pBECKP-Apr
$99.00
In stock
SKU
PL37084
100μg liquid plasmid(Instantly):$142
| name | pBECKP-Apr | another name | apBECKP-Apr |
| Insert Size (bp) | Plasmid host | Klebsiella pneumoniae | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Empty vector | Prokaryotic resistance | Apr |
| Selective marker | Fluorescent labeling | ||
| Promoter | J23119 | Replicon | pUC-rop |
| Competence | DH5a | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | Low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ATGAGTAGCG AAACCGGTCC GGTTGCAGTG GATCCGACCC TGCGCCGTCG CATTGAACCG CACGAGTTTG AAGTGTTCTT TGATCCGCGC GAGCTGCGCA AAGAAACTTG CCTGCTGTAC GAGATTAACT GGGGTGGCCG CCATAGCATC TGGCGCCATA CCAGCCAGAA CACCAATAAG CACGTGGAAG TGAATTTTAT TGAAAAATTT ACCACCGAGC GCTACTTCTG CCCTAATACC CGCTGCAGCA TCACCTGGTT TCTGAGCTGG AGCCCGTGCG GCGAATGTAG TCGCGCCATT ACCGAGTTCC TGAGCCGCTA TCCGCATGTG ACCCTGTTCA TCTACATCGC CCGTCTGTAC CATCACGCCG ATCCGCGCAA TCGCCAAGGT CTGCGTGATC TGATTAGCAG CGGTGTGACC ATCCAGATCA TGACCGAACA AGAGAGCGGC TATTGCTGGC GCAACTTCGT GAACTATTCT CCGAGCAACG AAGCCCACTG GCCGCGTTAT CCGCATCTGT GGGTGCGCCT GTATGTGCTG GAGCTGTACT GCATCATCCT GGGCCTGCCG CCTTGCCTGA ATATTCTGCG CCGTAAACAG CCGCAACTGA CATTCTTCAC CATCGCACTG CAGAGCTGCC ATTATCAGCG CCTGCCGCCG CACATTTTAT GGGCCACCGG TCTGAAAAGC GGTAGTGAAA CTCCGGGCAC AAGCGAAAGC GCAACCCCGG AAAGTGATAA GAAATACTCA ATAGGCTTAG CTATCGGCAC AAATAGCGTC GGATGGGCGG TGATCACTGA TGAATATAAG GTTCCGTCTA AAAAGTTCAA GGTTCTGGGA AATACAGACC GCCACAGTAT CAAAAAAAAT CTTATAGGGG CTCTTTTATT TGACAGTGGA GAGACAGCGG AAGCGACTCG TCTCAAACGG ACAGCTCGTA GAAGGTATAC ACGTCGGAAG AATCGTATTT GTTATCTACA GGAGATTTTT TCAAATGAGA TGGCGAAAGT AGATGATAGT TTCTTTCATC GACTTGAAGA GTCTTTTTTG GTGGAAGAAG ACAAGAAGCA TGAACGTCAT CCTATTTTTG GAAATATAGT AGATGAAGTT GCTTATCATG AGAAATATCC AACTATCTAT CATCTGCGAA AAAAATTGGT AGATTCTACT GATAAAGCGG ATTTGCGCTT AATCTATTTG GCCTTAGCGC ATATGATTAA GTTTCGTGGT CATTTTTTGA TTGAGGGAGA TTTAAATCCT GATAATAGTG ATGTGGACAA ACTATTTATC CAGTTGGTAC AAACCTACAA TCAATTATTT GAAGAAAACC CTATTAACGC AAGTGGAGTA GATGCTAAAG CGATTCTTTC TGCACGATTG AGTAAATCAA GACGATTAGA AAATCTCATT GCTCAGCTCC CCGGTGAGAA GAAAAATGGC TTATTTGGGA ATCTCATTGC TTTGTCATTG GGTTTGACCC CTAATTTTAA ATCAAATTTT GATTTGGCAG AAGATGCTAA ATTACAGCTT TCAAAAGATA CTTACGATGA TGATTTAGAT AATTTATTGG CGCAAATTGG AGATCAATAT