PiggyBac-CMV-INSR(human)-Puro
$113.00
In stock
SKU
PL37039
NM_000208.4;CD220; HHF5
100μg liquid plasmid(Instantly):$134
| name | PiggyBac-CMV-INSR(human)-Puro | another name | aPiggyBac-CMV-INSR(human)-Puro |
| Insert Size (bp) | 4149bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression,Transpose | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG TACGTTAAAG ATAATCATGC GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAA CAAAAACTCA AAATTTCTTC TATAAAGTAA CAAAACTTTT ATGAGGGACA GCCCCCCCCC AAAGCCCCCA GGGATGTAAT TACGTCCCTC CCCCGCTAGG GGGCAGCAGC GAGCCGCCCG GGGCTCCGCT CCGGTCCGGC GCTCCCCCCG CATCCCCGAG CCGGCAGCGT GCGGGGACAG CCCGGGCACG GGGAAGGTGG CACGGGATCG CTTTCCTCTG AACGCTTCTC GCTGCTCTTT GAGCCTGCAG ACACCTGGGG GGATACGGGG AAAAGGCCTC CAAGGCCTAC TAGTATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT TTTGACCTCC ATAGAAGATT CTAGAGCTAG CGAATTCGCC ACCATGGCCA CCGGGGGCCG GCGGGGGGCG GCGGCCGCGC CGCTGCTGGT GGCGGTGGCC GCGCTGCTAC TGGGCGCCGC GGGCCACCTG TACCCCGGAG AGGTGTGTCC CGGCATGGAT ATCCGGAACA ACCTCACTAG GTTGCATGAG CTGGAGAATT GCTCTGTCAT CGAAGGACAC TTGCAGATAC TCTTGATGTT CAAAACGAGG CCCGAAGATT TCCGAGACCT CAGTTTCCCC AAACTCATCA TGATCACTGA TTACTTGCTG CTCTTCCGGG TCTATGGGCT CGAGAGCCTG AAGGACCTGT TCCCCAACCT CACGGTCATC CGGGGATCAC GACTGTTCTT TAACTACGCG CTGGTCATCT TCGAGATGGT TCACCTCAAG GAACTCGGCC TCTACAACCT GATGAACATC ACCCGGGGTT CTGTCCGCAT CGAGAAGAAC AATGAGCTCT GTTACTTGGC CACTATCGAC TGGTCCCGTA TCCTGGATTC CGTGGAGGAT AATTACATCG TGTTGAACAA AGATGACAAC GAGGAGTGTG GAGACATCTG TCCGGGTACC GCGAAGGGCA AGACCAACTG CCCCGCCACC GTCATCAACG GGCAGTTTGT CGAACGATGT TGGACTCATA GTCACTGCCA GAAAGTTTGC CCGACCATCT GTAAGTCACA CGGCTGCACC GCCGAAGGCC TCTGTTGCCA CAGCGAGTGC CTGGGCAACT GTTCTCAGCC CGACGACCCC ACCAAGTGCG TGGCCTGCCG CAACTTCTAC CTGGACGGCA GGTGTGTGGA GACCTGCCCG CCCCCGTACT ACCACTTCCA GGACTGGCGC TGTGTGAACT TCAGCTTCTG CCAGGACCTG CACCACAAAT GCAAGAACTC GCGGAGGCAG GGCTGCCACC AGTACGTCAT TCACAACAAC AAGTGCATCC CTGAGTGTCC CTCCGGGTAC ACGATGAATT CCAGCAACTT GCTGTGCACC CCATGCCTGG GTCCCTGTCC CAAGGTGTGC CACCTCCTAG AAGGCGAGAA GACCATCGAC TCGGTGACGT CTGCCCAGGA GCTCCGAGGA TGCACCGTCA TCAACGGGAG TCTGATCATC AACATTCGAG