pCMV-mCherry-PARP1(human)-Neo
$99.00
In stock
SKU
PL36449
NM_001618.4
100μg liquid plasmid(Instantly):$120
| name | pCMV-mCherry-PARP1(human)-Neo | another name | apCMV-mCherry-PARP1(human)-Neo |
| Insert Size (bp) | 3045bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | Red |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGGATAACA TGGCCATCAT CAAGGAGTTC ATGCGCTTCA AGGTGCACAT GGAGGGCTCC GTGAACGGCC ACGAGTTCGA GATCGAGGGC GAGGGCGAGG GCCGCCCCTA CGAGGGCACC CAGACCGCCA AGCTGAAGGT GACCAAGGGT GGCCCCCTGC CCTTCGCCTG GGACATCCTG TCCCCTCAGT TCATGTACGG CTCCAAGGCC TACGTGAAGC ACCCCGCCGA CATCCCCGAC TACTTGAAGC TGTCCTTCCC CGAGGGCTTC AAGTGGGAGC GCGTGATGAA CTTCGAGGAC GGCGGCGTGG TGACCGTGAC CCAGGACTCC TCCCTGCAGG ACGGCGAGTT CATCTACAAG GTGAAGCTGC GCGGCACCAA CTTCCCCTCC GACGGCCCCG TAATGCAGAA GAAGACCATG GGCTGGGAGG CCTCCTCCGA GCGGATGTAC CCCGAGGACG GCGCCCTGAA GGGCGAGATC AAGCAGAGGC TGAAGCTGAA GGACGGCGGC CACTACGACG CTGAGGTCAA GACCACCTAC AAGGCCAAGA AGCCCGTGCA GCTGCCCGGC GCCTACAACG TCAACATCAA GTTGGACATC ACCTCCCACA ACGAGGACTA CACCATCGTG GAACAGTACG AACGCGCCGA GGGCCGCCAC TCCACCGGCG GCATGGACGA GCTGTACAAG TCCGGACTCA GATCTCGAGC TGGAGGTGGA GGTAGTGGAG GAGGCGGAAG CAAGCTTATG GCGGAGTCTT CGGATAAGCT CTATCGAGTC GAGTACGCCA AGAGCGGGCG CGCCTCTTGC AAGAAATGCA GCGAGAGCAT CCCCAAGGAC TCGCTCCGGA TGGCCATCAT GGTGCAGTCG CCCATGTTTG ATGGAAAAGT CCCACACTGG TACCACTTCT CCTGCTTCTG GAAGGTGGGC CACTCCATCC GGCACCCTGA CGTTGAGGTG GATGGGTTCT CTGAGCTTCG GTGGGATGAC CAGCAGAAAG TCAAGAAGAC AGCGGAAGCT GGAGGAGTGA CAGGCAAAGG CCAGGATGGA ATTGGTAGCA AGGCAGAGAA GACTCTGGGT GACTTTGCAG CAGAGTATGC CAAGTCCAAC AGAAGTACGT GCAAGGGGTG TATGGAGAAG ATAGAAAAGG GCCAGGTGCG CCTGTCCAAG AAGATGGTGG ACCCGGAGAA GCCACAGCTA GGCATGATTG ACCGCTGGTA CCATCCAGGC TGCTTTGTCA AGAACAGGGA GGAGCTGGGT TTCCGGCCCG AGTACAGTGC GAGTCAGCTC AAGGGCTTCA GCCTCCTTGC TACAGAGGAT AAAGAAGCCC TGAAGAAGCA GCTCCCAGGA GTCAAGAGTG AAGGAAAGAG AAAAGGCGAT GAGGTGGATG GAGTGGATGA AGTGGCGAAG AAGAAATCTA AAAAAGAAAA AGACAAGGAT AGTAAGCTTG AAAAAGCCCT AAAGGCTCAG AACGACCTGA TCTGGAACAT CAAGGACGAG CTAAAGAAAG TGTGTTCAAC TAATGACCTG AAGGAGCTAC TCATCTTCAA CAAGCAGCAA GTGCCTTCTG GGGAGTCGGC GATCTTGGAC CGAGTAGCTG ATGGCATGGT GTTCGGTGCC CTCCTTCCCT GCGAGGAATG CTCGGGTCAG CTGGTCTTCA AGAGCGATGC CTATTACTGC ACTGGGGACG TCACTGCCTG GACCAAGTGT ATGGTCAAGA CACAGACACC CAACCGGAAG GAGTGGGTAA CCCCAAAGGA ATTCCGAGAA ATCTCTTACC TCAAGAAATT GAAGGTTAAA AAACAGGACC GTATATTCCC CCCAGAAACC AGCGCCTCCG TGGCGGCCAC GCCTCCGCCC TCCACAGCCT CGGCTCCTGC TGCTGTGAAC TCCTCTGCTT CAGCAGATAA GCCATTATCC AACATGAAGA TCCTGACTCT CGGGAAGCTG TCCCGGAACA AGGATGAAGT GAAGGCCATG ATTGAGAAAC TCGGGGGGAA GTTGACGGGG ACGGCCAACA AGGCTTCCCT GTGCATCAGC ACCAAAAAGG AGGTGGAAAA GATGAATAAG AAGATGGAGG AAGTAAAGGA AGCCAACATC CGAGTTGTGT CTGAGGACTT CCTCCAGGAC GTCTCCGCCT CCACCAAGAG CCTTCAGGAG TTGTTCTTAG CGCACATCTT GTCCCCTTGG GGGGCAGAGG TGAAGGCAGA GCCTGTTGAA GTTGTGGCCC CAAGAGGGAA GTCAGGGGCT GCGCTCTCCA AAAAAAGCAA GGGCCAGGTC AAGGAGGAAG