pAG1-H3
$70.00
In stock
SKU
PL36995
100μg liquid plasmid(Instantly):$113
| name | pAG1-H3 | another name | apAG1-H3 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | Replicon | oriV | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | Low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CAAATTGACG CTTAGACAAC TTAATAACAC ATTGCGGACG TTTTTAATGT ACTGGGGTGG TTTTTCTTTT CACCAGTGAG ACGGGCAACA GCGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGAATTCG AGCTCGGTAC CAAGGCCCGG GCTGGCCACG GCCGCCTAGG CGCGCAAGGA TCCTCTAGAT CTCGAGGCCT GATCATCGAT GGGCCCATCG ATGATCAGGC CTCGACAGAA GATGATATTG AAGGAGCACT TTTTGGGCTT GGCTGGAGCT AGTGGAGGTC AACAATGAAT GCCTATTTTG GTTTAGTCGT CCAGGCGGTG AGCACAAAAT TTGTGTCGTT TGACAAGATG GTTCATTTAG GCAACTGGTC AGATCAGCCC CACTTGTAGC AGTAGCGGCG GCGCTCGAAG TGTGACTCTT ATTAGCAGAC AGGAACGAGG ACATTATTAT CATCTGCTGC TTGGTGCACG ATAACTTGGT GCGTTTGTCA AGCAAGGTAA GTGAACGACC CGGTCATACC TTCTTAAGTT CGCCCTTCCT CCCTTTATTT CAGATTCAAT CTGACTTACC TATTCTACCC AAGCATCGAT ATGAAAAAGC CTGAACTCAC CGCGACGTCT GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG ACCTGATGCA GCTCTCGGAG GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC GTGGATATGT CCTGCGGGTA AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT TGCATCGGCC GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA GCGAGAGCCT GACCTATTGC ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC CTGAAACCGA ACTGCCCGCT GTTCTGCAGC CGGTCGCGGA GGCCATGGAT GCGATCGCTG CGGCCGATCT TAGCCAGACG AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG GCGTGATTTC ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA CGACACCGTC AGTGCGTCCG TCGCGCAGGC TCTCGATGAG CTGATGCTTT GGGCCGAGGA CTGCCCCGAA GTCCGGCACC TCGTGCACGC GGATTTCGGC TCCAACAATG TCCTGACGGA CAATGGCCGC ATAACAGCGG TCATTGACTG GAGCGAGGCG ATGTTCGGGG ATTCCCAATA CGAGGTCGCC AACATCTTCT TCTGGAGGCC GTGGTTGGCT TGTATGGAGC AGCAGACGCG CTACTTCGAG CGGAGGCATC CGGAGCTTGC AGGATCGCCG CGGCTCCGGG CGTATATGCT CCGCATTGGT CTTGACCAAC TCTATCAGAG CTTGGTTGAC GGCAATTTCG ATGATGCAGC TTGGGCGCAG GGTCGATGCG ACGCAATCGT CCGATCCGGA GCCGGGACTG TCGGGCGTAC ACAAATCGCC CGCAGAAGCG CGGCCGTCTG GACCGATGGC TGTGTAGAAG TACTCGCCGA TAGTGGAAAC CGACGCCCCA GCACTCGTCC GAGGGCAAAG GAATAGAGTA GATGCCGACC GGGATCCACT TAACGTTACT GAAATCATCA AACAGCTTGA CGAATCTGGA TATAAGATCG TTGGTGTCGA TGTCAGCTCC GGAGTTGAGA CAAATGGTGT TCAGGATCTC GATAAGATAC GTTCATTTGT CCAAGCAGCA AAGAGTGCCT TCTAGTGATT TAATAGCTCC ATGTCAACAA GAATAAAACG CGTTTCGGGT TTACCTCTTC CAGATACAGC TCATCTGCAA TGCATTAATG CATTGGACCT