pCMV-NLRP13(human)-3×FLAG-Neo
$99.00
In stock
SKU
PL37054
NM_176810.2;CLR19.7; NALP13; NOD14; PAN13
100μg liquid plasmid(Instantly):$120
| name | pCMV-NLRP13(human)-3×FLAG-Neo | another name | apCMV-NLRP13(human)-3×FLAG-Neo |
| Insert Size (bp) | 3129bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCATGAA CTTTTCTGTA ATCACCTGCC CCAACGGTGG TACCAACCAA GGGCTTCTGC CTTACCTGAT GGCCCTGGAT CAGTATCAGC TGGAGGAATT CAAGCTTTGC TTGGAACCCC AGCAGCTGAT GGACTTCTGG TCGGCCCCCC AGGGGCACTT CCCGCGTATC CCCTGGGCAA ACTTGAGAGC TGCCGACCCT TTGAATCTGT CCTTTCTTTT GGATGAACAC TTCCCAAAAG GTCAGGCATG GAAAGTGGTC CTCGGCATCT TCCAGACAAT GAATCTGACC TCACTGTGTG AGAAAGTTAG AGCCGAGATG AAAGAGAATG TGCAGACCCA AGAGCTGCAA GATCCAACCC AGGAAGATCT AGAGATGCTA GAAGCAGCAG CAGGGAATAT GCAGACCCAG GGATGCCAAG ATCCAAACCA AGAAGAACTA GACGAGCTAG AAGAAGAAAC AGGGAATGTA CAGGCCCAGG GATGCCAAGA TCCAAACCAA GAAGAACCAG AGATGCTAGA GGAAGCAGAC CACAGAAGAA AATACAGAGA GAACATGAAG GCTGAACTAC TGGAGACATG GGACAACATC AGTTGGCCTA AAGACCACGT ATATATCCGT AATACATCAA AGGACGAACA TGAGGAACTG CAGCGCCTAC TGGATCCTAA TAGGACTAGA GCCCAGGCCC AGACGATAGT CTTGGTGGGG AGGGCAGGGG TTGGGAAGAC CACCTTGGCA ATGCAGGCTA TGCTGCACTG GGCAAATGGA GTTCTCTTTC AGCAAAGGTT CTCCTATGTT TTCTATCTCA GCTGCCATAA AATAAGGTAC ATGAAGGAAA CTACCTTTGC TGAATTGATT TCTTTGGATT GGCCCGATTT TGATGCCCCC ATTGAAGAGT TCATGTCTCA ACCAGAGAAG CTCCTGTTTA TTATTGATGG CTTTGAGGAA ATAATCATAT CTGAGTCACG CTCTGAGAGC TTGGATGATG GCTCGCCATG TACAGACTGG TACCAGGAGC TCCCAGTGAC CAAAATCCTA CACAGCTTGT TGAAGAAAGA ATTGGTTCCC CTGGCTACCT TACTGATCAC GATCAAGACC TGGTTTGTGA GAGATCTTAA GGCCTCATTA GTGAATCCAT GCTTTGTACA AATTACAGGG TTCACAGGGG ACGACCTACG GGTATATTTC ATGAGACACT TTGATGACTC AAGTGAAGTT GAGAAAATCC TGCAGCAGCT AAGAAAAAAC GAAACTCTCT TTCATTCCTG CAGTGCCCCC ATGGTGTGTT GGACCGTATG TTCCTGTCTG AAGCAGCCGA AGGTGAGGTA TTACGATCTC CAGTCAATCA CTCAGACTAC CACCAGTCTG TATGCCTATT TTTTCTCCAA CTTGTTCTCC ACAGCAGAGG TAGATTTGGC AGATGACAGC TGGCCAGGAC AATGGAGGGC CCTCTGCAGT CTGGCCATAG AAGGGCTGTG GTCTATGAAC TTCACGTTTA ACAAAGAAGA CACTGAGATC GAGGGCCTGG AAGTGCCTTT CATTGATTCT CTCTACGAGT TCAATATTCT TCAAAAGATC AATGACTGTG GGGGTTGCAC TACTTTCACC CACCTAAGTT TCCAGGAGTT TTTTGCAGCC ATGTCCTTTG TGCTAGAGGA ACCTAGAGAA TTCCCTCCCC ATTCCACAAA GCCACAAGAG ATGAAGATGT TACTGCAACA CGTCTTGCTT GACAAAGAAG CCTACTGGAC TCCAGTGGTT CTGTTCTTCT TTGGTCTTTT AAATAAAAAC ATAGCAAGAG AACTGGAAGA TACTTTGCAT TGTAAAATAT CTCCCAGGGT AATGGAGGAA TTATTAAAGT GGGGAGAAGA GTTAGGTAAG GCTGAAAGTG CCTCTCTCCA ATTTCACATT CTACGACTTT TTCACTGCCT ACACGAGTCC