NM_153447.4;CLR19.8; MATER; NALP5; PAN11; PYPAF8
100μg liquid plasmid(Instantly):$120
| name | pCS2-6×Myc-NLRP5(human-repeat-opt) | another name | apCS2-6×Myc-NLRP5(human-repeat-opt) |
| Insert Size (bp) | 3600bp | Plasmid host | Mammary cell,Fish |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGCCATTCTG CCTGGGGACG TCGGAGCAAG CTTGATTTAG GTGACACTAT AGAATACAAG CTACTTGTTC TTTTTGCAGG ATCCCATCGA TTTAAAGCTA TGGAGCAAAA GCTCATTTCT GAAGAGGACT TGAATGAAAT GGAGCAAAAG CTCATTTCTG AAGAGGACTT GAATGAAATG GAGCAAAAGC TCATTTCTGA AGAGGACTTG AATGAAATGG AGCAAAAGCT CATTTCTGAA GAGGACTTGA ATGAAATGGA GCAAAAGCTC ATTTCTGAAG AGGACTTGAA TGAAATGGAG AGCTTGGGCG ACCTCACCAT GGAGCAAAAG CTCATTTCTG AAGAGGACTT GAATTCAAAG GTTGCAGGAG GACTTGAACT TGGAGCTGCT GCTCTGCTCT CAGCATCACC ACGTGCTCTT GTCACTCTTT CCACAGGTCC TACTTGCTCT ATATTACCAA AGAATCCACT TTTCCCCCAA AACCTGAGCT CTCAGCCTTG TATCAAGATG GAAGGAGACA AATCGCTCAC CTTTTCCAGC TACGGGCTGC AATGGTGTCT CTATGAGCTA GACAAGGAAG AATTTCAGAC ATTCAAGGAA TTACTAAAGA AGAAATCTTC AGAATCGACC ACATGCTCTA TTCCACAGTT TGAAATCGAG AATGCCAACG TGGAATGTCT GGCACTCCTC TTGCATGAGT ATTATGGAGC ATCGCTGGCC TGGGCTACGT CCATTAGCAT CTTTGAAAAC ATGAACCTGC GAACCCTCTC GGAGAAGGCA CGGGATGACA TGAAAAGACA TTCACCAGAA GATCCTGAAG CAACGATGAC TGACCAAGGA CCAAGCAAGG AAAAAGTGCC AGGAATTTCA CAGGCTGTGC AACAAGATAG TGCCACAGCT GCAGAGACAA AGGAGCAGGA GATCAGCCAG GCCATGGAGC AGGAAGGAGC AACAGCCGCC GAGACCGAAG AGCAGGAAAT TTCACAAGCT ATGGAACAAG AAGGTGCCAC AGCAGCAGAG ACAGAAGAAC AAGGACATGG AGGTGACACA TGGGACTACA AGAGTCACGT GATGACCAAA TTCGCTGAGG AGGAGGATGT ACGTCGTAGT TTTGAAAACA CTGCTGCTGA CTGGCCGGAA ATGCAAACGT TGGCTGGTGC TTTTGATTCA GACCGGTGGG GCTTCCGGCC TCGCACGGTG GTTCTGCACG GAAAGTCAGG AATTGGGAAA TCGGCTCTAG CCAGAAGGAT CGTGCTGTGC TGGGCGCAAG GTGGACTCTA CCAGGGAATG TTCTCCTACG TCTTCTTCCT CCCCGTTAGA GAGATGCAGC GGAAGAAGGA GAGCAGTGTC ACAGAGTTCA TCTCCAGGGA GTGGCCAGAC TCCCAGGCTC CGGTGACGGA GATCATGTCC CGACCAGAAA GGCTGTTGTT CATCATTGAC GGTTTCGATG ACCTGGGCTC TGTCCTCAAC AATGACACAA AGCTCTGCAA AGACTGGGCT GAGAAGCAGC CTCCGTTCAC CCTCATACGC AGTCTGCTGA GGAAGGTCCT GCTCCCTGAG TCCTTCCTGA TCGTCACCGT CAGAGACGTG GGCACAGAGA AGCTCAAGTC AGAGGTCGTG TCTCCCCGTT ACCTGTTAGT TAGAGGAATC TCCGGGGAAC AAAGAATCCA CTTGCTCCTT