pCMV-mCherry-Nlrp12(rat)-Neo
$99.00
In stock
SKU
PL37343
NM_001169142.1;Nalp12
100μg liquid plasmid(Instantly):$120
| name | pCMV-mCherry-Nlrp12(rat)-Neo | another name | apCMV-mCherry-Nlrp12(rat)-Neo |
| Insert Size (bp) | 3165bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Rat | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | Red |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGCT ACCGGTCGCC ACCATGGTGA GCAAGGGCGA GGAGGATAAC ATGGCCATCA TCAAGGAGTT CATGCGCTTC AAGGTGCACA TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG TGGCCCCCTG CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCATGTACG GCTCCAAGGC CTACGTGAAG CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG GACGGCGAGT TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCTCCG AGCGGATGTA CCCCGAGGAC GGCGCCCTGA AGGGCGAGAT CAAGCAGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC GCTGAGGTCA AGACCACCTA CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACAAC GTCAACATCA AGTTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC GAACGCGCCG AGGGCCGCCA CTCCACCGGC GGCATGGACG AGCTGTACAA GTCCGGACTC AGATCTCGAG GAGGTGGAGG TTCAATGTTG CCAGCTACAG CCAAGGATGC CCTCCATCGA CTGTCCACCT ACCTGGAAGA ACTTGAGGCT GGGGAACTGA AGAAATTCAA ATTATACCTG GGGATTGCGG AGGAACTGGG CCAGGACAAG ATCCCCAGGG GACGAATGGA GATGGCTGGT CCTCTGGAAA TGGCCCAATT GATGGTGGCC CATATGGGGA CAAAGGAGGC TTGGCTTCTG GCTCTCAGCA CCTTTGAGAG GATCCACAGG AAGGACCTGT GGGAGCGAGG ACAGGGAGAA GACCTGGTGA GGGTGACTCC AAGTAATGGT CTATGCTCTC TTGAGAGCCA GTCAACGTGC CTTTCGGACG TCTCTCCTGA TGCTCCAAGA AAAGATCCCC AGATAACCTA CAAAGACTAT GTCCGAAGGA AATTCCGGCT AATGGAAGAC CGCAATGCGC GACTAGGCGA ATGTGTGAAC TTAAGTCATC GTTACACTCG GCTTCTCCTA GTAAAGGAAC ACTCAAATCC TATCTGGGCA CAGCAAAAAC TTCTGGAAAC AGGGTGGGAA CACTCCAGAA CCAGGGGCCA CCAGGCTAGC CCTATCCAAA TGGAGACCCT CTTTGAGCCA GATGAAGAAC GCCCGGAGCC ACCACGCACA GTGGTATTAC AAGGGGCAGC GGGGATGGGG AAGTCCATGT TGACCCACAA AGTGATGTTG GACTGGGCCG ATGGGAGACT CTTCCAAGAC CAGTTCGATT ATGTCTTCTA TATCAGCTGC AGGGAGTTGA ATAGAAGCCA CACCCAGTGC AGTGTACATG ACCTCCTCTC CAGCTGCTGG CCAGAGCATG GTGCACCCCT CGAGGACCTC ATCCGGGCTC CTGATCGTCT TCTATTCATC ATCGATGGCT TCCATGAGCT TCATCCCTCT TTCCATGACG TTCAGGGTCC CTGGTGCCAC TGCTGGGAGG AGAAACGACC CACGGAACTC CTCCTCGGAA GCCTGATTCG GAGGTTGCTT CTGCCCCAGC TCTCTTTGCT CATCACCACA CGACCCTGTG CACTGGAGAA GCTGCACGGC TTGTTGGAAC ACCCTAGGCA CGTGGAGATC CTGGGCTTCT CCGAGGCAGA GAGGGAGGAA TATTTCTACA GATATTTCCA CAACACTGGA CAAGCGAGCC AAGTGTTCAG CTTCATGAGG GACTATGAGC CCCTCTTTAC CATGTGTTTT GTTCCCATGG TGTCCTGGGT GGTCTGCACC