GCTGATTTGT TTTTGGCAGC TAAGAATTTA TCAGATGCTA TTTTACTTTC AGATATCCTA AGAGTAAATA CTGAAATAAC TAAGGCTCCC CTATCAGCTT CAATGATTAA ACGCTACGAT GAACATCATC AAGACTTGAC TCTTTTAAAA GCTTTAGTTC GACAACAACT TCCAGAAAAG TATAAAGAAA TCTTTTTTGA TCAATCAAAA AACGGATATG CAGGTTATAT TGATGGGGGA GCTAGCCAAG AAGAATTTTA TAAATTTATC AAACCAATTT TAGAAAAAAT GGATGGTACT GAGGAATTAT TGGTGAAACT AAATCGTGAA GATTTGCTGC GCAAGCAACG GACCTTTGAC AACGGCTCTA TTCCCCATCA AATTCACTTG GGTGAGCTGC ATGCTATTTT GAGAAGACAA GAAGACTTTT ATCCATTTTT AAAAGACAAT CGTGAGAAGA TTGAAAAAAT CTTGACTTTT CGAATTCCTT ATTATGTTGG TCCATTGGCG CGTGGCAATA GTCGTTTTGC ATGGATGACT CGGAAGTCTG AAGAAACAAT TACCCCATGG AATTTTGAAG AAGTTGTCGA TAAAGGTGCT TCAGCTCAAT CATTTATTGA ACGCATGACA AACTTTGATA AAAATCTTCC AAATGAAAAA GTACTACCAA AACATAGTTT GCTTTATGAG TATTTTACGG TTTATAACGA ATTGACAAAG GTCAAATATG TTACTGAAGG AATGCGAAAA CCAGCATTTC TTTCAGGTGA ACAGAAGAAA GCCATTGTTG ATTTACTCTT CAAAACAAAT CGAAAAGTAA CCGTTAAGCA ATTAAAAGAA GATTATTTCA AAAAAATAGA ATGTTTTGAT AGTGTTGAAA TTTCAGGAGT TGAAGATAGA TTTAATGCTT CATTAGGTAC CTACCATGAT TTGCTAAAAA TTATTAAAGA TAAAGATTTT TTGGATAATG AAGAAAATGA AGATATCTTA GAGGATATTG TTTTAACATT GACCTTATTT GAAGATAGGG AGATGATTGA GGAAAGACTT AAAACATATG CTCACCTCTT TGATGATAAG GTGATGAAAC AGCTTAAACG TCGCCGTTAT ACTGGTTGGG GACGTTTGTC TCGAAAATTG ATTAATGGTA TTAGGGATAA GCAATCTGGC AAAACAATAT TAGATTTTTT GAAATCAGAT GGTTTTGCCA ATCGCAATTT TATGCAGCTG ATCCATGATG ATAGTTTGAC ATTTAAAGAA GACATTCAAA AAGCACAAGT GTCTGGACAA GGCGATAGTT TACATGAACA TATTGCAAAT TTAGCTGGTA GCCCTGCTAT TAAAAAAGGT ATTTTACAGA CTGTAAAAGT TGTTGATGAA TTGGTCAAAG TAATGGGGCG GCATAAGCCA GAAAATATCG TTATTGAAAT GGCACGTGAA AATCAGACAA CTCAAAAGGG CCAGAAAAAT TCGCGAGAGC GTATGAAACG AATCGAAGAA GGTATCAAAG AATTAGGAAG TCAGATTCTT AAAGAGCATC CTGTTGAAAA TACTCAATTG CAAAATGAAA AGCTCTATCT CTATTATCTC CAAAATGGAA GAGACATGTA TGTGGACCAA GAATTAGATA TTAATCGTTT AAGTGATTAT GATGTCGATC ACATTGTTCC ACAAAGTTTC CTTAAAGACG ATTCAATAGA CAATAAGGTC TTAACGCGTT CTGATAAAAA TCGTGGTAAA TCGGATAACG TTCCAAGTGA AGAAGTAGTC AAAAAGATGA AAAACTATTG GAGACAACTT CTAAACGCCA AGTTAATCAC TCAACGTAAG TTTGATAATT TAACGAAAGC TGAACGTGGA GGTTTGAGTG AACTTGATAA AGCTGGTTTT ATCAAACGCC AATTGGTTGA AACTCGCCAA ATCACTAAGC ATGTGGCACA AATTTTGGAT AGTCGCATGA ATACTAAATA CGATGAAAAT GATAAACTTA TTCGAGAGGT TAAAGTGATT ACCTTAAAAT CTAAATTAGT TTCTGACTTC CGAAAAGATT