pDawn-mCherry-Amp
$55.00
In stock
SKU
PL36080
100μg liquid plasmid(Instantly):$76
| name | pDawn-mCherry-Amp | another name | apDawn-mCherry-Amp |
| Insert Size (bp) | 861bp | Plasmid host | Escherichia coli |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | Red | |
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACAAAC ACCCCTTGTA TTACTGTTTA TGTAAGCAGA CAGTTTTATT GTTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGAGA TCCCGGTGCC TAATGAGTGA GCTAACTTAC ATCAATTGTC AATCGTTGAG CATGCCGGCG CGCATCGCGA GGCGAACCAG CTCCGAAAGG CTGTTGGCCT GCATCTTGGT CATGACGTTG GCCCGATACA CCTCGATGGT GCGCGGGCTG ATGTCGTACT CGCGGGCGAT CAGCTTGTTG GAAAGGCCGG CGATCAGCCC TTCCATGACC TGGCGCTCCC TGGGGCTCAA CGAGGCGACG CGGGCGGCGA TATCCTGCGC GACGGCCTCG CTCTTGGCGG CCGGCTCGGC CTGGCGGATC GCCGATTCGA TCATGGCGGT GAGGCGGTCG TCCTCGAAAG GCTTTTCCAG AAAGTCCACC GCCCCTAACT TCATCGCCTC GACCGCGAGC GGCACGTCGC CGTGACCGGT CATGATGAGG ATCGGAAAGG GGCTTTGCTG CGCCTTCATC CGCTTCAACA GCTCGATGCC GTCAAGGCCC GGCATGCGCA CGTCGGAGAC GACGCAGCCG AAGGAGAGAC CCGGCAGGGC GTCGAGAAAG GCTTGCGCGT CGTCAAACAG CGTGACGCCG AAGCCGGCAG AATCCAGCAG GAAATTCAGC GAATCCCGCA TCGCCGCGTC GTCGTCGATG ACGTAGATAT GTCCCTTGGT CGTCATGCTA GACCTCCTAT CATCTCGTCG GCTGCCGGGA GGGTGAAGCG GAAGGTCGCC CCGCCCGATG CGTTGCTCTC GGCCCACATG CGCCCGCCGT GAGCTTCGAT GATCGAGCGG CTGATGGACA GTCCCACGCC CATGCCGGTG TCCTTGGTGG TGAAGAAAGT CTGAAACAGG TTCGGAATGA CGTCGTCCTG GAAACCGCTG CCGGTGTCGG ACACTTCGAC CTCGATCATG TCGTCGGCGG CGGGGGTGTT GGTGACGACG AGCTCGCGTC GCTGCGACTG AGCCATCGCT TCCAGCGCGT TGCGGAACAG GTTGACCAGG ACCTGCTGGA TCTGCACCCG GTCGGCGAGA ACGAGATCGG CGCCCGGATC GAGACTGAAG CGGAGCTGCA CGTTCTGCTC GCGCGCGCCG GCAAGCCCGA GCGCGCCGGC CTCCTCGATC AGCTTGGAGA GACTCTCGAC CCGCTTCTCC GATTCGCCGC GGGCAACGAA GTCGCGCAGG CGCCGGATGA TCTGGCCGGC GCGCAGCGCC TGCTCGGCGG CGCGGTCCAG GGCGCTTTCG ACCTTCGGTG TGTTCGGATC ACTGCTGCCG GCAAGCAGCC GCCGCGAGCC CTTCATGTAG TTGCTGATCG CCGCCAGCGG CTGGTTGAGC TCGTGCGCGA GCGCGGACGC CATTTCGCCC ATGGCGCTCA GCCTGGAGAC GTGGACGAGC TCGGATTGCA GTTCCTGGAG GCGCGCCTGG GTCTGCTGGT GCTCGGTGAT ATCATTCTGA ATACCGACAA AATACGTTTT ATCCTCTATT TCCATTGGAT CAATATTTAA TTCATTCCAG AACATCGTTC CGTCTTTTTT GTAGTTTTGG ATCTGAACGG TGACCGGTTC TTTATTTTGT AAAGCGGTTC TGATGTTGTC CACTTCTGCA GGATCTGTGT GTTTCCCCTG TAAGAAGCGA CAGTTCTTTC CTAAAATTTC CTCGGTCTCG TAGCCGGTCA TTTGAACAAA GCCTTGATTT ACGTAGACAA TAGGATTATC TTCAAGTGCG GGATCTGTAA TTACCACACC GACTCGCACG TGATCAAGTG CTTTTTTGAT GACTTCCAGC TGTCCTGGTA TCCCAAATGA TTGAAAACTA GCCACATTCA CCACCCTCAA TTGACTCTCT TCCGGGCGCT ATCATGCCAT ACCGCGAAAG GTTTTGCACC ATTCGATGGT GTCCGGGATC TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTACG CCCGTGATCC TGATCACCGG CTATCCGGAC GAAAACATCT CGACCCGGGC CGCCGAGGCC GGCGTAAAAG ACGTGGTTTT GAAGCCGCTT CTCGACGAAA ACCTGCTCAA GCGTATCCGC CGCGCCATCC AGGACCGGCC TCGGGCATGA CCTACGGGGT TCTACGTAAG GCACCCCCCT TAAGATATCG CTCGAAATTT TCGAACCTCC CGATACCGCG TACCAATGCG TCATCACAAC GGAGTCTAGA AAAGAGGAGA AATACTAGAT GAGCACAAAA AAGAAACCAT TAACACAAGA GCAGCTTGAG GACGCACGTC GCCTTAAAGC AATTTATGAA AAAAAGAAAA ATGAACTTGG CTTATCCCAG GAATCTGTCG CAGACAAGAT GGGGATGGGG CAGTCAGGCG TTGGTGCTTT ATTTAATGGC ATCAATGCAT TAAATGCTTA TAACGCCGCA TTGCTTGCAA AAATTCTCAA AGTTAGCGTT GAAGAATTTA GCCCTTCAAT CGCCAGAGAA ATCTACGAGA TGTATGAAGC GGTTAGTATG CAGCCGTCAC TTAGAAGTGA GTATGAGTAC CCTGTTTTTT CTCATGTTCA GGCAGGGATG TTCTCACCTG AGCTTAGAAC CTTTACCAAA GGTGATGCGG AGAGATGGGT AAGCACAACC AAAAAAGCCA GTGATTCTGC ATTCTGGCTT GAGGTTGAAG GTAATTCCAT GACCGCACCA ACAGGCTCCA AGCCGAGCTT TCCTGACGGA ATGTTAATTC TCGTTGACCC TGAGCAGGCT GTTGAGCCAG GTGATTTCTG CATAGCCAGA CTTGGGGGTG ATGAGTTTAC CTTCAAGAAA CTGATCAGGG ATAGCGGTCA GGTGTTTTTA CAACCACTAA ACCCACAGTA CCCAATGATC CCATGCAATG AGAGTTGTTC CGTTGTGGGG AAAGTTATCG CTAGTCAGTG GCCTGAAGAG ACGTTTGGCG CTGCAAACGA CGAAAACTAC GCTTTAGTAG CTTAATAACG CTGATAGTGC TAGTGTAGAT CGCTACTAGA GCCAGGCATC AAATAAAACG AAAGGCTCAG TCGAAAGACT GGGCCTTTCG TTTTATCTGT TGTTTGTCGG TGAACGCTCT CTACTAGAGT CACACTGGCT CACCTTCGGG TGGGCCTTTC TGCGTTTATA TACTAGAGTA ACACCGTGCG TGTTGACTAT TTTACCTCTG GCGGTGATAA TGGTTGCTCT AGAAATAATT TTGTTTAACT TTAAGAAGGA GATATACCAT GGGCAGCAGC CATCATCATC ATCATCACAG CAGCGGCCTG GTGCCGCGCG GCAGCCATAT GGCTAGCATG ACTGGTGGAC AGCAAATGGG TCGGATCCAT ATGGTGAGCA AGGGCGAGGA GGATAACATG GCCATCATCA AGGAGTTCAT GCGCTTCAAG GTGCACATGG AGGGCTCCGT GAACGGCCAC GAGTTCGAGA TCGAGGGCGA GGGCGAGGGC CGCCCCTACG AGGGCACCCA GACCGCCAAG CTGAAGGTGA CCAAGGGTGG CCCCCTGCCC TTCGCCTGGG ACATCCTGTC CCCTCAGTTC ATGTACGGCT CCAAGGCCTA CGTGAAGCAC CCCGCCGACA TCCCCGACTA CTTGAAGCTG TCCTTCCCCG AGGGCTTCAA GTGGGAGCGC GTGATGAACT TCGAGGACGG CGGCGTGGTG ACCGTGACCC AGGACTCCTC CCTGCAGGAC GGCGAGTTCA TCTACAAGGT GAAGCTGCGC GGCACCAACT TCCCCTCCGA CGGCCCCGTA ATGCAGAAGA AGACCATGGG CTGGGAGGCC TCCTCCGAGC GGATGTACCC CGAGGACGGC GCCCTGAAGG GCGAGATCAA GCAGAGGCTG AAGCTGAAGG ACGGCGGCCA CTACGACGCT GAGGTCAAGA CCACCTACAA GGCCAAGAAG CCCGTGCAGC TGCCCGGCGC CTACAACGTC AACATCAAGT TGGACATCAC CTCCCACAAC GAGGACTACA CCATCGTGGA ACAGTACGAA CGCGCCGAGG GCCGCCACTC CACCGGCGGC ATGGACGAGC TGTACAAGTG AAAGCTTGCG GCCGCACTCG AGCACCACCA CCACCACCAC TGAGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGAT
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
