCAG CCACTTCACA CAGCAGCGGA CCAAAGAAAC AAACTGTGAG AGTGATCGGG AAAGGGGCAA TAAGGCCTGT AGCCCATGCT CCTCACAGTC CTCCAGCAGT GGCATTTGCA CAGACTTCTG GGACTTATTG GTAAAACTGG ACAACATGAA TGTCAGCCGG AAAGGCAAGA ACTCCGTGAA GTCAGTGCCA GTGAGCGCTG GCGGTGAGGG GGAAACCTCT CCATACAGCC TCGAGGCCTC TCCACTGGGG CAGCTCATGA ACATGTTGTC ACACCCAGTC ATCCGCCGGA GCTCTCTCTT AACTGAGAAA CTCCTCAGAC TCCTTTCTCT CATCTCAATT GCTCTCCCAG AAAACAAGGT GTCAGAAGCA CAGGCTAATT CTGGCAGCGG TGCTTCCTCC ACCACCACTG CCACCTCAAC CACATCTACC ACCACCACCA CTGCCGCCTC CACCACGCCC ACACCCCCTA CTGCACCCAC CCCTGTCACT TCTGCTCCAG CCCTGGTTGC TGCCACGGCT ATTTCCACCA TTGTCGTAGC TGCTTCGACC ACAGTGACTA CCCCCACGAC TGCTACCACT ACTGTTTCAA TTTCTCCCAC TACTAAGGGC AGCAAATCTC CAGCGAAGGT GAGTGATGGG GGCAGCAGCA GTACAGACTT TAAGATGGTG TCCTCTGGCC TCACTGAAAA CCAGCTACAG CTCTCTGTAG AGGTGTTGAC ATCCCACTCT TGTTCTGAGG AAGGCTTAGA GGATGCAGCC AACGTACTAC TGCAGCTCTC CCGGGGGGAC TCTGGGACCC GGGACACTGT TCTCAAGCTG CTACTGAATG GAGCCCGCCA TCTGGGTTAT ACCCTTTGTA AACAAATAGG TACCCTGCTG GCCGAGCTGC GGGAATACAA CCTCGAGCAG CAGCGGCGAG CCCAATGTGA AACCCTCTCT CCTGATGGCC TGCCTGAGGA GCAGCCACAG ACCACCAAGC TGAAGGGCAA AATGCAGAGC AGGTTTGACA TGGCTGAGAA TGTGGTAATT GTGGCATCTC AGAAGCGACC TTTGGGTGGC CGGGAGCTCC AGCTGCCTTC TATGTCCATG TTGACATCCA AGACATCTAC CCAGAAGTTC TTCTTGAGGG TACTACAGGT CATCATCCAG CTCCGGGACG ACACGCGCCG GGCTAACAAG AAAGCCAAGC AGACAGGCAG GCTAGGTTCC TCCGGTTTAG GCTCAGCTAG CAGCATCCAG GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT GATGCCATTA TACAAATGGT ACGTGAGGGT CAAAGGGCGC GGAGACAGCA ACAAGCAGCA ACGTCGGAGT CTAGCCAGTC AGAGGCGTCT GTCCGGAGGG AGGAATCACC CATGGATGTG GACCAGCCAT CTCCCAGTGC TCAAGATACT CAATCCATTG CCTCCGATGG AACCCCACAG GGGGAGAAGG AAAAGGAAGA AAGACCACCT GAGTTACCCC TGCTCAGCGA GCAGCTGAGT TTGGACGAGC TGTGGGACAT GCTTGGGGAG TGTCTAAAGG AACTAGAGGA ATCCCATGAC CAGCATGCGG TGCTAGTGCT ACAGCCTGCT GTCGAGGCCT TCTTTCTGGT CCATGCCACA GAGCGGGAGA GCAAGCCTCC TGTCCGAGAC ACCCGTGAGA GCCAGCTGGC ACACATCAAG GACGAGCCTC CTCCACTCTC CCCTGCCCCC TTAACCCCAG CCACGCCTTC CTCCCTTGAC CCATTCTTCT CCCGGGAGCC CTCATCTATG CACATCTCCT CAAGCCTGCC CCCTGACACA CAGAAGTTCC TTCGCTTTGC AGAGACTCAC CGCACTGTGT TAAACCAGAT CCTACGGCAG TCCACGACCC ACCTTGCTGA TGGGCCTTTT GCTGTCCTGG TAGACTACAT