GTATCAACAA ATCTGAAAAG AGAATGAAAT TAACTCTTAA AGGAGGAGCA GCTGTGGATC CTGATTCTGG ACTGGAACAC TCTGCGCATG TCCTGGAGAA AGGTGGGAAG GTCTTCAGTG CCACCCTTGG CCTGGTGGAC ATCGTTAAAG GAACCAACTC CTACTACAAG CTGCAGCTTC TGGAGGACGA CAAGGAAAAC AGGTATTGGA TATTCAGGTC CTGGGGCCGT GTGGGTACGG TGATCGGTAG CAACAAACTG GAACAGATGC CGTCCAAGGA GGATGCCATT GAGCACTTCA TGAAATTATA TGAAGAAAAA ACCGGGAACG CTTGGCACTC CAAAAATTTC ACGAAGTATC CCAAAAAGTT CTACCCCCTG GAGATTGACT ATGGCCAGGA TGAAGAGGCA GTGAAGAAGC TGACAGTAAA TCCTGGCACC AAGTCCAAGC TCCCCAAGCC AGTTCAGGAC CTCATCAAGA TGATCTTTGA TGTGGAAAGT ATGAAGAAAG CCATGGTGGA GTATGAGATC GACCTTCAGA AGATGCCCTT GGGGAAGCTG AGCAAAAGGC AGATCCAGGC CGCATACTCC ATCCTCAGTG AGGTCCAGCA GGCGGTGTCT CAGGGCAGCA GCGACTCTCA GATCCTGGAT CTCTCAAATC GCTTTTACAC CCTGATCCCC CACGACTTTG GGATGAAGAA GCCTCCGCTC CTGAACAATG CAGACAGTGT GCAGGCCAAG GTGGAAATGC TTGACAACCT GCTGGACATC GAGGTGGCCT ACAGTCTGCT CAGGGGAGGG TCTGATGATA GCAGCAAGGA TCCCATCGAT GTCAACTATG AGAAGCTCAA AACTGACATT AAGGTGGTTG ACAGAGATTC TGAAGAAGCC GAGATCATCA GGAAGTATGT TAAGAACACT CATGCAACCA CACACAATGC GTATGACTTG GAAGTCATCG ATATCTTTAA GATAGAGCGT GAAGGCGAAT GCCAGCGTTA CAAGCCCTTT AAGCAGCTTC ATAACCGAAG ATTGCTGTGG CACGGGTCCA GGACCACCAA CTTTGCTGGG ATCCTGTCCC AGGGTCTTCG GATAGCCCCG CCTGAAGCGC CCGTGACAGG CTACATGTTT GGTAAAGGGA TCTATTTCGC TGACATGGTC TCCAAGAGTG CCAACTACTG CCATACGTCT CAGGGAGACC CAATAGGCTT AATCCTGTTG GGAGAAGTTG CCCTTGGAAA CATGTATGAA CTGAAGCACG CTTCACATAT CAGCAAGTTA CCCAAGGGCA AGCACAGTGT CAAAGGTTTG GGCAAAACTA CCCCTGATCC TTCAGCTAAC ATTAGTCTGG ATGGTGTAGA CGTTCCTCTT GGGACCGGGA TTTCATCTGG TGTGAATGAC ACCTCTCTAC TATATAACGA GTACATTGTC TATGATATTG CTCAGGTAAA TCTGAAGTAT CTGCTGAAAC TGAAATTCAA TTTTAAGACC TCCCTGTGGT AAGGATCCAC CGGATCTAGA TAACTGATCA TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTAACGCGTA AATTGTAAGC GTTAATATTT TGTTAAAATT CGCGTTAAAT TTTTGTTAAA TCAGCTCATT TTTTAACCAA TAGGCCGAAA TCGGCAAAAT CCCTTATAAA TCAAAAGAAT AGACCGAGAT AGGGTTGAGT GTTGTTCCAG TTTGGAACAA GAGTCCACTA TTAAAGAACG TGGACTCCAA CGTCAAAGGG CGAAAAACCG TCTATCAGGG CGATGGCCCA CTACGTGAAC CATCACCCTA ATCAAGTTTT TTGGGGTCGA GGTGCCGTAA AGCACTAAAT CGGAACCCTA AAGGGAGCCC CCGATTTAGA GCTTGACGGG GAAAGCCGGC GAACGTGGCG AGAAAGGAAG GGAAGAAAGC GAAAGGAGCG GGCGCTAGGG CGCTGGCAAG TGTAGCGGTC ACGCTGCGCG TAACCACCAC ACCCGCCGCG CTTAATGCGC CGCTACAGGG CGCGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTCCTG AGGCGGAAAG AACCAGCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAGATCG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCTAGG GGGAGGCTAA CTGAAACACG GAAGGAGACA ATACCGGAAG GAACCCGCGC TATGACGGCA ATAAAAAGAC AGAATAAAAC GCACGGTGTT GGGTCGTTTG TTCATAAACG CGGGGTTCGG TCCCAGGGCT GGCACTCTGT CGATACCCCA CCGAGACCCC ATTGGGGCCA ATACGCCCGC GTTTCTTCCT TTTCCCCACC CCACCCCCCA AGTTCGGGTG AAGGCCCAGG GCTCGCAGCC AACGTCGGGG CGGCAGGCCC TGCCATAGCC TCAGGTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCAT GCAT
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