pCMV-LRP6(human)-3×FLAG-Neo
$113.00
In stock
SKU
PL36248
NM_002336.3;ADCAD2; STHAG7
100μg liquid plasmid(Instantly):$134
| name | pCMV-LRP6(human)-3×FLAG-Neo | another name | apCMV-LRP6(human)-3×FLAG-Neo |
| Insert Size (bp) | 4839bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCATGGG GGCCGTCCTG AGGAGCCTCC TGGCCTGCAG CTTCTGTGTG CTCCTGAGAG CGGCCCCTTT GTTGCTTTAT GCAAACAGAC GGGACTTGCG ATTGGTTGAT GCTACAAATG GCAAAGAGAA TGCTACGATT GTAGTTGGAG GCTTGGAGGA TGCAGCTGCG GTGGACTTTG TGTTTAGTCA TGGCTTGATA TACTGGAGTG ATGTCAGCGA AGAAGCCATT AAACGAACAG AATTTAACAA AACTGAGAGT GTGCAGAATG TTGTTGTTTC TGGATTATTG TCCCCCGATG GGCTGGCATG TGATTGGCTT GGAGAAAAAT TGTACTGGAC AGATTCTGAA ACTAATCGGA TTGAAGTTTC TAATTTAGAT GGATCTTTAC GAAAAGTTTT ATTTTGGCAA GAGTTGGATC AACCCAGAGC TATTGCCTTA GATCCTTCAA GTGGGTTCAT GTACTGGACA GACTGGGGAG AAGTGCCAAA GATAGAACGT GCTGGAATGG ATGGTTCAAG TCGCTTCATT ATAATAAACA GTGAAATTTA CTGGCCAAAT GGACTGACTT TGGATTATGA AGAACAAAAG CTTTATTGGG CAGATGCAAA ACTTAATTTC ATCCACAAAT CAAATCTGGA TGGAACAAAT CGGCAGGCAG TGGTTAAAGG TTCCCTTCCA CATCCTTTTG CCTTGACGTT ATTTGAGGAC ATATTGTACT GGACTGACTG GAGCACACAC TCCATTTTGG CTTGCAACAA GTATACTGGT GAGGGTCTGC GTGAAATCCA TTCTGACATC TTCTCTCCCA TGGATATACA TGCCTTCAGC CAACAGAGGC AGCCAAATGC CACAAATCCA TGTGGAATTG ACAATGGGGG TTGTTCCCAT TTGTGTTTGA TGTCTCCAGT CAAGCCTTTT TATCAGTGTG CTTGCCCCAC TGGGGTCAAA CTCCTGGAGA ATGGAAAAAC CTGCAAAGAT GGTGCCACAG AATTATTGCT TTTAGCTCGA AGGACAGACT TGAGACGCAT TTCTTTGGAT ACACCAGATT TTACAGACAT TGTTCTGCAG TTAGAAGACA TCCGTCATGC CATTGCCATA GATTACGATC CTGTGGAAGG CTACATCTAC TGGACTGATG ATGAAGTGAG GGCCATACGC CGTTCATTTA TAGATGGATC TGGCAGTCAG TTTGTGGTCA CTGCTCAAAT TGCCCATCCT GATGGTATTG CTGTGGACTG GGTTGCACGA AATCTTTATT GGACAGACAC TGGCACTGAT CGAATAGAAG TGACAAGGCT CAATGGGACC ATGAGGAAGA TCTTGATTTC AGAGGACTTA GAGGAACCCC GGGCTATTGT GTTAGATCCC ATGGTTGGGT ACATGTATTG GACTGACTGG GGAGAAATTC CGAAAATTGA GCGAGCAGCT CTGGATGGTT CTGACCGTGT AGTATTGGTT AACACTTCTC TTGGTTGGCC AAATGGTTTA GCCTTGGATT ATGATGAAGG CAAAATATAC TGGGGAGATG CCAAAACAGA CAAGATTGAG GTTATGAATA CTGATGGCAC TGGGAGACGA GTACTAGTGG AAGACAAAAT TCCTCACATA TTTGGATTTA CTTTGTTGGG TGACTATGTT TACTGGACTG ACTGGCAGAG GCGTAGCATT GAAAGAGTTC ATAAACGAAG TGCAGAGAGG GAAGTGATCA TAGATCAGCT GCCTGACCTC ATGGGCCTAA AGGCTACAAA TGTTCATCGA GTGATTGGTT CCAACCCCTG TGCTGAGGAA