TCCAATTCTA TAAAGTACGT GAGATTAACA ATTACCATCA TGCCCATGAT GCGTATCTAA ATGCCGTCGT TGGAACTGCT TTGATTAAGA AATATCCAAA ACTTGAATCG GAGTTTGTCT ATGGTGATTA TAAAGTTTAT GATGTTCGTA AAATGATTGC TAAGTCTGAG CAAGAAATAG GCAAAGCAAC CGCAAAATAT TTCTTTTACT CTAATATCAT GAACTTCTTC AAAACAGAAA TTACACTTGC AAATGGAGAG ATTCGCAAAC GCCCTCTAAT CGAAACTAAT GGGGAAACTG GAGAAATTGT CTGGGATAAA GGGCGAGATT TTGCCACAGT GCGCAAAGTA TTGTCCATGC CCCAAGTCAA TATTGTCAAG AAAACAGAAG TACAGACAGG CGGATTCTCC AAGGAGTCAA TTTTACCAAA AAGAAATTCG GACAAGCTTA TTGCTCGTAA AAAAGACTGG GATCCAAAAA AATATGGTGG TTTTGATAGT CCAACGGTAG CTTATTCAGT CCTAGTGGTT GCTAAGGTGG AAAAAGGGAA ATCGAAGAAG TTAAAATCCG TTAAAGAGTT ACTAGGGATC ACAATTATGG AAAGAAGTTC CTTTGAAAAA AATCCGATTG ACTTTTTAGA AGCTAAAGGA TATAAGGAAG TTAAAAAAGA CTTAATCATT AAACTACCTA AATATAGTCT TTTTGAGTTA GAAAACGGTC GTAAACGGAT GCTGGCTAGT GCCGGAGAAT TACAAAAAGG AAATGAGCTG GCTCTGCCAA GCAAATATGT GAATTTTTTA TATTTAGCTA GTCATTATGA AAAGTTGAAG GGTAGTCCAG AAGATAACGA ACAAAAACAA TTGTTTGTGG AGCAGCATAA GCATTATTTA GATGAGATTA TTGAGCAAAT CAGTGAATTT TCTAAGCGTG TTATTTTAGC AGATGCCAAT TTAGATAAAG TTCTTAGTGC ATATAACAAA CATAGAGACA AACCAATACG TGAACAAGCA GAAAATATTA TTCATTTATT TACGTTGACG AATCTTGGAG CTCCCGCTGC TTTTAAATAT TTTGATACAA CAATTGATCG TAAACGATAT ACGTCTACAA AAGAAGTTTT AGATGCCACT CTTATCCATC AATCCATCAC TGGTCTTTAT GAAACACGCA TTGATTTGAG TCAGCTAGGA GGTGACTGAT GGCTGGTTGG CGTACTGTTG TGGTAAATAC CAAGCTTGAT ATCTTGACAG CTAGCTCAGT CCTAGGTATA ATACTAGTCG AGACCATTGG TCTCAGTTTT AGAGCTAGAA ATAGCAAGTT AAAATAAGGC TAGTCCGTTA TCAACTTGAA AAAGTGGCAC CGAGTCGGTG CTTTTTTTGA TATCGAATTC CTGCAGCCCG GGGGATCCAC TAGTTCTAGA GCGGCCGCCA CCGCGGTGGA GCTCATCCCA ATGGCGCGCC GAGCTTGGCT CGAGCATGGT CATAGCTGTT TCCTGCTCAC TCATTAGGCA CCGGGATCTC GACCGATGCC CTTGAGAGCC TTCAACCCAG TCAGCTCCTT CCGGTGGGCG CGGGGCATGA CTATCGTCGC CGCACTTATG ACTGTCTTCT TTATCATGCA ACTCGTAGGA CAGGTGCCGG CAGCGCTCTG GGTCATTTTC GGCGAGGACC GCTTTCGCTG GAGCGCGACG ATGATCGGCC TGTCGCTTGC GGTATTCGGA ATCTTGCACG CCCTCGCTCA AGCCTTCGTC ACTGGTCCCG CCACCAAACG TTTCGGCGAG AAGCAGGCCA TTATCGCCGG CATGGCGGCC CCACGGGTGC GCATGATCGT GCTCCTGTCG TTGAGGACCC GGCTAGGCTG GCGGGGTTGC CTTACTGGTT AGCAGAATGA ATCACCGATA CGCGAGCGAA CGTGAAGCGA CTGCTGCTGC AAAACGTCTG CGACCTGAGC AACAACATGA ATGGTCTTCG GTTTCCGTGT TTCGTAAAGT CTGGAAACGC GGAAGTCAGC GCCCTGCACC ATTATGTTCC GGATCTGCAT