NM_002473.6;BDPLT6; DFNA17; EPSTS; FTNS; MATINS; MHA; NMHC-II-A; NMMHC-IIA; NMMHCA
100μg liquid plasmid(Instantly):$149
| name | pCMV-EGFP-MYH9(human)-Puro | another name | apCMV-EGFP-MYH9(human)-Puro |
| Insert Size (bp) | 5883bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGCC ACCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG AAGCTTGGTG GAGGCGGATC AGGAGGCGGT GGATCTATGG CACAGCAAGC TGCCGATAAG TATCTCTATG TGGATAAAAA CTTCATCAAC AATCCGCTGG CCCAGGCCGA CTGGGCTGCC AAGAAGCTGG TATGGGTGCC TTCCGACAAG AGTGGCTTTG AGCCAGCCAG CCTCAAGGAG GAGGTGGGCG AAGAGGCCAT CGTGGAGCTG GTGGAGAATG GGAAGAAGGT GAAGGTGAAC AAGGATGACA TCCAGAAGAT GAACCCGCCC AAGTTCTCCA AGGTGGAGGA CATGGCAGAG CTCACGTGCC TCAACGAAGC CTCGGTGCTG CACAACCTCA AGGAGCGTTA CTACTCAGGG CTCATCTACA CCTATTCAGG CCTGTTCTGT GTGGTCATCA ATCCTTACAA GAACCTGCCC ATCTACTCTG AAGAGATTGT GGAAATGTAC AAGGGCAAGA AGAGGCACGA GATGCCCCCT CACATCTATG CCATCACAGA CACCGCCTAC AGGAGTATGA TGCAAGACCG AGAAGATCAA TCCATCTTGT GCACTGGTGA ATCTGGAGCT GGCAAGACGG AGAACACCAA GAAGGTCATC CAGTATCTGG CGTACGTGGC GTCCTCGCAC AAGAGCAAGA AGGACCAGGG CGAGCTGGAG CGGCAGCTGC TGCAGGCCAA CCCCATCCTG GAGGCCTTCG GGAACGCCAA GACCGTGAAG AATGACAACT CCTCCCGCTT CGGCAAATTC ATTCGCATCA ACTTTGATGT CAATGGCTAC ATTGTTGGAG CCAACATTGA GACTTATCTT TTGGAGAAAT CTCGTGCTAT CCGCCAAGCC AAGGAAGAAC GGACCTTCCA CATCTTCTAT TATCTCCTGT CTGGGGCTGG AGAGCACCTG AAGACCGATC TCCTGTTGGA GCCGTACAAC AAATACCGCT TCCTGTCCAA TGGACACGTC ACCATCCCCG GGCAGCAGGA CAAGGACATG TTCCAGGAGA CCATGGAGGC CATGAGGATT ATGGGCATCC CAGAAGAGGA GCAAATGGGC CTGCTGCGGG TCATCTCAGG GGTTCTTCAG CTCGGCAACA TCGTCTTCAA GAAGGAGCGG AACACTGACC AGGCGTCCAT GCCCGACAAC ACAGCTGCCC AAAAGGTGTC CCATCTCTTG GGTATCAATG TGACCGATTT CACCAGAGGA ATCCTCACCC CGCGCATCAA GGTGGGACGG GATTACGTCC AGAAGGCGCA GACTAAAGAG CAGGCTGACT TTGCCATCGA GGCCTTGGCC AAGGCGACCT ATGAGCGGAT GTTCCGCTGG CTGGTGCTGC GCATCAACAA GGCTCTGGAC AAGACCAAGA GGCAGGGCGC CTCCTTCATC GGGATCCTGG ACATTGCCGG CTTCGAGATC TTTGATCTGA ACTCGTTTGA GCAGCTGTGC ATCAATTACA CCAATGAGAA GCTGCAGCAG CTCTTCAACC ACACCATGTT CATCCTGGAG CAGGAGGAGT ACCAGCGCGA GGGCATCGAG TGGAACTTCA TCGACTTTGG CCTCGACCTG CAGCCCTGCA TCGACCTCAT TGAGAAGCCA GCAGGCCCCC CGGGCATTCT GGCCCTGCTG GACGAGGAGT GCTGGTTCCC CAAAGCCACC GACAAGAGCT TCGTGGAGAA GGTGATGCAG GAGCAGGGCA CCCACCCCAA GTTCCAGAAG CCCAAGCAGC TGAAGGACAA AGCTGATTTC TGCATTATCC ACTATGCCGG CAAGGTGGAT TACAAAGCTG ACGAGTGGCT GATGAAGAAC ATGGATCCCC TGAATGACAA CATCGCCACA CTGCTCCACC AGTCCTCTGA CAAGTTTGTC TCGGAGCTGT GGAAGGATGT GGACCGCATC ATCGGCCTGG ACCAGGTGGC CGGCATGTCG