TCGTGTCCTC GACTTTGATG TCAAGCGCAA ATATTTCCGC CAAGAGCTGG AGCGTTTAGA TGAGGGGCTC CGGAAAGAAG ACATGGCTGT GCATGTCCGT CGTGACCATG TGTTTGAAGA CTCCTATCGT GAGCTGCATC GCAAATCCCC CGAAGAAATG AAGAATCGAT TGTATATAGT ATTTGAAGGA GAAGAAGGGC AGGATGCTGG CGGGCTCCTG CGGGAGTGGT ATATGATCAT CTCTCGAGAG ATGTTTAACC CTATGTATGC CTTGTTCCGT ACCTCACCTG GTGATCGAGT CACCTACACC ATCAATCCAT CTTCCCACTG CAACCCCAAC CACCTCAGCT ACTTCAAGTT TGTCGGACGC ATTGTGGCCA AAGCTGTATA TGACAACCGT CTTCTGGAGT GCTACTTTAC TCGATCCTTT TACAAACACA TCTTGGGCAA GTCAGTCAGA TATACAGATA TGGAGAGTGA AGATTACCAC TTCTACCAAG GTCTGGTTTA TCTGCTGGAA AATGATGTCT CCACACTAGG CTATGACCTC ACCTTCAGCA CTGAGGTCCA AGAGTTTGGA GTTTGTGAAG TTCGTGACCT CAAACCCAAT GGGGCCAACA TCTTGGTAAC AGAGGAGAAT AAGAAGGAGT ATGTACACCT GGTATGCCAG ATGAGAATGA CAGGAGCCAT CCGCAAGCAG TTGGCGGCTT TCTTAGAAGG CTTCTATGAG ATCATTCCAA AGCGCCTCAT TTCCATCTTC ACTGAGCAGG AGTTAGAGCT GCTTATATCA GGACTGCCCA CCATTGACAT CGATGATCTG AAATCCAACA CTGAATACCA CAAGTACCAG TCCAACTCTA TTCAGATCCA GTGGTTCTGG AGAGCATTGC GTTCTTTCGA TCAAGCTGAC CGTGCCAAGT TCCTCCAGTT TGTCACGGGT ACTTCCAAGG TACCCCTGCA AGGCTTTGCT GCCCTCGAAG GCATGAATGG CATTCAGAAG TTTCAGATCC ATCGAGATGA CAGGTCCACA GATCGCCTGC CTTCAGCTCA CACATGTTTT AATCAGCTGG ATCTGCCTGC CTATGAGAGC TTTGAGAAGC TCCGCCACAT GCTACTGTTG GCTATCCAGG AGTGCTCTGA AGGCTTTGGG CTGGCCCTCG AGTACCCATA CGACGTACCT GATTATGCAA CTTATCCTTA TGATGTACCT GATTATGCTA GCTATCCTTA TGATGTCCCA GACTACGCAT AGTGAGGGCC CGATATCTCG ACAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAGCT GACGTCCTTT CCATGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG CCTGGAAACG GGGGAGGCTA ACTGAAACAC GGAAGGAGAC AATACCGGAA GGAACCCGCG CTATGACGGC AATAAAAAGA CAGAATAAAA CGCACGGGTG TTGGGTCGTT TGTTCATAAA CGCGGGGTTC GGTCCCAGGG CTGGCACTCT GTCGATACCC CACCGAGACC CCATTGGGGC CAATACGCCC GCGTTTCTTC CTTTTCCCCA CCCCACCCCC CAAGTTCGGG TGAAGGCCCA GGGCTCGCAG CCAACGTCGG GGCGGCAGGC CCTGCCATAG CAGATCTGCG CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGCATCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGGGG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC AATGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA
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