NM_000088.4;CAFYD; EDSARTH1; EDSC; OI1; OI2; OI3; OI4
100μg liquid plasmid(Instantly):$134
| name | pLV3-CMV-COL1A1(human)-Zeo | another name | apLV3-CMV-COL1A1(human)-Zeo |
| Insert Size (bp) | 4395bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Zeo |
| Selective marker | Zeo | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCGCG CAATTAACCC TCACTAAAGG GAACAAAAGC TGGAGCTGCA AGCTTAATGT AGTCTTATGC AATACTCTTG TAGTCTTGCA ACATGGTAAC GATGAGTTAG CAACATGCCT TACAAGGAGA GAAAAAGCAC CGTGCATGCC GATTGGTGGA AGTAAGGTGG TACGATCGTG CCTTATTAGG AAGGCAACAG ACGGGTCTGA CATGGATTGG ACGAACCACT GAATTGCCGC ATTGCAGAGA TATTGTATTT AAGTGCCTAG CTCGATACAT AAACGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG GGACTTGAAA GCGAAAGGGA AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG GCAAGAGGCG AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAGGCTAG AAGGAGAGAG ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGAGAATTAG ATCGCGATGG GAAAAAATTC GGTTAAGGCC AGGGGGAAAG AAAAAATATA AATTAAAACA TATAGTATGG GCAAGCAGGG AGCTAGAACG ATTCGCAGTT AATCCTGGCC TGTTAGAAAC ATCAGAAGGC TGTAGACAAA TACTGGGACA GCTACAACCA TCCCTTCAGA CAGGATCAGA AGAACTTAGA TCATTATATA ATACAGTAGC AACCCTCTAT TGTGTGCATC AAAGGATAGA GATAAAAGAC ACCAAGGAAG CTTTAGACAA GATAGAGGAA GAGCAAAACA AAAGTAAGAC CACCGCACAG CAAGCGGCCG CTGATCTTCA GACCTGGAGG AGGAGATATG AGGGACAATT GGAGAAGTGA ATTATATAAA TATAAAGTAG TAAAAATTGA ACCATTAGGA GTAGCACCCA CCAAGGCAAA GAGAAGAGTG GTGCAGAGAG AAAAAAGAGC AGTGGGAATA GGAGCTTTGT TCCTTGGGTT CTTGGGAGCA GCAGGAAGCA CTATGGGCGC AGCGTCAATG ACGCTGACGG TACAGGCCAG ACAATTATTG TCTGGTATAG TGCAGCAGCA GAACAATTTG CTGAGGGCTA TTGAGGCGCA ACAGCATCTG TTGCAACTCA CAGTCTGGGG CATCAAGCAG CTCCAGGCAA GAATCCTGGC TGTGGAAAGA TACCTAAAGG ATCAACAGCT CCTGGGGATT TGGGGTTGCT CTGGAAAACT CATTTGCACC ACTGCTGTGC CTTGGAATGC TAGTTGGAGT AATAAATCTC TGGAACAGAT TTGGAATCAC ACGACCTGGA TGGAGTGGGA CAGAGAAATT AACAATTACA CAAGCTTAAT ACACTCCTTA ATTGAAGAAT CGCAAAACCA GCAAGAAAAG AATGAACAAG AATTATTGGA ATTAGATAAA TGGGCAAGTT TGTGGAATTG GTTTAACATA ACAAATTGGC TGTGGTATAT AAAATTATTC ATAATGATAG TAGGAGGCTT GGTAGGTTTA AGAATAGTTT TTGCTGTACT TTCTATAGTG AATAGAGTTA GGCAGGGATA TTCACCATTA