XP613-CBC-Psenen(mouse)-Neo
$70.00
In stock
SKU
PL36782
NM_025498.3;1700023M09Rik; Pen-2; Pen2
100μg liquid plasmid(Instantly):$91
| name | XP613-CBC-Psenen(mouse)-Neo | another name | aXP613-CBC-Psenen(mouse)-Neo |
| Insert Size (bp) | 306bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CBC | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTTG GTACCCCTGT CAGTTAACGG CAGCCGGAGT GCGCAGCCGC CGGCAGCCTC GCTCTGCCCA CTGGGTGGGG CGGGAGGTAG GTGGGGTGAG GCGAGCTGGA CGTGCGGGCG CGGTCGGCCT CTGGCGGGGC GGGGGAGGGG AGGGAGGGTC AGCGAAAGTA GCTCGCGCGC GAGCGGCCGC CCACCCTCCC CTTCCTCTGG GGGAGTCGTT TTACCCGCCG CCGGCCGGGC CTCGTCGTCT GATTGGCTCT CGGGGCCCAG AAAACTGGCC CTTGCCATTG GCTCGTGTTC GTGCAAGTTG AGTCCATCCG CCGGCCAGCG GGGGCGGCGA GGAGGCGCTC CCAGGTTCCG GCCCTCCCCT CGGCTCCGCG CCGCAGAGTC TGGCCGCGCG CCCCTGCGCA ACGTGGCAGG AAGCGCGCGC TGGGGGCGGG GACGGGCAGT AGGGCTGAGC GGCTGCGGGG CGGGTGCAAG CACGTTTCCG ACTTGAGTTG CCTCAAGAGG GGCGTGCTGA GCCAGACCTC CATCGCGCAC TCCGGGGAGT GGAGGGAAGG AGCGAGGGCT CAGTTGGGCT GTTTTGGAGG CAGGAAGCAC TTGCTCTCCC AAAGTCGCTC TGAGTTGTTA TCAGTAAGGG AGCTGCAGTG GAGTAGGCGG GGAGAAGGCC GCACCCTTCT CCGGAGGGGG GAGGGGAGTG TTGCAATACC TTTCTGGGAG TTCTCTGCTG CCTCCTGGCT TCTGAGGACC GCCCTGGGCC TGGGAGAATC CCTTCCCCCT CTTCCCTCGT GATCTGCAAC TCCAGTCTTT CTAGAATCGA TGGCGCGCCG GATTCGACAT TGATTATTGA CTAGTTATTA ATAGTAATCA ATTACGGGGT CATTAGTTCA TAGCCCATAT ATGGAGTTCC GCGTTACATA ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC CCCCGCCCAT TGACGTCAAT AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA GTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT TATGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTCGA GGTGAGCCCC ACGTTCTGCT TCACTCTCCC CATCTCCCCC CCCTCCCCAC CCCCAATTTT GTATTTATTT ATTTTTTAAT TATTTTGTGC AGCGATGGGG GCGGGGGGGG GGGGGGGCGC GCGCCAGGCG GGGCGGGGCG GGGCGAGGGG CGGGGCGGGG CGAGGCGGAG AGGTGCGGCG GCAGCCAATC AGAGCGGCGC GCTCCGAAAG TTTCCTTTTA TGGCGAGGCG GCGGCGGCGG CGGCCCTATA AAAAGCGAAG CGCGCGGCGG GCGGGAGTCG CTGCGCGCTG CCTTCGCCCC GTGCCCCGCT CCGCCGCCGC CTCGCGCCGC CCGCCCCGGC TCTGACTGAC CGCGTTACTC CCACAGGTGA GCGGGCGGGA CGGCCCTTCT CCTCCGGGCT GTAATTAGCG CTTGGTTTAA TGACGGCTTG TTTCTTTTCT GTGGCTGCGT GAAAGCCTTG AGGGGCTCCG GGAGGGCCCT TTGTGCGGGG GGAGCGGCTC GGGGGGTGCG TGCGTGTGTG TGTGCGTGGG GAGCGCCGCG TGCGGCTCCG CGCTGCCCGG CGGCTGTGAG CGCTGCGGGC GCGGCGCGGG GCTTTGTGCG CTCCGCAGTG TGCGCGAGGG GAGCGCGGCC GGGGGCGGTG CCCCGCGGTG CGGGGGGGGC TGCGAGGGGA ACAAAGGCTG CGTGCGGGGT GTGTGCGTGG GGGGGTGAGC AGGGGGTGTG GGCGCGTCGG TCGGGCTGCA ACCCCCCCTG CACCCCCCTC CCCGAGTTGC TGAGCACGGC CCGGCTTCGG GTGCGGGGCT CCGTACGGGG CGTGGCGCGG GGCTCGCCGT GCCGGGCGGG GGGTGGCGGC AGGTGGGGGT GCCGGGCGGG GCGGGGCCGC CTCGGGCCGG GGAGGGCTCG GGGGAGGGGC