NM_000088.4;CAFYD; EDSARTH1; EDSC; OI1; OI2; OI3; OI4
100μg liquid plasmid(Instantly):$134
| name | pCMV-COL1A1(human)-3×FLAG-Neo | another name | apCMV-COL1A1(human)-3×FLAG-Neo |
| Insert Size (bp) | 4392bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCATGTT CAGCTTTGTG GACCTCCGGC TCCTGCTCCT CTTAGCGGCC ACCGCCCTCC TGACGCACGG CCAAGAGGAA GGCCAAGTCG AGGGCCAAGA CGAAGACATC CCACCAATCA CCTGCGTACA GAACGGCCTC AGGTACCATG ACCGAGACGT GTGGAAACCC GAGCCCTGCC GGATCTGCGT CTGCGACAAC GGCAAGGTGT TGTGCGATGA CGTGATCTGT GACGAGACCA AGAACTGCCC CGGCGCCGAA GTCCCCGAGG GCGAGTGCTG TCCCGTCTGC CCCGACGGCT CAGAGTCACC CACCGACCAA GAAACCACCG GCGTCGAGGG ACCCAAGGGA GACACTGGCC CCCGAGGCCC AAGGGGACCC GCAGGCCCCC CTGGCCGAGA TGGCATCCCT GGACAGCCTG GACTTCCCGG ACCCCCCGGA CCCCCCGGAC CTCCCGGACC CCCTGGCCTC GGAGGAAACT TTGCTCCCCA GCTGTCTTAT GGCTATGATG AGAAATCAAC CGGAGGAATT TCCGTGCCTG GCCCCATGGG TCCCTCTGGT CCTCGTGGTC TCCCTGGCCC CCCTGGTGCA CCTGGTCCCC AAGGCTTCCA AGGTCCCCCT GGTGAGCCTG GCGAGCCTGG AGCTTCAGGT CCCATGGGTC CCCGAGGTCC CCCAGGTCCC CCTGGAAAGA ATGGAGATGA TGGGGAAGCT GGAAAACCTG GTCGTCCTGG TGAGCGTGGG CCTCCTGGGC CTCAGGGTGC TCGAGGATTG CCCGGAACAG CTGGCCTCCC TGGAATGAAG GGACACAGAG GTTTCAGTGG TTTGGATGGT GCCAAGGGAG ATGCTGGTCC TGCTGGTCCT AAGGGTGAGC CTGGCAGCCC TGGTGAAAAT GGAGCTCCTG GTCAGATGGG CCCCCGTGGC CTGCCTGGTG AGAGAGGTCG CCCTGGAGCC CCTGGCCCTG CTGGTGCTCG TGGAAATGAT GGTGCTACTG GTGCTGCCGG GCCCCCTGGT CCCACCGGCC CCGCTGGTCC TCCTGGCTTC CCTGGTGCTG TTGGTGCTAA GGGTGAAGCT GGTCCCCAAG GGCCCCGAGG CTCTGAAGGT CCCCAGGGTG TGCGTGGTGA GCCTGGCCCC CCTGGCCCTG CTGGTGCTGC TGGCCCTGCT GGAAACCCTG GTGCTGATGG ACAGCCTGGT GCTAAAGGTG CCAATGGTGC TCCTGGTATT GCTGGTGCTC CTGGCTTCCC TGGTGCCCGA GGCCCCTCTG GACCCCAGGG CCCCGGCGGC CCTCCTGGTC CCAAGGGTAA CAGCGGTGAA CCTGGTGCTC CTGGCAGCAA AGGAGACACT GGTGCTAAGG GAGAGCCTGG CCCTGTTGGT GTTCAAGGAC CCCCTGGCCC TGCTGGAGAG GAAGGAAAGC GAGGAGCTCG AGGTGAACCC GGACCCACTG GCCTGCCCGG ACCCCCTGGC GAGCGTGGTG GACCTGGTAG CCGTGGTTTC CCTGGCGCAG ATGGTGTTGC TGGTCCCAAG GGTCCCGCTG GTGAACGTGG TTCTCCTGGC CCTGCTGGCC CCAAAGGATC TCCTGGTGAA GCTGGTCGTC CCGGTGAAGC TGGTCTGCCT GGTGCCAAGG