TCGTTTCAGA CCCACCTCCC AACCCCGAGG GGACCCGACA GGCCCGAAGG AATAGAAGAA GAAGGTGGAG AGAGAGACAG AGACAGATCC ATTCGATTAG TGAACGGATC TCGACGGTAT CGGTTAACTT TTAAAAGAAA AGGGGGGATT GGGGGGTACA GTGCAGGGGA AAGAATAGTA GACATAATAG CAACAGACAT ACAAACTAAA GAATTACAAA AACAAATTAC AAAATTCAAA ATTTTATCGA TAAGCTTGGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT TTTGACCTCC ATAGAAGACA CCGACTCTAG AGGATCCGCC ACCATGTTCA GCTTTGTGGA CCTCCGGCTC CTGCTCCTCT TAGCGGCCAC CGCCCTCCTG ACGCACGGCC AAGAGGAAGG CCAAGTCGAG GGCCAAGACG AAGACATCCC ACCAATCACC TGCGTACAGA ACGGCCTCAG GTACCATGAC CGAGACGTGT GGAAACCCGA GCCCTGCCGG ATCTGCGTCT GCGACAACGG CAAGGTGTTG TGCGATGACG TGATCTGTGA CGAGACCAAG AACTGCCCCG GCGCCGAAGT CCCCGAGGGC GAGTGCTGTC CCGTCTGCCC CGACGGCTCA GAGTCACCCA CCGACCAAGA AACCACCGGC GTCGAGGGAC CCAAGGGAGA CACTGGCCCC CGAGGCCCAA GGGGACCCGC AGGCCCCCCT GGCCGAGATG GCATCCCTGG ACAGCCTGGA CTTCCCGGAC CCCCCGGACC CCCCGGACCT CCCGGACCCC CTGGCCTCGG AGGAAACTTT GCTCCCCAGC TGTCTTATGG CTATGATGAG AAATCAACCG GAGGAATTTC CGTGCCTGGC CCCATGGGTC CCTCTGGTCC TCGTGGTCTC CCTGGCCCCC CTGGTGCACC TGGTCCCCAA GGCTTCCAAG GTCCCCCTGG TGAGCCTGGC GAGCCTGGAG CTTCAGGTCC CATGGGTCCC CGAGGTCCCC CAGGTCCCCC TGGAAAGAAT GGAGATGATG GGGAAGCTGG AAAACCTGGT CGTCCTGGTG AGCGTGGGCC TCCTGGGCCT CAGGGTGCTC GAGGATTGCC CGGAACAGCT GGCCTCCCTG GAATGAAGGG ACACAGAGGT TTCAGTGGTT TGGATGGTGC CAAGGGAGAT GCTGGTCCTG CTGGTCCTAA GGGTGAGCCT GGCAGCCCTG GTGAAAATGG AGCTCCTGGT CAGATGGGCC CCCGTGGCCT GCCTGGTGAG AGAGGTCGCC CTGGAGCCCC TGGCCCTGCT GGTGCTCGTG GAAATGATGG TGCTACTGGT GCTGCCGGGC CCCCTGGTCC CACCGGCCCC GCTGGTCCTC CTGGCTTCCC TGGTGCTGTT GGTGCTAAGG GTGAAGCTGG TCCCCAAGGG CCCCGAGGCT CTGAAGGTCC CCAGGGTGTG CGTGGTGAGC CTGGCCCCCC TGGCCCTGCT GGTGCTGCTG GCCCTGCTGG AAACCCTGGT GCTGATGGAC AGCCTGGTGC TAAAGGTGCC AATGGTGCTC CTGGTATTGC TGGTGCTCCT GGCTTCCCTG GTGCCCGAGG CCCCTCTGGA CCCCAGGGCC CCGGCGGCCC TCCTGGTCCC AAGGGTAACA GCGGTGAACC TGGTGCTCCT GGCAGCAAAG GAGACACTGG TGCTAAGGGA GAGCCTGGCC CTGTTGGTGT TCAAGGACCC CCTGGCCCTG CTGGAGAGGA AGGAAAGCGA GGAGCTCGAG GTGAACCCGG ACCCACTGGC CTGCCCGGAC CCCCTGGCGA GCGTGGTGGA CCTGGTAGCC GTGGTTTCCC TGGCGCAGAT GGTGTTGCTG GTCCCAAGGG TCCCGCTGGT GAACGTGGTT CTCCTGGCCC