pCMV-HDAC5(human)-3×FLAG-Neo
$99.00
In stock
SKU
PL37241
NM_001015053.2;HD5; NY-CO-9
100μg liquid plasmid(Instantly):$120
| name | pCMV-HDAC5(human)-3×FLAG-Neo | another name | apCMV-HDAC5(human)-3×FLAG-Neo |
| Insert Size (bp) | 3369bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCATGAA CTCTCCCAAC GAGTCGGCAG ATGGGATGTC AGGTCGGGAA CCATCCTTGG AAATCCTGCC GCGGACTTCT CTGCACAGCA TCCCTGTGAC AGTGGAGGTG AAGCCGGTGC TGCCAAGAGC CATGCCCAGT TCCATGGGGG GTGGGGGTGG AGGCAGCCCC AGCCCTGTGG AGCTACGGGG GGCTCTGGTG GGCTCTGTGG ACCCCACACT GCGGGAGCAG CAACTGCAGC AGGAGCTCCT GGCGCTCAAG CAGCAGCAGC AGCTGCAGAA GCAGCTCCTG TTCGCTGAGT TCCAGAAACA GCATGACCAC CTGACAAGGC AGCATGAGGT CCAGCTGCAG AAGCACCTCA AGCAGCAGCA GGAGATGCTG GCAGCCAAGC AGCAGCAGGA GATGCTGGCA GCCAAGCGGC AGCAGGAGCT GGAGCAGCAG CGGCAGCGGG AGCAGCAGCG GCAGGAAGAG CTGGAGAAGC AGCGGCTGGA GCAGCAGCTG CTCATCCTGC GGAACAAGGA GAAGAGCAAA GAGAGTGCCA TTGCCAGCAC TGAGGTAAAG CTGAGGCTCC AGGAATTCCT CTTGTCGAAG TCAAAGGAGC CCACACCAGG CGGCCTCAAC CATTCCCTCC CACAGCACCC CAAATGCTGG GGAGCCCACC ATGCTTCTTT GGACCAGAGT TCCCCTCCCC AGAGCGGCCC CCCTGGGACG CCTCCCTCCT ACAAACTGCC TTTGCCTGGG CCCTACGACA GTCGAGACGA CTTCCCCCTC CGCAAAACAG CCTCTGAACC CAACTTGAAA GTGCGTTCAA GGCTAAAACA GAAGGTGGCT GAGCGGAGAA GCAGTCCCCT CCTGCGTCGC AAGGATGGGA CTGTTATTAG CACCTTTAAG AAGAGAGCTG TTGAGATCAC AGGTGCCGGG CCTGGGGCGT CGTCCGTGTG TAACAGCGCA CCCGGCTCCG GCCCCAGCTC TCCCAACAGC TCCCACAGCA CCATCGCTGA GAATGGCTTT ACTGGCTCAG TCCCCAACAT CCCCACTGAG ATGCTCCCTC AGCACCGAGC CCTCCCTCTG GACAGCTCCC CCAACCAGTT CAGCCTCTAC ACGTCTCCTT CTCTGCCCAA CATCTCCCTA GGGCTGCAGG CCACGGTCAC TGTCACCAAC TCACACCTCA CTGCCTCCCC GAAGCTGTCG ACACAGCAGG AGGCCGAGAG GCAGGCCCTC CAGTCCCTGC GGCAGGGTGG CACGCTGACC GGCAAGTTCA TGAGCACATC CTCTATTCCT GGCTGCCTGC TGGGCGTGGC ACTGGAGGGC GACGGGAGCC CCCACGGGCA TGCCTCCCTG CTGCAGCATG TGCTGTTGCT GGAGCAGGCC CGGCAGCAGA GCACCCTCAT TGCTGTGCCA CTCCACGGGC AGTCCCCACT AGTGACGGGT GAACGTGTGG CCACCAGCAT GCGGACGGTA GGCAAGCTCC CGCGGCATCG GCCCCTGAGC CGCACTCAGT CCTCACCGCT GCCGCAGAGT CCCCAGGCCC TGCAGCAGCT GGTCATGCAA CAACAGCACC AGCAGTTCCT GGAGAAGCAG AAGCAGCAGC AGCTACAGCT GGGCAAGATC CTCACCAAGA CAGGGGAGCT GCCCAGGCAG CCCACCACCC ACCCTGAGGA GACAGAGGAG GAGCTGACGG AGCAGCAGGA GGTCTTGCTG GGGGAGGGAG CCCTGACCAT GCCCCGGGAG GGCTCCACAG AGAGTGAGAG CACACAGGAA GACCTGGAGG AGGAGGACGA GGAAGACGAT GGGGAGGAGG AGGAGGATTG CATCCAGGTT AAGGACGAGG AGGGCGAGAG TGGTGCTGAG GAGGGGCCCG ACTTGGAGGA GCCTGGTGCT GGATACAAAA AACTGTTCTC AGATGCCCAG CCGCTGCAGC CTTTGCAGGT GTACCAGGCG CCCCTCAGCC TGGCCACTGT GCCCCACCAG GCCCTGGGCC GTACCCAGTC CTCCCCTGCT GCCCCTGGGG GCATGAAGAG CCCCCCAGAC CAGCCCGTCA AGCACCTCTT CACCACAGGT GTGGTCTACG