NM_198253.3;CMM9; DKCA2; DKCB4; EST2; hEST2; hTRT;PFBMFT1; TCS1; TP2; TRT
100μg liquid plasmid(Instantly):$134
| name | pLV2-TRE3GS-TERT(human)-TetOne-Puro | another name | apLV2-TRE3GS-TERT(human)-TetOne-Puro |
| Insert Size (bp) | 3399bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression,Tetracycline induction | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | TRE3GS | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGGAAGGGCT AATTCACTCC CAAAGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA CACAAGGCTA CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA GAAGAGGCCA ATAAAGGAGA GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG AGAGAGAAGT GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC GTGGCCCGAG AGCTGCATCC GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG GGACTTTCCG CTGGGGACTT TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA GCCCTCAGAT CCTGCATATA AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGCCTTT AAAAGAAAAG GGGGGATTGG GGGGTACAGT GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC AAACTAAAGA ACTACAAAAA CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG ACAGCAGAGA TCCAGTTTAT CGACTTAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAGGCAATAG CATCACAAAT TTCACAAATA AGGCATTTTT TTCACTGCAT TCTAGTTTTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGGAT CTCAAATCCC TCGGAAGCTG CGCCTGTCTT AGGTTGGAGT GATACATTTT TATCACTTTT ACCCGTCTTT GGATTAGGCA GTAGCTCTGA CGGCCCTCCT GTCTTAGGTT AGTGAAAAAT GTCACTCTCT TACCCGTCAT TGGCTGTCCA GCTTAGCTCG CAGGGGAGGT GGTCTGCGGC CGCTCAGTCC AGGATGGTCT TGAAGTCTGA GGGCAGTGCC GGGTTGGCTG CGGCCTCCAG GGCAGTCAGC GTCGTCCCCG GGAGCTTCCG ACTCAGCTGC GTCTGGGCTG TCCTGAGTGA CCCCAGGAGT GGCACGTAGG TGACACGGTG TCGAGTCAGC TTGAGCAGGA ATGCTTGGTG GCACAGCCAC TGCACGGCCT CGGAGGGCAG AGGGCCGGCG GCGCCCTTGG CCCCCAGCGA CATCCCTGCG TTCTTGGCTT TCAGGATGGA GTAGCAGAGG GAGGCCGTGT CAGAGATGAC GCGCAGGAAA AATGTGGGGT TCTTCCAAAC TTGCTGATGA AATGGGAGCT GCAGCACACA TGCGTGAAAC CTGTACGCCT GCAGCAGGAG GATCTTGTAG ATGTTGGTGC ACACCGTCTG GAGGCTGTTC ACCTGCAAAT CCAGAAACAG GCTGTGACAC TTCAGCCGCA AGACCCCAAA GAGTTTGCGA CGCATGTTCC TCCCAGCCTT GAAGCCGCGG TTGAAGGTGA GACTGGCTCT GATGGAGGTC CGGGCATAGC TGGAGTAGTC GCTCTGCACC TCCAGGGTCC GGGTATCCAG CAGCAGGCCG CACCAGGGGA ATAGGCCGTG GGCCGGCATC TGAACAAAAG CCGTGCCACC CAGGGCCTCG TCTTCTACAG GGAAGTTCAC CACTGTCTTC CGCAAGTTCA CCACGCAGCC ATACTCAGGG ACACCTCGGA CCAGGGTCCT GAGGAAGGTT