pLV3-CMV-Fbn1(mouse)-3×HA-CopGFP-Puro
$171.00
In stock
SKU
PL36979
NM_007993.2;B430209H23; Fib-1; Tsk
100μg liquid plasmid(Instantly):$192
| name | pLV3-CMV-Fbn1(mouse)-3×HA-CopGFP-Puro | another name | apLV3-CMV-Fbn1(mouse)-3×HA-CopGFP-Puro |
| Insert Size (bp) | 8619bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT TATCGATACT AGTATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTTT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGATTC TAGAGCTAGC CTGATACGAA CTCGGAATTC GCCACCATGA TGCGGCGAGG AGGGCTGCTG GAGGTCGCGC TGGCGTTCGC CCTGCTCCTC GAGTCCTACA CGAGCCATGG GGCGGACGCC AATTTGGAGG CTGGGAGCCT GAAGGAGACC AGAGCCAATC GGGCCAAGAG AAGAGGCGGC GGAGGACACG ATGCGCTGAA AGGACCCAAT GTCTGTGGAT CACGTTATAA TGCATACTGT TGTCCTGGAT GGAAAACCTT ACCTGGTGGA AATCAGTGTA TTGTTCCCAT TTGCCGGCAT TCCTGTGGGG ATGGATTCTG CTCGAGGCCA AATATGTGCA CTTGCCCGTC TGGTCAGATA TCTCCTTCCT GTGGCTCCAG ATCCATCCAA CACTGCAGCA TCCGCTGTAT GAATGGGGGC AGCTGCAGCG ATGACCACTG TCTGTGCCAG AAAGGGTACA TCGGCACTCA CTGTGGACAG CCTGTCTGTG AAAGTGGCTG TCTCAACGGA GGGAGGTGTG TGGCCCCAAA TCGGTGTGCT TGCACGTACG GCTTTACTGG ACCCCAGTGT GAAAGAGATT ACAGAACAGG CCCATGTTTT ACTGTGGTAA GCAACCAGAT GTGCCAGGGA CAGCTCAGCG GGATTGTCTG CACCAAAACA CTTTGCTGTG CCACCGTGGG CCGAGCCTGG GGCCACCCCT GTGAGATGTG TCCTGCCCAG CCTCACCCCT GCCGCCGCGG CTTCATTCCC AACATCCGCA CTGGAGCTTG TCAAGATGTG GATGAATGTC AGGCCATCCC AGGGATGTGT CAAGGAGGAA ATTGCATTAA TACCGTTGGA TCTTTTGAGT GCAAATGCCC TGCTGGACAC AAATTTAATG AAGTGTCACA AAAATGTGAA GATATTGACG AGTGCAGCAC CATTCCTGGA GTCTGCGATG GCGGGGAATG TACAAACACT GTCAGCAGCT ACTTCTGCAA ATGTCCCCCT GGTTTTTACA CCTCTCCTGA TGGCACCAGA TGCGTAGATG TTCGCCCTGG TTACTGCTAC ACAGCTCTGG CAAACGGGCG CTGCTCTAAC CAGCTGCCAC AGTCCATAAC CAAAATGCAG TGCTGTTGCG ATCTTGGCCG GTGCTGGTCT CCAGGGGTTA CTGTTGCTCC CGAGATGTGT CCCATCAGGT CAACTGAGGA TTTCAACAAG CTGTGCTCTG TCCCTCTGGT AATTCCCGGG AGACCAGAAT ATCCTCCCCC ACCCATTGGC CCCCTTCCTC CAGTTCAGCC CGTTCCTCCT GGCTATCCTC CTGGGCCTGT GATTCCAGCC CCTCGGCCAC CGCCAGAATA TCCATATCCA TCTCCGTCTC GGGAACCACC AAGGGTGCTG CCTTTCAACG TTACTGACTA CTGTCAACTG GTCCGCTATC TCTGTCAAAA TGGGCGCTGC ATTCCAACTC CCGGTAGCTA CCGCTGCGAG