pDY1173_ADAR1p150
$99.00
In stock
SKU
PL37349
100μg liquid plasmid(Instantly):$120
| name | pDY1173_ADAR1p150 | another name | apDY1173_ADAR1p150 |
| Insert Size (bp) | 3681bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC CCACCATGAA GCTTATGAAT CCGCGGCAGG GGTATTCCCT CAGCGGATAC TACACCCATC CATTTCAAGG CTATGAGCAC AGACAGCTCA GATACCAGCA GCCTGGGCCA GGATCTTCCC CCAGTAGTTT CCTGCTTAAG CAAATAGAAT TTCTCAAGGG GCAGCTCCCA GAAGCACCGG TGATTGGAAA GCAGACACCG TCACTGCCAC CTTCCCTCCC AGGACTCCGG CCAAGGTTTC CAGTACTACT TGCCTCCAGT ACCAGAGGCA GGCAAGTGGA CATCAGGGGT GTCCCCAGGG GCGTGCATCT CGGAAGTCAG GGGCTCCAGA GAGGGTTCCA GCATCCTTCA CCACGTGGCA GGAGTCTGCC ACAGAGAGGT GTTGATTGCC TTTCCTCACA TTTCCAGGAA CTGAGTATCT ACCAAGATCA GGAACAAAGG ATCTTAAAGT TCCTGGAAGA GCTTGGGGAA GGGAAGGCCA CCACAGCACA TGATCTGTCT GGGAAACTTG GGACTCCGAA GAAAGAAATC AATCGAGTTT TATACTCCCT GGCAAAGAAG GGCAAGCTAC AGAAAGAGGC AGGAACACCC CCTTTGTGGA AAATCGCGGT CTCCACTCAG GCTTGGAACC AGCACAGCGG AGTGGTAAGA CCAGACGGTC ATAGCCAAGG AGCCCCAAAC TCAGACCCGA GTTTGGAACC GGAAGACAGA AACTCCACAT CTGTCTCAGA AGATCTTCTT GAGCCTTTTA TTGCAGTCTC AGCTCAGGCT TGGAACCAGC ACAGCGGAGT GGTAAGACCA GACAGTCATA GCCAAGGATC CCCAAACTCA GACCCAGGTT TGGAACCTGA AGACAGCAAC TCCACATCTG CCTTGGAAGA TCCTCTTGAG TTTTTAGACA TGGCCGAGAT CAAGGAGAAA ATCTGCGACT ATCTCTTCAA TGTGTCTGAC TCCTCTGCCC TGAATTTGGC TAAAAATATT GGCCTTACCA AGGCCCGAGA TATAAATGCT GTGCTAATTG ACATGGAAAG GCAGGGGGAT GTCTATAGAC AAGGGACAAC CCCTCCCATA TGGCATTTGA CAGACAAGAA GCGAGAGAGG ATGCAAATCA AGAGAAATAC GAACAGTGTT CCTGAAACCG CTCCAGCTGC AATCCCTGAG ACCAAAAGAA ACGCAGAGTT CCTCACCTGT AATATACCCA CATCAAATGC CTCAAATAAC ATGGTAACCA CAGAAAAAGT GGAGAATGGG CAGGAACCTG TCATAAAGTT AGAAAACAGG CAAGAGGCCA GACCAGAACC AGCAAGACTG AAACCACCTG TTCATTACAA TGGCCCCTCA AAAGCAGGGT ATGTTGACTT TGAAAATGGC CAGTGGGCCA CAGATGACAT CCCAGATGAC TTGAATAGTA TCCGCGCAGC ACCAGGTGAG TTTCGAGCCA TCATGGAGAT GCCCTCCTTC TACAGTCATG GCTTGCCACG GTGTTCACCC TACAAGAAAC TGACAGAGTG CCAGCTGAAG AACCCCATCA GCGGGCTGTT AGAATATGCC CAGTTCGCTA GTCAAACCTG TGAGTTCAAC ATGATAGAGC AGAGTGGACC ACCCCATGAA CCTCGATTTA AATTCCAGGT TGTCATCAAT GGCCGAGAGT TTCCCCCAGC TGAAGCTGGA AGCAAGAAAG TGGCCAAGCA GGATGCAGCT ATGAAAGCCA TGACAATTCT GCTAGAGGAA GCCAAAGCCA AGGACAGTGG AAAATCAGAA GAATCATCCC ACTATTCCAC AGAGAAAGAA TCAGAGAAGA CTGCAGAGTC CCAGACCCCC ACCCCTTCAG CCACATCCTT CTTTTCTGGG AAGAGCCCCG TCACCACACT GCTTGAGTGT ATGCACAAAT TGGGGAACTC CTGCGAATTC CGTCTCCTGT CCAAAGAAGG CCCTGCCCAT