TGCTGGCCCC AAAGGATCTC CTGGTGAAGC TGGTCGTCCC GGTGAAGCTG GTCTGCCTGG TGCCAAGGGT CTGACTGGAA GCCCTGGCAG CCCTGGTCCT GATGGCAAAA CTGGCCCCCC TGGTCCCGCC GGTCAAGATG GTCGCCCCGG ACCCCCAGGC CCACCTGGTG CCCGTGGTCA GGCTGGTGTG ATGGGATTCC CTGGACCTAA AGGTGCTGCT GGAGAGCCCG GCAAGGCTGG AGAGCGAGGT GTTCCCGGAC CCCCTGGCGC TGTCGGTCCT GCTGGCAAAG ATGGAGAGGC TGGAGCTCAG GGACCCCCTG GCCCTGCTGG TCCCGCTGGC GAGAGAGGTG AACAAGGCCC TGCTGGCTCC CCCGGATTCC AGGGTCTCCC TGGTCCTGCT GGTCCTCCAG GTGAAGCAGG CAAACCTGGT GAACAGGGTG TTCCTGGAGA CCTTGGCGCC CCTGGCCCCT CTGGAGCAAG AGGCGAGAGA GGTTTCCCTG GCGAGCGTGG TGTGCAAGGT CCCCCTGGTC CTGCTGGTCC CCGAGGGGCC AACGGTGCTC CCGGCAACGA TGGTGCTAAG GGTGATGCTG GTGCCCCTGG AGCTCCCGGT AGCCAGGGCG CCCCTGGCCT TCAGGGAATG CCTGGTGAAC GTGGTGCAGC TGGTCTTCCA GGGCCTAAGG GTGACAGAGG TGATGCTGGT CCCAAAGGTG CTGATGGCTC TCCTGGCAAA GATGGCGTCC GTGGTCTGAC TGGCCCCATT GGTCCTCCTG GCCCTGCTGG TGCCCCTGGT GACAAGGGTG AAAGTGGTCC CAGCGGCCCT GCTGGTCCCA CTGGAGCTCG TGGTGCCCCC GGAGACCGTG GTGAGCCTGG TCCCCCCGGC CCTGCTGGCT TTGCTGGCCC CCCTGGTGCT GACGGCCAAC CTGGTGCTAA AGGCGAACCT GGTGATGCTG GTGCTAAAGG CGATGCTGGT CCCCCTGGCC CTGCCGGACC CGCTGGACCC CCTGGCCCCA TTGGTAATGT TGGTGCTCCT GGAGCCAAAG GTGCTCGCGG CAGCGCTGGT CCCCCTGGTG CTACTGGTTT CCCTGGTGCT GCTGGCCGAG TCGGTCCTCC TGGCCCCTCT GGAAATGCTG GACCCCCTGG CCCTCCTGGT CCTGCTGGCA AAGAAGGCGG CAAAGGTCCC CGTGGTGAGA CTGGCCCTGC TGGACGTCCT GGTGAAGTTG GTCCCCCTGG TCCCCCTGGC CCTGCTGGCG AGAAAGGATC CCCTGGTGCT GATGGTCCTG CTGGTGCTCC TGGTACTCCC GGGCCTCAAG GTATTGCTGG ACAGCGTGGT GTGGTCGGCC TGCCTGGTCA GAGAGGAGAG AGAGGCTTCC CTGGTCTTCC TGGCCCCTCT GGTGAACCTG GCAAACAAGG TCCCTCTGGA GCAAGTGGTG AACGTGGTCC CCCTGGTCCC ATGGGCCCCC CTGGATTGGC TGGACCCCCT GGTGAATCTG GACGTGAGGG GGCTCCTGGT GCCGAAGGTT CCCCTGGACG AGACGGTTCT CCTGGCGCCA AGGGTGACCG TGGTGAGACC GGCCCCGCTG GACCCCCTGG TGCTCCTGGT GCTCCTGGTG CCCCTGGCCC CGTTGGCCCT GCTGGCAAGA GTGGTGATCG TGGTGAGACT GGTCCTGCTG GTCCCGCCGG TCCTGTCGGC CCTGTTGGCG CCCGTGGCCC CGCCGGACCC CAAGGCCCCC GTGGTGACAA GGGTGAGACA GGCGAACAGG GCGACAGAGG CATAAAGGGT CACCGTGGCT TCTCTGGCCT CCAGGGTCCC CCTGGCCCTC CTGGCTCTCC TGGTGAACAA GGTCCCTCTG GAGCCTCTGG TCCTGCTGGT CCCCGAGGTC CCCCTGGCTC TGCTGGTGCT CCTGGCAAAG