TGCAACAAGG GCTTCCAGCT GGATATCCGT GGCGAATGCA TCGACGTGGA TGAGTGTGAG AAGAACCCAT GCACTGGTGG CGAGTGCATC AACAACCAGG GCTCCTACAC CTGTCACTGC AGAGCTGGCT ACCAGAGCAC ACTCACCAGA ACTGAGTGCA GAGACATAGA TGAGTGTCTT CAGAATGGCC GGATCTGCAA CAATGGTCGC TGTATCAACA CAGACGGCAG CTTCCACTGC GTATGCAATG CGGGCTTTCA TGTCACGCGG GACGGAAAGA ACTGTGAAGA TATGGATGAG TGCAGCATCC GAAACATGTG CCTAAACGGA ATGTGTATTA ATGAAGATGG CAGTTTCAAG TGTATTTGCA AACCTGGGTT CCAACTGGCA TCAGATGGCC GCTACTGCAA AGATATCAAT GAGTGTGAGA CACCTGGGAT CTGCATGAAC GGACGCTGTG TGAACACGGA TGGCTCCTAC AGATGCGAAT GCTTCCCCGG ATTGGCTGTG GGTCTAGACG GACGTGTGTG TGTTGACACA CACATGCGGA GCACATGCTA TGGAGGATAC AGGAGAGGCC AGTGCGTGAA GCCGTTGTTT GGTGCTGTTA CCAAATCGGA ATGCTGTTGT GCCAGCACTG AGTATGCCTT TGGGGAACCC TGCCAGCCGT GTCCTGCACA GAATTCAGCG GAATATCAGG CACTCTGCAG CAGTGGACCG GGAATGACAT CAGCAGGCAC TGATATAAAC GAATGTGCAT TAGATCCTGA TATTTGCCCA AATGGAATTT GTGAAAATCT CCGTGGGACC TACAAATGTA TATGCAACTC GGGATATGAA GTAGACATAA CTGGGAAAAA CTGTGTCGAT ATTAATGAGT GTGTGCTGAA CAGTCTACTT TGTGACAATG GACAATGTCG AAACACACCT GGAAGTTTTG TCTGCACCTG CCCCAAAGGA TTTGTGTACA AACCTGACCT AAAAACCTGT GAAGACATTG ATGAATGTGA ATCGAGTCCT TGCATTAATG GAGTCTGCAA GAACAGCCCT GGCTCCTTCA TTTGTGAATG TTCTCCTGAA AGTACTCTGG ACCCAACAAA AACCATCTGC ATAGAAACCA TCAAGGGCAC TTGCTGGCAG ACTGTCATCG ACGGGCGCTG TGAGATCAAC ATCAACGGAG CCACCTTGAA GTCCGAGTGC TGCTCCTCCC TTGGTGCTGC GTGGGGGAGC CCGTGCACCA TCTGTCAACT TGATCCCATT TGTGGTAAAG GGTTCTCAAG AATTAAAGGC ACGCAATGTG AAGATATCAA TGAGTGTGAA GTGTTCCCGG GAGTATGCAA GAACGGCCTG TGTGTCAACT CCAGGGGTTC ATTCAAGTGC GAGTGTCCCA ATGGAATGAC TTTGGATGCT ACAGGAAGAA TCTGTCTTGA CATCCGCCTG GAGACCTGCT TCCTCAAGTA TGACGATGAA GAGTGCACCT TGCCCATCGC TGGCCGCCAC CGAATGGATG CCTGCTGCTG CTCTGTTGGG GCAGCCTGGG GAACGGAAGA GTGTGAGGAG TGTCCATTGA GAAACAGCCG GGAGTATGAG GAACTCTGTC CCCGAGGACC TGGGTTTGCC ACAAAAGACA TTACAAATGG GAAACCTTTC TTCAAAGATA TCAATGAGTG CAAGATGATA CCCAGCCTCT GTACCCACGG CAAGTGCAGG AACACCATTG GCAGCTTCAA GTGTAGGTGT GACAGTGGCT TTGCTCTGGA TTCTGAAGAG AGGAACTGTA CAGACATTGA TGAGTGCCGC ATATCTCCTG ACCTCTGTGG CCGAGGCCAG TGTGTGAACA CCCCGGGGGA CTTTGAATGC AAGTGTGATG AAGGCTATGA