CGCAGGATGC TGCTGGCTAC CCTGTGGAAC ACCTACATCT GTATTAACGA AGCGCTGGCA TTGACCCTGA GTGATTTTTC TCTGGTCCCG CCGCATCCAT ACCGCCAGTT GTTTACCCTC ACAACGTTCC AGTAACCGGG CATGTTCATC ATCAGTAACC CGTATCGTGA GCATCCTCTC TCGTTTCATC GGTATCATTA CCCCCATGAA CAGAAATCCC CCTTACACGG AGGCATCAGT GACCAAACAG GAAAAAACCG CCCTTAACAT GGCCCGCTTT ATCAGAAGCC AGACATTAAC GCTTCTGGAG AAACTCAACG AGCTGGACGC GGATGAACAG GCAGACATCT GTGAATCGCT TCACGACCAC GCTGATGAGC TTTACCGCAG CTGCCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT GACCCAGTCA CGTAGCGATA GCGGAGTGTA TACTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TTGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GCAACTTTTG GCGAAAATGA GACGTTGATC GGCACGTAAG AGGTTCCAAC TTTCACCATA ATGAAATAAG ATCACTACCG GGCGTATTTT TTGAGTTATC GAGATTTTCA GGAGCTAAGG AAGCTAAAAT GGAGAAAAAA ATCACTGGAT ATACCACCGT TGATATATCC CAATGGCATC GTAAAGAACA TTTTGAGGCA TTTCAGTCAG TTGCTCAATG TACCTATAAC CAGACCGTTC AGCTGGGTCA TCCACCGGAT CAATTCCCCT GCTCGCGCAG GCTGGGTGCC AAGCTCTCGG GTAACATCAA GGCCCGATCC TTGGAGCCCT TGCCCTCCCG CACGATGATC GTGCCGTGAT CGAAATCCAG ATCCTTGACC CGCAGTTGCA AACCCTCACT GATCCGGCTC ACGGTAACTG ATGCCGTATT TGCAGTACCA GCGTACGGCC CACAGAATGA TGTCACGCTG AAAATGCCGG CCTTTGAATG GGTTCATGTG CAGCTCCATC AGCAAAAGGG GATGATAAGT TTATCACCAC CGACTATTTG CAACAGTGCC GTTGATCGTG CTATGATCGA CTGATGTCAT CAGCGGTGGA GTGCAATGTC GTGCAATACG AATGGCGAAA AGCCGAGCTC ATCGGTCAGC TTCTCAACCT TGGGGTTACC CCCGGCGGTG TGCTGCTGGT CCACAGCTCC TTCCGTAGCG TCCGGCCCCT CGAAGATGGG CCACTTGGAC TGATCGAGGC CCTGCGTGCT GCGCTGGGTC CGGGAGGGAC GCTCGTCATG CCCTCGTGGT CAGGTCTGGA CGACGAGCCG TTCGATCCTG CCACGTCGCC CGTTACACCG GACCTTGGAG TTGTCTCTGA CACATTCTGG CGCCTGCCAA ATGTAAAGCG CAGCGCCCAT CCATTTGCCT TTGCGGCAGC GGGGCCACAG GCAGAGCAGA TCATCTCTGA TCCATTGCCC CTGCCACCTC ACTCGCCTGC AAGCCCGGTC GCCCGTGTCC ATGAACTCGA TGGGCAGGTA CTTCTCCTCG GCGTGGGACA CGATGCCAAC ACGACGCTGC ATCTTGCCGA GTTGATGGCA AAGGTTCCCT ATGGGGTGCC GAGACACTGC ACCATTCTTC AGGATGGCAA GTTGGTACGC GTCGATTATC TCGAGAATGA CCACTGCTGT GAGCGCTTTG CCTTGGCGGA CAGGTGGCTC AAGGAGAAGA GCCTTCAGAA GGAAGGTCCA GTCGGTCATG CCTTTGCTCG GTTGATCCGC TCCCGCGACA TTGTGGCGAC AGCCCTGGGT CAACTGGGCC GAGATCCGTT GATCTTCCTG CATCCGCCAG AGGCGGGATG CGAAGAATGC GATGCCGCTC GCCAGTCGAT TGGCTGAGCT CATGAGCGGA GAACGAGATG ACGTCTCGAG GTCGACGGTA TCGATACGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TAATCGGCAT