NM_000057.4;BS; MGRISCE1; RECQ2; RECQL2; RECQL3
100μg liquid plasmid(Instantly):$134
| name | pLV3-CMV-BLM(human)-3×FLAG-CopGFP-Puro | another name | apLV3-CMV-BLM(human)-3×FLAG-CopGFP-Puro |
| Insert Size (bp) | 4251bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT TATCGATACT AGTATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTTT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGATTC TAGAGCTAGC CTGATACGAA CTCGGAATTC GCCACCATGG CTGCTGTTCC TCAAAATAAT CTACAGGAGC AACTAGAACG TCACTCAGCC AGAACACTTA ATAATAAATT AAGTCTTTCA AAACCAAAAT TTTCAGGTTT CACTTTTAAA AAGAAAACAT CTTCAGATAA CAATGTATCT GTAACTAATG TGTCAGTAGC AAAAACACCT GTATTAAGAA ATAAAGATGT TAATGTTACC GAAGACTTTT CCTTCAGTGA ACCTCTACCC AACACCACAA ATCAGCAAAG GGTCAAGGAC TTCTTTAAAA ATGCTCCAGC AGGACAGGAA ACACAGAGAG GTGGATCAAA ATCATTATTG CCAGATTTCT TGCAGACTCC GAAGGAAGTT GTATGCACTA CCCAAAACAC ACCAACTGTA AAGAAATCCC GGGATACTGC TCTCAAGAAA TTAGAATTTA GTTCTTCACC AGATTCTTTA AGTACCATCA ATGATTGGGA TGATATGGAT GACTTTGATA CTTCTGAGAC TTCAAAATCA TTTGTTACAC CACCCCAAAG TCACTTTGTA AGAGTAAGCA CTGCTCAGAA ATCAAAAAAG GGTAAGAGAA ACTTTTTTAA AGCACAGCTT TATACAACAA ACACAGTAAA GACTGATTTG CCTCCACCCT CCTCTGAAAG CGAGCAAATA GATTTGACTG AGGAACAGAA GGATGACTCA GAATGGTTAA GCAGCGATGT GATTTGCATC GATGATGGCC CCATTGCTGA AGTGCATATA AATGAAGATG CTCAGGAAAG TGACTCTCTG AAAACTCATT TGGAAGATGA AAGAGATAAT AGCGAAAAGA AGAAGAATTT GGAAGAAGCT GAATTACATT CAACTGAGAA AGTTCCATGT ATTGAATTTG ATGATGATGA TTATGATACG GATTTTGTTC CACCTTCTCC AGAAGAAATT ATTTCTGCTT CTTCTTCCTC TTCAAAATGC CTTAGTACGT TAAAGGACCT TGACACCTCT GACAGAAAAG AGGATGTTCT TAGCACATCA AAAGATCTTT TGTCAAAACC TGAGAAAATG AGTATGCAGG AGCTGAATCC AGAAACCAGC ACAGACTGTG ACGCTAGACA GATAAGTTTA CAGCAGCAGC TTATTCATGT GATGGAGCAC ATCTGTAAAT TAATTGATAC TATTCCTGAT GATAAACTGA AACTTTTGGA TTGTGGGAAC GAACTGCTTC AGCAGCGGAA CATAAGAAGG AAACTTCTAA CGGAAGTAGA TTTTAATAAA AGTGATGCCA GTCTTCTTGG CTCATTGTGG AGATACAGGC CTGATTCACT TGATGGCCCT ATGGAGGGTG ATTCCTGCCC TACAGGGAAT TCTATGAAGG AGTTAAATTT TTCACACCTT CCCTCAAATT CTGTTTCTCC TGGGGACTGT TTACTGACTA CCACCCTAGG AAAGACAGGA TTCTCTGCCA CCAGGAAGAA TCTTTTTGAA AGGCCTTTAT TCAATACCCA TTTACAGAAG TCCTTTGTAA GTAGCAACTG GGCTGAAACA CCAAGACTAG GAAAAAAAAA TGAAAGCTCT TATTTCCCAG GAAATGTTCT CACAAGCACT GCTGTGAAAG ATCAGAATAA ACATACTGCT TCAATAAATG ACTTAGAAAG AGAAACCCAA CCTTCCTATG ATATTGATAA TTTTGACATA GATGACTTTG ATGATGATGA TGACTGGGAA GACATAATGC ATAATTTAGC AGCCAGCAAA TCTTCCACAG CTGCCTATCA ACCCATCAAG GAAGGTCGGC CAATTAAATC AGTATCAGAA AGACTTTCCT CAGCCAAGAC AGACTGTCTT CCAGTGTCAT CTACTGCTCA AAATATAAAC TTCTCAGAGT CAATTCAGAA TTATACTGAC AAGTCAGCAC AAAATTTAGC ATCCAGAAAT CTGAAACATG AGCGTTTCCA AAGTCTTAGT TTTCCTCATA CAAAGGAAAT GATGAAGATT TTTCATAAAA AATTTGGCCT GCATAATTTT AGAACTAATC AGCTAGAGGC GATCAATGCT GCACTGCTTG