AAGTGGCTTC ATGATGATGA AGAACTGCAT GGATATTGAT GAATGTCAGA GAGATCCTCT CCTGTGTCGA GGAGGCATTT GCCACAACAC AGAGGGAAGC TATCGCTGCG AATGTCCTCC TGGTCACCAA TTGTCCCCAA ACATCTCTGC ATGCATTGAC ATCAACGAGT GTGAGCTGAG TGCGAATCTC TGTCCCCATG GGCGTTGTGT GAACCTCATA GGGAAGTACC AGTGTGCCTG CAACCCTGGC TACCACCCCA CTCATGACAG GCTCTTCTGT GTCGATATTG ATGAATGCAG CATAATGAAC GGTGGTTGTG AGACCTTCTG CACAAACTCT GACGGGAGCT ATGAATGTAG CTGTCAGCCA GGCTTCGCGC TAATGCCAGA CCAGCGATCG TGCACAGACA TTGATGAGTG TGAAGACAAC CCCAATATCT GTGATGGTGG CCAGTGCACA AACATACCTG GGGAGTACAG GTGCCTGTGC TATGATGGGT TCATGGCATC TGAAGACATG AAGACTTGTG TGGATGTCAA TGAGTGTGAC CTGAATCCAA ACATCTGCCT TAGTGGGACC TGTGAAAATA CTAAAGGCTC GTTCATCTGC CACTGTGATA TGGGATATTC AGGGAAGAAA GGAAAAACGG GCTGTACAGA TATCAATGAA TGTGAGATCG GAGCACACAA CTGTGGCAGA CATGCTGTAT GCACAAATAC AGCCGGGAGC TTCAAGTGCA GCTGCAGTCC CGGCTGGATT GGAGACGGCA TTAAGTGCAC AGATCTGGAT GAATGCTCTA ATGGAACCCA CATGTGCAGC CAACACGCGG ACTGCAAGAA CACCATGGGG TCATATCGCT GTCTCTGTAA GGATGGCTAT ACAGGGGATG GCTTCACCTG TACAGACCTC GACGAGTGCT CCGAGAACCT GAACCTCTGT GGCAATGGCC AGTGCCTCAA CGCCCCTGGC GGGTACCGCT GTGAATGCGA CATGGGCTTC GTGCCCAGTG CTGACGGGAA GGCCTGTGAA GATATCGATG AGTGCTCCCT TCCAAACATC TGTGTCTTTG GAACTTGCCA CAACCTCCCG GGCCTCTTCC GTTGCGAGTG TGAGATTGGC TATGAACTGG ACCGAAGTGG TGGAAACTGC ACAGATGTTA ATGAGTGTCT GGATCCCACC ACCTGCATCA GTGGAAACTG TGTCAACACT CCCGGTAGTT ACACATGCGA TTGTCCTCCG GATTTTGAGC TGAATCCAAC TCGTGTCGGC TGTGTCGATA CTCGCTCTGG AAACTGCTAT CTGGATATCC GACCCCGGGG AGACAATGGA GATACAGCCT GCAGCAATGA AATTGGAGTT GGTGTCTCTA AGGCTTCCTG CTGTTGTTCA CTGGGTAAAG CTTGGGGAAC CCCATGTGAG CTGTGTCCTT CTGTGAACAC ATCTGAGTAT AAAATTCTTT GCCCTGGAGG AGAAGGTTTT CGTCCAAATC CCATCACCGT TATATTGGAA GACATCGATG AGTGCCAGGA GCTTCCAGGG CTGTGCCAAG GGGGGAAGTG CATCAATACC TTTGGCAGCT TCCAGTGTCG CTGTCCAACT GGTTACTACC TGAATGAAGA CACTCGAGTG TGTGATGATG TGAACGAATG TGAGACTCCT GGAATCTGTG GTCCGGGGAC CTGTTACAAC ACCGTTGGCA ACTATACCTG CATTTGTCCT CCAGACTACA TGCAAGTGAA CGGGGGAAAT AATTGCATGG ACATGAGAAG AAGTCTATGC TACAGAAACT ATTACGCTGA CAACCAGACC TGCGATGGAG AACTCCTGTT CAACATGACC AAGAAGATGT GCTGTTGCTC CTACAACATC GGCAGAGCCT