pTol2-ZBactin-AFPI(winter flounder)-EGFP
$84.00
In stock
SKU
PL36818
EU188795.1
100μg liquid plasmid(Instantly):$105
| name | pTol2-ZBactin-AFPI(winter flounder)-EGFP | another name | apTol2-ZBactin-AFPI(winter flounder)-EGFP |
| Insert Size (bp) | 654bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression,Transpose | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Neo/G418 | Fluorescent labeling | Green |
| Promoter | ZBactin | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAGTTATTAA TAGAGGTGTA AAGTACTTGA GTAATTTTAC TTGATTACTG TACTTAAGTA TTATTTTTGG GGATTTTTAC TTTACTTGAG TACAATTAAA AATCAATACT TTTACTTTTA CTTAATTACA TTTTTTTAGA AAAAAAAGTA CTTTTTACTC CTTACAATTT TATTTACAGT CAAAAAGTAC TTATTTTTTG GAGATCACTT CATTCTATTT TCCCTTGCTA TTAGCTAGCG CTACCGGACT CAGATCTCGA GCGTTTACTC TGAGAAACTT CCATATTTTA GAGGTAAAAT GCTCAGAATT GTAAAAAGTC TTGTCAAAAT ACTTGGAATT TGCCAAAAAC AAAATAAAAG TCACATCACC TCATTGTCCT TGGTATCAGC AAGCACTTTT AACACAATCA GCTGTTTTTG TTTATAACTC CCACTGAAGG CCATGTGCAT TACTCCCAAG TTACACAGTA AAAAGTCACA TGTAGTTTAT CATGATCACT GCTGCTCAAG CACAGCTTTA TCTGTTATTC AGTGTGGCAA TATCTGAAAT GTGTGCAAAA TATTATTTAT TGTAGCTGAT CACCAACACT TTCAGTGTTT TTTTTTTCTA CTTTTAATGA GAATTATGTT CTTAGGATTT ATCCTTATAA ACCTCTTTAA AAAGAAATGT TATGTCTGCA TAGACACGAC ATGAAATTAT GAACAGAAAA TTTAACTATT TAAGATCAAG ACTTGACATA TAACATTTCT AATGAACATT AAACCTGAGG TAGGCCCACT TGCAAAAATG TAACTGCTAT CACTCACTTT ATACATCATT GTGTAACTTG TACACACAAA AATAGTGAAG CAATGTGGCA TGGAATGCAG GTCCCATTCA TTCAAAGTTA TTAAAGCACT TTCTTAAAGT GCATTACTAC TGAGCTAAGA CCAGCTAGTT GCTCATACAA CATACTACAA GTAGTGTCAT CCTAATTTGG ATAAAAAAAA TGTCCATTTT ATACATGGAA AAGTACTCAT TAGATCCCCC ATTGTTTGTA TTACGGTATT TCGTGAACAC AAGAGGTAAA TGACCTACAG AGCTGCTGTT GTGTTAGATT GAAAACACAA CACAGGATCA TGGAGGCATC CATCATTATG ACGCATTCTG CCACTTGTAA CTCTGCACAG ACTTTTTGAA AAGTTTAATT GAGTCTTGGC GTTGTCGCAG AAAAATACGA AAAATTCTGT GTTAAAATTT GCAAATAAAA ATTCCAGTTT TATAGGAAAT CGCCATGGCC TTGTTCTTTA ACCAGTTAAG CTTCCCCTTC TTTCACTCTC AAGTTGCAAG AAGCAAGTGT AGCAATGTGC ACGCGACAGC CGGGTGTGTG ACGCTGGACC AATCAGAGCA CAGAGCTCCG AAAGTTTACC TTTTATGGCT AGAGCCGGGT ATGTGCCGTC ATATAAAAGA GCGCGCCCAG CTTTTCAGCC TCACTTTGAG CTCCTCCACA CGCAGCTAGT GCGGAATATC ATCTGCTTGT AACCCATTCT CTTAAGTCGA CAACCCCCCA AACCCAAGGT GAGTTGGTTT TACAGTTTTT AAGCGTTTAA TTATAGTTTC TATCTATTAA TGTTAAGTAA AATGTGTAAA TGGATAAATA TGATTTGATG AAGTCTTTAG ACAGTTCATA ACGGTCTCTT TGTAGTTATC TACTAGGTTT GATTTATAAG TGGTGGTTCT TTTGATAATC TATGTTACAT TTAATAAATA TTGGCTGTAT CTTAACTTTT TTGTAGCGTT AGTGGATGTG GCAGGTGAGA ATGTCGGTAA TTTAACGTGA CCAGTCGTAG GCACGACATT GAACATTGAA TAGGCCGGTG TGAAATGGGT GTTAAGTCTT AATATAAAGT TGTGTTCTGG CTAATCTCTC TTTAACAGCC ATAAATGTGA TTTTTAACTA CAGTTTTGAG GGTGCTGGTG AGCATGTTGC ACACTTGATG GATAGCCGGC ATGGGAAGTT CTTTGTGCAG GCAGTGCTGC AGCAGGGTGT GACCTACTTT AGCTAGCCGG CTAACCAGCT CTCATCTGAT GTAACGTAAA CCCCATCAAT CTAACATCGG CTCATTCGTG CTTTATAAGA TCGAAGATCT TGTTATTAGT AACGGGTATT GGCATTTAGT TTTAACTTGG TTAATTTTCT TTTTGTTATG TGTTCTATTT AAATGTAGCC TGCAGCCCCC TCTGTCTTTA ATGAAGCTGA AGATTAACGT TAACGTTAGT GAAGTGTTGT ATCCGCTATG AAAGATTTGA ATTTTAGAGG TGTGGTCCAC TTTAATAACG GCTGCGGAGT TCTCTGGCTG