CGCAACCCTA GTACGCCCTT CAGGCTCCGG CGAAGCAGAA GAATAGCTTA GCAGAGTCTA TTTTCATTTT CGGGAGACGA GATCAAGCAG ATCAACGGTC GTCAAGAGAC CTACGAGACT GAGGAATCCG CTCTTGGCTC CACGCGACTA TATATTTGTC TCTAATTGTA CTTTGACATG CTCCTCTTCT TTACTCTGAT AGCTTGACTA TGAAAATTCC GTCACCAGCC CCTGGGTTCG CAAAGATAAT TGCACTGTTT CTTCCTTGAA CTCTCAAGCC TACAGGACAC ACATTCATCG TAGGTATAAA CCTCGAAAAT CATTCCTACT AAGATGGGTA TACAATAGTA ACCATGGTTG CCTAGTGAAT GCTCCGTAAC ACCCAATACG CCGGCCGAAA CTTTTTTACA ACTCTCCTAT GAGTCGTTTA CCCAGAATGC ACAGGTACAC TTGTTTAGAG GTAATCCTTC TTTCTAGAGG ATCCTCTACG CCGGACGCAT CGTGGCCGGC ATCACCGGCG CCACAGGTGC GGTTGCTGGC GCCTATATCG CCGACATCAC CGATGGGGAA GATCGGGCTC GCCACTTCGG GCTCATGAGC GCTTGTTTCG GCGTGGGTAT GGTGGCAGGC CCCGTGGCCG GGGGACTGTT GGGCGCCATC TCCTTGCATG CACCATTCCT TGCGGCGGCG GTGCTCAACG GCCTCAACCT ACTACTGGGC TGCTTCCTAA TGCAGGAGTC GCATAAGGGA GAGCGTCGAG ATCTAGAGGA TCCCCCGACT AGTGCGCGAT CGCGGCCGGC CGGCGCGCCG TTTAAACGGA TTTAAATTAA TTAATGTCGA CCTGCAGGCA TGCAAGCTTC GTGACTCCCT TAATTCTCCG CTCATGATCA GATTGTCGTT TCCCGCCTTC AGTTTAAACT ATCAGTGTTT GACAGGATAT ATTGGCGGGT AAACCTAAGA GAAAAGAGCG TTTATTAGAA TAATCGGATA TTTAAAAGGG CGTGAAAAGG TTTATCCGTT CGTCCATTTG TTTGTTCATG CCAACCACAG GGTTCCAGAT CCGACGAGCA AGGCAAGACC GAGCGCCTTT GCGACGCTCA CCGGGCTGGT TGCCCTCGCC GCTGGGCTGG CGGCCGTCTA TGGCCCTGCA AACGCGCCAG AAACGCCGTC GAAGCCGTGT GCGAGACACC GCGGCCGCCG GCGTTGTGGA TACCTCGCGG AAAACTTGGC CCTCACTGAC AGATGAGGGG CGGACGTTGA CACTTGAGGG GCCGACTCAC CCGGCGCGGC GTTGACAGAT GAGGGGCAGG CTCGATTTCG GCCGGCGACG TGGAGCTGGC CAGCCTCGCA AATCGGCGAA AACGCCTGAT TTTACGCGAG TTTCCCACAG ATGATGTGGA CAAGCCTGGG GATAAGTGCC CTGCGGTATT GACACTTGAG GGGCGCGACT ACTGACAGAT GAGGGGCGCG ATCCTTGACA CTTGAGGGGC AGAGTGCTGA CAGATGAGGG GCGCACCTAT TGACATTTGA GGGGCTGTCC ACAGGCAGAA AATCCAGCAT TTGCAAGGGT TTCCGCCCGT TTTTCGGCCA CCGCTAACCT GTCTTTTAAC CTGCTTTTAA ACCAATATTT ATAAACCTTG TTTTTAACCA GGGCTGCGCC CTGTGCGCGT GACCGCGCAC GCCGAAGGGG GGTGCCCCCC CTTCTCGAAC CCTCCCGGCC CGCTAACGCG GGCCTCCCAT CCCCCCAGGC GTACGCCACT GGAGCACCTC AAAAACACCA TCATACACTA AATCAGTAAG TTGGCAGCAT CACCCATAAT TGTGGTTTCA AAATCGGCTC CGTCGATACT ATGTTATACG CCAACTTTGA AAACAACTTT GAAAAAGCTG TTTTCTGGTA TTTAAGGTTT TAGAATGCAA GGAACAGTGA ATTGGAGTTC GTCTTGTTAT AATTAGCTTC TTGGGGTATC TTTAAATACT GTAGAAAAGA GGAAGGAAAT AATAAATGGC TAAAATGAGA ATATCACCGG AATTGAAAAA ACTGATCGAA AAATACCGCT GCGTAAAAGA TACGGAAGGA ATGTCTCCTG CTAAGGTATA TAAGCTGGTG GGAGAAAATG AAAACCTATA TTTAAAAATG ACGGACAGCC GGTATAAAGG GACCACCTAT GATGTGGAAC GGGAAAAGGA CATGATGCTA TGGCTGGAAG GAAAGCTGCC