GGAACAAACC CTGTGAACAG TGCCCCATCC CAAGCACAGA TGAGTTTGCT ACCCTCTGTG GGAGCCAGAG GCCCGGCTTC GTGATTGACA TTTATACGGG TTTACCCGTG GATATTGATG AATGCCGGGA GATCCCTGGG GTCTGTGAAA ATGGAGTGTG CATCAACATG GTTGGCAGCT TCCGGTGTGA GTGTCCCGTG GGATTCTTCT ATAACGACAA GTTACTGGTT TGTGAAGATA TCGACGAGTG TCAGAATGGC CCTGTGTGCC AGCGAAATGC GGAATGCATC AACACTGCAG GCAGCTACCG CTGTGACTGT AAGCCCGGCT ACCGCCTTAC CTCCACAGGT CAATGCAACG ATCGAAACGA GTGCCAAGAA ATCCCGAACA TATGCAGTCA TGGCCAGTGC ATCGACACCG TGGGAAGCTT CTACTGCCTT TGTCACACTG GCTTCAAAAC AAATGTGGAT CAGACCATGT GCTTAGACAT AAATGAGTGT GAGAGAGACG CCTGTGGGAA CGGGACTTGC AGAAACACGA TTGGCTCCTT CAACTGTCGC TGTAACCATG GCTTCATACT GTCTCACAAC AATGACTGCA TAGATGTTGA TGAGTGTGCA ACTGGAAACG GGAACCTTTG CAGAAATGGC CAGTGTGTCA ATACCGTGGG CTCCTTTCAG TGCAGGTGCA ATGAAGGCTA TGAGGTGGCT CCGGACGGCA GGACCTGTGT GGATATCAAC GAGTGTGTTC TGGATCCTGG GAAATGTGCA CCTGGAACCT GTCAGAACCT GGATGGCTCC TACAGATGCA TTTGCCCGCC TGGGTATAGT CTACAGAATG ACAAGTGTGA AGATATTGAT GAGTGTGTTG AAGAGCCAGA AATCTGTGCC TTGGGGACCT GCAGCAACAC TGAGGGTAGC TTCAAATGTC TGTGTCCAGA GGGGTTCTCC CTGTCCTCCA CTGGAAGAAG GTGCCAAGAT TTGCGAATGA GCTACTGCTA TGCGAAGTTT GAAGGTGGGA AGTGTTCATC ACCCAAATCC AGAAACCATT CCAAGCAGGA GTGCTGCTGT GCTTTGAAGG GAGAAGGCTG GGGAGATCCT TGTGAGTTGT GCCCCACTGA GCCAGATGAG GCTTTCCGCC AGATCTGCCC CTTTGGAAGT GGGATCATTG TGGGCCCTGA TGACTCAGCA GTTGATATGG ACGAATGCAA AGAACCTGAT GTCTGTAGAC ATGGGCAGTG CATTAACACA GACGGCTCCT ATCGATGCGA GTGTCCTTTT GGTTATATTC TGGAAGGGAA TGAGTGTGTG GATACCGATG AATGCTCTGT GGGCAATCCT TGTGGAAATG GGACCTGCAA GAATGTGATT GGAGGTTTTG AATGTACCTG TGAGGAGGGG TTCGAGCCTG GCCCAATGAT GACTTGTGAA GATATAAATG AATGTGCCCA GAATCCTCTG CTCTGCGCCT TCCGCTGTGT AAATACCTAC GGGTCCTATG AATGCAAATG CCCTGTTGGA TACGTTCTCC GAGAAGACAG GAGGATGTGT AAAGATGAGG ATGAGTGTGC AGAGGGAAAA CACGACTGTA CTGAGAAGCA AATGGAGTGT AAGAACCTCA TTGGTACCTA CATGTGCATC TGCGGCCCTG GGTACCAGCG CAGACCCGAT GGAGAGGGCT GCATAGATGA GAATGAGTGT CAGACCAAGC CCGGGATCTG TGAGAATGGG CGTTGCCTCA ACACCCTGGG TAGCTACACT TGTGAGTGTA ACGATGGCTT CACAGCCAGC CCCACTCAGG ATGAGTGCTT GGACAACCGG GAAGGGTACT GCTTTTCGGA GGTCTTGCAA AACATGTGCC AGATTGGCTC AAGCAACAGG