GAACCCAAGT TCCAATACTG TGTTGCAGTG GGAGCCCAAA CTTTCCCCAG TGTGAGTGCT CCCAGCAAGA AAGTGGCAAA GCAGATGGCC GCAGAGGAAG CCATGAAGGC CCTGCATGGG GAGGCGACCA ACTCCATGGC TTCTGATAAC CAGCCTGAAG GTATGATCTC AGAGTCACTT GATAACTTGG AATCCATGAT GCCCAACAAG GTCAGGAAGA TTGGCGAGCT CGTGAGATAC CTGAACACCA ACCCTGTGGG TGGCCTTTTG GAGTACGCCC GCTCCCATGG CTTTGCTGCT GAATTCAAGT TGGTCGACCA GTCCGGACCT CCTCACGAGC CCAAGTTCGT TTACCAAGCA AAAGTTGGGG GTCGCTGGTT CCCAGCCGTC TGCGCACACA GCAAGAAGCA AGGCAAGCAG GAAGCAGCAG ATGCGGCTCT CCGTGTCTTG ATTGGGGAGA ACGAGAAGGC AGAACGCATG GGTTTCACAG AGGTAACCCC AGTGACAGGG GCCAGTCTCA GAAGAACTAT GCTCCTCCTC TCAAGGTCCC CAGAAGCACA GCCAAAGACA CTCCCTCTCA CTGGCAGCAC CTTCCATGAC CAGATAGCCA TGCTGAGCCA CCGGTGCTTC AACACTCTGA CTAACAGCTT CCAGCCCTCC TTGCTCGGCC GCAAGATTCT GGCCGCCATC ATTATGAAAA AAGACTCTGA GGACATGGGT GTCGTCGTCA GCTTGGGAAC AGGGAATCGC TGTGTAAAAG GAGATTCTCT CAGCCTAAAA GGAGAAACTG TCAATGACTG CCATGCAGAA ATAATCTCCC GGAGAGGCTT CATCAGGTTT CTCTACAGTG AGTTAATGAA ATACAACTCC CAGACTGCGA AGGATAGTAT ATTTGAACCT GCTAAGGGAG GAGAAAAGCT CCAAATAAAA AAGACTGTGT CATTCCATCT GTATATCAGC ACTGCTCCGT GTGGAGATGG CGCCCTCTTT GACAAGTCCT GCAGCGACCG TGCTATGGAA AGCACAGAAT CCCGCCACTA CCCTGTCTTC GAGAATCCCA AACAAGGAAA GCTCCGCACC AAGGTGGAGA ACGGAGAAGG CACAATCCCT GTGGAATCCA GTGACATTGT GCCTACGTGG GATGGCATTC GGCTCGGGGA GAGACTCCGT ACCATGTCCT GTAGTGACAA AATCCTACGC TGGAACGTGC TGGGCCTGCA AGGGGCACTG TTGACCCACT TCCTGCAGCC CATTTATCTC AAATCTGTCA CATTGGGTTA CCTTTTCAGC CAAGGGCATC TGACCCGTGC TATTTGCTGT CGTGTGACAA GAGATGGGAG TGCATTTGAG GATGGACTAC GACATCCCTT TATTGTCAAC CACCCCAAGG TTGGCAGAGT CAGCATATAT GATTCCAAAA GGCAATCCGG GAAGACTAAG GAGACAAGCG TCAACTGGTG TCTGGCTGAT GGCTATGACC TGGAGATCCT GGACGGTACC AGAGGCACTG TGGATGGGCC ACGGAATGAA TTGTCCCGGG TCTCCAAAAA GAACATTTTT CTTCTATTTA AGAAGCTCTG CTCCTTCCGT TACCGCAGGG ATCTACTGAG ACTCTCCTAT GGTGAGGCCA AGAAAGCTGC CCGTGACTAC GAGACGGCCA AGAACTACTT CAAAAAAGGC CTGAAGGATA TGGGCTATGG GAACTGGATT AGCAAACCCC AGGAGGAAAA GAACTTTTAT CTCTGCCCAG TATAGTCTAG AGGGCCCGTT TAAACCCGCT GATCAGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAC AATAGCAGGC ATGCTGGGGA TGCGGTGGGC TCTATGGCTT CTGAGGCGGA AAGAACCAGC TGGGGCTCTA GGGGGTATCC CCACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TAATTCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCGACG GATCGGGAGA TCTCCCGATC CCCTATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTAT
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