GAGACCGCAC TGCCCGGGGC CTTCAAGACG CGGAAGGGCA TGTTCCGCAC TGTGGGGCAG CTTTACAAGG AGCAGCTGGC CAAGCTGATG GCTACGCTGA GGAACACGAA CCCCAACTTT GTCCGCTGCA TCATCCCCAA CCACGAGAAG AAGGCCGGCA AGCTGGACCC GCATCTCGTG CTGGACCAGC TGCGCTGCAA CGGTGTTCTC GAGGGCATCC GTATCTGCCG CCAGGGCTTC CCCAACAGGG TGGTCTTCCA GGAGTTTCGG CAGAGATATG AGATCCTGAC TCCAAACTCC ATTCCCAAGG GTTTCATGGA CGGGAAGCAG GCGTGCGTGC TCATGATAAA AGCCCTGGAG CTCGACAGCA ATCTGTACCG CATTGGCCAG AGCAAAGTCT TCTTCCGTGC CGGTGTGCTG GCCCACCTGG AGGAGGAGCG AGACCTGAAG ATCACCGACG TCATCATAGG GTTCCAGGCC TGCTGCAGGG GCTACCTGGC CAGGAAAGCA TTTGCCAAGC GGCAGCAGCA GCTTACCGCC ATGAAGGTCC TCCAGCGGAA CTGCGCTGCC TACCTGAAGC TGCGGAACTG GCAGTGGTGG CGGCTCTTCA CCAAGGTCAA GCCGCTGCTG CAGGTGAGCC GGCAGGAGGA GGAGATGATG GCCAAGGAGG AGGAGCTGGT GAAGGTCAGA GAGAAGCAGC TGGCTGCGGA GAACAGGCTC ACGGAGATGG AGACGCTGCA GTCTCAGCTC ATGGCAGAGA AATTGCAGCT GCAGGAGCAG CTCCAGGCAG AAACCGAGCT GTGTGCCGAG GCTGAGGAGC TCCGGGCCCG CCTGACCGCC AAGAAGCAGG AATTAGAAGA GATCTGCCAT GACCTAGAGG CCAGGGTGGA GGAGGAGGAG GAGCGCTGCC AGCACCTGCA GGCGGAGAAG AAGAAGATGC AGCAGAACAT CCAGGAGCTT GAGGAGCAGC TGGAGGAGGA GGAGAGCGCC CGGCAGAAGC TGCAGCTGGA GAAGGTGACC ACCGAGGCGA AGCTGAAAAA GCTGGAGGAG GAGCAGATCA TCCTGGAGGA CCAGAACTGC AAGCTGGCCA AGGAAAAGAA ACTGCTGGAA GACAGAATAG CTGAGTTCAC CACCAACCTC ACAGAAGAGG AGGAGAAATC TAAGAGCCTC GCCAAGCTCA AGAACAAGCA TGAGGCAATG ATCACTGACT TGGAAGAGCG CCTCCGCAGG GAGGAGAAGC AGCGACAGGA GCTGGAGAAG ACCCGCCGGA AGCTGGAGGG AGACTCCACA GACCTCAGCG ACCAGATCGC CGAGCTCCAG GCCCAGATCG CGGAGCTCAA GATGCAGCTG GCCAAGAAAG AGGAGGAGCT CCAGGCCGCC CTGGCCAGAG TGGAAGAGGA AGCTGCCCAG AAGAACATGG CCCTCAAGAA GATCCGGGAG CTGGAATCTC AGATCTCTGA ACTCCAGGAA GACCTGGAGT CTGAGCGTGC TTCCAGGAAT AAAGCTGAGA AGCAGAAACG GGACCTTGGG GAAGAGCTAG AGGCTCTGAA AACAGAGTTG GAGGACACGC TGGATTCCAC AGCTGCCCAG CAGGAGCTCA GGTCAAAACG TGAGCAGGAG GTGAACATCC TGAAGAAGAC CCTGGAGGAG GAGGCCAAGA CCCACGAGGC CCAGATCCAG GAGATGAGGC AGAAGCACTC ACAGGCCGTG GAGGAGCTGG CGGAGCAGCT GGAGCAGACG AAGCGGGTGA AAGCAAACCT CGAGAAGGCA AAGCAGACTC TGGAGAACGA GCGGGGGGAG CTGGCCAACG AGGTGAAGGT GCTGCTGCAG GGCAAAGGGG ACTCGGAGCA CAAGCGCAAG AAAGTGGAGG CGCAGCTGCA GGAGCTGCAG GTCAAGTTCA ACGAGGGAGA GCGCGTGCGC ACAGAGCTGG CCGACAAGGT CACCAAGCTG CAGGTGGAGC TGGACAACGT GACCGGGCTT CTCAGCCAGT CCGACAGCAA GTCCAGCAAG CTCACCAAGG ACTTCTCCGC GCTGGAGTCC CAGCTGCAGG ACACTCAGGA GCTGCTGCAG GAGGAGAACC GGCAGAAGCT GAGCCTGAGC ACCAAGCTCA AGCAGGTGGA GGACGAGAAG AATTCCTTCC GGGAGCAGCT GGAGGAGGAG GAGGAGGCCA AGCACAACCT GGAGAAGCAG ATCGCCACCC TCCATGCCCA GGTGGCCGAC ATGAAAAAGA AGATGGAGGA