CACTTCAGCC GCTGTGACTG TCGAAGCAGA GCCCATCAGT TTTTCAGGAT CTCGGTGATT TTTGAGGCTA GACTATGAAC TGAACCGACT GAGAGTGACC GTCCAGAAAT GCTTTAGTGT GAAGTCTCCG CTCACGAGGA GTCCGTGGAC TGACTGCAGA TCTGTGACGC AGTAACACCC GGCGGCAGAC ACCCGTTGAA ATGCGGTTGT GTAATTGATC GCAGGCGAGG GTGGAAGAGG ATGTGAAACT TCATTTGTGT AAAATTTAGG GAGTGGTCCT GGTGTGATGA ATGTCGAAAT CTGTTCCTTT TTACTGAGCC CTACGACTCT GGCTGAGTGC CACACCGCCG GCAGCCGCAA AGCGGCTCAA ACCATTGCCT TTTATGGTAA TAATGAGAGA ATGCAGAGGG ACTTCCTTTG TCTGGCATAT CTGAGGCGCG CGCTGTCACT AGCGCCCACC AGCGGTCAGA CTGTAGAATG CAGAGCAAAA CAGGAAGTTG ACTCCAGATG GTCACATGCT TGCTGAAACG CCTCTCCCTG GCAGCAGTGC AATTCTAACA GTTTCCCTTT CTTTTACAGT TCAGCCGAAT TCTGCAGTCG ACGGTACCAG CCACCATGGC TCTCTCACTT TTCACTGTCG GACAATTCAT TTTCTTATTT TGGACAATCA GCATCACTGA AGCCAACATC GACCCCGCAG CCAGAGCCGC AGCAGCCGCA GCAGCCTCCA AAGCCGCAGT CACCGCCGCC GACGCTGCCG CAGCCGCTGC CACCATCGCC GCATCCGCCG CCTCAGTCGC TGCGGCCACT GCTGCTGATG ACGCCGCCGC ATCCATAGCA ACCATCAACG CCGCATCCGC TGCCGCGAAA TCCATCGCCG CCGCCGCAGC AATGGCAGCC AAGGACACTG CCGCCGCCGC GGCCAGCGCC GCCGCCGCCG CCGTTGCCTC CGCCGCCAAA GCCCTAGAAA CCATCAACGT CAAAGCCGCA TACGCCGCTG CCACCACCGC TAATACCGCT GCTGCCGCCG CCGCCGCCAC CGCCACCACC GCCGCTGCCG CCGCCGCAGC CAAAGCAACC ATCGACAACG CCGCCGCTGC CAAGGCAGCC GCAGTCGCCA CCGCCGTTTC AGATGCCGCA GCCACCGCCG CCACTGCCGC CGCCGTTGCC GCTGCAACCC TCGAAGCTGC CGCCGCAAAA GCCGCAGCCA CTGCAGTCTC TGCCGCCGCC GCTGCTGCCG CCGCCGCCAT CGCCTTCGCC GCTGCCCCAG GATCCGGAGG TGGCAGCGGT GGCGGTCAGC TGTTGAATTT TGACCTTCTT AAACTTGCGG GAGACGTCGA GTCCAACCCT GGGCCCACAA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT AAAGCGGCCG CGACTCTAGA TCATAATCAG CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA CCTGAAACAT AAAATGAATG CAATTGTTGT TGTTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTAAGGC TTAAACAAGA ATCTCTAGTT TTCTTTCTTG CTTTTACTTT TACTTCCTTA ATACTCAAGT ACAATTTTAA TGGAGTACTT TTTTACTTTT ACTCAAGTAA GATTCTAGCC AGATACTTTT ACTTTTAATT GAGTAAAATT TTCCCTAAGT ACTTGTACTT TCACTTGAGT AAAATTTTTG AGTACTTTTT ACACCTCTGC TTAAGGCGTA AATTGTAAGC GTTAATATTT TGTTAAAATT CGCGTTAAAT TTTTGTTAAA TCAGCTCATT TTTTAACCAA TAGGCCGAAA TCGGCAAAAT CCCTTATAAA TCAAAAGAAT AGACCGAGAT AGGGTTGAGT GTTGTTCCAG TTTGGAACAA GAGTCCACTA TTAAAGAACG TGGACTCCAA CGTCAAAGGG CGAAAAACCG TCTATCAGGG CGATGGCCCA CTACGTGAAC CATCACCCTA ATCAAGTTTT TTGGGGTCGA GGTGCCGTAA AGCACTAAAT CGGAACCCTA AAGGGAGCCC CCGATTTAGA GCTTGACGGG GAAAGCCGGC GAACGTGGCG AGAAAGGAAG GGAAGAAAGC GAAAGGAGCG GGCGCTAGGG CGCTGGCAAG TGTAGCGGTC ACGCTGCGCG TAACCACCAC ACCCGCCGCG CTTAATGCGC CGCTACAGGG CGCGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTCCTG AGGCGGAAAG AACCAGCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAGATCG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCTAGG GGGAGGCTAA CTGAAACACG GAAGGAGACA ATACCGGAAG GAACCCGCGC TATGACGGCA ATAAAAAGAC AGAATAAAAC GCACGGTGTT GGGTCGTTTG TTCATAAACG CGGGGTTCGG TCCCAGGGCT GGCACTCTGT CGATACCCCA CCGAGACCCC ATTGGGGCCA ATACGCCCGC GTTTCTTCCT TTTCCCCACC CCACCCCCCA AGTTCGGGTG AAGGCCCAGG GCTCGCAGCC AACGTCGGGG CGGCAGGCCC TGCCATAGCC TCAGGTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCAT GCAT
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