GAGCGCGGGA TTGGTGAGCA TCAGAAGACA CAAGGGTTGC GTGCGATCAT GAACAACCGT GAGCTGCTCG ACCAGTGCCA GGTGCCCGCC GTGGGCTCTC TCATCTGCGT GGCCCTGCAG CTGCAGGACG TGGTGGGGGA GAGCGTCGCC CCCTTCAACC AAACGCTCAC AGGCCTGCAC GCCGCTTTTG TGTTTCATCA GCTCACCCCT CGAGGCGTGG TCCGGCGCTG TCTCAATCTG GAGGAAAGAG TTGTCCTGAA GCGCTTCTGC CGTATGGCTG TGGAGGGAGT GTGGAATAGG AAGTCAGTGT TTGACGGTGA CGACCTCATG GTTCAAGGAC TCGGGGAGTC TGAGCTCCGT GCTCTGTTTC ACATGAACAT CCTTCTCCCA GACAGCCACT GTGAGGAGTA CTACACCTTC TTCCACCTCA GTCTCCAGGA CTTCTGTGCC GCCTTGTACT ACGTGTTAGA GGGCCTGGAA ATCGAGCCAG CTCTCTGCCC TCTGTACGTT GAGAAGACAA AGAGGTCCAT GGAGCTTAAA CAGGCAGGCT TCCATATCCA CTCGCTTTGG ATGAAGCGTT TCTTGTTTGG CCTCGTGAGC GAAGACGTAA GGAGGCCACT GGAGGTCCTG CTGGGCTGTC CCGTTCCCCT GGGGGTGAAG CAGAAGCTTC TGCACTGGGT CTCTCTGTTG GGTCAGCAGC CTAATGCCAC CACCCCAGGA GACACCCTGG ACGCCTTCCA CTGTCTTTTC GAGACTCAAG ACAAAGAGTT TGTTCGCTTG GCATTAAACA GCTTCCAAGA AGTGTGGCTT CCGATTAACC AGAACCTGGA CTTGATAGCA TCTTCCTTCT GCCTCCAGCA CTGTCCGTAT TTGCGGAAAA TTCGGGTGGA TGTCAAAGGG ATCTTCCCAA GAGATGAGTC CGCTGAGGCA TGTCCTGTGG TCCCTCTATG GATGCGGGAT AAGACCCTCA TTGAGGAGCA GTGGGAAGAT TTCTGCTCCA TGCTTGGCAC CCACCCACAC CTGCGGCAGC TGGACCTGGG CAGCAGCATC CTGACAGAGC GGGCCATGAA GACCCTGTGT GCCAAGCTGA GGCATCCCAC CTGCAAGATA CAGACCCTGA TGTTTAGAAA TGCACAGATT ACCCCTGGTG TGCAGCACCT CTGGAGAATC GTCATGGCCA ACCGTAACCT AAGATCCCTC AACTTGGGAG GCACCCACCT GAAGGAAGAG GATGTAAGGA TGGCGTGTGA AGCCTTAAAA CACCCAAAAT GTTTGTTGGA GTCTTTGAGG CTGGATTGCT GTGGATTGAC CCATGCCTGT TACCTGAAGA TCTCCCAAAT CCTTACGACC TCCCCCAGCC TGAAATCTCT GAGCCTGGCA GGAAACAAGG TGACAGACCA GGGAGTAATG CCTCTCAGTG ATGCCTTGAG AGTCTCCCAG TGCGCCCTGC AGAAGCTGAT ACTGGAGGAC TGTGGCATCA CAGCCACGGG TTGCCAGAGT CTGGCCTCAG CCCTCGTCAG CAACCGGAGC TTGACACACC TGTGCCTATC CAACAACAGC CTGGGGAACG AAGGTGTAAA TCTACTGTGT CGATCCATGA GGCTTCCCCA CTGTAGTCTG CAGAGGCTTA TGCTGAATCA GTGCCACCTG GACACGGCTG GCTGTGGTTT TCTTGCACTT GCGCTTATGG GTAACTCATG GCTGACGCAC CTGAGCCTTA GCATGAACCC TGTGGAAGAC AATGGCGTGA AGCTTCTGTG CGAGGTCATG AGAGAACCAT CTTGTCATCT CCAGGACCTG GAGTTGGTAA AGTGTCATCT CACCGCCGCG TGCTGTGAGA GTCTGTCCTG TGTGATCTCG AGGAGCAGAC ACCTGAAGAG CCTGGATCTC ACGGACAATG CCCTGGGTGA CGGTGGGGTT GCTGCGCTGT GCGAGGGACT GAAGCAAAAG AACAGTGTTC