pLV3-CMV-Col5a1(mouse)-EF1a-EGFP
$128.00
In stock
SKU
PL36013
NM_015734.2
100μg liquid plasmid(Instantly):$149
| name | pLV3-CMV-Col5a1(mouse)-EF1a-EGFP | another name | apLV3-CMV-Col5a1(mouse)-EF1a-EGFP |
| Insert Size (bp) | 5517bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT AGAGCTAGCG AATTCGCCAC CATGGACGTA CACACCCGCT GGAAAGCTGC GCGCCCAGGC GCCCTGCTGC TGTCTTCGCC GCTACTCCTG TTCCTGCTGC TGCTGTGGGC GCCGCCTTCG AGCCGCGCAG CTCAGCCAGC AGATCTTCTG GAGATGCTAG ATTTTCACAA TTTGCCCTCA GGGGTAACGA AAACCACAGG TTTCTGTGCT ACTCGAAGAT CTTCCAGCGA GCCGGATGTT GCCTACCGAG TCTCTAAAGA TGCACAGCTC AGCATGCCCA CCAAGCAGCT GTACCCTGAG TCTGGTTTTC CCGAGGACTT CTCCATCCTG ACAACCGTGA AAGCCAAGAA AGGCAGCCAG GCCTTCCTAG TCTCCATTTA CAATGAGCAG GGCATCCAGC AGTTGGGGCT GGAGCTGGGC CGCTCCCCTG TCTTCCTCTA TGAGGACCAC ACAGGGAAGC CCGGGCCTGA AGAGTATCCG CTTTTCCCTG GCATCAACTT GTCCGATGGC AAGTGGCACC GAATTGCTCT CAGTGTCTAC AAGAAAAATG TCACCTTGAT CCTCGACTGT AAGAAGAAGA TTACGAAGTT CCTCAGCCGC AGTGACCACC CCATAATAGA CACCAATGGG ATTGTCATGT TTGGCTCCCG GATTCTGGAT GATGAAATAT TTGAGGGTGA CATCCAACAG TTGCTTTTCG TCTCTGACAA CCGAGCTGCC TATGACTACT GTGAGCACTA CAGCCCCGAC TGTGACACTG CGGTCCCTGA CACACCTCAG TCACAGGACC CTAACCCGGA TGAATATTAC CCAGAAGGAG AGGGTGAGAC CTATTACTAT GAGTATCCAT ATTATGAAGA CCCTGAAGAC CCGGGAAAGG AGCCTGCCCC TACTCAGAAG CCAGTGGAAG CTGCCAGAGA AACCACAGAG GTTCCTGAGG AGCAGACCCA GCCCCTACCC GAAGCCCCTA CAGTGCCTGA GACCAGTGAC ACGGCTGACA AGGAGGACAG TCTAGGGATC GGGGACTATG ACTACGTGCC CCCAGATGAC TATTACACTC CACCCCCATA TGAAGACTTT GGATATGGCG AGGGTGTGGA GAACCCTGAC CAGCCCACCA ACCCCGACTC AGGGGCTGAG GTCCCCACCA GCACCACTGT TACCTCCAAC ACCTCCAATC CAGCTCCAGG AGAAGGGAAG GATGACCTGG GCGGCGAATT CACCGAGGAA ACCATCAAGA ATCTAGAGGA AAACTACTAT GACCCGTACT TTGACCCCGA CTCCGACTCC AGTGTCTCTC CATCAGAGAT AGGGCCAGGC ATGCCCGCTA ACCAGGACAC CATCTTTGAG GGGATTGGAG GACCCCGAGG TGAGAAAGGG CAAAAGGGAG AACCAGCCAT CATTGAGCCG GGGATGCTGA TCGAGGGGCC CCCTGGCCCT GAAGGCCCTG CTGGTCTTCC AGGACCTCCA GGAACTACAG GTCCTACTGG CCAAATGGGT GACCCTGGAG AAAGGGGTCC