GTCTGACTGG AAGCCCTGGC AGCCCTGGTC CTGATGGCAA AACTGGCCCC CCTGGTCCCG CCGGTCAAGA TGGTCGCCCC GGACCCCCAG GCCCACCTGG TGCCCGTGGT CAGGCTGGTG TGATGGGATT CCCTGGACCT AAAGGTGCTG CTGGAGAGCC CGGCAAGGCT GGAGAGCGAG GTGTTCCCGG ACCCCCTGGC GCTGTCGGTC CTGCTGGCAA AGATGGAGAG GCTGGAGCTC AGGGACCCCC TGGCCCTGCT GGTCCCGCTG GCGAGAGAGG TGAACAAGGC CCTGCTGGCT CCCCCGGATT CCAGGGTCTC CCTGGTCCTG CTGGTCCTCC AGGTGAAGCA GGCAAACCTG GTGAACAGGG TGTTCCTGGA GACCTTGGCG CCCCTGGCCC CTCTGGAGCA AGAGGCGAGA GAGGTTTCCC TGGCGAGCGT GGTGTGCAAG GTCCCCCTGG TCCTGCTGGT CCCCGAGGGG CCAACGGTGC TCCCGGCAAC GATGGTGCTA AGGGTGATGC TGGTGCCCCT GGAGCTCCCG GTAGCCAGGG CGCCCCTGGC CTTCAGGGAA TGCCTGGTGA ACGTGGTGCA GCTGGTCTTC CAGGGCCTAA GGGTGACAGA GGTGATGCTG GTCCCAAAGG TGCTGATGGC TCTCCTGGCA AAGATGGCGT CCGTGGTCTG ACTGGCCCCA TTGGTCCTCC TGGCCCTGCT GGTGCCCCTG GTGACAAGGG TGAAAGTGGT CCCAGCGGCC CTGCTGGTCC CACTGGAGCT CGTGGTGCCC CCGGAGACCG TGGTGAGCCT GGTCCCCCCG GCCCTGCTGG CTTTGCTGGC CCCCCTGGTG CTGACGGCCA ACCTGGTGCT AAAGGCGAAC CTGGTGATGC TGGTGCTAAA GGCGATGCTG GTCCCCCTGG CCCTGCCGGA CCCGCTGGAC CCCCTGGCCC CATTGGTAAT GTTGGTGCTC CTGGAGCCAA AGGTGCTCGC GGCAGCGCTG GTCCCCCTGG TGCTACTGGT TTCCCTGGTG CTGCTGGCCG AGTCGGTCCT CCTGGCCCCT CTGGAAATGC TGGACCCCCT GGCCCTCCTG GTCCTGCTGG CAAAGAAGGC GGCAAAGGTC CCCGTGGTGA GACTGGCCCT GCTGGACGTC CTGGTGAAGT TGGTCCCCCT GGTCCCCCTG GCCCTGCTGG CGAGAAAGGA TCCCCTGGTG CTGATGGTCC TGCTGGTGCT CCTGGTACTC CCGGGCCTCA AGGTATTGCT GGACAGCGTG GTGTGGTCGG CCTGCCTGGT CAGAGAGGAG AGAGAGGCTT CCCTGGTCTT CCTGGCCCCT CTGGTGAACC TGGCAAACAA GGTCCCTCTG GAGCAAGTGG TGAACGTGGT CCCCCTGGTC CCATGGGCCC CCCTGGATTG GCTGGACCCC CTGGTGAATC TGGACGTGAG GGGGCTCCTG GTGCCGAAGG TTCCCCTGGA CGAGACGGTT CTCCTGGCGC CAAGGGTGAC CGTGGTGAGA CCGGCCCCGC TGGACCCCCT GGTGCTCCTG GTGCTCCTGG TGCCCCTGGC CCCGTTGGCC CTGCTGGCAA GAGTGGTGAT CGTGGTGAGA CTGGTCCTGC TGGTCCCGCC GGTCCTGTCG GCCCTGTTGG CGCCCGTGGC CCCGCCGGAC CCCAAGGCCC CCGTGGTGAC AAGGGTGAGA CAGGCGAACA GGGCGACAGA GGCATAAAGG GTCACCGTGG CTTCTCTGGC CTCCAGGGTC CCCCTGGCCC TCCTGGCTCT CCTGGTGAAC AAGGTCCCTC TGGAGCCTCT GGTCCTGCTG GTCCCCGAGG TCCCCCTGGC TCTGCTGGTG CTCCTGGCAA AGATGGACTC