NM_009781.4;Cav1.2; Cchl1a1; D930026N18Rik; MBC;MELC-CC
100μg liquid plasmid(Instantly):$163
| name | pLV3-CMV-Cacna1c(mouse)-3×FLAG-Puro | another name | apLV3-CMV-Cacna1c(mouse)-3×FLAG-Puro |
| Insert Size (bp) | 6507bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG TGACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT GACCTCCATA GAAGATTCTA GAGCTAGCGA ATTCGCCACC ATGATTCGGG CCTTTGTTCA GCCATCCACA CCCCCATACC AGCCACTCTC CAGTCACTCG TCTGAGGAAA CAGAGAGGAA GTTCAAGGGC AAGGTGGTAC ATGAAGCTCA ACTCAACTGT TTCTACATTT CTCCTGGAGG TTCCAACTAT GGGAGCCCAC GCCCAGCTCA TGCCAACATG AATGCCAATG CAGCTGCAGG ACTTGCTCCC GAGCACATCC CTACTCCAGG GGCAGCACTG TCCTGGCAGG CAGCCATCGA TGCCGCCCGG CAGGCCAAGC TCATGGGCAG TGCTGGCAAC GCAACCATCT CTACCGTCAG TTCCACACAG CGGAAGCGGC AGCAGTATGG GAAACCCAAG AAGCAGGGGG GCACAACCGC CACACGGCCG CCCCGGGCTC TGCTGTGTCT GACCCTGAAG AACCCTATCC GGAGGGCGTG CATAAGCATT GTTGAATGGA AACCATTTGA AATCATTATT TTACTGACTA TTTTTGCCAA TTGTGTGGCC TTAGCAATCT ATATTCCCTT TCCGGAAGAC GACTCCAACG CCACCAACTC CAACCTGGAA CGAGTGGAGT ATCTCTTCCT CATCATTTTT ACCGTGGAAG CATTTTTAAA AGTAATTGCC TACGGACTTC TCTTCCACCC CAACGCTTAC CTCCGCAATG GTTGGAATTT ACTGGATTTT ATAATCGTGG TTGTAGGGCT TTTTAGTGCA ATTTTAGAAC AAGCAACCAA AGCTGATGGG GCCAATGCTC TAGGAGGGAA AGGAGCTGGA TTCGACGTGA AGGCACTGAG AGCGTTCCGC GTGCTCCGTC CACTGCGGCT AGTGTCCGGA GTCCCAAGTC TCCAGGTGGT CCTGAACTCC ATCATCAAGG CCATGGTGCC TCTGCTGCAC ATTGCCCTTC TTGTGCTCTT CGTCATCATC ATTTATGCTA TTATCGGCCT GGAGCTCTTC ATGGGAAAGA TGCACAAGAC CTGCTACAAC CAGGAGGGCA TAATAGATGT TCCGGCAGAA GAGGATCCTT CCCCTTGTGC TTTGGAGACA GGCCATGGGC GACAGTGTCA GAACGGGACC GTGTGCAAAC CCGGGTGGGA TGGGCCCAAG CACGGCATCA CCAACTTCGA CAACTTCGCC TTCGCCATGC TGACGGTGTT CCAGTGTATC ACCATGGAGG GCTGGACAGA CGTGCTGTAC TGGATGCAAG ACGCTATGGG CTATGAGTTG CCCTGGGTGT ATTTTGTCAG TCTGGTCATC TTTGGATCCT TTTTCGTTCT AAATCTGGTT CTCGGTGTTT TGAGCGGGGA GTTTTCCAAA GAGAGGGAGA AAGCCAAAGC CCGAGGAGAT TTCCAGAAGC TTCGAGAGAA GCAGCAACTA GAAGAAGATC TCAAAGGCTA CCTGGACTGG ATCACCCAGG CAGAAGACAT TGACCCCGAG AATGAGGACG AGGGCATGGA TGAAGACAAG CCTCGAAACA TGAGCATGCC CACAAGTGAG ACTGAGTCTG TCAACACTGA AAACGTGGCT GGAGGTGACA TCGAGGGAGA AAACTGTGGA GCCAGGCTTG CCCATCGGAT CTCCAAATCC AAATTCAGCC GCTACTGGCG CAGGTGGAAT CGATTCTGCA GAAGAAAATG CCGTGCAGCA GTTAAGTCCA ACGTCTTCTA CTGGCTCGTG ATCTTCCTGG TGTTCCTCAA CACCCTCACC ATTGCCTCCG