CAGGAGGAAG ACTTCACAAA GAAGATGTTG GGTCGTATCT TTGAAGTTGA CCTTAATATT TTGGAGGACG AAGAACTCCA AGCTTCTTCA TTTTGCCTAA AGCACTGTAA AAGGTTAAAT AAGCTAAGGC TTTCTGTTAG CAGTCACATC CTTGAAAGGG ACTTGGAAAT TCTGGAGACA AGCAAGTTTG ATTCCAGGAT GCACGCATGG AACAGCATTT GCTCTACGTT GGTCACAAAT GAGAATCTGC ATGAGCTAGA CCTGAGTAAC AGCAAACTTC ATGCTTCCTC TGTGAAGGGT CTCTGTCTTG CACTGAAAAA TCCAAGATGC AAAGTCCAGA AACTGACGTG CAAATCGGTA ACTCCTGAGT GGGTTCTGCA GGACCTCATT ATTGCCCTTC AGGGTAACAG CAAGCTGACC CATCTGAACT TCAGCTCTAA CAAGCTGGGA ATGACTGTCC CCCTGATTCT TAAAGCTTTG AGACACTCAG CTTGCAACCT CAAGTATCTG TGCCTGGAGA AATGCAACTT GTCGGCAGCC AGCTGTCAGG ACCTAGCCTT GTTTCTCACC AGCATCCAAC ACGTAACTCG ATTGTGCCTG GGATTTAATC GGCTCCAAGA TGATGGCATA AAGCTATTGT GTGCGGCCCT GACTCACCCC AAGTGTGCCT TAGAGAGACT GGAGCTCTGG TTTTGCCAGC TGGCAGCACC CGCTTGCAAG CACTTGTCAG ATGCTCTCCT GCAGAACAGG AGCCTGACAC ACCTGAATCT GAGCAAGAAC AGCCTGAGAG ACGAGGGAGT CAAGTTCCTG TGTGAGGCCT TGGGTCGCCC AGATGGTAAC CTGCAGAGCC TGAATTTGTC AGGTTGTTCT TTCACAAGAG AGGGCTGTGG AGAGCTGGCT AATGCCCTCA GCCATAATCA TAATGTGAAA ATCTTGGATT TGGGAGAAAA TGATCTTCAG GATGATGGAG TGAAGCTACT GTGTGAGGCT CTGAAACCAC ATCGTGCATT GCACACACTT GGGTTGGCGA AATGCAATCT GACAACTGCT TGCTGCCAGC ATCTCTTCTC TGTTCTCAGC AGCAGTAAGA GCCTGGTCAA TCTGAACCTT CTAGGCAATG AATTGGATAC TGATGGTGTC AAGATGCTAT GTAAGGCTTT GAAAAAGTCG ACATGCAGGC TGCAGAAACT CGGGGGATCC GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT GACAAGTAGT GAGGGCCCGA TATCTCGACA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAGCTGAC GTCCTTTCCA TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCCT GGAAACGGGG GAGGCTAACT GAAACACGGA AGGAGACAAT ACCGGAAGGA ACCCGCGCTA TGACGGCAAT AAAAAGACAG AATAAAACGC ACGGGTGTTG GGTCGTTTGT TCATAAACGC GGGGTTCGGT CCCAGGGCTG GCACTCTGTC GATACCCCAC CGAGACCCCA TTGGGGCCAA TACGCCCGCG TTTCTTCCTT TTCCCCACCC CACCCCCCAA GTTCGGGTGA AGGCCCAGGG CTCGCAGCCA ACGTCGGGGC GGCAGGCCCT GCCATAGCAG ATCTGCGCAG CTGGGGCTCT AGGGGGTATC CCCACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG CATCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGGGGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTAATTCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCAAT GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCG ACGGATCGGG AGATCTCCCG ATCCCCTATG GTCGACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATC TGCTCCCTGC TTGTGTGTTG GAGGTCGCTG AGTAGTGCGC GAGCAAAATT TAAGCTACAA CAAGGCAAGG CTTGACCGAC AATTGCATGA AGAATCTGCT TAGG
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