GTGAAGACTG TTTTATCCTG ATGCCGACTG GAGGTGGTAA GAGTTTGTGT TACCAGCTCC CTGCCTGTGT TTCTCCTGGG GTCACTGTTG TCATTTCTCC CTTGAGATCA CTTATCGTAG ATCAAGTCCA AAAGCTGACT TCCTTGGATA TTCCAGCTAC ATATCTGACA GGTGATAAGA CTGACTCAGA AGCTACAAAT ATTTACCTCC AGTTATCAAA AAAAGACCCA ATCATAAAAC TTCTATATGT CACTCCAGAA AAGATCTGTG CAAGTAACAG ACTCATTTCT ACTCTGGAGA ATCTCTATGA GAGGAAGCTC TTGGCACGTT TTGTTATTGA TGAAGCACAT TGTGTCAGTC AGTGGGGACA TGATTTTCGT CAAGATTACA AAAGAATGAA TATGCTTCGC CAGAAGTTTC CTTCTGTTCC GGTGATGGCT CTTACGGCCA CAGCTAATCC CAGGGTACAG AAGGACATCC TGACTCAGCT GAAGATTCTC AGACCTCAGG TGTTTAGCAT GAGCTTTAAC AGACATAATC TGAAATACTA TGTATTACCG AAAAAGCCTA AAAAGGTGGC ATTTGATTGC CTAGAATGGA TCAGAAAGCA CCACCCATAT GATTCAGGGA TAATTTACTG CCTCTCCAGG CGAGAATGTG ACACCATGGC TGACACGTTA CAGAGAGATG GGCTCGCTGC TCTTGCTTAC CATGCTGGCC TCAGTGATTC TGCCAGAGAT GAAGTGCAGC AGAAGTGGAT TAATCAGGAT GGCTGTCAGG TTATCTGTGC TACAATTGCA TTTGGAATGG GGATTGACAA ACCGGACGTG CGATTTGTGA TTCATGCATC TCTCCCTAAA TCTGTGGAGG GTTACTACCA AGAATCTGGC AGAGCTGGAA GAGATGGGGA AATATCTCAC TGCCTGCTTT TCTATACCTA TCATGATGTG ACCAGACTGA AAAGACTTAT AATGATGGAA AAAGATGGAA ACCATCATAC AAGAGAAACT CACTTCAATA ATTTGTATAG CATGGTACAT TACTGTGAAA ATATAACGGA ATGCAGGAGA ATACAGCTTT TGGCCTACTT TGGTGAAAAT GGATTTAATC CTGATTTTTG TAAGAAACAC CCAGATGTTT CTTGTGATAA TTGCTGTAAA ACAAAGGATT ATAAAACAAG AGATGTGACT GACGATGTGA AAAGTATTGT AAGATTTGTT CAAGAACATA GTTCATCACA AGGAATGAGA AATATAAAAC ATGTAGGTCC TTCTGGAAGA TTTACTATGA ATATGCTGGT CGACATTTTC TTGGGGAGTA AGAGTGCAAA AATCCAGTCA GGTATATTTG GAAAAGGATC TGCTTATTCA CGACACAATG CCGAAAGACT TTTTAAAAAG CTGATACTTG ACAAGATTTT GGATGAAGAC TTATATATCA ATGCCAATGA CCAGGCGATC GCTTATGTGA TGCTCGGAAA TAAAGCCCAA ACTGTACTAA ATGGCAATTT AAAGGTAGAC TTTATGGAAA CAGAAAATTC CAGCAGTGTG AAAAAACAAA AAGCGTTAGT AGCAAAAGTG TCTCAGAGGG AAGAGATGGT TAAAAAATGT CTTGGAGAAC TTACAGAAGT CTGCAAATCT CTGGGGAAAG TTTTTGGTGT CCATTACTTC AATATTTTTA ATACCGTCAC TCTCAAGAAG CTTGCAGAAT CTTTATCTTC TGATCCTGAG GTTTTGCTTC AAATTGATGG TGTTACTGAA GACAAACTGG AAAAATATGG TGCGGAAGTG ATTTCAGTAT TACAGAAATA CTCTGAATGG ACATCGCCAG CTGAAGACAG TTCCCCAGGG ATAAGCCTGT CCAGCAGCAG AGGCCCCGGA AGAAGTGCCG CTGAGGAGCT CGACGAGGAA ATACCCGTAT CTTCCCACTA CTTTGCAAGT AAAACCAGAA ATGAAAGGAA GAGGAAAAAG ATGCCAGCCT CCCAAAGGTC TAAGAGGAGA AAAACTGCTT CCAGTGGTTC CAAGGCAAAG GGGGGGTCTG CCACATGTAG AAAGATATCT TCCAAAACGA AATCCTCCAG CATCATTGGA TCCAGTTCAG CCTCACATAC TTCTCAAGCG ACATCAGGAG CCAATAGCAA ATTGGGGATT ATGGCTCCAC CGAAGCCTAT AAATAGACCG TTTCTTAAGC CTTCATATGC ATTCTCAGGA TCCGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG ACATCGACTA CAAGGATGAC GATGACAAGT AGCGGCCGCG AAGGATCTGC GATCGCTCCG GTGCCCGTCA GTGGGCAGAG CGCACATCGC CCACAGTCCC CGAGAAGTTG GGGGGAGGGG TCGGCAATTG AACGGGTGCC TAGAGAAGGT GGCGCGGGGT AAACTGGGAA AGTGATGTCG