pLV2-TRE3GS-SV40 LT(human)-TetOne-Puro
$99.00
In stock
SKU
PL37464
100μg liquid plasmid(Instantly):$120
| name | pLV2-TRE3GS-SV40 LT(human)-TetOne-Puro | another name | apLV2-TRE3GS-SV40 LT(human)-TetOne-Puro |
| Insert Size (bp) | 2127bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression,Tetracycline induction | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | TRE3GS | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGGAAGGGCT AATTCACTCC CAAAGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA CACAAGGCTA CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA GAAGAGGCCA ATAAAGGAGA GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG AGAGAGAAGT GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC GTGGCCCGAG AGCTGCATCC GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG GGACTTTCCG CTGGGGACTT TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA GCCCTCAGAT CCTGCATATA AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGCCTTT AAAAGAAAAG GGGGGATTGG GGGGTACAGT GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC AAACTAAAGA ACTACAAAAA CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG ACAGCAGAGA TCCAGTTTAT CGACTTAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAGGCAATAG CATCACAAAT TTCACAAATA AGGCATTTTT TTCACTGCAT TCTAGTTTTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGGAT CTCAAATCCC TCGGAAGCTG CGCCTGTCTT AGGTTGGAGT GATACATTTT TATCACTTTT ACCCGTCTTT GGATTAGGCA GTAGCTCTGA CGGCCCTCCT GTCTTAGGTT AGTGAAAAAT GTCACTCTCT TACCCGTCAT TGGCTGTCCA GCTTAGCTCG CAGGGGAGGT GGTCTGGATC CTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA TGGCTGATTA TGATCATGAA CAGACTGTGA GGACTGAGGG GCCTGAAATG AGCCTTGGGA CTGTGAATCA ATGCCTGTTT CATGCCCTGA GTCTTCCATG TTCTTCTCCC CACCATCTTC ATTTTTATCA GCATTTTCCT GGCTGTCTTC ATCATCATCA TCACTGTTTC TTAGCCAATC TAAAACTCCA ATTCCCATAG CCACATTAAA CTTCATTTTT TGATACACTG ACAAACTAAA CTCTTTGTCC AATCTCTCTT TCCACTCCAC AATTCTGCTC TGAATACTTT GAGCAAACTC AGCCACAGGT CTGTACCAAA TTAACATAAG AAGCAAAGCA ATGCCACTTT GAATTATTCT CTTTTCTAAC AAAAACTCAC TGCGTTCCAG GCAATGCTTT AAATAATCTT TGGGCCTAAA ATCTATTTGT TTTACAAATC TGGCCTGCAG TGTTTTAGGC ACACTGTACT CATTCATGGT GACTATTCCA GGGGGAAATA TTTGAGTTCT TTTATTTAGG TGTTTCTTTT CTAAGTTTAC CTTAACACTG CCATCCAAAT AATCCCTTAA ATTGTCCAGG TTATTAATTC CCTGACCTGA AGGCAAATCT CTGGACTCCC CTCCAGTGCC CTTTACATCC TCAAAAACTA CTAAAAACTG GTCAATAGCT ACTCCTAGCT CAAAGTTCAG CCTGTCCAAG GGCAAATTAA CATTTAAAGC TTTCCCCCCA CATAATTCAA GCAAAGCAGC TGCTAATGTA GTTTTACCAC TATCAATTGG TCCTTTAAAC AGCCAGTATC TTTTTTTAGG AATGTTGTAC ACCATGCATT TTAAAAAGTC ATACACCACT GAATCCATTT TGGGCAACAA ACAGTGTAGC CAAGCAACTC CAGCCATCCA TTCTTCTATG TCAGCAGAGC CTGTAGAACC AAACATTATA TCCATCCTAT CCAAAAGATC ATTAAATCTG TTTGTTAACA TTTGTTCTCT AGTTAATTGT AGGCTATCAA CCCGCTTTTT AGCTAAAACA GTATCAACAG CCTGTTGGCA TATGGTTTTT TGGTTTTTGC TGTCAGCAAA TATAGCAGCA TTTGCATAAT GCTTTTCATG GTACTTATAG TGGCTGGGCT