GCGGCGGCCC CCGGAGCGCC GGCGGCTGTC GAGGCGCGGC GAGCCGCAGC CATTGCCTTT TATGGTAATC GTGCGAGAGG GCGCAGGGAC TTCCTTTGTC CCAAATCTGT GCGGAGCCGA AATCTGGGAG GCGCCGCCGC ACCCCCTCTA GCGGGCGCGG GGCGAAGCGG TGCGGCGCCG GCAGGAAGGA AATGGGCGGG GAGGGCCTTC GTGCGTCGCC GCGCCGCCGT CCCCTTCTCC CTCTCCAGCC TCGGGGCTGT CCGCGGGGGG ACGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT GTGACCGGCG GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG ATCCTTAATT AAGTCTCATC ATTTTGGCAA AGAATTGATT TGATACCGCG GGCCCTAAGC CACCATGAAC TTGGAGCGGG TATCCAATGA GGAGAAGTTG AACCTGTGCC GGAAGTACTA TCTTGGTGGA TTTGCGTTCC TGCCTTTTCT TTGGTTAGTC AACATTTTCT GGTTCTTCAG AGAGGCGTTC CTCGCCCCAG CCTACACAGA GCAGAGCCAA ATCAAAGGCT ATGTTTGGCG CTCAGCTGTG GGCTTCCTCT TCTGGGTGAT CATTCTCGCC ACCTGGATCA CCATCTTCCA GATCTACCGG CCCCGCTGGG GCGCTCTTGG GGACTACCTC TCCTTCACCA TTCCCCTGGG CACTCCCTAA AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAATCAT CGTCCTTTCC TTGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTC CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGCCGC CTCCCCGCCA CCGGTTAGGG GCCCGTTTAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCGGCGG CCGCAGATGG GCGGGAGTCT TCTGGGCAGG CTTAAAGGCT AACCTGGTGT GTGGGCGTTG TCCTGCAGGG GAATTGAACA GGTGTAAAAT TGGAGGGACA AGACTTCCCA CAGATTTTCG GTTTTGTCGG GAAGTTTTTT AATAGGGGCA AATAAGGAAA ATGGGAGGAT AGGTAGTCAT CTGGGGTTTT ATGCAGCAAA ACTACAGGTT ATTATTGCTT GTGATCCGCC TCGGAGTATT TTCCATCGAG GTAGATTAAA GACATGCTCA CCCGAGTTTT ATACTCTCCT GCTTGAGATC CTTACTACAG TATGAAATTA CAGTGTCGCG AGTTAGACTA TGTAAGCAGA ATTTTAATCA TTTTTAAAGA GCCCAGTACT TCATATCCAT TTCTCCCGCT CCTTCTGCAG CCTTATCAAA AGGTATTTTA GAACACTCAT TTTAGCCCCA TTTTCATTTA TTATACTGGC TTATCCAACC CCTAGACAGA GCATTGGCAT TTTCCCTTTC CTGATCTTAG AAGTCTGATG ACTCATGAAA CCAGACAGAT TAGTTACATA CACCACAAAT CGAGGCTGTA GCTGGGGCCT CAACACTGCA GTTCTTTTAT AACTCCTTAG TACACTTTTT GTTGATCCTT TGCCTTGATC CTTAATTTTC AGTGTCTATC ACCTCTCCCG TCAGGTGGTG TTCCACATTT GGGCCTATTC TCAGTCCAGG GAGTTTTACA ACAATAGATG TATTGAGAAT CCAACCTAAA GCTTAACTTT CCACTCCCAT GAATGCCTCT CTCCTTTTTC TCCAGCTAGC CTCGAGCATG CATCTAGAGG GCCCAATTCG CCCTATAGTG AGTCGTATTA CAATTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA ATTCAGGGCG CAAGGGCTGC TAAAGGAAGC GGAACACGTA GAAAGCCAGT CCGCAGAAAC GGTGCTGACC CCGGATGAAT GTCAGCTACT GGGCTATCTG GACAAGGGAA AACGCAAGCG CAAAGAGAAA GCAGGTAGCT TGCAGTGGGC TTACATGGCG ATAGCTAGAC TGGGCGGTTT TATGGACAGC AAGCGAACCG GAATTGCCAG CTGGGGCGCC CTCTGGTAAG GTTGGGAAGC CCTGCAAAGT AAACTGGATG GCTTTCTTGC CGCCAAGGAT CTGATGGCGC AGGGGATCAA GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCC CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAG
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