AACGGTCTCC CTGGCCCCAT TGGGCCCCCT GGTCCTCGCG GTCGCACTGG TGATGCTGGT CCTGTTGGTC CCCCCGGCCC TCCTGGACCT CCTGGTCCCC CTGGTCCTCC CAGCGCTGGT TTCGACTTCA GCTTCCTGCC CCAGCCACCT CAAGAGAAGG CTCACGATGG TGGCCGCTAC TACCGGGCTG ATGATGCCAA TGTGGTTCGT GACCGTGACC TCGAGGTGGA CACCACCCTC AAGAGCCTGA GCCAGCAGAT CGAGAACATC CGGAGCCCAG AGGGCAGCCG CAAGAACCCC GCCCGCACCT GCCGTGACCT CAAGATGTGC CACTCTGACT GGAAGAGTGG AGAGTACTGG ATTGACCCCA ACCAAGGCTG CAACCTGGAT GCCATCAAAG TCTTCTGCAA CATGGAGACT GGTGAGACCT GCGTGTACCC CACTCAGCCC AGTGTGGCCC AGAAGAACTG GTACATCAGC AAGAACCCCA AGGACAAGAG GCATGTCTGG TTCGGCGAGA GCATGACCGA TGGATTCCAG TTCGAGTATG GCGGCCAGGG CTCCGACCCT GCCGATGTGG CCATCCAGCT GACCTTCCTG CGCCTGATGT CCACCGAGGC CTCCCAGAAC ATCACCTACC ACTGCAAGAA CAGCGTGGCC TACATGGACC AGCAGACTGG CAACCTCAAG AAGGCCCTGC TCCTCCAGGG CTCCAACGAG ATCGAGATCC GCGCCGAGGG CAACAGCCGC TTCACCTACA GCGTCACTGT CGATGGCTGC ACGAGTCACA CCGGAGCCTG GGGCAAGACA GTGATTGAAT ACAAAACCAC CAAGACCTCC CGCCTGCCCA TCATCGATGT GGCCCCCTTG GACGTTGGTG CCCCAGACCA GGAATTCGGC TTCGACGTTG GCCCTGTCTG CTTCCTGGGA TCCGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG ACATCGACTA CAAGGATGAC GATGACAAGT AGTGAGGGCC CGATATCTCG ACAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAGCT GACGTCCTTT CCATGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG CCTGGAAACG GGGGAGGCTA ACTGAAACAC GGAAGGAGAC AATACCGGAA GGAACCCGCG CTATGACGGC AATAAAAAGA CAGAATAAAA CGCACGGGTG TTGGGTCGTT TGTTCATAAA CGCGGGGTTC GGTCCCAGGG CTGGCACTCT GTCGATACCC CACCGAGACC CCATTGGGGC CAATACGCCC GCGTTTCTTC CTTTTCCCCA CCCCACCCCC CAAGTTCGGG TGAAGGCCCA GGGCTCGCAG CCAACGTCGG GGCGGCAGGC CCTGCCATAG CAGATCTGCG CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGCATCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGGGG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC AATGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCGACGGATC GGGAGATCTC CCGATCCCCT ATGGTCGACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATCTGCTCCC TGCTTGTGTG TTGGAGGTCG CTGAGTAGTG CGCGAGCAAA ATTTAAGCTA CAACAAGGCA AGGCTTGACC GACAATTGCA TGAAGAATCT GCTTAGG