GTTCTTTTTT AATACATTTT AAACACATTT CAAAACTGTA CTGAAATTCC AAGTACATCC CAAGCAATAA CAACACATCA TCACATTTTG TTTCCATTGC ATACTCTGTT ACAAGCTTCC AGGACACTTG TTTAGTTTCC TCTGCTTCTT CTGGATTAAA ATCATGCTCC TTTAACCCAC CTGGCAAACT TTCCTCAATA ACAGAAAATG GATCTCTAGT CAAGGCACTA TACATCAAAT ATTCCTTATT AACCCCTTTA CAAATTAAAA AGCTAAAGGT ACACAATTTT TGAGCATAGT TATTAATAGC AGACACTCTA TGCCTGTGTG GAGTAAGAAA AAACAGTATG TTATGATTAT AACTGTTATG CCTACTTATA AAGGTTACAG AATATTTTTC CATAATTTTC TTGTATAGCA GTGCAGCTTT TTCCTTTGTG GTGTAAATAG CAAAGCAAGC AAGAGTTCTA TTACTAAACA CAGCATGACT CAAAAAACTT AGCAATTCTG AAGGAAAGTC CTTGGGGTCT TCTACCTTTC TCTTCTTTTT TGGAGGAGTA GAATGTTGAG AGTCAGCAGT AGCCTCATCA TCACTAGATG GCATTTCTTC TGAGCAAAAC AGGTTTTCCT CATTAAAGGC ATTCCACCAC TGCTCCCATT CATCAGTTCC ATAGGTTGGA ATCTCAGTTG CATCCCAGAA GCCTCCAAAG TCAGGTTGAT GAGCATATTT TACTCCATCT TCCATTTTCT TGTACAGAGT ATTCATTTTC TTCATTTTTT CTTCATCTCC TCCTTTATCA GGATGAAACT CCTTGCATTT TTTTAAATAT GCCTTTCTCA TCAGAGGAAT ATTCCCCCAG GCACTCCTTT CAAGACCTAG AAGGTCCATT AGCTGCAAAG ATTCCTCTCT GTTTAAAACT TTATCCATGG TGGCGAATTC TTTACGAGGG TAGGAAGTGG TACGGAAAGT TGGTATAAGA CAAAAGTGTT GTGGAATTGA AGTTTACTCA AAAAATCAGC ACTCTTTTAT AGGCGCCCTG GTTTACATAA GCAAAGCTTA TACGTTCTCT ATCACTGATA GGGAGTAAAC TGGATATACG TTCTCTATCA CTGATAGGGA GTAAACTGTA GATACGTTCT CTATCACTGA TAGGGAGTAA ACTGGTCATA CGTTCTCTAT CACTGATAGG GAGTAAACTC CTTATACGTT CTCTATCACT GATAGGGAGT AAAGTCTGCA TACGTTCTCT ATCACTGATA GGGAGTAAAC TCTTCATACG TTCTCTATCA CTGATAGGGA GTAAACTCGA GGTGATAATT CCACGGGGTT GGGGTTGCGC CTTTTCCAAG GCAGCCCTGG GTTTGCGCAG GGACGCGGCT GCTCTGGGCG TGGTTCCGGG AAACGCAGCG GCGCCGACCC TGGGTCTCGC ACATTCTTCA CGTCCGTTCG CAGCGTCACC CGGATCTTCG CCGCTACCCT TGTGGGCCCC CCGGCGACGC TTCCTGCTCC GCCCCTAAGT CGGGAAGGTT CCTTGCGGTT CGCGGCGTGC CGGACGTGAC AAACGGAAGC CGCACGTCTC ACTAGTACCC TCGCAGACGG ACAGCGCCAG GGAGCAATGG CAGCGCGCCG ACCGCGATGG GCTGTGGCCA ATAGCGGCTG CTCAGCAGGG CGCGCCGAGA GCAGCGGCCG GGAAGGGGCG GTGCGGGAGG CGGGGTGTGG GGCGGTAGTG TGGGCCCTGT TCCTGCCCGC GCGGTGTTCC GCATTCTGCA AGCCTCCGGA GCGCACGTCG GCAGTCGGCT CCCTCGTTGA CCGAATCACC GACCTCTCTC CCCAGGGGGA TCATCGAATT ACCATGTCTA GACTGGACAA GAGCAAAGTC ATAAACTCTG CTCTGGAATT ACTCAATGGA GTCGGTATCG AAGGCCTGAC GACAAGGAAA CTCGCTCAAA AGCTGGGAGT TGAGCAGCCT ACCCTGTACT GGCACGTGAA GAACAAGCGG GCCCTGCTCG ATGCCCTGCC AATCGAGATG CTGGACAGGC ATCATACCCA CTCCTGCCCC CTGGAAGGCG AGTCATGGCA AGACTTTCTG CGGAACAACG CCAAGTCATA CCGCTGTGCT CTCCTCTCAC ATCGCGACGG GGCTAAAGTG CATCTCGGCA CCCGCCCAAC AGAGAAACAG TACGAAACCC TGGAAAATCA GCTCGCGTTC CTGTGTCAGC AAGGCTTCTC CCTGGAGAAC GCACTGTACG CTCTGTCCGC CGTGGGCCAC TTTACACTGG GCTGCGTATT GGAGGAACAG GAGCATCAAG TAGCAAAAGA GGAAAGAGAG ACACCTACCA CCGATTCTAT GCCCCCACTT CTGAAACAAG CAATTGAGCT GTTCGACCGG CAGGGAGCCG AACCTGCCTT CCTTTTCGGC CTGGAACTAA TCATATGTGG CCTGGAGAAA CAGCTAAAGT GCGAAAGCGG CGGGCCGACC GACGCCCTTG ACGATTTTGA CTTAGACATG CTCCCAGCCG ATGCCCTTGA CGACTTTGAC CTTGATATGC TGCCTGCTGA CGCTCTTGAC