TTCGCGTGGG TGAGGTGAGG TGTCACCAAC AAGAAATCAT CCACCAAACG CAGGAGCAGC CCGTCCCGCC GAATCCCCGC AAACAGCTTG TTCTCCATGT CGCCGTAGCA CAGGCTGCAG AGCAGCGTGG AGAGGATGGA GCCCTGCGGG ATCCCCTGGC ACTGGACGTA GGACTTGCCC CTGATGCGCA CGGCGTGGTG GCACATGAAG CGTAGGAAGA CGTCGAAGAG GCCACTGCTG GCCTCATTCA GGGAGGAGCT CTGCTCGATG ACGACGGCAT CCCTCAGCGG GCTGGTCTCC TGCAGGTGAG CCACGAACTG TCGCATGTAC GGCTGGAGGT CTGTCAAGGT AGAGACGTGG CTCTTGAAGG CCTTGCGGAC GTGCCCATGG GCGGCCTTCT GGACCACGGC ATACCGACGC ACGCAGTACG TGTTCTGGGG TTTGATGATG CTGGCGATGA CCTCCGTGAG CCTGTCCTGG GGGATGGTGT CGTACGCGCC CGTCACATCC ACCTTGACAA AGTACAGCTC AGGCGGCGGG TCCTGGGCCC GCACACGCAG CACGAAGGTG CGCCAGGCCC TGTGGATATC GTCCAGGCCC AGCACAGAGG CGCCCAGGAG GCCGGGGCGC CGCGCCCGCT CGTAGTTGAG CACGCTGAAC AGTGCCTTCA CCCTCGAGGT GAGACGCTCG GCCCTCTTTT CTCTGCGGAA CGTTCTGGCT CCCACGACGT AGTCCATGTT CACAATCGGC CGCAGCCCGT CAGGCTTGGG GATGAAGCGG AGTCTGGACG TCAGCAGGGC GGGCCTGGCT TCCCGATGCT GCCTGACCTC TGCTTCCGAC AGCTCCCGCA GCTGCACCCT CTTCAAGTGC TGTCTGATTC CAATGCTTTG CAACTTGCTC CAGACACTCT TCCGGTAGAA AAAGAGCCTG TTCTTTTGAA ACGTGGTCTC CGTGACATAA AAGAAAGACC TGAGCAGCTC GACGACGTAC ACACTCATCA GCCAGTGCAG GAACTTGGCC AGGATCTCCT CACGCAGACG GTGCTCTGCG GCCGGAACAC AGCCAACCCC TGGGCTCCTG CGCAGCCAAG CGCAGTCCCG CACGCTCATC TTCCACGTCA GCTCCTGCAG CGAGAGCTTG GCATGCTTCC CCAGGGAGAT GAACTTCTTG GTGTTCCTGA GGAAGCGGCG TTCGTTGTGC CTGGAGCCCC AGAGGCCTGG GGGCACCAGC CGGCGCAGGC AGGCCCGCAC GAAGCCGTAC ACCTGCCAGG GGCTGCTGTG CTGGCGGAGC AGCTGCACCA GGCGACGGGG GTCTGTGTCC TCCTCCTCGG GGGCCGCCAC AGAGCCCTGG GGCTTCTCCC GGGCACAGAC ACCGGCTGCT GGGGTGACCG CAGCTCGCAG CGGGCAGTGC GTCTTGAGGA GCACCCCGTA GGGGCACTGC GCGTGGTTCC CAAGCAGCTC CAGAAACAGG GGCCGCATTT GCCAGTAGCG CTGGGGCAGG CGGGGCAACC TGCGGGGAGT CCCTGGCATC CAGGGCCTGG AACCCAGAAA GATGGTCTCC ACGAGCCTCC GAGCGCCAGT CAGGCTGGGC CTCAGAGAGC TGAGTAGGAA GGAGGGCCGC AGCTGCTCCT TGTCGCCTGA GGAGTAGAGG AAGTGCTTGG TCTCGGCGTA CACCGGGGGA CAAGGCGTGT CCCAGGGACG TGGTGGCCGC GATGTGGATG GGGGGCCCGC GTGGTGCTGG CGGCCCACGG ATGGGTGGGA GTGGCGCGTG CCAGAGAGCG CACCCTCCAA AGAGGTGGCT TCTTCGGCGG GTCTGGCAGG TGACACCACA CAGAAACCAC GGTCACTCGG TCCACGCGTC CTGCCCGGGT GGGCCCAGGA CCCCTGCCCA ACGGGCGTCC GCTCCGGCTC AGGGGCAGCG CCACGCCTGG GCCTCTTGGG CAACGGCAGA CTTCGGCTGG CACTGCCCCC GCGCCTCCTC GCACCCGGGG CTGGCAGGCC CAGGGGGACC CCGGCCTCCC TGACGCTATG GTTCCAGGCC CGTTCGCATC CCAGACGCCT TCGGGGTCCA CTAGCGTGTG GCGGGGGCCG GGCCTGAGTG GCAGCGCCGA GCTGGTACAG CGGCGGCCCG CACACCTGGT AGGCGCAGCT GGGAGCCACC