TTTCTTTTGC GTTTTTATTT GTTAACTGTT AATTGTCCTT GTTCAAGGAT GCTGTCTTTG ACAACAGATG TTTTCTTGCC TTTGATGTTC AGCAGGAAGC TAGGCGCAAA CGTTGATTGT TTGTCTGCGT AGAATCCTCT GTTTGTCATA TAGCTTGTAA TCACGACATT GTTTCCTTTC GCTTGAGGTA CAGCGAAGTG TGAGTAAGTA AAGGTTACAT CGTTAGGATC AAGATCCATT TTTAACACAA GGCCAGTTTT GTTCAGCGGC TTGTATGGGC CAGTTAAAGA ATTAGAAACA TAACCAAGCA TGTAAATATC GTTAGACGTA ATGCCGTCAA TCGTCATTTT TGATCCGCGG GAGTCAGTGA ACAGATACCA TTTGCCGTTC ATTTTAAAGA CGTTCGCGCG TTCAATTTCA TCTGTTACTG TGTTAGATGC AATCAGCGGT TTCATCACTT TTTTCAGTGT GTAATCATCG TTTAGCTCAA TCATACCGAG AGCGCCGTTT GCTAACTCAG CCGTGCGTTT TTTATCGCTT TGCAGAAGTT TTTGACTTTC TTGACGGAAG AATGATGTGC TTTTGCCATA GTATGCTTTG TTAAATAAAG ATTCTTCGCC TTGGTAGCCA TCTTCAGTTC CAGTGTTTGC TTCAAATACT AAGTATTTGT GGCCTTTATC TTCTACGTAG TGAGGATCTC TCAGCGTATG GTTGTCGCCT GAGCTGTAGT TGCCTTCATC GATGAACTGC TGTACATTTT GATACGTTTT TCCGTCACCG TCAAAGATTG ATTTATAATC CTCTACACCG TTGATGTTCA AAGAGCTGTC TGATGCTGAT ACGTTAACTT GTGCAGTTGT CAGTGTTTGT TTGCCGTAAT GTTTACCGGA GAAATCAGTG TAGAATAAAC GGATTTTTCC GTCAGATGTA AATGTGGCTG AACCTGACCA TTCTTGTGTT TGGTCTTTTA GGATAGAATC ATTTGCATCG AATTTGTCGC TGTCTTTAAA GACGCGGCCA GCGTTTTTCC AGCTGTCAAT AGAAGTTTCG CCGACTTTTT GATAGAACAT GTAAATCGAT GTGTCATCCG CATTTTTAGG ATCTCCGGCT AATGCAAAGA CGATGTGGTA GCCGTGATAG TTTGCGACAG TGCCGTCAGC GTTTTGTAAT GGCCAGCTGT CCCAAACGTC CAGGCCTTTT GCAGAAGAGA TATTTTTAAT TGTGGACGAA TCGAACTCAG GAACTTGATA TTTTTCATTT TTTTGCTGTT CAGGGATTTG CAGCATATCA TGGCGTGTAA TATGGGAAAT GCCGTATGTT TCCTTATATG GCTTTTGGTT CGTTTCTTTC GCAAACGCTT GAGTTGCGCC TCCTGCCAGC AGTGCGGTAG TAAAGGTTAA TACTGTTGCT TGTTTTGCAA ACTTTTTGAT GTTCATCGTT CATGTCTCCT TTTTTATGTA CTGTGTTAGC GGTCTGCTTC TTCCAGCCCT CCTGTTTGAA GATGGCAAGT TAGTTACGCA CAATAAAAAA AGACCTAAAA TATGTAAGGG GTGACGCCAA AGTATACACT TTGCCCTTTA CACATTTTAG GTCTTGCCTG CTTTATCAGT AACAAACCCG CGCGATTTAC TTTTCGACCT CATTCTATTA GACTCTCGTT TGGATTGCAA CTGGTCTATT TTCCTCTTTT GTTTGATAGA AAATCATAAA AGGATTTGCA GACTACGGGC CTAAAGAACT AAAAAATCTA TCTGTTTCTT TTCATTCTCT GTATTTTTTA TAGTTTCTGT TGCATGGGCA TAAAGTTGCA AGCTTGCAAA CATGCTTTGG GTAAACATGT AGGTATTAAC GTCAATGCGA CAATAGTAGC ATTGATTAAA TGAGAATTAG TAAGTGTTTT ACTTACTAAA TTTTATTTAA CCTAAAAATG AACCACCTGG ATGTGTGGGA TTAAAAAGTG AAGAGAGGAG GACATATCAC
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