AACCCCGTCA CCAAGTCCGA GTGCTGCTGT GATGGAGGGA GAGGCTGGGG ACCCCACTGT GAGATCTGCC CTTTCGAGGG CACAGTGGCT TACAAGAAGC TCTGTCCCCA CGGCCGAGGA TTCATGACCA ACGGAGCAGA TATTGATGAG TGCAAGGTTA TTCATGATGT TTGCCGAAAT GGGGAGTGTG TCAACGACAG AGGGTCCTAT CACTGCATCT GTAAAACTGG CTACACTCCG GATATAACAG GGACCGCCTG TGTAGATCTG AATGAATGCA ACCAGGCTCC CAAACCCTGC AATTTTATAT GCAAAAACAC AGAAGGGAGT TACCAGTGTT CCTGCCCGAA GGGCTACATT CTGCAAGAGG ATGGAAGGAG CTGCAAAGAT CTTGACGAGT GTGCAACCAA GCAGCATAAC TGTCAGTTCC TGTGTGTTAA CACCATCGGT GGCTTCACAT GCAAATGCCC TCCTGGGTTT ACCCAGCATC ACACTGCCTG CATTGATAAC AATGAGTGCA CGTCTGATAT CAACCTGTGT GGGTCCAAGG GTGTTTGCCA GAACACTCCA GGAAGCTTCA CCTGTGAATG CCAACGGGGG TTCTCACTCG ATCAGAGTGG TGCCAGCTGT GAAGATGTGG ACGAGTGTGA GGGTAACCAC CGCTGTCAAC ATGGCTGCCA GAACATCATC GGAGGCTATA GGTGTAGCTG CCCCCAGGGC TACCTCCAGC ACTACCAATG GAACCAGTGT GTAGATGAAA ACGAGTGCCT GAGTGCACAT GTCTGTGGAG GAGCCTCCTG CCACAACACC CTGGGGAGTT ACAAGTGCAT GTGTCCCACC GGCTTCCAGT ACGAACAGTT CAGTGGAGGC TGCCAAGACA TCAATGAGTG TGGCTCATCC CAGGCCCCCT GCAGTTACGG TTGCTCTAAT ACTGAGGGTG GCTACCTGTG TGGCTGTCCA CCAGGATACT TCCGGATAGG CCAAGGGCAT TGTGTTTCTG GAATGGGCAT GGGCCGAGGC GGCCCAGAGC CACCTGCCAG CAGCGAGATG GACGACAACT CACTGTCCCC AGAGGCCTGC TATGAGTGTA AGATCAACGG CTACCCAAAA CGAGGCCGGA AACGGAGAAG CACGAACGAA ACGGATGCCT CCGACATCCA GGACGGGTCT GAGATGGAAG CCAACGTGAG CCTCGCCAGC TGGGATGTGG AGAAGCCGGC TAGCTTTGCT TTCAATATTT CCCATGTCAA TAACAAGGTC CGAATCCTAG AGCTCCTGCC GGCCCTCACA ACTCTGATGA ACCACAACAG ATACTTGATT GAATCTGGAA ATGAAGATGG CTTCTTTAAA ATCAACCAGA AAGAAGGGGT CAGCTACCTC CACTTCACGA AGAAGAAGCC GGTGGCTGGG ACCTACTCCT TACAAATCAG CAGCACCCCA CTTTATAAAA AGAAAGAACT TAACCAGTTA GAAGACAGAT ATGACAAAGA CTACCTCAGT GGTGAACTGG GCGATAACCT GAAGATGAAA ATTCAGATCT TGCTGCATCT CGAGTACCCA TACGACGTAC CTGATTATGC AACTTATCCT TATGATGTAC CTGATTATGC TAGCTATCCT TATGATGTCC CAGACTACGC ATAGGGATCC CTGCGATATC CAGCGCGGCC GCGAAGGATC TGCGATCGCT CCGGTGCCCG TCAGTGGGCA GAGCGCACAT CGCCCACAGT CCCCGAGAAG TTGGGGGGAG GGGTCGGCAA TTGAACGGGT GCCTAGAGAA GGTGGCGCGG GGTAAACTGG GAAAGTGATG TCGTGTACTG GCTCCGCCTT TTTCCCGAGG GTGGGGGAGA ACCGTATATA AGTGCAGTAG TCGCCGTGAA