TGGCGAGACT CGGGTTGAAG GCATGTGGAC TGACTTCTGA TTGCTGTGAG GCACTCTCCT TGGCCCTTTC CTGCAACCGG CATCTGACCA GTCTAAACCT GGTGCAGAAT AACTTCAGTC CCAAAGGAAT GATGAAGCTG TGTTCGGCCT TTGCCTGTCC CACGTCTAAC TTACAGATAA TTGGGCTGTG GAAATGGCAG TACCCTGTGC AAATAAGGAA GCTGCTGGAG GAAGTGCAGC TACTCAAGCC CCGAGTCGTA ATTGACGGTA GTTGGCATTC TTTTGATGAA GATGACCGGT ACTGGTGGAA AAACTGATCT AGAACTATAG TGAGTCGTAT TACGTAGATC CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAATTCGCG GCCGCGGCGC CAATGCATTG GGCCCGGTAC CCAGCTTTTG TTCCCTTTAG TGAGGGTTAA TTGCGCGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTAAATTG TAAGCGTTAA TATTTTGTTA AAATTCGCGT TAAATTTTTG TTAAATCAGC TCATTTTTTA ACCAATAGGC CGAAATCGGC AAAATCCCTT ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTGTTGT TCCAGTTTGG AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA AACCGTCTAT CAGGGCGATG GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGGG GTCGAGGTGC CGTAAAGCAC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA TGCGCCGCTA CAGGGCGCGT CCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GTCGACCATA GCCAATTCAA TATGGCGTAT ATGGACTCAT GCCAATTCAA TATGGTGGAT CTGGACCTGT GCCAATTCAA TATGGCGTAT ATGGACTCGT GCCAATTCAA TATGGTGGAT CTGGACCCCA GCCAATTCAA TATGGCGGAC TTGGCACCAT GCCAATTCAA TATGGCGGAC TTGGCACTGT GCCAACTGGG GAGGGGTCTA CTTGGCACGG TGCCAAGTTT GAGGAGGGGT CTTGGCCCTG TGCCAAGTCC GCCATATTGA ATTGGCATGG TGCCAATAAT GGCGGCCATA TTGGCTATAT GCCAGGATCA ATATATAGGC AATATCCAAT ATGGCCCTAT GCCAATATGG CTATTGGCCA GGTTCAATAC TATGTATTGG CCCTATGCCA TATAGTATTC CATATATGGG TTTTCCTATT GACGTAGATA GCCCCTCCCA ATGGGCGGTC CCATATACCA TATATGGGGC TTCCTAATAC CGCCCATAGC CACTCCCCCA TTGACGTCAA TGGTCTCTAT ATATGGTCTT TCCTATTGAC GTCATATGGG CGGTCCTATT GACGTATATG GCGCCTCCCC CATTGACGTC AATTACGGTA AATGGCCCGC CTGGCTCAAT GCCCATTGAC GTCAATAGGA CCACCCACCA TTGACGTCAA TGGGATGGCT CATTGCCCAT TCATATCCGT TCTCACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC ACTTGGCAGT ACATCAATAT CTATTAATAG TAACTTGGCA AGTACATTAC TATTGGAAGG ACGCCAGGGT ACATTGGCAG TACTCCCATT GACGTCAATG GCGGTAAATG GCCCGCGATG GCTGCCAAGT ACATCCCCAT TGACGTCAAT GGGGAGGGGC AATGACGCAA ATGGGCGTTC CATTGACGTA AATGGGCGGT AGGCGTGCCT AATGGGAGGT CTATATAAGC AATGCTCGTT TAGGGAAC