ACACGTTCAT GCTAAAGCAC CAGTGCATGT GCGGGAACAC ACACGTGCAC CCTGAGCATG CTGGCCGGAT CCAGAGCATC TGGTCCCGGC TGCAGGAGAC AGGCCTGCTT AGCAAGTGCG AGCGGATCCG AGGTCGCAAA GCCACGCTAG ATGAGATCCA GACAGTGCAC TCTGAATACC ACACCCTGCT CTATGGGACC AGTCCCCTCA ACCGGCAGAA GCTAGACAGC AAGAAGTTGC TCGGCCCCAT CAGCCAGAAG ATGTATGCTG TGCTGCCTTG TGGGGGCATC GGGGTGGACA GTGACACCGT GTGGAATGAG ATGCACTCCT CCAGTGCTGT GCGCATGGCA GTGGGCTGCC TGCTGGAGCT GGCCTTCAAG GTGGCTGCAG GAGAGCTCAA GAATGGATTT GCCATCATCC GGCCCCCAGG ACACCACGCC GAGGAATCCA CAGCCATGGG ATTCTGCTTC TTCAACTCTG TAGCCATCAC CGCAAAACTC CTACAGCAGA AGTTGAACGT GGGCAAGGTC CTCATCGTGG ACTGGGACAT TCACCATGGC AATGGCACCC AGCAGGCGTT CTACAATGAC CCCTCTGTGC TCTACATCTC TCTGCATCGC TATGACAACG GGAACTTCTT TCCAGGCTCT GGGGCTCCTG AAGAGGTTGG TGGAGGACCA GGCGTGGGGT ACAATGTGAA CGTGGCATGG ACAGGAGGTG TGGACCCCCC CATTGGAGAC GTGGAGTACC TTACAGCCTT CAGGACAGTG GTGATGCCCA TTGCCCACGA GTTCTCACCT GATGTGGTCC TAGTCTCCGC CGGGTTTGAT GCTGTTGAAG GACATCTGTC TCCTCTGGGT GGCTACTCTG TCACCGCCAG ATGTTTTGGC CACTTGACCA GGCAGCTGAT GACCCTGGCA GGGGGCCGGG TGGTGCTGGC CCTGGAGGGA GGCCATGACT TGACCGCCAT CTGTGATGCC TCTGAGGCTT GTGTCTCGGC TCTGCTCAGT GTAGAGCTGC AGCCCTTGGA TGAGGCAGTC TTGCAGCAAA AGCCCAACAT CAACGCAGTG GCCACGCTAG AGAAAGTCAT CGAGATCCAG AGCAAACACT GGAGCTGTGT GCAGAAGTTC GCCGCTGGTC TGGGCCGGTC CCTGCGAGAG GCCCAAGCAG GTGAGACCGA GGAGGCCGAG ACTGTGAGCG CCATGGCCTT GCTGTCGGTG GGGGCCGAGC AGGCCCAGGC TGCGGCAGCC CGGGAACACA GCCCCAGGCC GGCAGAGGAG CCCATGGAGC AGGAGCCTGC CCTGGGATCC GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT GACAAGTAGT GAGGGCCCGA TATCTCGACA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAGCTGAC GTCCTTTCCA TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCCT GGAAACGGGG GAGGCTAACT GAAACACGGA AGGAGACAAT ACCGGAAGGA ACCCGCGCTA TGACGGCAAT AAAAAGACAG AATAAAACGC ACGGGTGTTG GGTCGTTTGT TCATAAACGC GGGGTTCGGT CCCAGGGCTG GCACTCTGTC GATACCCCAC CGAGACCCCA TTGGGGCCAA TACGCCCGCG TTTCTTCCTT TTCCCCACCC CACCCCCCAA GTTCGGGTGA AGGCCCAGGG CTCGCAGCCA ACGTCGGGGC GGCAGGCCCT GCCATAGCAG ATCTGCGCAG CTGGGGCTCT AGGGGGTATC CCCACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG CATCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGGGGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTAATTCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCAAT GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCG ACGGATCGGG AGATCTCCCG ATCCCCTATG GTCGACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATC TGCTCCCTGC TTGTGTGTTG GAGGTCGCTG AGTAGTGCGC GAGCAAAATT TAAGCTACAA CAAGGCAAGG CTTGACCGAC AATTGCATGA AGAATCTGCT TAGG
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