AACGGGGGAT GTAGCCATCT CTGCCTCTAT AGACCTCAGG GCCTTCGCTG TGCTTGCCCT ATTGGCTTTG AACTCATCAG TGACATGAAG ACCTGCATTG TCCCAGAGGC TTTCCTTTTG TTTTCACGGA GAGCAGATAT CAGACGAATT TCTCTGGAAA CAAACAATAA TAATGTGGCT ATTCCACTCA CTGGTGTCAA AGAAGCTTCT GCTTTGGATT TTGATGTGAC AGACAACCGA ATTTATTGGA CTGATATATC ACTCAAGACC ATCAGCAGAG CCTTTATGAA TGGCAGTGCA CTGGAACATG TGGTAGAATT CGGCTTAGAT TATCCAGAAG GCATGGCAGT AGACTGGCTT GGGAAGAACT TGTACTGGGC AGACACAGGA ACGAATCGAA TTGAGGTGTC AAAGTTGGAT GGGCAGCACC GACAAGTTTT GGTGTGGAAA GACCTAGATA GTCCCAGAGC TCTCGCGTTG GACCCTGCCG AAGGATTTAT GTATTGGACT GAATGGGGTG GAAAACCTAA GATAGACAGA GCTGCAATGG ATGGAAGTGA ACGTACTACC TTAGTTCCAA ATGTGGGGCG GGCAAACGGC CTAACTATTG ATTATGCTAA AAGGAGGCTT TATTGGACAG ACCTGGACAC CAACTTAATA GAATCTTCAA ATATGCTTGG GCTCAACCGT GAAGTTATAG CAGATGACTT GCCTCATCCT TTTGGCTTAA CTCAGTACCA AGATTATATC TACTGGACGG ACTGGAGCCG ACGCAGCATT GAGCGTGCCA ACAAAACCAG TGGCCAAAAC CGCACCATCA TTCAGGGCCA TTTGGATTAT GTGATGGACA TCCTCGTCTT TCACTCATCT CGACAGTCAG GGTGGAATGA ATGTGCTTCC AGCAATGGGC ACTGCTCCCA CCTCTGCTTG GCTGTGCCAG TTGGGGGTTT TGTTTGTGGA TGCCCTGCCC ACTACTCTCT TAATGCTGAC AACAGGACTT GTAGTGCTCC TACGACTTTC CTGCTCTTCA GTCAAAAGAG TGCCATCAAC CGCATGGTGA TTGATGAACA ACAGAGCCCC GACATCATCC TTCCCATCCA CAGCCTTCGG AATGTCCGGG CCATTGACTA TGACCCACTG GACAAGCAAC TCTATTGGAT TGACTCACGA CAAAACATGA TCCGAAAGGC ACAAGAAGAT GGCAGCCAGG GCTTTACTGT GGTTGTGAGC TCAGTTCCGA GTCAGAACCT GGAAATACAA CCCTATGACC TCAGCATTGA TATTTACAGC CGCTACATCT ACTGGACTTG TGAGGCTACC AATGTCATTA ATGTGACAAG ATTAGATGGG AGATCAGTTG GAGTGGTGCT GAAAGGCGAG CAGGACAGAC CTCGAGCCGT TGTGGTAAAC CCAGAGAAAG GGTATATGTA TTTTACCAAT CTTCAGGAAA GGTCTCCTAA AATTGAACGG GCTGCTTTGG ATGGGACAGA ACGGGAGGTC CTCTTTTTCA GTGGCTTAAG TAAACCAATT GCTTTAGCCC TTGATAGCAG GCTGGGCAAG CTCTTTTGGG CTGATTCAGA TCTCCGGCGA ATTGAAAGCA GTGATCTCTC AGGTGCTAAC CGGATAGTAT TAGAAGACTC CAATATCTTG CAGCCTGTGG GACTTACTGT GTTTGAAAAC TGGCTCTATT GGATTGATAA ACAGCAGCAA ATGATTGAAA AAATTGACAT GACAGGTCGA GAGGGTAGAA CCAAAGTCCA AGCTCGAATT GCCCAGCTTA GTGACATTCA TGCAGTAAAG GAGCTGAACC TTCAAGAATA CAGACAGCAC CCTTGTGCTC AGGATAATGG TGGCTGTTCA CATATTTGTC TTGTAAAGGG GGATGGTACT ACAAGGTGTT CTTGCCCCAT GCACCTGGTT CTACTTCAAG ATGAGCTATC ATGTGGAGAA CCTCCAACAT GTTCTCCTCA GCAGTTTACT TGTTTCACGG GGGAAATTGA CTGTATCCCT GTGGCTTGGC GGTGCGATGG GTTTACTGAA