GATTTTGACC TTGACATGCT CCCCGGGTAA ACGCGCGAAT CGCCCCTCTC CCTCCCCCCC CCCTAACGTT ACTGGCCGAA GCCGCTTGGA ATAAGGCCGG TGTGCGTTTG TCTATATGTT ATTTTCCACC ATATTGCCGT CTTTTGGCAA TGTGAGGGCC CGGAAACCTG GCCCTGTCTT CTTGACGAGC ATTCCTAGGG GTCTTTCCCC TCTCGCCAAA GGAATGCAAG GTCTGTTGAA TGTCGTGAAG GAAGCAGTTC CTCTGGAAGC TTCTTGAAGA CAAACAACGT CTGTAGCGAC CCTTTGCAGG CAGCGGAACC CCCCACCTGG CGACAGGTGC CTCTGCGGCC AAAAGCCACG TGTATAAGAT ACACCTGCAA AGGCGGCACA ACCCCAGTGC CACGTTGTGA GTTGGATAGT TGTGGAAAGA GTCAAATGGC TCTCCTCAAG CGTATTCAAC AAGGGGCTGA AGGATGCCCA GAAGGTACCC CATTGTATGG GATCTGATCT GGGGCCTCGG TGCACATGCT TTACATGTGT TTAGTCGAGG TTAAAAAAAC GTCTAGGCCC CCCGAACCAC GGGGACGTGG TTTTCCTTTG AAAAACACGA TGATAAGCTT GCCACAACCC ACAAGGAGAC GACCTTCCAT GACCGAGTAC AAGCCCACGG TGCGCCTCGC CACCCGCGAC GACGTCCCCC GGGCCGTACG CACCCTCGCC GCCGCGTTCG CCGACTACCC CGCCACGCGC CACACCGTCG ACCCGGACCG CCACATCGAG CGGGTCACCG AGCTGCAAGA ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG TGGGTCGCGG ACGACGGCGC CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC GCGCAGCAAC AGATGGAAGG CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC CTGGCCACCG TCGGCGTCTC GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG CCCCGCAACC TCCCCTTCTA CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG CCCGAAGGAC CGCGCACCTG GTGCATGACC CGCAAGCCCG GTGCCTAGTT TAAACCCGCT GATCAGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAC AATAGCAGGC ATGCTGGGGA TGCGGTGGGC TCTATGGACG CGTCTGGAAC AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC ATGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCC TGGAATTAAT TCTGCAGTCG AGACCTAGAA AAACATGGAG CAATCACAAG TAGCAATACA GCAGCTACCA ATGCTGATTG TGCCTGGCTA GAAGCACAAG AGGAGGAGGA GGTGGGTTTT CCAGTCACAC CTCAGGTACC TTTAAGACCA ATGACTTACA AGGCAGCTGT AGATCTTAGC CACTTTTTAA AAGAAAAGAG GGGACTGGAA GGGCTAATTC ACTCCCAACG AAGACAAGAT ATCCTTGATC TGTGGATCTA CCACACACAA GGCTACTTCC CTGATTAGCA GAACTACACA CCAGGGCCAG GGGTCAGATA TCCACTGACC TTTGGATGGT GCTACAAGCT AGTACCAGTT GAGCCAGATA AGGTAGAAGA GGCCAATAAA GGAGAGAACA CCAGCTTGTT ACACCCTGTG AGCCTGCATG GGATGGATGA CCCGGAGAGA GAAGTGTTAG AGTGGAGGTT TGACAGCCGC CTAGCATTTC ATCACGTGGC CCGAGAGCTG CATCCGGAGT ACTTCAAGAA CTGCTGATAT CGAGCTTGCT ACAAGGGACT TTCCGCTGGG GACTTTCCAG GGAGGCGTGG CCTGGGCGGG ACTGGGGAGT GGCGAGCCCT CAGATCCTGC ATATAAGCAG CTGCTTTTTG CCTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG TAGTAGTTCA TGTCATCTTA TTATTCAGTA TTTATAACTT GCAAAGAAAT GAATATCAGA GAGTGAGAGG CCTTGACATT GCTAGCGTTT TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCGACGGATC GGGAGATCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG ATCAACTGGA TAACTCAAGC TAACCAAAAT CATCCCAAAC TTCCCACCCC ATACCCTATT ACCACTGCCA ATTACCTAGT GGTTTCATTT ACTCTAAACC TGTGATTCCT CTGAATTATT TTCATTTTAA AGAAATTGTA TTTGTTAAAT ATGTACTACA AACTTAGTAG TTTTTAAAGA AATTGTATTT GTTAAATATG TACTACAAAC TTAGTAGT
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