GAGGCAACAA TCTGGCAGCT GAGCTAGAAG CCAACCTCGG CCTCATTGAA GAAATTTCAG GGTATCTAAA AATCCGCCGA TCCTACGCTC TGGTGTCACT TTCCTTCTTC CGGAAGTTAC GTCTGATTCG AGGAGAGACC TTGGAAATTG GGAACTACTC CTTCTATGCC TTGGACAACC AGAACCTAAG GCAGCTCTGG GACTGGAGCA AACACAACCT CACCATCACT CAGGGGAAAC TCTTCTTCCA CTATAACCCC AAACTCTGCT TGTCAGAAAT CCACAAGATG GAAGAAGTTT CAGGAACCAA GGGGCGCCAG GAGAGAAACG ACATTGCCCT GAAGACCAAT GGGGACCAGG CATCCTGTGA AAATGAGTTA CTTAAATTTT CTTACATTCG GACATCTTTT GACAAGATCT TGCTGAGATG GGAGCCGTAC TGGCCCCCCG ACTTCCGAGA CCTCTTGGGG TTCATGCTGT TCTACAAAGA GGCCCCTTAT CAGAATGTGA CGGAGTTCGA CGGGCAGGAT GCGTGTGGTT CCAACAGTTG GACGGTGGTA GACATTGACC CACCCCTGAG GTCCAACGAC CCCAAATCAC AGAACCACCC AGGGTGGCTG ATGCGGGGTC TCAAGCCCTG GACCCAGTAT GCCATCTTTG TGAAGACCCT GGTCACCTTT TCGGATGAAC GCCGGACCTA TGGGGCCAAG AGTGACATCA TTTATGTCCA GACAGATGCC ACCAACCCCT CTGTGCCCCT GGATCCAATC TCAGTGTCTA ACTCATCATC CCAGATTATT CTGAAGTGGA AACCACCCTC CGACCCCAAT GGCAACATCA CCCACTACCT GGTTTTCTGG GAGAGGCAGG CGGAAGACAG TGAGCTGTTC GAGCTGGATT ATTGCCTCAA AGGGCTGAAG CTGCCCTCGA GGACCTGGTC TCCACCATTC GAGTCTGAAG ATTCTCAGAA GCACAACCAG AGTGAGTATG AGGATTCGGC CGGCGAATGC TGCTCCTGTC CAAAGACAGA CTCTCAGATC CTGAAGGAGC TGGAGGAGTC CTCGTTTAGG AAGACGTTTG AGGATTACCT GCACAACGTG GTTTTCGTCC CCAGAAAAAC CTCTTCAGGC ACTGGTGCCG AGGACCCTAG GCCATCTCGG AAACGCAGGT CCCTTGGCGA TGTTGGGAAT GTGACGGTGG CCGTGCCCAC GGTGGCAGCT TTCCCCAACA CTTCCTCGAC CAGCGTGCCC ACGAGTCCGG AGGAGCACAG GCCTTTTGAG AAGGTGGTGA ACAAGGAGTC GCTGGTCATC TCCGGCTTGC GACACTTCAC GGGCTATCGC ATCGAGCTGC AGGCTTGCAA CCAGGACACC CCTGAGGAAC GGTGCAGTGT GGCAGCCTAC GTCAGTGCGA GGACCATGCC TGAAGCCAAG GCTGATGACA TTGTTGGCCC TGTGACGCAT GAAATCTTTG AGAACAACGT CGTCCACTTG ATGTGGCAGG AGCCGAAGGA GCCCAATGGT CTGATCGTGC TGTATGAAGT GAGTTATCGG CGATATGGTG ATGAGGAGCT GCATCTCTGC GTCTCCCGCA AGCACTTCGC TCTGGAACGG GGCTGCAGGC TGCGTGGGCT GTCACCGGGG AACTACAGCG TGCGAATCCG GGCCACCTCC CTTGCGGGCA ACGGCTCTTG GACGGAACCC ACCTATTTCT ACGTGACAGA CTATTTAGAC GTCCCGTCAA ATATTGCAAA AATTATCATC GGCCCCCTCA TCTTTGTCTT TCTCTTCAGT GTTGTGATTG GAAGTATTTA TCTATTCCTG AGAAAGAGGC AGCCAGATGG GCCGCTGGGA CCGCTTTACG CTTCTTCAAA CCCTGAGTAT CTCAGTGCCA GTGATGTGTT