CAGTGTGGGG TGCCTGGAAA CTGCTGAGGA GGTGAAGAGG AAGCTCCAGA AGGACCTGGA GGGCCTGAGC CAGCGGCACG AGGAGAAGGT GGCCGCCTAC GACAAGCTGG AGAAGACCAA GACGCGGCTG CAGCAGGAGC TGGACGACCT GCTGGTGGAC CTGGACCACC AGCGCCAGAG CGCGTGCAAC CTGGAGAAGA AGCAGAAGAA GTTTGACCAG CTCCTGGCGG AGGAGAAGAC CATCTCTGCC AAGTATGCAG AGGAGCGCGA CCGGGCTGAG GCGGAGGCCC GAGAGAAGGA GACCAAGGCT CTGTCGCTGG CCCGGGCCCT GGAGGAAGCC ATGGAGCAGA AGGCGGAGCT GGAGCGGCTC AACAAGCAGT TCCGCACGGA GATGGAGGAC CTTATGAGCT CCAAGGATGA TGTGGGCAAG AGTGTCCACG AGCTGGAGAA GTCCAAGCGG GCCCTAGAGC AGCAGGTGGA GGAGATGAAG ACGCAGCTGG AAGAGCTGGA GGACGAGCTG CAGGCCACCG AAGATGCCAA GCTGCGGTTG GAGGTCAACC TGCAGGCCAT GAAGGCCCAG TTCGAGCGGG ACCTGCAGGG CCGGGACGAG CAGAGCGAGG AGAAGAAGAA GCAGCTGGTC AGACAGGTGC GGGAGATGGA GGCAGAGCTG GAGGACGAGA GGAAGCAGCG CTCGATGGCA GTGGCCGCCC GGAAGAAGCT GGAGATGGAC CTGAAGGACC TGGAGGCGCA CATCGACTCG GCCAACAAGA ACCGGGACGA AGCCATCAAA CAGCTGCGGA AGCTGCAGGC CCAGATGAAG GACTGCATGC GCGAGCTGGA TGACACCCGC GCCTCTCGTG AGGAGATCCT GGCCCAGGCC AAAGAGAACG AGAAGAAGCT GAAGAGCATG GAGGCCGAGA TGATCCAGTT GCAGGAGGAA CTGGCAGCCG CGGAGCGTGC CAAGCGCCAG GCCCAGCAGG AGCGGGATGA GCTGGCTGAC GAGATCGCCA ACAGCAGCGG CAAAGGAGCC CTGGCGTTAG AGGAGAAGCG GCGTCTGGAG GCCCGCATCG CCCAGCTGGA GGAGGAGCTG GAGGAGGAGC AGGGCAACAC GGAGCTGATC AACGACCGGC TGAAGAAGGC CAACCTGCAG ATCGACCAGA TCAACACCGA CCTGAACCTG GAGCGCAGCC ACGCCCAGAA GAACGAGAAT GCTCGGCAGC AGCTGGAACG CCAGAACAAG GAGCTTAAGG TCAAGCTGCA GGAGATGGAG GGCACTGTCA AGTCCAAGTA CAAGGCCTCC ATCACCGCCC TCGAGGCCAA GATTGCACAG CTGGAGGAGC AGCTGGACAA CGAGACCAAG GAGCGCCAGG CAGCCTGCAA ACAGGTGCGT CGGACCGAGA AGAAGCTGAA GGATGTGCTG CTGCAGGTGG ATGACGAGCG GAGGAACGCC GAGCAGTACA AGGACCAGGC CGACAAGGCA TCTACCCGCC TGAAGCAGCT CAAGCGGCAG CTGGAGGAGG CCGAAGAGGA GGCCCAGCGG GCCAACGCCT CCCGCCGGAA ACTGCAGCGC GAGCTGGAGG ACGCCACTGA GACGGCCGAT GCCATGAACC GCGAAGTCAG CTCCCTAAAG AACAAGCTCA GGCGCGGGGA CCTGCCGTTT GTCGTGCCCC GCCGAATGGC CCGGAAAGGC GCCGGGGATG GCTCCGACGA AGAGGTAGAT GGCAAAGCGG ATGGGGCTGA GGCCAAACCT GCCGAATAAG AATTCTGCAG ATATCCAGCA CAGTGGCGGC CGCTCGAGTC TAGAGGGCCC GTTTAAACCC GCTGATCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG GGCTCTATGG CTTCTGAGGC GGAAAGAACC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTAATTCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGACCG AGTACAAGCC CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCCGGGCC GTACGCACCC TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGACCCG GACCGCCACA TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CGACGGATCG GGAGATCTCC CGATCCCCTA TGGT