AACATTACAA CCAGCCTCAC TGGCTCACAG AAGTGCAAGA CACAGCCAAT AAAGCCCTCC TGGCCCTTTT CACTGCAGAA ATGCTCCTGA AGATGTACAG CCTGGGTCTT CAGGCCTATT TTGTGTCCCT CTTCAACCGC TTTGACTGTT TCATTGTGTG TGGGGGCATC CTGGAGACCA TCCTGGTGGA GACGAAGATC ATGTCTCCCC TGGGCATCTC TGTGCTGAGA TGTGTGCGGT TGCTCAGGAT CTTCAAGATC ACCAGGTACT GGAATTCCTT GAGCAACCTT GTGGCATCCT TGCTGAACTC AGTGCGCTCC ATTGCCTCCC TGCTGCTGCT CCTCTTCCTC TTCATCATCA TCTTCTCCCT CCTGGGGATG CAGCTCTTTG GAGGGAAGTT CAATTTCGAT GAGATGCAGA CCCGTAGGAG CACGTTCGAT AACTTCCCGC AGTCTCTCCT CACTGTGTTT CAGATCCTGA CCGGGGAGGA CTGGAATTCG GTGATGTATG ATGGGATCAT GGCTTATGGC GGCCCCTCTT TTCCAGGGAT GTTAGTCTGT ATTTACTTCA TCATCCTCTT CATCTGTGGA AATTATATCC TACTGAATGT GTTCTTGGCC ATTGCGGTGG ACAACCTGGC TGATGCGGAG AGCCTGACCT CAGCCCAAAA GGAGGAGGAA GAAGAGAAGG AGAGGAAGAA GCTGGCCAGG ACTGCCAGCC CAGAAAAGAA ACAGGAGGTG ATGGAGAAGC CAGCCGTGGA GGAGAGCAAA GAGGAGAAAA TTGAACTGAA ATCCATTACA GCCGATGGAG AATCCCCACC CACTACCAAG ATCAACATGG ATGACCTCCA GCCCAGTGAA AACGAGGATA AGAGTCCCCA CTCCAACCCA GACACTGCAG GGGAAGAGGA TGAAGAGGAG CCAGAGATGC CTGTGGGGCC ACGCCCCCGG CCCCTGTCTG AGCTGCACCT TAAGGAAAAG GCAGTTCCCA TGCCGGAAGC CAGTGCATTT TTCATCTTCA GCCCAAACAA CAGGTTCCGC CTGCAGTGCC ACCGTATTGT CAATGACACG ATCTTCACCA ACCTCATCCT CTTCTTCATT CTGCTCAGCA GCATCTCTCT GGCTGCTGAG GACCCCGTCC AGCACACCTC CTTCAGGAAC CATATCCTAG GCAATGCAGA CTATGTCTTC ACTAGTATCT TTACATTAGA AATTATCCTT AAGATGACTG CTTACGGGGC TTTCCTGCAC AAGGGCTCTT TCTGCCGAAA CTACTTCAAT ATCCTGGACC TGCTGGTGGT TAGCGTGTCC CTCATCTCCT TTGGCATCCA GTCCAGCGCG ATCAACGTTG TGAAGATTTT ACGAGTGCTG CGAGTCCTCA GACCCCTGAG GGCCATCAAC AGGGCCAAGG GGCTAAAGCA TGTGGTTCAG TGCGTGTTTG TGGCCATCCG GACCATCGGG AACATCGTAA TTGTCACCAC TCTGCTGCAG TTCATGTTCG CCTGCATTGG GGTCCAGCTC TTCAAGGGAA AGCTCTATAC CTGTTCGGAT AGTTCTAAAC AGACGGAGGC AGAATGCAAG GGTAACTATA TCACATACAA AGATGGAGAG GTCGATCACC CCATTATCCA GCCTCGAAGC TGGGAGAACA GCAAGTTTGA CTTTGACAAT GTTTTGGCAG CCATGATGGC TCTCTTCACC GTCTCCACCT TCGAAGGGTG GCCAGAGCTG CTGTACCGCT CCATTGACTC CCACACAGAA GACAAGGGCC CCATCTACAA CTACCGTGTG GAGATCTCCA TCTTCTTCAT CATCTATATC ATCATCATTG CCTTCTTCAT GATGAACATC TTCGTGGGTT TCGTCATTGT CACCTTCCAG GAGCAGGGGG AACAAGAGTA CAAGAACTGT GAGCTGGACA AGAACCAGAG ACAATGTGTG GAATATGCCC TCAAGGCCCG ACCCTTGCGA AGGTACATCC CCAAGAACCA GCACCAGTAC AAAGTGTGGT ACGTGGTCAA CTCTACCTAC TTCGAGTATC TGATGTTCGT TCTCATCCTG