CCCTGGGCGC CCAGGTCTTC CTGGAGCTGA TGGCTTGCCT GGCCCCCCAG GTACCATGCT CATGCTGCCG TTCCGGTTTG GAGGCGGTGG CGATGCCGGT TCTAAGGGCC CCATGGTCTC TGCGCAGGAG TCCCAGGCCC AGGCTATCCT CCAGCAAGCC AGGTTGGCAC TGAGGGGACC AGCTGGCCCA ATGGGTCTCA CCGGGAGACC TGGCCCCATG GGTCCTCCTG GGAGTGGCGG TTTGAAAGGT GAGCCAGGAG ACATGGGACC TCAGGGTCCT CGAGGTGTGC AAGGCCCACC TGGCCCAACA GGGAAGCCTG GAAGACGGGG CCGTGCTGGA AGTGATGGAG CCAGAGGCAT GCCTGGACAA ACAGGCCCCA AGGGTGACCG TGGCTTTGAT GGTCTGGCTG GGTTGCCGGG AGAGAAAGGC CATAGAGGTG ACCCTGGTCC TTCTGGCCCG CCCGGAATCC CAGGAGATGA TGGAGAAAGG GGTGACGATG GAGAAGTTGG GCCCAGGGGA CTGCCCGGGG AGCCTGGACC ACGTGGTCTG CTTGGGCCAA AAGGCCCCCC AGGGCCTCCT GGACCTCCTG GTGTAACGGG TATGGATGGC CAGCCTGGCC CAAAAGGAAA TGTGGGTCCC CAGGGAGAGC CTGGGCCGCC AGGACAGCAG GGTAATCCTG GTGCCCAGGG TCTTCCAGGT CCCCAGGGTG CCATTGGTCC TCCAGGAGAA AAGGGTCCTT TGGGGAAACC AGGTCTCCCA GGAATGCCAG GCGCTGATGG ACCCCCGGGG CACCCTGGAA AAGAAGGTCC TCCAGGAGAG AAAGGAGGCC AGGGTCCTCC TGGCCCCCAG GGTCCCATTG GCTACCCCGG TCCACGAGGA GTCAAGGGGG CAGATGGCAT CCGAGGTCTG AAGGGCACCA AGGGGGAGAA GGGTGAAGAC GGCTTCCCTG GGTTTAAAGG CGACATGGGA ATAAAGGGTG ACCGGGGGGA AATCGGCCCA CCTGGTCCCC GAGGAGAAGA TGGTCCTGAA GGTCCAAAGG GTCGAGGTGG TCCCAATGGT GATCCTGGTC CCCTGGGGCC CACTGGGGAA AAGGGAAAGC TTGGCGTGCC CGGATTACCG GGGTACCCAG GAAGACAAGG GCCAAAGGGT TCCATTGGAT TCCCTGGCTT CCCGGGCGCC AACGGAGAGA AGGGTGGCAG GGGGACACCT GGAAAGCCAG GACCACGGGG ACAGAGAGGC CCAACGGGCC CGCGGGGTGA ACGAGGCCCA CGAGGCATCA CGGGGAAGCC TGGCCCTAAG GGCAACTCCG GAGGTGATGG CCCAGCTGGC CCTCCTGGTG AACGGGGACC CAACGGACCC CAAGGTCCCA CCGGCTTTCC TGGACCCAAG GGTCCCCCGG GCCCACCAGG CAAGGACGGA CTCCCTGGAC ACCCTGGGCA GAGAGGGGAG ACCGGTTTCC AAGGCAAGAC TGGCCCTCCA GGGCCCCCAG GAGTGGTTGG CCCTCAGGGT CCCACAGGAG AGACGGGCCC CATGGGTGAG CGTGGCCATC CTGGTCCTCC AGGCCCTCCT GGTGAACAGG GCCTCCCAGG TGCTGCTGGG AAAGAAGGAA CGAAGGGTGA CCCAGGTCCT GCTGGCCTCC CTGGGAAGGA TGGCCCTCCA GGATTGCGTG GATTCCCTGG GGACCGAGGG CTACCTGGCC CCGTGGGAGC CCTTGGACTC AAAGGCAGTG AAGGCCCCCC TGGCCCACCA GGTCCTGCGG GTTCTCCAGG GGAGAGAGGA CCAGCTGGTG CCGCTGGGCC CATCGGAATT CCAGGGAGAC CTGGGCCTCA GGGACCTCCG GGGCCTGCTG GAGAGAAAGG ACTTCCTGGC GAGAAAGGTC CACAAGGCCC AGCTGGCCGA GATGGCCTCC AAGGTCCCGT GGGGCTCCCT GGACCAGCCG GCCCAGTGGG TCCTCCTGGA GAAGATGGAG ATAAGGGAGA GATCGGAGAG CCAGGGCAGA AGGGAAGCAA GGGCGACAAA GGCGAGCAGG GTCCTCCTGG GCCTACCGGT CCTCAAGGCC CGATTGGACA GCCAGGCCCT TCGGGAGCAG ATGGTGAACC TGGCCCTCGT GGACAGCAGG GCCTGTTTGG GCAGAAAGGA GATGAAGGTT CAAGAGGTTT CCCAGGACCC CCCGGGCCAG TGGGATTGCA GGGTTTGCCA GGACCTCCAG GAGAAAAGGG CGAGACAGGA GACGTGGGCC AGATGGGCCC TCCTGGTCCA CCAGGCCCCC GAGGACCCTC TGGAGCTCCA GGTGCCGATG GACCACAGGG TCCTCCTGGA GGGATTGGCA ACCCTGGTGC AGTCGGAGAA AAGGGAGAAC CTGGTGAAGC TGGAGATCCT GGCCTTCCAG GAGAAGGAGG TCCCCTGGGA CCTAAAGGAG AAAGAGGGGA GAAGGGAGAG GCTGGCCCCT CTGGTGCTGC TGGACCCCCT GGACCCAAAG GCCCTCCTGG AGATGATGGC CCCAAAGGCA GCCCTGGCCC TGTGGGCTTT CCTGGAGATC CTGGTCCCCC TGGAGAGCCA GGCCCCGCAG GTCAAGACGG CCCACCTGGT GACAAAGGGG ACGATGGTGA ACCTGGGCAG ACGGGGTCCC CGGGCCCTAC TGGTGAACCT GGTCCATCTG GGCCTCCAGG AAAGAGGGGT CCCCCAGGCC CTGCAGGCCC TGAAGGCAGG CAGGGGGAGA AAGGAGCCAA GGGAGAAGCT GGCTTAGAAG GCCCTCCTGG GAAGACTGGC CCCATTGGCC CCCAAGGGGC CCCTGGGAAG CCTGGCCCCG ATGGTCTCCG TGGAATCCCT GGTCCTGTGG GTGAGCAAGG CCTCCCAGGA TCCCCAGGCC CCGATGGTCC ACCCGGCCCC ATGGGTCCTC CAGGACTCCC TGGCCTCAAA GGAGACTCCG GTCCCAAAGG TGAAAAGGGC CATCCAGGCC TGATTGGACT CATCGGCCCT CCGGGAGAGC AAGGTGAAAA GGGTGACCGT GGACTCCCAG GCCCCCAGGG TTCATCTGGT CCTAAAGGAG ATCAGGGCAT CACAGGTCCT TCTGGCCCAC TTGGGCCTCC TGGTCCTCCT GGCTTGCCGG GCCCTCCAGG TCCCAAAGGT GCTAAGGGCT CTTCGGGTCC CACCGGCCCG AAGGGTGAGG CAGGCCACCC AGGACTCCCC GGCCCACCTG GCCCTCCGGG TGAGGTCATC CAGCCCCTGC CAATCCAGGC CTCCAGGACT CGGCGGAACA TTGATGCCAG CCAGCTCCTG GACGATGGGG CTGGGGAGAG CTACGTGGAT TATGCAGATG GCATGGAAGA GATCTTTGGT TCCCTCAACT CCCTGAAGCT GGAGATTGAA CAGATGAAGC GACCACTGGG CACCCAGCAG AACCCAGCCC GTACCTGCAA GGATCTACAG CTCTGTCATC CTGACTTCCC AGATGGCGAA TACTGGGTCG ATCCCAACCA AGGGTGCTCC AGGGACTCCT TCAAAGTCTA CTGCAATTTC ACAGCTGGAG GGTCCACGTG CGTCTTCCCT GACAAGAAGT CTGAGGGAGC CAGAATCACT TCTTGGCCCA AAGAAAACCC AGGTTCCTGG TTCAGTGAAT TCAAGCGTGG GAAACTGCTC TCCTATGTGG ATGCTGAAGG CAACCCCGTG GGCGTGGTAC AAATGACCTT CCTGCGGCTG CTGAGCGCCT CTGCCCACCA GAACGTCACC TACAACTGCT ACCAGTCCGT GGCCTGGCAG GATGCCGCCA CAGGCAGCTA TGATAAGGCT ATCCGCTTCT TGGGCTCCAA CGATGAGGAA ATGTCTTATG ATAACAACCC CTACATCCGT GCCCTGGTGG ATGGCTGTGC TACCAAGAAA GGCTACCAGA AGACGGTGCT GGAGATCGAC ACGCCCAAAG TAGAGCAAGT CCCCATTGTG GACATCATGT TCAACGACTT TGGCGAAGCC TCACAGAAAT TTGGATTTGA AGTGGGGCCA GCTTGCTTCC TAGGCTAGGC GGCCGCGAAG GATCTGCGAT CGCTCCGGTG CCCGTCAGTG GGCAGAGCGC ACATCGCCCA CAGTCCCCGA GAAGTTGGGG GGAGGGGTCG GCAATTGAAC GGGTGCCTAG AGAAGGTGGC GCGGGGTAAA CTGGGAAAGT GATGTCGTGT ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG GAGAACCGTA TATAAGTGCA GTAGTCGCCG TGAACGTTCT TTTTCGCAAC GGGTTTGCCG CCAGAACACA GCTGAAGCTT CGAGGGGCTC GCATCTCTCC TTCACGCGCC CGCCGCCCTA CCTGAGGCCG CCATCCACGC CGGTTGAGTC GCGTTCTGCC GCCTCCCGCC TGTGGTGCCT CCTGAACTGC GTCCGCCGTC TAGGTAAGTT TAAAGCTCAG GTCGAGACCG GGCCTTTGTC CGGCGCTCCC TTGGAGCCTA CCTAGACTCA GCCGGCTCTC CACGCTTTGC CTGACCCTGC TTGCTCAACT CTACGTCTTT GTTTCGTTTT CTGTTCTGCG CCGTTACAGA TCCAAGCTGT GACCGGCGCC TACGCTAGAT GACCGAGTAC AAGCCCACGG TGCGCCTCGC CACCCGCGAC GACGTCCCCC GGGACATGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCACGG CATGGACGAG CTGTACAAGT AGTCGACAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAATCATCGT CCTTTCCTTG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCCTGG TACCTTTAAG ACCAATGACT TACAAGGCAG CTGTAGATCT TAGCCACTTT TTAAAAGAAA AGGGGGGACT GGAAGGGCTA ATTCACTCCC AACGAAAATA AGATCTGCTT TTTGCTTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA GTCAGTGTGG AAAATCTCTA GCAGTAGTAG TTCATGTCAT CTTATTATTC AGTATTTATA ACTTGCAAAG AAATGAATAT CAGAGAGTGA GAGGAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGCTCT AGCTATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGACTTTTG CAGAGACGGC CCAAATTCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGCCAAG CTG
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