TGTACTGGCT CCGCCTTTTT CCCGAGGGTG GGGGAGAACC GTATATAAGT GCAGTAGTCG CCGTGAACGT TCTTTTTCGC AACGGGTTTG CCGCCAGAAC ACAGCTGAAG CTTCGAGGGG CTCGCATCTC TCCTTCACGC GCCCGCCGCC CTACCTGAGG CCGCCATCCA CGCCGGTTGA GTCGCGTTCT GCCGCCTCCC GCCTGTGGTG CCTCCTGAAC TGCGTCCGCC GTCTAGGTAA GTTTAAAGCT CAGGTCGAGA CCGGGCCTTT GTCCGGCGCT CCCTTGGAGC CTACCTAGAC TCAGCCGGCT CTCCACGCTT TGCCTGACCC TGCTTGCTCA ACTCTACGTC TTTGTTTCGT TTTCTGTTCT GCGCCGTTAC AGATCCAAGC TGTGACCGGC GCCTACGCTA GACGCCACCA TGGAGAGCGA CGAGAGCGGC CTGCCCGCCA TGGAGATCGA GTGCCGCATC ACCGGCACCC TGAACGGCGT GGAGTTCGAG CTGGTGGGCG GCGGAGAGGG CACCCCCAAG CAGGGCCGCA TGACCAACAA GATGAAGAGC ACCAAAGGCG CCCTGACCTT CAGCCCCTAC CTGCTGAGCC ACGTGATGGG CTACGGCTTC TACCACTTCG GCACCTACCC CAGCGGCTAC GAGAACCCCT TCCTGCACGC CATCAACAAC GGCGGCTACA CCAACACCCG CATCGAGAAG TACGAGGACG GCGGCGTGCT GCACGTGAGC TTCAGCTACC GCTACGAGGC CGGCCGCGTG ATCGGCGACT TCAAGGTGGT GGGCACCGGC TTCCCCGAGG ACAGCGTGAT CTTCACCGAC AAGATCATCC GCAGCAACGC CACCGTGGAG CACCTGCACC CCATGGGCGA TAACGTGCTG GTGGGCAGCT TCGCCCGCAC CTTCAGCCTG CGCGACGGCG GCTACTACAG CTTCGTGGTG GACAGCCACA TGCACTTCAA GAGCGCCATC CACCCCAGCA TCCTGCAGAA CGGGGGCCCC ATGTTCGCCT TCCGCCGCGT GGAGGAGCTG CACAGCAACA CCGAGCTGGG CATCGTGGAG TACCAGCACG CCTTCAAGAC CCCCATCGCC TTCGCCAGAT CCCGCGCTCA GTCGTCCAAT TCTGCCGTGG ACGGCACCGC CGGACCCGGC TCCACCGGAT CTCGCGAGGG CAGAGGAAGT CTTCTAACAT GCGGTGACGT GGAGGAGAAT CCCGGCCCTA TGACCGAGTA CAAGCCCACG GTGCGCCTCG CCACCCGCGA CGACGTCCCC AGGGCCGTAC GCACCCTCGC CGCCGCGTTC GCCGACTACC CCGCCACGCG CCACACCGTC GATCCGGACC GCCACATCGA GCGGGTCACC GAGCTGCAAG AACTCTTCCT CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG GACGACGGCG CCGCGGTGGC GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG TTCGCCGAGA TCGGCCCGCG CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA CAGATGGAAG GCCTCCTGGC GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC GTCGGCGTCT CGCCCGACCA CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA GTGGAGGCGG CCGAGCGCGC CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCGCAAC CTCCCCTTCT ACGAGCGGCT CGGCTTCACC GTCACCGCCG ACGTCGAGGT GCCCGAAGGA CCGCGCACCT GGTGCATGAC CCGCAAGCCC GGTGCCTGAA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC GTCCTTTCCT TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCCT GGTACCTTTA AGACCAATGA CTTACAAGGC AGCTGTAGAT CTTAGCCACT TTTTAAAAGA AAAGGGGGGA CTGGAAGGGC TAATTCACTC CCAACGAAGA TAAGATCTGC TTTTTGCTTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGTAGT AGTTCATGTC ATCTTATTAT TCAGTATTTA TAACTTGCAA AGAAATGAAT ATCAGAGAGT GAGAGGAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGGCT CTAGCTATCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGACTT TTGCAGAGAC CAAATTCGTA ATCATGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGCCAAGCT G