CGTTCTTTTT CGCAACGGGT TTGCCGCCAG AACACAGCTG AAGCTTCGAG GGGCTCGCAT CTCTCCTTCA CGCGCCCGCC GCCCTACCTG AGGCCGCCAT CCACGCCGGT TGAGTCGCGT TCTGCCGCCT CCCGCCTGTG GTGCCTCCTG AACTGCGTCC GCCGTCTAGG TAAGTTTAAA GCTCAGGTCG AGACCGGGCC TTTGTCCGGC GCTCCCTTGG AGCCTACCTA GACTCAGCCG GCTCTCCACG CTTTGCCTGA CCCTGCTTGC TCAACTCTAC GTCTTTGTTT CGTTTTCTGT TCTGCGCCGT TACAGATCCA AGCTGTGACC GGCGCCTACG CTAGACGCCA CCATGGAGAG CGACGAGAGC GGCCTGCCCG CCATGGAGAT CGAGTGCCGC ATCACCGGCA CCCTGAACGG CGTGGAGTTC GAGCTGGTGG GCGGCGGAGA GGGCACCCCC AAGCAGGGCC GCATGACCAA CAAGATGAAG AGCACCAAAG GCGCCCTGAC CTTCAGCCCC TACCTGCTGA GCCACGTGAT GGGCTACGGC TTCTACCACT TCGGCACCTA CCCCAGCGGC TACGAGAACC CCTTCCTGCA CGCCATCAAC AACGGCGGCT ACACCAACAC CCGCATCGAG AAGTACGAGG ACGGCGGCGT GCTGCACGTG AGCTTCAGCT ACCGCTACGA GGCCGGCCGC GTGATCGGCG ACTTCAAGGT GGTGGGCACC GGCTTCCCCG AGGACAGCGT GATCTTCACC GACAAGATCA TCCGCAGCAA CGCCACCGTG GAGCACCTGC ACCCCATGGG CGATAACGTG CTGGTGGGCA GCTTCGCCCG CACCTTCAGC CTGCGCGACG GCGGCTACTA CAGCTTCGTG GTGGACAGCC ACATGCACTT CAAGAGCGCC ATCCACCCCA GCATCCTGCA GAACGGGGGC CCCATGTTCG CCTTCCGCCG CGTGGAGGAG CTGCACAGCA ACACCGAGCT GGGCATCGTG GAGTACCAGC ACGCCTTCAA GACCCCCATC GCCTTCGCCA GATCCCGCGC TCAGTCGTCC AATTCTGCCG TGGACGGCAC CGCCGGACCC GGCTCCACCG GATCTCGCGA GGGCAGAGGA AGTCTTCTAA CATGCGGTGA CGTGGAGGAG AATCCCGGCC CTATGACCGA GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GAAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAGCT GACGTCCTTT CCATGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TCCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGCC GCCTCCCCGC CTGGTACCTT TAAGACCAAT GACTTACAAG GCAGCTGTAG ATCTTAGCCA CTTTTTAAAA GAAAAGGGGG GACTGGAAGG GCTAATTCAC TCCCAACGAA AATAAGATCT GCTTTTTGCT TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGTA GTAGTTCATG TCATCTTATT ATTCAGTATT TATAACTTGC AAAGAAATGA ATATCAGAGA GTGAGAGGAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG CTCTAGCTAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGACT TTTGCAGAGA CGGCCCAAAT TCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGC CAAGCTG
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