TGTGAAGACC ACAGTGATGA ACTCAATTGT CCTGTATGCT CAGAGTCCCA GTTCCAGTGT GCCAGTGGGC AGTGTATTGA TGGTGCCCTC CGATGCAATG GAGATGCAAA CTGCCAGGAC AAATCAGATG AGAAGAACTG TGAAGTGCTT TGTTTAATTG ATCAGTTCCG CTGTGCCAAT GGTCAGTGCA TTGGAAAGCA CAAGAAGTGT GATCATAATG TGGATTGCAG TGACAAGTCA GATGAACTGG ATTGTTATCC GACTGAAGAA CCAGCACCAC AGGCCACCAA TACAGTTGGT TCTGTTATTG GCGTAATTGT CACCATTTTT GTGTCTGGAA CTGTATACTT TATCTGCCAG AGGATGTTGT GTCCACGTAT GAAGGGAGAT GGGGAAACTA TGACTAATGA CTATGTAGTT CATGGACCAG CTTCTGTGCC TCTTGGTTAT GTGCCACACC CAAGTTCTTT GTCAGGATCT CTTCCAGGAA TGTCTCGAGG TAAATCAATG ATCAGCTCCC TCAGTATCAT GGGGGGAAGC AGTGGACCCC CCTATGACCG AGCCCATGTT ACAGGAGCAT CATCAAGTAG TTCTTCAAGC ACCAAAGGCA CTTACTTCCC TGCAATTTTG AACCCTCCAC CATCCCCAGC CACAGAGCGA TCACATTACA CTATGGAATT TGGATATTCT TCAAACAGTC CTTCCACTCA TAGGTCATAC AGCTACAGGC CATATAGCTA CCGGCACTTT GCACCCCCCA CCACACCCTG CAGCACAGAT GTTTGTGACA GTGACTATGC TCCTAGTCGG AGAATGACCT CAGTGGCAAC AGCCAAGGGC TATACCAGTG ACTTGAACTA TGATTCAGAA CCTGTGCCCC CACCTCCCAC ACCCCGAAGC CAATACTTGT CAGCAGAGGA GAACTATGAA AGCTGCCCAC CTTCTCCATA CACAGAGAGG AGCTATTCTC ATCACCTCTA CCCACCGCCA CCCTCTCCCT GTACAGACTC CTCCGGATCC GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT GACAAGTAGT GAGGGCCCGA TATCTCGACA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAGCTGAC GTCCTTTCCA TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCCT GGAAACGGGG GAGGCTAACT GAAACACGGA AGGAGACAAT ACCGGAAGGA ACCCGCGCTA TGACGGCAAT AAAAAGACAG AATAAAACGC ACGGGTGTTG GGTCGTTTGT TCATAAACGC GGGGTTCGGT CCCAGGGCTG GCACTCTGTC GATACCCCAC CGAGACCCCA TTGGGGCCAA TACGCCCGCG TTTCTTCCTT TTCCCCACCC CACCCCCCAA GTTCGGGTGA AGGCCCAGGG CTCGCAGCCA ACGTCGGGGC GGCAGGCCCT GCCATAGCAG ATCTGCGCAG CTGGGGCTCT AGGGGGTATC CCCACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG CATCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGGGGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTAATTCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCAAT GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCG ACGGATCGGG AGATCTCCCG ATCCCCTATG GTCGACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATC TGCTCCCTGC TTGTGTGTTG GAGGTCGCTG AGTAGTGCGC GAGCAAAATT TAAGCTACAA CAAGGCAAGG CTTGACCGAC AATTGCATGA AGAATCTGCT TAGG
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