TGTTCCAAAG GTCCTGCACT TTGAACGGCA TGATGGCTGG AGCAATCTGC TCATGAGTGA GGCCGATGGC GTCCTTTGCT CGGAAGAGTA TGAAGATGAA CAAAGCCCTG AAAAGATTAT CGAGCTGTAT GCGGAGTGCA TCAGGCTCTT TCACTCCATC GACATATCGG ATTGTCCCTA TACGAATAGC TTAGACAGCC GCTTAGCCGA ATTGGATTAC TTACTGAATA ACGATCTGGC CGATGTGGAT TGCGAAAACT GGGAAGAAGA CACTCCATTT AAAGATCCGC GCGAGCTGTA TGATTTTTTA AAGACGGAAA AGCCCGAAGA GGAACTTGTC TTTTCCCACG GCGACCTGGG AGACAGCAAC ATCTTTGTGA AAGATGGCAA AGTAAGTGGC TTTATTGATC TTGGGAGAAG CGGCAGGGCG GACAAGTGGT ATGACATTGC CTTCTGCGTC CGGTCGATCA GGGAGGATAT CGGGGAAGAA CAGTATGTCG AGCTATTTTT TGACTTACTG GGGATCAAGC CTGATTGGGA GAAAATAAAA TATTATATTT TACTGGATGA ATTGTTTTAG TACCTAGATG TGGCGCAACG ATGCCGGCGA CAAGCAGGAG CGCACCGACT TCTTCCGCAT CAAGTGTTTT GGCTCTCAGG CCGAGGCCCA CGGCAAGTAT TTGGGCAAGG GGTCGCTGGT ATTCGTGCAG GGCAAGATTC GGAATACCAA GTACGAGAAG GACGGCCAGA CGGTCTACGG GACCGACTTC ATTGCCGATA AGGTGGATTA TCTGGACACC AAGGCACCAG GCGGGTCAAA TCAGGAATAA GGGCACATTG CCCCGGCGTG AGTCGGGGCA ATCCCGCAAG GAGGGTGAAT GAATCGGACG TTTGACCGGA AGGCATACAG GCAAGAACTG ATCGACGCGG GGTTTTCCGC CGAGGATGCC GAAACCATCG CAAGCCGCAC CGTCATGCGT GCGCCCCGCG AAACCTTCCA GTCCGTCGGC TCGATGGTCC AGCAAGCTAC GGCCAAGATC GAGCGCGACA GCGTGCAACT GGCTCCCCCT GCCCTGCCCG CGCCATCGGC CGCCGTGGAG CGTTCGCGTC GTCTCGAACA GGAGGCGGCA GGTTTGGCGA AGTCGATGAC CATCGACACG CGAGGAACTA TGACGACCAA GAAGCGAAAA ACCGCCGGCG AGGACCTGGC AAAACAGGTC AGCGAGGCCA AGCAGGCCGC GTTGCTGAAA CACACGAAGC AGCAGATCAA GGAAATGCAG CTTTCCTTGT TCGATATTGC GCCGTGGCCG GACACGATGC GAGCGATGCC AAACGACACG GCCCGCTCTG CCCTGTTCAC CACGCGCAAC AAGAAAATCC CGCGCGAGGC GCTGCAAAAC AAGGTCATTT TCCACGTCAA CAAGGACGTG AAGATCACCT ACACCGGCGT CGAGCTGCGG GCCGACGATG ACGAACTGGT GTGGCAGCAG GTGTTGGAGT ACGCGAAGCG CACCCCTATC GGCGAGCCGA TCACCTTCAC GTTCTACGAG CTTTGCCAGG ACCTGGGCTG GTCGATCAAT GGCCGGTATT ACACGAAGGC CGAGGAATGC CTGTCGCGCC TACAGGCGAC GGCGATGGGC TTCACGTCCG ACCGCGTTGG GCACCTGGAA TCGGTGTCGC TGCTGCACCG CTTCCGCGTC CTGGACCGTG GCAAGAAAAC GTCCCGTTGC CAGGTCCTGA TCGACGAGGA AATCGTCGTG CTGTTTGCTG GCGACCACTA CACGAAATTC ATATGGGAGA AGTACCGCAA GCTGTCGCCG ACGGCCCGAC GGATGTTCGA CTATTTCAGC TCGCACCGGG AGCCGTACCC GCTCAAGCTG GAAACCTTCC GCCTCATGTG CGGATCGGAT TCCACCCGCG TGAAGAAGTG GCGCGAGCAG GTCGGCGAAG CCTGCGAAGA GTTGCGAGGC AGCGGCCTGG TGGAACACGC CTGGGTCAAT GATGACCTGG TGCATTGCAA ACGCTAGGGC CTTGTGGGGT CAGTTCCGGC TGGATCTGCT CTCCCGCTGA CGCCGTCCCG GACTGATGGG CTGCCTGTAT CGAGTGGTGA TTTTGTGCCG AGCTGCCGGT CGGGGAGCTG TTGGCTGGCT GGTGGCAGGA TATATTGTGG TGTAAA
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