TGCTTAAAGC AGCAGCTAGA AAGTGGGGAG CTTTTAAGAC AAACATCTAG GACCACCACA GCGGTTTATA TGTTCTACCT TCTGAGCCTG ATGCAGCCCA AGCCAGGGAC CCCAACCTTC AAAGTCCCAG CCAACCAGAG AGGCCTGGTC TCTCTGGCTG CAGAGGGCCT CTGGAATCAG AAGATTCTCT TCGAGGAAGA GGATCTTGGG AAACATGGCC TAGATGGAGC AGAGGTCTCC ACCTTCCTCA ACGTGAACAT ATTCCAGAAG GGCATCAAGT GTGAGAAATT CTACAGCTTC ATCCACCTGA GTTTCCAGGA ATTCTTCACA GCCATGTACT GTGCACTGCA CGGCAGAGAG GCGGTGAGGA GAGCACTGGC TGAGTATGGT TTCTCAGAAA GGAACTTCTT GGCCCACACC GTCCGCTTTC TCTTTGGCCT CCTCAATGAA GAGATGAGAT GCTACCTCGA GAGGAATCTC GGCTGGACCA TCTCCCCTCA GGTCAAGGAG GAAGCATTGG CGTGGATCCA AAACAAGGCT CGAAGTGAGG GCTCCACCCT GCAGCATGGC TCCCTGGAGC TTCTCAGCTG CTTGTACGAG ATCCAGGAGG AAGACTTCAT CCAGCAGGCC CTGAGCCACT TTCAGGTGGT TGTTGTCAGA AACCTCTCAA CGAAGATGGA GCACGTGGTC TGCTCGTTTT GTGCGAGGTA CTGCAGAGGC ACAGAAGTGC TTCACTTGTA TGGGAGTGCC TATAGTACAG GCGCAGAGGA TGGCCCGCCA GAACCTCCAG GAGCCCAGAC GCAGTCCACA CACTCACAGG AAAGGAACAT CCTTCCTGAC ATTTACAGTG CATACCTCTC AGCAACCATC TGCACCAACT CAAACCTGAT CGAGCTGGCC TTGTATCGAA ACGCCTTGGG CAGCCAGGGC GTGAGGCTGC TCTGTCAAGG CCTCCGACAT GCAAACTGCA AGCTTCAGAA CCTGAGGCTG AAGAGGTGTC ACATCTCCGG ATCAGCCTGC CAGGATCTCG CAGCGGCTAT CATCGCCAAT AGGAATTTAA TCAGGCTGGA CCTCAGTGGC AACAGCATTG GAGTGCTAGG CCTAGAGCTG CTCTGTGAGG GGTTACAGCA CCCCATGTGT AGGCTGCAGA TGATCCAGCT GAGGAAGTGT TTGCTGGAGG CTGCGGCTGG CCGAGCACTG GCCTCTGTTC TCAGCAACAA CTCACATCTT GTAGAACTGG ATCTGACAGG AAACCCCTTG GAGGATTTGG GGCTGAAGTT ACTGTGTCAA GGGCTGAGGC ACCCAGTCTG CAGGCTGCGG ACCTTGTGGC TGAAGATCTG CCACCTTGGA CAAGCTTCCT GTGAAGATCT GGCTTCTACT CTCAAAATGA ACCAGAGCCT GATGGAGCTG GACCTGGGTC TGAATGACCT TGGGGATTCT GGGGCGCTTC TGCTGTGTGA GGGCCTCAGG CATCCAGATT GCAAACTCCA GACCCTCCGG CTGGGCATTT GCCGACTGGG CTCAGACGCG TGTGCAGGGG TTGCTAGTGT GCTCCAGGTC AACACGTGCC TCCGAGAGCT GGACCTGAGC TTCAATGACT TGGGAGACAG GGGCCTGTGG CTGCTGGGGG AAGGACTGAG GCACCAGACC TGCAGACTCC AGAAGCTCTG GCTGGACAGT TGCGGCCTCA CTTCCAAAGC ATGTGAGGAC CTGTCTTCAG TCCTGGGAAT CAGTCAGACC CTGAATGAGC TTTATCTGAC CAATAATGCT CTGGGGGACA CTGGTGTTCG TCTGCTGTGC AAGAGGCTGA GGCATCCGGG GTGCAAGCTT CGTGTCCTGT GGCTTTTCGG AATGGACCTG AACAAAGTGA CCCACAGGAG GATGGCGGCA CTTCGAGTCA CAAAACCATA CCTAGATATT GGGTGCTAAG GATCCTCTAG AGGGCCCGTT TAAACCCGCT GATCAGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAC AATAGCAGGC ATGCTGGGGA TGCGGTGGGC TCTATGGCTT CTGAGGCGGA AAGAACCAGC TGGGGCTCTA GGGGGTATCC CCACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TAATTCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGT
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