AGCACAAAGA GCGCGCAGCG TGCCAGCAGG TGAACCAGCA CGTCGTCGCC CACGCGGCGC AGCAGCAGCC CCCACGCCCC GCTCCCCCGC AGTGCGTCGG TCACCGTGTT GGGCAGGTAG CTGCGCACGC TGGTGGTGAA GGCCTCGGGG GGGCCCCCGC GGGCCCCGTC CAGCAGCGCG AAGCCGAAGG CCAGCACGTT CTTCGCGCCG CGCTCGCACA GCCTCTGCAG CACTCGGGCC ACCAGCTCCT TCAGGCAGGA CACCTGGCGG AAGGAGGGGG CGGCGGGGGG CGGCCGTGCG TCCCAGGGCA CGCACACCAG GCACTGGGCC ACCAGCGCGC GGAAAGCCGC CGGGTCCCCG CGCTGCACCA GCCGCCAGCC CTGGGGCCCC AGGCGCCGCA CGAACGTGGC CAGCGGCAGC ACCTCGCGGT AGTGGCTGCG CAGCAGGGAG CGCACGGCTC GGCAGCGGGG AGCGCGCGGC ATGGTGGCGA ATTCTTTACG AGGGTAGGAA GTGGTACGGA AAGTTGGTAT AAGACAAAAG TGTTGTGGAA TTGAAGTTTA CTCAAAAAAT CAGCACTCTT TTATAGGCGC CCTGGTTTAC ATAAGCAAAG CTTATACGTT CTCTATCACT GATAGGGAGT AAACTGGATA TACGTTCTCT ATCACTGATA GGGAGTAAAC TGTAGATACG TTCTCTATCA CTGATAGGGA GTAAACTGGT CATACGTTCT CTATCACTGA TAGGGAGTAA ACTCCTTATA CGTTCTCTAT CACTGATAGG GAGTAAAGTC TGCATACGTT CTCTATCACT GATAGGGAGT AAACTCTTCA TACGTTCTCT ATCACTGATA GGGAGTAAAC TCGAGGTGAT AATTCCACGG GGTTGGGGTT GCGCCTTTTC CAAGGCAGCC CTGGGTTTGC GCAGGGACGC GGCTGCTCTG GGCGTGGTTC CGGGAAACGC AGCGGCGCCG ACCCTGGGTC TCGCACATTC TTCACGTCCG TTCGCAGCGT CACCCGGATC TTCGCCGCTA CCCTTGTGGG CCCCCCGGCG ACGCTTCCTG CTCCGCCCCT AAGTCGGGAA GGTTCCTTGC GGTTCGCGGC GTGCCGGACG TGACAAACGG AAGCCGCACG TCTCACTAGT ACCCTCGCAG ACGGACAGCG CCAGGGAGCA ATGGCAGCGC GCCGACCGCG ATGGGCTGTG GCCAATAGCG GCTGCTCAGC AGGGCGCGCC GAGAGCAGCG GCCGGGAAGG GGCGGTGCGG GAGGCGGGGT GTGGGGCGGT AGTGTGGGCC CTGTTCCTGC CCGCGCGGTG TTCCGCATTC TGCAAGCCTC CGGAGCGCAC GTCGGCAGTC GGCTCCCTCG TTGACCGAAT CACCGACCTC TCTCCCCAGG GGGATCATCG AATTACCATG TCTAGACTGG ACAAGAGCAA AGTCATAAAC TCTGCTCTGG AATTACTCAA TGGAGTCGGT ATCGAAGGCC TGACGACAAG GAAACTCGCT CAAAAGCTGG GAGTTGAGCA GCCTACCCTG TACTGGCACG TGAAGAACAA GCGGGCCCTG CTCGATGCCC TGCCAATCGA GATGCTGGAC AGGCATCATA CCCACTCCTG CCCCCTGGAA GGCGAGTCAT GGCAAGACTT TCTGCGGAAC AACGCCAAGT CATACCGCTG TGCTCTCCTC TCACATCGCG ACGGGGCTAA AGTGCATCTC GGCACCCGCC CAACAGAGAA ACAGTACGAA ACCCTGGAAA ATCAGCTCGC GTTCCTGTGT CAGCAAGGCT TCTCCCTGGA GAACGCACTG TACGCTCTGT CCGCCGTGGG CCACTTTACA CTGGGCTGCG TATTGGAGGA ACAGGAGCAT CAAGTAGCAA AAGAGGAAAG AGAGACACCT ACCACCGATT CTATGCCCCC ACTTCTGAAA CAAGCAATTG AGCTGTTCGA CCGGCAGGGA GCCGAACCTG CCTTCCTTTT CGGCCTGGAA CTAATCATAT GTGGCCTGGA GAAACAGCTA AAGTGCGAAA GCGGCGGGCC GACCGACGCC CTTGACGATT TTGACTTAGA CATGCTCCCA GCCGATGCCC TTGACGACTT TGACCTTGAT ATGCTGCCTG CTGACGCTCT TGACGATTTT GACCTTGACA TGCTCCCCGG GTAAACGCGC