CTCAACACCA TCTGCCTGGC CATGCAGCAC TATGGCCAGA GCTGCCTCTT CAAAATCGCC ATGAATATAC TCAACATGCT TTTCACCGGC CTCTTCACAG TGGAGATGAT CCTGAAGCTC ATTGCCTTCA AACCCAAGGG TTACTTTAGT GATCCCTGGA ATGTTTTTGA CTTCCTCATC GTCATTGGGA GCATAATTGA TGTCATTCTC AGTGAGACTA ATCCAGCTGA ACATACCCAA TGCTCTCCCT CTATGAGTGC AGAGGAGAAC TCCCGCATCT CCATCACCTT CTTCCGCCTC TTCCGGGTCA TGCGCCTGGT GAAGCTGCTG AGCCGCGGGG AAGGCATCCG AACCCTGCTG TGGACCTTCA TCAAGTCCTT CCAGGCTCTG CCCTATGTGG CTCTTTTGAT TGTGATGCTG TTCTTTATCT ATGCAGTGAT TGGGATGCAG GTGTTTGGGA AGATTGCCCT GAATGACACC ACAGAGATCA ATCGGAACAA CAACTTCCAG ACGTTCCCCC AGGCTGTGTT ACTGCTGTTC AGGTGTGCCA CCGGAGAGGC CTGGCAGGAC ATCATGCTGG CCTGCATGCC AGGCAAGAAG TGTGCCCCAG AGTCTGAGCC CAGCAACAGC ACGGAAGGGG AGACCCCCTG TGGCAGCAGC TTTGCTGTCT TCTACTTCAT CAGCTTCTAC ATGCTCTGTG CCTTCCTGAT CATCAACCTC TTTGTAGCTG TTATCATGGA CAACTTTGAC TACCTGACTA GGGATTGGTC TATCCTCGGT CCCCATCACC TGGATGAATT CAAGAGAATC TGGGCCGAGT ATGACCCTGA AGCCAAGGGT CGGATCAAAC ACTTGGATGT GGTGACCCTC CTCCGTCGAA TTCAGCCCCC ACTGGGTTTT GGGAAATTGT GTCCTCACCG TGTGGCCTGC AAACGCCTGG TGTCCATGAA CATGCCTCTG AACAGCGATG GCACAGTCAT GTTCAATGCT ACCCTGTTTG CCCTCGTCAG GACAGCCCTG AGGATCAAAA CAGAAGGGAA CCTAGAGCAA GCCAATGAGG AGCTTCGGGC CATCATCAAG AAAATCTGGA AGAGGACTAG CATGAAGCTG TTGGACCAGG TGGTGCCCCC TGCAGGCGAT GACGAGGTCA CAGTGGGCAA GTTCTATGCC ACCTTCCTGA TCCAAGAGTA CTTCAGGAAA TTCAAGAAGC GAAAAGAGCA GGGGCTGGTG GGCAAGCCCT CACAAAGGAA TGCACTGTCC CTCCAGGCTG GCTTGCGCAC CTTGCATGAC ATTGGGCCTG AGATCCGGCG GGCCATCTCT GGGGATCTGA CTGCTGAGGA GGAGTTGGAC AAGGCTATGA AGGAGGCGGT GTCTGCTGCC TCCGAAGATG ACATCTTCAG GAGGGCTGGA GGCCTGTTCG GCAACCATGT CACCTACTAT CAGAGTGACA GCAGGGGCAA CTTTCCTCAG ACGTTCGCCA CCCAGCGCCC ACTGCACATC AACAAGACAG GGAACAACCA AGCTGACACT GAGTCACCGT CCCATGAGAA GCTGGTGGAC TCCACGTTCA CCCCCAGCAG CTACTCATCC ACGGGCTCCA ATGCCAACAT CAACAATGCC AACAACACTG CCCTGGGCCG CTTCCCCCAT CCCGCTGGCT ACTCCAGCAC GGTCAGCACT GTGGAGGGCC ATGGGCCTCC CTTGTCCCCT GCTGTCCGAG TACAGGAGGC AGCATGGAAG CTCAGCTCTA AGAGGTGCCA CTCCCGAGAG AGCCAGGGAG CCACGGTGAA TCAGGAGATA TTTCCAGATG AGACCCGCAG CGTAAGGATG AGTGAAGAAG CCGAGTACTG CAGTGAGCCC AGCCTGCTCT CCACAGATAT GTTCTCCTAC CAGGAAGATG AACACCGACA ACTGACCTGC CCAGAGGAGG ACAAGAGGGA GATCCAGCCA TCTCCAAAGA GGAGTTTCCT TCGCTCTGCC TCTCTAGGTC GAAGGGCCTC CTTCCATCTG GAATGTCTAA AGCGACAAAA GGATCAAGGG GGAGACATCT CTCAGAAGAC