TCCATGCTCT GTGTACGTGC CGGACGAGTG GGAGGTGTCT CGAGAGAAGA TCACCCTCCT TCGAGAGCTG GGGCAGGGCT CCTTCGGCAT GGTGTATGAG GGCAATGCCA GGGACATCAT CAAGGGTGAG GCAGAGACCC GCGTGGCGGT GAAGACGGTC AACGAGTCAG CCAGTCTCCG AGAGCGGATT GAGTTCCTCA ATGAGGCCTC GGTCATGAAG GGCTTCACCT GCCATCACGT GGTGCGCCTC CTGGGAGTGG TGTCCAAGGG CCAGCCCACG CTGGTGGTGA TGGAGCTGAT GGCTCACGGA GACCTGAAGA GCTACCTCCG TTCTCTGCGG CCAGAGGCTG AGAATAATCC TGGCCGCCCT CCCCCTACCC TTCAAGAGAT GATTCAGATG GCGGCAGAGA TTGCTGACGG GATGGCCTAC CTGAACGCCA AGAAGTTTGT GCATCGGGAC CTGGCAGCGA GAAACTGCAT GGTCGCCCAT GATTTTACTG TCAAAATTGG AGACTTTGGA ATGACCAGAG ACATCTATGA AACGGATTAC TACCGGAAAG GGGGCAAGGG TCTGCTCCCT GTACGGTGGA TGGCACCGGA GTCCCTGAAG GATGGGGTCT TCACCACTTC TTCTGACATG TGGTCCTTTG GCGTGGTCCT TTGGGAAATC ACCAGCTTGG CAGAACAGCC TTACCAAGGC CTGTCTAATG AACAGGTGTT GAAATTTGTC ATGGATGGAG GGTATCTGGA TCAACCCGAC AACTGTCCAG AGAGAGTCAC TGACCTCATG CGCATGTGCT GGCAATTCAA CCCCAAGATG AGGCCAACCT TCCTGGAGAT TGTCAACCTG CTCAAGGACG ACCTGCACCC CAGCTTTCCA GAGGTGTCGT TCTTCCACAG CGAGGAGAAC AAGGCTCCCG AGAGTGAGGA GCTGGAGATG GAGTTTGAGG ACATGGAGAA TGTGCCCCTG GACCGTTCCT CGCACTGTCA GAGGGAGGAG GCGGGGGGCC GGGATGGAGG GTCCTCGCTG GGTTTCAAGC GGAGCTACGA GGAACACATC CCTTACACAC ACATGAACGG AGGCAAGAAA AACGGGCGGA TTCTGACCTT GCCTCGGTCC AATCCTTCCT AAGCGGCCGC GAAGGATCTG CGATCGCTCC GGTGCCCGTC AGTGGGCAGA GCGCACATCG CCCACAGTCC CCGAGAAGTT GGGGGGAGGG GTCGGCAATT GAACGGGTGC CTAGAGAAGG TGGCGCGGGG TAAACTGGGA AAGTGATGTC GTGTACTGGC TCCGCCTTTT TCCCGAGGGT GGGGGAGAAC CGTATATAAG TGCAGTAGTC GCCGTGAACG TTCTTTTTCG CAACGGGTTT GCCGCCAGAA CACAGCTGAA GCTTCGAGGG GCTCGCATCT CTCCTTCACG CGCCCGCCGC CCTACCTGAG GCCGCCATCC ACGCCGGTTG AGTCGCGTTC TGCCGCCTCC CGCCTGTGGT GCCTCCTGAA CTGCGTCCGC CGTCTAGGTA AGTTTAAAGC TCAGGTCGAG ACCGGGCCTT TGTCCGGCGC TCCCTTGGAG CCTACCTAGA CTCAGCCGGC TCTCCACGCT TTGCCTGACC CTGCTTGCTC AACTCTACGT CTTTGTTTCG TTTTCTGTTC TGCGCCGTTA CAGATCCAAG CTGTGACCGG CGCCTACGCT AGACGCCACC ATGACCGAGT ACAAGCCCAC GGTGCGCCTC GCCACCCGCG ACGACGTCCC CAGGGCCGTA CGCACCCTCG CCGCCGCGTT CGCCGACTAC CCCGCCACGC GCCACACCGT CGATCCGGAC CGCCACATCG AGCGGGTCAC CGAGCTGCAA GAACTCTTCC TCACGCGCGT CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC GGACGACGGC GCCGCGGTGG CGGTCTGGAC CACGCCGGAG AGCGTCGAAG CGGGGGCGGT GTTCGCCGAG ATCGGCCCGC GCATGGCCGA GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA ACAGATGGAA GGCCTCCTGG CGCCGCACCG GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC CGTCGGCGTC TCGCCCGACC ACCAGGGCAA GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC CGCCTTCCTG GAGACCTCCG CGCCCCGCAA CCTCCCCTTC TACGAGCGGC TCGGCTTCAC CGTCACCGCC GACGTCGAGG TGCCCGAAGG ACCGCGCACC TGGTGCATGA CCCGCAAGCC CGGTGCCTGA AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCAGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGA ATTGACTCAA ATGATGTCAA TTAGTCTATC AGAAGCTATC TGGTCTCCCT TCCGGGGGAC AAGACATCCC TGTTTAATAT TTAAACAGCA GTGTTCCCAA ACTGGGTTCT TATATCCCTT GCTCTGGTCA ACCAGGTTGC AGGGTTTCCT GTCCTCACAG GAACGAAGTC CCTAAAGAAA CAGTGGCAGC CAGGTTTAGC CCCGGAATTG ACTGGATTCC TTTTTTAGGG CCCATTGGTA TGGCTTTTTC CCCGTATCCC CCCAGGTGTC TGCAGGCTCA AAGAGCAGCG AGAAGCGTTC AGAGGAAAGC GATCCCGTGC CACCTTCCCC GTGCCCGGGC TGTCCCCGCA CGCTGCCGGC TCGGGGATGC GGGGGGAGCG CCGGACCGGA GCGGAGCCCC GGGCGGCTCG CTGCTGCCCC CTAGCGGGGG AGGGACGTAA TTACATCCCT GGGGGCTTTG GGGGGGGGCT GTCCCTGATA TCTATAACAA GAAAATATAT ATATAATAAG TTATCACGTA AGTAGAACAT GAAATAACAA TATAATTATC GTATGAGTTA AATCTTAAAA GTCACGTAAA AGATAATCAT GCGTCATTTT GACTCACGCG GTCGTTATAG TTCAAAATCA GTGACACTTA CCGCATTGAC AAGCACGCCT CACGGGAGCT CCAAGCGGCG ACTGAGATGT CCTAAATGCA CAGCGACGGA TTCGCGCTAT TTAGAAAGAG AGAGCAATAT TTCAAGAATG CATGCGTCAA TTTTACGCAG ACTATCTTTC TAGGGTTAAT CTAGCTGCAT CAGGATCATA TCGTCGGGTC TTTTTTCCGG CTCAGTCATC GCCCAAGCTG GCGCTATCTG GGCATCGGGG AGGAAGAAGC CCGTGCCTTT TCCCGCGAGG TTGAAGCGGC ATGGAAAGAG TTTGCCGAGG ATGACTGCTG CTGCATTGAC GTTGAGCGAA AACGCACGTT TACCATGATG ATTCGGGAAG GTGTGGCCAT GCACGCCTTT AACGGTGAAC TGTTCGTTCA GGCCACCTGG GATACCAGTT CGTCGCGGCT TTTCCGGACA CAGTTCCGGA TGGTCAGCCC GAAGCGCATC AGCAACCCGA ACAATACCGG CGACAGCCGG AACTGCCGTG CCGGTGTGCA GATTAATGAC AGCGGTGCGG CGCTGGGATA TTACGTCAGC GAGGACGGGT ATCCTGGCTG GATGCCGCAG AAATGGACAT GGATACCCCG TGAGTTACCC GGCGGGCGCG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCAT
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