GAATCGCCCC TCTCCCTCCC CCCCCCCTAA CGTTACTGGC CGAAGCCGCT TGGAATAAGG CCGGTGTGCG TTTGTCTATA TGTTATTTTC CACCATATTG CCGTCTTTTG GCAATGTGAG GGCCCGGAAA CCTGGCCCTG TCTTCTTGAC GAGCATTCCT AGGGGTCTTT CCCCTCTCGC CAAAGGAATG CAAGGTCTGT TGAATGTCGT GAAGGAAGCA GTTCCTCTGG AAGCTTCTTG AAGACAAACA ACGTCTGTAG CGACCCTTTG CAGGCAGCGG AACCCCCCAC CTGGCGACAG GTGCCTCTGC GGCCAAAAGC CACGTGTATA AGATACACCT GCAAAGGCGG CACAACCCCA GTGCCACGTT GTGAGTTGGA TAGTTGTGGA AAGAGTCAAA TGGCTCTCCT CAAGCGTATT CAACAAGGGG CTGAAGGATG CCCAGAAGGT ACCCCATTGT ATGGGATCTG ATCTGGGGCC TCGGTGCACA TGCTTTACAT GTGTTTAGTC GAGGTTAAAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC GTGGTTTTCC TTTGAAAAAC ACGATGATAA GCTTGCCACA ACCCACAAGG AGACGACCTT CCATGACCGA GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCCGGGCCG TACGCACCCT CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGACCCGG ACCGCCACAT CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT AGTTTAAACC CGCTGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG GACGCGTCTG GAACAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAG CTGACGTCCT TTCCATGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCCTGGAAT TAATTCTGCA GTCGAGACCT AGAAAAACAT GGAGCAATCA CAAGTAGCAA TACAGCAGCT ACCAATGCTG ATTGTGCCTG GCTAGAAGCA CAAGAGGAGG AGGAGGTGGG TTTTCCAGTC ACACCTCAGG TACCTTTAAG ACCAATGACT TACAAGGCAG CTGTAGATCT TAGCCACTTT TTAAAAGAAA AGAGGGGACT GGAAGGGCTA ATTCACTCCC AACGAAGACA AGATATCCTT GATCTGTGGA TCTACCACAC ACAAGGCTAC TTCCCTGATT AGCAGAACTA CACACCAGGG CCAGGGGTCA GATATCCACT GACCTTTGGA TGGTGCTACA AGCTAGTACC AGTTGAGCCA GATAAGGTAG AAGAGGCCAA TAAAGGAGAG AACACCAGCT TGTTACACCC TGTGAGCCTG CATGGGATGG ATGACCCGGA GAGAGAAGTG TTAGAGTGGA GGTTTGACAG CCGCCTAGCA TTTCATCACG TGGCCCGAGA GCTGCATCCG GAGTACTTCA AGAACTGCTG ATATCGAGCT TGCTACAAGG GACTTTCCGC TGGGGACTTT CCAGGGAGGC GTGGCCTGGG CGGGACTGGG GAGTGGCGAG CCCTCAGATC CTGCATATAA GCAGCTGCTT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA GTCAGTGTGG AAAATCTCTA GCAGTAGTAG TTCATGTCAT CTTATTATTC AGTATTTATA ACTTGCAAAG AAATGAATAT CAGAGAGTGA GAGGCCTTGA CATTGCTAGC GTTTTACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCGACG GATCGGGAGA TCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGGATCAAC TGGATAACTC AAGCTAACCA AAATCATCCC AAACTTCCCA CCCCATACCC TATTACCACT GCCAATTACC TAGTGGTTTC ATTTACTCTA AACCTGTGAT TCCTCTGAAT TATTTTCATT TTAAAGAAAT TGTATTTGTT AAATATGTAC TACAAACTTA GTAGTTTTTA AAGAAATTGT ATTTGTTAAA TATGTACTAC AAACTTAGTA GT