ATGGACTCAA CGGTCTCCCT GGCCCCATTG GGCCCCCTGG TCCTCGCGGT CGCACTGGTG ATGCTGGTCC TGTTGGTCCC CCCGGCCCTC CTGGACCTCC TGGTCCCCCT GGTCCTCCCA GCGCTGGTTT CGACTTCAGC TTCCTGCCCC AGCCACCTCA AGAGAAGGCT CACGATGGTG GCCGCTACTA CCGGGCTGAT GATGCCAATG TGGTTCGTGA CCGTGACCTC GAGGTGGACA CCACCCTCAA GAGCCTGAGC CAGCAGATCG AGAACATCCG GAGCCCAGAG GGCAGCCGCA AGAACCCCGC CCGCACCTGC CGTGACCTCA AGATGTGCCA CTCTGACTGG AAGAGTGGAG AGTACTGGAT TGACCCCAAC CAAGGCTGCA ACCTGGATGC CATCAAAGTC TTCTGCAACA TGGAGACTGG TGAGACCTGC GTGTACCCCA CTCAGCCCAG TGTGGCCCAG AAGAACTGGT ACATCAGCAA GAACCCCAAG GACAAGAGGC ATGTCTGGTT CGGCGAGAGC ATGACCGATG GATTCCAGTT CGAGTATGGC GGCCAGGGCT CCGACCCTGC CGATGTGGCC ATCCAGCTGA CCTTCCTGCG CCTGATGTCC ACCGAGGCCT CCCAGAACAT CACCTACCAC TGCAAGAACA GCGTGGCCTA CATGGACCAG CAGACTGGCA ACCTCAAGAA GGCCCTGCTC CTCCAGGGCT CCAACGAGAT CGAGATCCGC GCCGAGGGCA ACAGCCGCTT CACCTACAGC GTCACTGTCG ATGGCTGCAC GAGTCACACC GGAGCCTGGG GCAAGACAGT GATTGAATAC AAAACCACCA AGACCTCCCG CCTGCCCATC ATCGATGTGG CCCCCTTGGA CGTTGGTGCC CCAGACCAGG AATTCGGCTT CGACGTTGGC CCTGTCTGCT TCCTGTAAGT CGACAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAG CTGACGTCCT TTCCATGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCCTGGAAT TCTGCAGATA TCCGGTTAGT AATGAGTTTG GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCCCTG TTGACAATTA ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGGC CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG GACGACTTCG CCGGTGTGGT CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC CAGGTGGTGC CGGACAACAC CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC GAGATCGGCG AGCAGCCGTG GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC GTGCACTTCG TGGCCGAGGA GCAGGACTGA CACGTGCTAC GAGATTTAAA TGGTACCTTT AAGACCAATG ACTTACAAGG CAGCTGTAGA TCTTAGCCAC TTTTTAAAAG AAAAGGGGGG ACTGGAAGGG CTAGCTCACT CCCAACGAAG ACAAGATCTG CTTTTTGCTT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT TTAGTCAGTG TGGAAAATCT CTAGCAGTAG TAGTTCATGT CATCTTATTA TTCAGTATTT ATAACTTGCA AAGAAATGAA TATCAGAGAG TGAGAGGAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGC TCTAGCTATC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCG
