p35S-Fluc-MCS-GUS-Neo
$70.00
In stock
SKU
PL35821
100μg liquid plasmid(Instantly):$91
| name | p35S-Fluc-MCS-GUS-Neo | another name | ap35S-Fluc-MCS-GUS-Neo |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Luciferase Report | Fragment type | Promoter |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Neo/G418 | Fluorescent labeling | Fluc |
| Promoter | 35S | Replicon | oriV |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGAGCGTCGC AAAGGCGCTC GGTCTTGCCT TGCTCGTCGG TGATGTACTT CACCAGCTCC GCGAAGTCGC TCTTCTTGAT GGAGCGCATG GGGACGTGCT TGGCAATCAC GCGCACCCCC CGGCCGTTTT AGCGGCTAAA AAAGTCATGG CTCTGCCCTC GGGCGGACCA CGCCCATCAT GACCTTGCCA AGCTCGTCCT GCTTCTCTTC GATCTTCGCC AGCAGGGCGA GGATCGTGGC ATCACCGAAC CGCGCCGTGC GCGGGTCGTC GGTGAGCCAG AGTTTCAGCA GGCCGCCCAG GCGGCCCAGG TCGCCATTGA TGCGGGCCAG CTCGCGGACG TGCTCATAGT CCACGACGCC CGTGATTTTG TAGCCCTGGC CGACGGCCAG CAGGTAGGCC GACAGGCTCA TGCCGGCCGC CGCCGCCTTT TCCTCAATCG CTCTTCGTTC GTCTGGAAGG CAGTACACCT TGATAGGTGG GCTGCCCTTC CTGGTTGGCT TGGTTTCATC AGCCATCCGC TTGCCCTCAT CTGTTACGCC GGCGGTAGCC GGCCAGCCTC GCAGAGCAGG ATTCCCGTTG AGCACCGCCA GGTGCGAATA AGGGACAGTG AAGAAGGAAC ACCCGCTCGC GGGTGGGCCT ACTTCACCTA TCCTGCCCGG CTGACGCCGT TGGATACACC AAGGAAAGTC TACACGAACC CTTTGGCAAA ATCCTGTATA TCGTGCGAAA AAGGATGGAT ATACCGAAAA AATCGCTATA ATGACCCCGA AGCAGGGTTA TGCAGCGGAA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCAGA AGGCCGCCAG AGAGGCCGAG CGCGGCCGTG AGGCTTGGAC GCTAGGGCAG GGCATGAAAA AGCCCGTAGC GGGCTGCTAC GGGCGTCTGA CGCGGTGGAA AGGGGGAGGG GATGTTGTCT ACATGGCTCT GCTGTAGTGA GTGGGTTGCG CTCCGGCAGC GGTCCTGATC AATCGTCACC CTTTCTCGGT CCTTCAACGT TCCTGACAAC GAGCCTCCTT TTCGCCAATC CATCGACAAT CACCGCGAGT CCCTGCTCGA ACGCTGCGTC CGGACCGGCT TCGTCGAAGG CGTCTATCGC GGCCCGCAAC AGCGGCGAGA GCGGAGCCTG TTCAACGGTG CCGCCGCGCT CGCCGGCATC GCTGTCGCCG GCCTGCTCCT CAAGCACGGC CCCAACAGTG AAGTAGCTGA TTGTCATCAG CGCATTGACG GCGTCCCCGG CCGAAAAACC CGCCTCGCAG AGGAAGCGAA GCTGCGCGTC GGCCGTTTCC ATCTGCGGTG CGCCCGGTCG CGTGCCGGCA TGGATGCGCG CGCCATCGCG GTAGGCGAGC AGCGCCTGCC TGAAGCTGCG GGCATTCCCG ATCAGAAATG AGCGCCAGTC GTCGTCGGCT CTCGGCACCG AATGCGTATG ATTCTCCGCC AGCATGGCTT CGGCCAGTGC GTCGAGCAGC GCCCGCTTGT TCCTGAAGTG CCAGTAAAGC GCCGGCTGCT GAACCCCCAA CCGTTCCGCC AGTTTGCGTG TCGTCAGACC GTCTACGCCG ACCTCGTTCA ACAGGTCCAG GGCGGCACGG ATCACTGTAT TCGGCTGCAA CTTTGTCATG CTTGACACTT TATCACTGAT AAACATAATA TGTCCACCAA CTTATCAGTG ATAAAGAATC CGCGCGTTCA ATCGGACCAG CGGAGGCTGG TCCGGAGGCC AGACGTGAAA CCCAACATAC CCCTGATCGT AATTCTGAGC ACTGTCGCGC TCGACGCTGT CGGCATCGGC CTGATTATGC CGGTGCTGCC GGGCCTCCTG CGCGATCTGG TTCACTCGAA CGACGTCACC GCCCACTATG GCATTCTGCT GGCGCTGTAT GCGTTGGTGC AATTTGCCTG CGCACCTGTG CTGGGCGCGC TGTCGGATCG TTTCGGGCGG CGGCCAATCT TGCTCGTCTC GCTGGCCGGC GCCAGATCTG GGGAACCCTG TGGTTGGCAT GCACATACAA ATGGACGAAC GGATAAACCT TTTCACGCCC TTTTAAATAT CCGATTATTC TAATAAACGC TCTTTTCTCT TAGGTTTACC CGCCAATATA TCCTGTCAAA CACTGATAGT TTAAACTGAA GGCGGGAAAC GACAATCTGA TCATGAGCGG AGAATTAAGG GAGTCACGTT ATGACCCCCG CCGATGACGC GGGACAAGCC GTTTTACGTT TGGAACTGAC AGAACCGCAA CGTTGAAGGA GCCACTCAGC CGCGGGTTTC TGGAGTTTAA TGAGCTAAGC ACATACGTCA GAAACCATTA TTGCGCGTTC AAAAGTCGCC TAAGGTCACT ATCAGCTAGC AAATATTTCT TGTCAAAAAT GCTCCACTGA CGTTCCATAA ATTCCCCTCG GTATCCAATT AGAGTCTCAT ATTCACTCTC AATCCAAATA ATCTGCACCG GATCTGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GATGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACGGGATCTC TGCGGAACAG GCGGTCGAAG GTGCCGATAT CATTACGACA GCAACGGCCG ACAAGCACAA CGCCACGATC CTGAGCGACA ATATGATCGG GCCCGGCGTC CACATCAACG GCGTCGGCGG CGACTGCCCA GGCAAGACCG AGATGCACCG CGATATCTTG CTGCGTTCGG ATATTTTCGT GGAGTTCCCG CCACAGACCC GGATGATCCC CGATCGTTCA AACATTTGGC AATAAAGTTT CTTAAGATTG AATCCTGTTG CCGGTCTTGC GATGATTATC ATATAATTTC TGTTGAATTA CGTTAAGCAT GTAATAATTA ACATGTAATG CATGACGTTA TTTATGAGAT GGGTTTTTAT GATTAGAGTC CCGCAATTAT ACATTTAATA CGCGATAGAA AACAAAATAT AGCGCGCAAA CTAGGATAAA TTATCGCGCG CGGTGTCATC TATGTTACTA GATCGGGCCT CCTGTCAATG CTGGCGGCGG CTCTGGTGGT GGTTCTGGTG GCGGCTCTGA GGGTGGTGGC TCTGAGGGTG GCGGTTCTGA GGGTGGCGGC TCTGAGGGAG GCGGTTCCGG TGGTGGCTCT GGTTCCGGTG ATTTTGATTA TGAAAAGATG GCAAACGCTA ATAAGGGGGC TATGACCGAA AATGCCGATG AAAACGCGCT ACAGTCTGAC GCTAAAGGCA AACTTGATTC TGTCGCTACT GATTACGGTG CTGCTATCGA TGGTTTCATT GGTGACGTTT CCGGCCTTGC TAATGGTAAT GGTGCTACTG GTGATTTTGC TGGCTCTAAT TCCCAAATGG CTCAAGTCGG TGACGGTGAT AATTCACCTT TAATGAATAA TTTCCGTCAA TATTTACCTT CCCTCCCTCA ATCGGTTGAA TGTCGCCCTT TTGTCTTTGG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTTCAGAA GACCAAAGGG CTATTGAGAC TTTTCAACAA AGGGTAATTT CGGGAAACCT CCTCGGATTC CATTGCCCAG CTATCTGTCA CTTCATCGAA AGGACAGTAG AAAAGGAAGG TGGCTCCTAC AAATGCCATC ATTGCGATAA AGGAAAGGCT ATCATTCAAG ATGCCTCTGC CGACAGTGGT CCCAAAGATG GACCCCCACC CACGAGGAGC ATCGTGGAAA AAGAAGACGT TCCAACCACG TCTTCAAAGC AAGTGGATTG ATGTGACATC TCCACTGACG TAAGGGATGA CGCACAATCC CACTATCCTT CGCAAGACCC TTCCTCTATA TAAGGAAGTT CATTTCATTT GGAGAGGACA GCGGTGGAGA TCGAATTCCA TGGAAGACGC CAAAAACATA AAGAAAGGCC CGGCGCCATT CTATCCGCTG GAAGATGGAA CCGCTGGAGA GCAACTGCAT AAGGCTATGA AGAGATACGC CCTGGTTCCT GGAACAATTG CTTTTACAGA TGCACATATC GAGGTGGACA TCACTTACGC TGAGTACTTC GAAATGTCCG TTCGGTTGGC AGAAGCTATG AAACGATATG GGCTGAATAC AAATCACAGA ATCGTCGTAT GCAGTGAAAA CTCTCTTCAA TTCTTTATGC CGGTGTTGGG CGCGTTATTT ATCGGAGTTG CAGTTGCGCC CGCGAACGAC ATTTATAATG AACGTGAATT GCTCAACAGT ATGGGCATTT CGCAGCCTAC CGTGGTGTTC GTTTCCAAAA AGGGGTTGCA AAAAATTTTG AACGTGCAAA AAAAGCTCCC AATCATCCAA AAAATTATTA TCATGGATTC TAAAACGGAT TACCAGGGAT TTCAGTCGAT GTACACGTTC GTCACATCTC ATCTACCTCC CGGTTTTAAT GAATACGATT TTGTGCCAGA GTCCTTCGAT AGGGACAAGA CAATTGCACT GATCATGAAC TCCTCTGGAT CTACTGGTCT GCCTAAAGGT GTCGCTCTGC CTCATAGAAC TGCCTGCGTG AGATTCTCGC ATGCCAGAGA TCCTATTTTT GGCAATCAAA TCATTCCGGA TACTGCGATT TTAAGTGTTG TTCCATTCCA TCACGGTTTT GGAATGTTTA CTACACTCGG ATATTTGATA TGTGGATTTC GAGTCGTCTT AATGTATAGA TTTGAAGAAG AGCTGTTTCT GAGGAGCCTT CAGGATTACA AGATTCAAAG TGCGCTGCTG GTGCCAACCC TATTCTCCTT CTTCGCCAAA AGCACTCTGA TTGACAAATA CGATTTATCT AATTTACACG AAATTGCTTC TGGTGGCGCT CCCCTCTCTA AGGAAGTCGG GGAAGCGGTT GCCAAGAGGT TCCATCTGCC AGGTATCAGG CAAGGATATG GGCTCACTGA GACTACATCA GCTATTCTGA TTACACCCGA GGGGGATGAT AAACCGGGCG CGGTCGGTAA AGTTGTTCCA TTTTTTGAAG CGAAGGTTGT GGATCTGGAT ACCGGGAAAA CGCTGGGCGT TAATCAAAGA GGCGAACTGT GTGTGAGAGG TCCTATGATT ATGTCCGGTT ATGTAAACAA TCCGGAAGCG ACCAACGCCT TGATTGACAA GGATGGATGG CTACATTCTG GAGACATAGC TTACTGGGAC GAAGACGAAC ACTTCTTCAT CGTTGACCGC CTGAAGTCTC TGATTAAGTA CAAAGGCTAT CAGGTGGCTC CCGCTGAATT GGAATCCATC TTGCTCCAAC ACCCCAACAT CTTCGACGCA GGTGTCGCAG GTCTTCCCGA CGATGACGCC GGTGAACTTC CCGCCGCCGT TGTTGTTTTG GAGCACGGAA AGACGATGAC GGAAAAAGAG ATCGTGGATT ACGTCGCCAG TCAAGTAACA ACCGCGAAAA AGTTGCGCGG AGGAGTTGTG TTTGTGGACG AAGTACCGAA AGGTCTTACC GGAAAACTCG ACGCAAGAAA AATCAGAGAG ATCCTCATAA AGGCCAAGAA GGGCGGAAAG ATCGCCGTGT AATTCTAGAG AATTCGCTGA AATCACCAGT CTCTCTCTAC AAATCTATCT CTCTCTATTT TCTCCATAAA TAATGTGTGA GTAGTTTCCC GATAAGGGAA ATTAGGGTTC TTATAGGGTT TCGCTCATGT GTTGAGCATA TAAGAAACCC TTAGTATGTA TTTGTATTTG TAAAATACTT CTATCAATAA AATTTCTAAT TCCTAAAACC AAAATCCAGT ACTAAAATCC AGATCCACTA GCCTCCTCGA GGTCGAGTCG ACTCTAGAGG ATCCCCGGGT GGTCAGTCCC TTATGTTACG TCCTGTAGAA ACCCCAACCC GTGAAATCAA AAAACTCGAC GGCCTGTGGG CATTCAGTCT GGATCGCGAA AACTGTGGAA TTGATCAGCG TTGGTGGGAA AGCGCGTTAC AAGAAAGCCG GGCAATTGCT GTGCCAGGCA GTTTTAACGA TCAGTTCGCC GATGCAGATA TTCGTAATTA TGCGGGCAAC GTCTGGTATC AGCGCGAAGT CTTTATACCG AAAGGTTGGG CAGGCCAGCG TATCGTGCTG CGTTTCGATG CGGTCACTCA TTACGGCAAA GTGTGGGTCA ATAATCAGGA AGTGATGGAG CATCAGGGCG GCTATACGCC ATTTGAAGCC GATGTCACGC CGTATGTTAT TGCCGGGAAA AGTGTACGTA TCACCGTTTG TGTGAACAAC GAACTGAACT GGCAGACTAT CCCGCCGGGA ATGGTGATTA CCGACGAAAA CGGCAAGAAA AAGCAGTCTT ACTTCCATGA TTTCTTTAAC TATGCCGGAA TCCATCGCAG CGTAATGCTC TACACCACGC CGAACACCTG GGTGGACGAT ATCACCGTGG TGACGCATGT CGCGCAAGAC TGTAACCACG CGTCTGTTGA CTGGCAGGTG GTGGCCAATG GTGATGTCAG CGTTGAACTG CGTGATGCGG ATCAACAGGT GGTTGCAACT GGACAAGGCA CTAGCGGGAC TTTGCAAGTG GTGAATCCGC ACCTCTGGCA ACCGGGTGAA GGTTATCTCT ATGAACTGTG CGTCACAGCC AAAAGCCAGA CAGAGTGTGA TATCTACCCG CTTCGCGTCG GCATCCGGTC AGTGGCAGTG AAGGGCGAAC AGTTCCTGAT TAACCACAAA CCGTTCTACT TTACTGGCTT TGGTCGTCAT GAAGATGCGG ACTTGCGTGG CAAAGGATTC GATAACGTGC TGATGGTGCA CGACCACGCA TTAATGGACT GGATTGGGGC CAACTCCTAC CGTACCTCGC ATTACCCTTA CGCTGAAGAG ATGCTCGACT GGGCAGATGA ACATGGCATC GTGGTGATTG ATGAAACTGC TGCTGTCGGC TTTAACCTCT CTTTAGGCAT TGGTTTCGAA GCGGGCAACA AGCCGAAAGA ACTGTACAGC GAAGAGGCAG TCAACGGGGA AACTCAGCAA GCGCACTTAC AGGCGATTAA AGAGCTGATA GCGCGTGACA AAAACCACCC AAGCGTGGTG ATGTGGAGTA TTGCCAACGA ACCGGATACC CGTCCGCAAG GTGCACGGGA ATATTTCGCG CCACTGGCGG AAGCAACGCG TAAACTCGAC CCGACGCGTC CGATCACCTG CGTCAATGTA ATGTTCTGCG ACGCTCACAC CGATACCATC AGCGATCTCT TTGATGTGCT GTGCCTGAAC CGTTATTACG GATGGTATGT CCAAAGCGGC GATTTGGAAA CGGCAGAGAA GGTACTGGAA AAAGAACTTC TGGCCTGGCA GGAGAAACTG CATCAGCCGA TTATCATCAC CGAATACGGC GTGGATACGT TAGCCGGGCT GCACTCAATG TACACCGACA TGTGGAGTGA AGAGTATCAG TGTGCATGGC TGGATATGTA TCACCGCGTC TTTGATCGCG TCAGCGCCGT CGTCGGTGAA CAGGTATGGA ATTTCGCCGA TTTTGCGACC TCGCAAGGCA TATTGCGCGT TGGCGGTAAC AAGAAAGGGA TCTTCACTCG CGACCGCAAA CCGAAGTCGG CGGCTTTTCT GCTGCAAAAA CGCTGGACTG GCATGAACTT CGGTGAAAAA CCGCAGCAGG GAGGCAAACA ATGAATCAAC AACTCTCCTG GCGCACCATC GTCGGCTACA GCCTCGGGAA TTGCTACCGA GCTCGAATTT CCCCGATCGT TCAAACATTT GGCAATAAAG TTTCTTAAGA TTGAATCCTG TTGCCGGTCT TGCGATGATT ATCATATAAT TTCTGTTGAA TTACGTTAAG CATGTAATAA TTAACATGTA ATGCATGACG TTATTTATGA GATGGGTTTT TATGATTAGA GTCCCGCAAT TATACATTTA ATACGCGATA GAAAACAAAA TATAGCGCGC AAACTAGGAT AAATTATCGC GCGCGGTGTC ATCTATGTTA CTAGATCGGG AATTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT TGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGGCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGAACCA CCATCAAACA GGATTTTCGC CTGCTGGGGC AAACCAGCGT GGACCGCTTG CTGCAACTCT CTCAGGGCCA GGCGGTGAAG GGCAATCAGC TGTTGCCCGT CTCACTGGTG AAAAGAAAAA CCACCCCAGT ACATTAAAAA CGTCCGCAAT GTGTTATTAA GTTGTCTAAG CGTCAATTTG TTTACACCAC AATATATCCT GCCACCAGCC AGCCAACAGC TCCCCGACCG GCAGCTCGGC ACAAAATCAC CACTCGATAC AGGCAGCCCA TCAGTCCGGG ACGGCGTCAG CGGGAGAGCC GTTGTAAGGC GGCAGACTTT GCTCATGTTA CCGATGCTAT TCGGAAGAAC GGCAACTAAG CTGCCGGGTT TGAAACACGG ATGATCTCGC GGAGGGTAGC ATGTTGATTG TAACGATGAC AGAGCGTTGC TGCCTGTGAT CAAATATCAT CTCCCTCGCA GAGATCCGAA TTATCAGCCT TCTTATTCAT TTCTCGCTTA ACCGTGACAG GCTGTCGATC TTGAGAACTA TGCCGACATA ATAGGAAATC GCTGGATAAA GCCGCTGAGG AAGCTGAGTG GCGCTATTTC TTTAGAAGTG AACGTTGACG ATATCAACTC CCCTATCCAT TGCTCACCGA ATGGTACAGG TCGGGGACCC GAAGTTCCGA CTGTCGGCCT GATGCATCCC CGGCTGATCG ACCCCAGATC TGGGGCTGAG AAAGCCCAGT AAGGAAACAA CTGTAGGTTC GAGTCGCGAG ATCCCCCGGA ACCAAAGGAA GTAGGTTAAA CCCGCTCCGA TCAGGCCGAG CCACGCCAGG CCGAGAACAT TGGTTCCTGT AGGCATCGGG ATTGGCGGAT CAAACACTAA AGCTACTGGA ACGAGCAGAA GTCCTCCGGC CGCCAGTTGC CAGGCGGTAA AGGTGAGCAG AGGCACGGGA GGTTGCCACT TGCGGGTCAG CACGGTTCCG AACGCCATGG AAACCGCCCC CGCCAGGCCC GCTGCGACGC CGACAGGATC TAGCGCTGCG TTTGGTGTCA ACACCAACAG CGCCACGCCC GCAGTTCCGC AAATAGCCCC CAGGACCGCC ATCAATCGTA TCGGGCTACC TAGCAGAGCG GCAGAGATGA ACACGACCAT CAGCGGCTGC ACAGCGCCTA CCGTCGCCGC GACCCCGCCC GGCAGGCGGT AGACCGAAAT AAACAACAAG CTCCAGAATA GCGAAATATT AAGTGCGCCG AGGATGAAGA TGCGCATCCA CCAGATTCCC GTTGGAATCT GTCGGACGAT CATCACGAGC AATAAACCCG CCGGCAACGC CCGCAGCAGC ATACCGGCGA CCCCTCGGCC TCGCTGTTCG GGCTCCACGA AAACGCCGGA CAGATGCGCC TTGTGAGCGT CCTTGGGGCC GTCCTCCTGT TTGAAGACCG ACAGCCCAAT GATCTCGCCG TCGATGTAGG CGCCGAATGC CACGGCATCT CGCAACCGTT CAGCGAACGC CTCCATGGGC TTTTTCTCCT CGTGCTCGTA AACGGACCCG AACATCTCTG GAGCTTTCTT CAGGGCCGAC AATCGGATCT CGCGGAAATC CTGCACGTCG GCCGCTCCAA GCCGTCGAAT CTGAGCCTTA ATCACAATTG TCAATTTTAA TCCTCTGTTT ATCGGCAGTT CGTAGAGCGC GCCGTGCGTC CCGAGCGATA CTGAGCGAAG CAAGTGCGTC GAGCAGTGCC CGCTTGTTCC TGAAATGCCA GTAAAGCGCT GGCTGCTGAA CCCCCAGCCG GAACTGACCC CACAAGGCCC TAGCGTTTGC AATGCACCAG GTCATCATTG ACCCAGGCGT GTTCCACCAG GCCGCTGCCT CGCAACTCTT CGCAGGCTTC GCCGACCTGC TCGCGCCACT TCTTCACGCG GGTGGAATCC GATCCGCACA TGAGGCGGAA GGTTTCCAGC TTGAGCGGGT ACGGCTCCCG GTGCGAGCTG AAATAGTCGA ACATCCGTCG GGCCGTCGGC GACAGCTTGC GGTACTTCTC CCATATGAAT TTCGTGTAGT GGTCGCCAGC AAACAGCACG ACGATTTCCT CGTCGATCAG GACCTGGCAA CGGGACGTTT TCTTGCCACG GTCCAGGACG CGGAAGCGGT GCAGCAGCGA CACCGATTCC AGGTGCCCAA CGCGGTCGGA CGTGAAGCCC ATCGCCGTCG CCTGTAGGCG CGACAGGCAT TCCTCGGCCT TCGTGTAATA CCGGCCATTG ATCGACCAGC CCAGGTCCTG GCAAAGCTCG TAGAACGTGA AGGTGATCGG CTCGCCGATA GGGGTGCGCT TCGCGTACTC CAACACCTGC TGCCACACCA GTTCGTCATC GTCGGCCCGC AGCTCGACGC CGGTGTAGGT GATCTTCACG TCCTTGTTGA CGTGGAAAAT GACCTTGTTT TGCAGCGCCT CGCGCGGGAT TTTCTTGTTG CGCGTGGTGA ACAGGGCAGA GCGGGCCGTG TCGTTTGGCA TCGCTCGCAT CGTGTCCGGC CACGGCGCAA TATCGAACAA GGAAAGCTGC ATTTCCTTGA TCTGCTGCTT CGTGTGTTTC AGCAACGCGG CCTGCTTGGC CTCGCTGACC TGTTTTGCCA GGTCCTCGCC GGCGGTTTTT CGCTTCTTGG TCGTCATAGT TCCTCGCGTG TCGATGGTCA TCGACTTCGC CAAACCTGCC GCCTCCTGTT CGAGACGACG CGAACGCTCC ACGGCGGCCG ATGGCGCGGG CAGGGCAGGG GGAGCCAGTT GCACGCTGTC GCGCTCGATC TTGGCCGTAG CTTGCTGGAC CATCGAGCCG ACGGACTGGA AGGTTTCGCG GGGCGCACGC ATGACGGTGC GGCTTGCGAT GGTTTCGGCA TCCTCGGCGG AAAACCCCGC GTCGATCAGT TCTTGCCTGT ATGCCTTCCG GTCAAACGTC CGATTCATTC ACCCTCCTTG CGGGATTGCC CCGACTCACG CCGGGGCAAT GTGCCCTTAT TCCTGATTTG ACCCGCCTGG TGCCTTGGTG TCCAGATAAT CCACCTTATC GGCAATGAAG TCGGTCCCGT AGACCGTCTG GCCGTCCTTC TCGTACTTGG TATTCCGAAT CTTGCCCTGC ACGAATACCA GCGACCCCTT GCCCAAATAC TTGCCGTGGG CCTCGGCCTG AGAGCCAAAA CACTTGATGC GGAAGAAGTC GGTGCGCTCC TGCTTGTCGC CGGCATCGTT GCGCCACATC TAGGTACTAA AACAATTCAT CCAGTAAAAT ATAATATTTT ATTTTCTCCC AATCAGGCTT GATCCCCAGT AAGTCAAAAA ATAGCTCGAC ATACTGTTCT TCCCCGATAT CCTCCCTGAT CGACCGGACG CAGAAGGCAA TGTCATACCA CTTGTCCGCC CTGCCGCTTC TCCCAAGATC AATAAAGCCA CTTACTTTGC CATCTTTCAC AAAGATGTTG CTGTCTCCCA GGTCGCCGTG GGAAAAGACA AGTTCCTCTT CGGGCTTTTC CGTCTTTAAA AAATCATACA GCTCGCGCGG ATCTTTAAAT GGAGTGTCTT CTTCCCAGTT TTCGCAATCC ACATCGGCCA GATCGTTATT CAGTAAGTAA TCCAATTCGG CTAAGCGGCT GTCTAAGCTA TTCGTATAGG GACAATCCGA TATGTCGATG GAGTGAAAGA GCCTGATGCA CTCCGCATAC AGCTCGATAA TCTTTTCAGG GCTTTGTTCA TCTTCATACT CTTCCGAGCA AAGGACGCCA TCGGCCTCAC TCATGAGCAG ATTGCTCCAG CCATCATGCC GTTCAAAGTG CAGGACCTTT GGAACAGGCA GCTTTCCTTC CAGCCATAGC ATCATGTCCT TTTCCCGTTC CACATCATAG GTGGTCCCTT TATACCGGCT GTCCGTCATT TTTAAATATA GGTTTTCATT TTCTCCCACC AGCTTATATA CCTTAGCAGG AGACATTCCT TCCGTATCTT TTACGCAGCG GTATTTTTCG ATCAGTTTTT TCAATTCCGG TGATATTCTC ATTTTAGCCA TTTATTATTT CCTTCCTCTT TTCTACAGTA TTTAAAGATA CCCCAAGAAG CTAATTATAA CAAGACGAAC TCCAATTCAC TGTTCCTTGC ATTCTAAAAC CTTAAATACC AGAAAACAGC TTTTTCAAAG TTGTTTTCAA AGTTGGCGTA TAACATAGTA TCGACGGAGC CGATTTTGAA ACCACAATTA TGGGTGATGC TGCCAACTTA CTGATTTAGT GTATGATGGT GTTTTTGAGG TGCTCCAGTG GCTTCTGTGT CTATCAGCTG TCCCTCCTGT TCAGCTACTG ACGGGGTGGT GCGTAACGGC AAAAGCACCG CCGGACATCA GCGCTATCTC TGCTCTCACT GCCGTAAAAC ATGGCAACTG CAGTTCACTT ACACCGCTTC TCAACCCGGT ACGCACCAGA AAATCATTGA TATGGCCATG AATGGCGTTG GATGCCGGGC AACAGCCCGC ATTATGGGCG TTGGCCTCAA CACGATTTTA CGTCACTTAA AAAACTCAGG CCGCAGTCGG TAACCTCGCG CATACAGCCG GGCAGTGACG TCATCGTCTG CGCGGAAATG GACGAACAGT GGGGCTATGT CGGGGCTAAA TCGCGCCAGC GCTGGCTGTT TTACGCGTAT GACAGTCTCC GGAAGACGGT TGTTGCGCAC GTATTCGGTG AACGCACTAT GGCGACGCTG GGGCGTCTTA TGAGCCTGCT GTCACCCTTT GACGTGGTGA TATGGATGAC GGATGGCTGG CCGCTGTATG AATCCCGCCT GAAGGGAAAG CTGCACGTAA TCAGCAAGCG ATATACGCAG CGAATTGAGC GGCATAACCT GAATCTGAGG CAGCACCTGG CACGGCTGGG ACGGAAGTCG CTGTCGTTCT CAAAATCGGT GGAGCTGCAT GACAAAGTCA TCGGGCATTA TCTGAACATA AAACACTATC AATAAGTTGG AGTCATTACC CAATTATGAT AGAATTTACA AGCTATAAGG TTATTGTCCT GGGTTTCAAG CATTAGTCCA TGCAAGTTTT TATGCTTTGC CCATTCTATA GATATATTGA TAAGCGCGCT GCCTATGCCT TGCCCCCTGA AATCCTTACA TACGGCGATA TCTTCTATAT AAAAGATATA TTATCTTATC AGTATTGTCA ATATATTCAA GGCAATCTGC CTCCTCATCC TCTTCATCCT CTTCGTCTTG GTAGCTTTTT AAATATGGCG CTTCATAGAG TAATTCTGTA AAGGTCCAAT TCTCGTTTTC ATACCTCGGT ATAATCTTAC CTATCACCTC AAATGGTTCG CTGGGTTTAT CGCACCCCCG AACACGAGCA CGGCACCCGC GACCACTATG CCAAGAATGC CCAAGGTAAA AATTGCCGGC CCCGCCATGA AGTCCGTGAA TGCCCCGACG GCCGAAGTGA AGGGCAGGCC GCCACCCAGG CCGCCGCCCT CACTGCCCGG CACCTGGTCG CTGAATGTCG ATGCCAGCAC CTGCGGCACG TCAATGCTTC CGGGCGTCGC GCTCGGGCTG ATCGCCCATC CCGTTACTGC CCCGATCCCG GCAATGGCAA GGACTGCCAG CGCTGCCATT TTTGGGGTGA GGCCGTTCGC GGCCGAGGGG CGCAGCCCCT GGGGGGATGG GAGGCCCGCG TTAGCGGGCC GGGAGGGTTC GAGAAGGGGG GGCACCCCCC TTCGGCGTGC GCGGTCACGC GCACAGGGCG CAGCCCTGGT TAAAAACAAG GTTTATAAAT ATTGGTTTAA AAGCAGGTTA AAAGACAGGT TAGCGGTGGC CGAAAAACGG GCGGAAACCC TTGCAAATGC TGGATTTTCT GCCTGTGGAC AGCCCCTCAA ATGTCAATAG GTGCGCCCCT CATCTGTCAG CACTCTGCCC CTCAAGTGTC AAGGATCGCG CCCCTCATCT GTCAGTAGTC GCGCCCCTCA AGTGTCAATA CCGCAGGGCA CTTATCCCCA GGCTTGTCCA CATCATCTGT GGGAAACTCG CGTAAAATCA GGCGTTTTCG CCGATTTGCG AGGCTGGCCA GCTCCACGTC GCCGGCCGAA ATCGAGCCTG CCCCTCATCT GTCAACGCCG CGCCGGGTGA GTCGGCCCCT CAAGTGTCAA CGTCCGCCCC TCATCTGTCA GTGAGGGCCA AGTTTTCCGC GAGGTATCCA CAACGCCGGC GGCCGCGGTG TCTCGCACAC GGCTTCGACG GCGTTTCTGG CGCGTTTGCA GGGCCATAGA CGGCCGCCAG CCCAGCGGCG AGGGCAACCA GCCCGG
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