AGCCTTGCCC TTGCATCTGG TTCATCATCA GGCATTGGCA GTGGCAGGCT TGAGCCCCCT CCTGCAGAGA AGCCATTCTC CTACCACATT CCCCAGGCCG TGCCCCACAC CCCCTGTCAC TCCAGGCAGC CGGGGCAGAC CCCTACGGCC CATCCCTACC CTACGGCTGG AGGGGGCAGA GTCCAGCGAG AAACTCAACA GCAGCTTCCC ATCCATCCAC TGCAGCTCCT GGTCTGAGGA GACGACAGCC TGTAGTGGGA GCAGCAGCAT GGCCCGGAGA GCCCGGCCCG TCTCCCTCAC CGTGCCCAGC CAGGCTGGAG CTCCAGGGAG ACAGTTCCAT GGCAGTGCCA GCAGCCTGGT GGAAGCGGTC TTGATTTCAG AAGGACTGGG ACAGTTTGCT CAAGATCCCA AGTTCATCGA GGTCACCACC CAGGAGCTGG CTGACGCCTG CGACATGACA ATAGAGGAGA TGGAGAACGC CGCAGACAAC ATCCTCAGTG GGGGCGCCCA GCAGAGCCCC AACGGCACCC TCTTACCTTT TGTGAACTGC AGGGACCCGG GGCAGGACAG GGCTGTGGCC CCAGAGGACG AGAGCTGCGC ATATGCCCTG GGGCGAGGCC GGAGCGAGGA GGCGCTCGCG GACAGCAGGT CCTACGTCAG CAACCTGGGA TCCGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG ACATCGACTA CAAGGATGAC GATGACAAGT AGCGGCCGCG AAGGATCTGC GATCGCTCCG GTGCCCGTCA GTGGGCAGAG CGCACATCGC CCACAGTCCC CGAGAAGTTG GGGGGAGGGG TCGGCAATTG AACGGGTGCC TAGAGAAGGT GGCGCGGGGT AAACTGGGAA AGTGATGTCG TGTACTGGCT CCGCCTTTTT CCCGAGGGTG GGGGAGAACC GTATATAAGT GCAGTAGTCG CCGTGAACGT TCTTTTTCGC AACGGGTTTG CCGCCAGAAC ACAGCTGAAG CTTCGAGGGG CTCGCATCTC TCCTTCACGC GCCCGCCGCC CTACCTGAGG CCGCCATCCA CGCCGGTTGA GTCGCGTTCT GCCGCCTCCC GCCTGTGGTG CCTCCTGAAC TGCGTCCGCC GTCTAGGTAA GTTTAAAGCT CAGGTCGAGA CCGGGCCTTT GTCCGGCGCT CCCTTGGAGC CTACCTAGAC TCAGCCGGCT CTCCACGCTT TGCCTGACCC TGCTTGCTCA ACTCTACGTC TTTGTTTCGT TTTCTGTTCT GCGCCGTTAC AGATCCAAGC TGTGACCGGC GCCTACGCTA GATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC CCCGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG TCGACCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC CCGGTGCCTG AGTCGACAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAATCATCGT CCTTTCCTTG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCCTGG TACCTTTAAG ACCAATGACT TACAAGGCAG CTGTAGATCT TAGCCACTTT TTAAAAGAAA AGGGGGGACT GGAAGGGCTA ATTCACTCCC AACGAAAATA AGATCTGCTT TTTGCTTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA GTCAGTGTGG AAAATCTCTA GCAGTAGTAG TTCATGTCAT CTTATTATTC AGTATTTATA ACTTGCAAAG AAATGAATAT CAGAGAGTGA GAGGAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGCTCT AGCTATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGACTTTTG CAGAGACGGC CCAAATTCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGCCAAG CTG
