NM_004774.4;CRSP1; CRSP200; DRIP205; DRIP230; PBP;PPARBP; PPARGBP; RB18A; TRAP220; TRIP2
100μg liquid plasmid(Instantly):$149
| name | pTRE3G-MED1(human)-V5-TurboID-TetOne-Puro | another name | apTRE3G-MED1(human)-V5-TurboID-TetOne-Puro |
| Insert Size (bp) | 4743bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression,Tetracycline induction | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | TRE3G | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTCGAGTTTA CTCCCTATCA GTGATAGAGA ACGTATGAAG AGTTTACTCC CTATCAGTGA TAGAGAACGT ATGCAGACTT TACTCCCTAT CAGTGATAGA GAACGTATAA GGAGTTTACT CCCTATCAGT GATAGAGAAC GTATGACCAG TTTACTCCCT ATCAGTGATA GAGAACGTAT CTACAGTTTA CTCCCTATCA GTGATAGAGA ACGTATATCC AGTTTACTCC CTATCAGTGA TAGAGAACGT ATAAGCTTTA GGCGTGTACG GTGGGCGCCT ATAAAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGCAATTCC ACAACACTTT TGTCTTATAC CAACTTTCCG TACCACTTCC TACCCTCGTA AAGTCGACAC CGGGGCCCAG ATCTATCGAT CGGCCGGATA TCACGCGTCA TATGGCTAGC CTGCAGGGAT CCGGTAGATC GGGTGAATTC GCCACCATGA AAGCTCAGGG GGAAACCGAG GAGTCAGAAA AGCTGAGTAA GATGAGTTCT CTCCTGGAAC GGCTCCATGC AAAATTTAAC CAAAATAGAC CCTGGAGTGA AACCATTAAG CTTGTGCGTC AAGTCATGGA GAAGAGGGTT GTGATGAGTT CTGGAGGGCA TCAACATTTG GTCAGCTGTT TGGAGACATT GCAGAAGGCT CTCAAAGTAA CATCTTTACC AGCAATGACT GATCGTTTGG AGTCCATAGC AAGACAGAAT GGACTGGGCT CTCATCTCAG TGCCAGTGGC ACTGAATGTT ACATCACGTC AGATATGTTC TATGTGGAAG TGCAGTTAGA TCCTGCAGGA CAGCTTTGTG ATGTAAAAGT GGCTCACCAT GGGGAGAATC CTGTGAGCTG TCCGGAGCTT GTACAGCAGC TAAGGGAAAA AAATTTTGAT GAATTTTCTA AGCACCTTAA GGGCCTTGTT AATCTGTATA ACCTTCCAGG GGACAACAAA CTGAAGACTA AAATGTACTT GGCTCTCCAA TCCTTAGAAC AAGATCTTTC TAAAATGGCA ATTATGTACT GGAAAGCAAC TAATGCTGGT CCCTTGGATA AGATTCTTCA TGGAAGTGTT GGCTATCTCA CACCAAGGAG TGGGGGTCAT TTAATGAACC TGAAGTACTA TGTCTCTCCT TCTGACCTAC TGGATGACAA GACTGCATCT CCCATCATTT TGCATGAGAA TAATGTTTCT CGATCTTTGG GCATGAATGC ATCAGTGACA ATTGAAGGAA CATCTGCTGT GTACAAACTC CCAATTGCAC CATTAATTAT GGGGTCACAT CCAGTTGACA ATAAATGGAC CCCTTCCTTC TCCTCAATCA CCAGTGCCAA CAGTGTTGAT CTTCCTGCCT GTTTCTTCTT GAAATTTCCC CAGCCAATCC CAGTATCTAG AGCATTTGTT CAGAAACTGC AGAACTGCAC AGGAATTCCA TTGTTTGAAA CTCAACCAAC TTATGCACCC CTGTATGAAC TGATCACTCA GTTTGAGCTA TCAAAGGACC CTGACCCCAT ACCTTTGAAT CACAACATGA GATTTTATGC TGCTCTTCCT GGTCAGCAGC ACTGCTATTT CCTCAACAAG GATGCTCCTC TTCCAGATGG CCGAAGTCTA CAGGGAACCC TTGTTAGCAA AATCACCTTT CAGCACCCTG GCCGAGTTCC TCTTATCCTA AATCTGATCA GACACCAAGT GGCCTATAAC ACCCTCATTG GAAGCTGTGT CAAAAGAACT ATTCTGAAAG AAGATTCTCC TGGGCTTCTC CAATTTGAAG TGTGTCCTCT CTCAGAGTCT CGTTTCAGCG TATCTTTTCA GCACCCTGTG AATGACTCCC TGGTGTGTGT GGTAATGGAT GTGCAGGACT CAACACATGT GAGCTGTAAA CTCTACAAAG GGCTGTCGGA TGCACTGATC TGCACAGATG ACTTCATTGC CAAAGTTGTT CAAAGATGTA TGTCCATCCC TGTGACGATG AGGGCTATTC GGAGGAAAGC TGAAACCATT CAAGCCGACA CCCCAGCACT GTCCCTCATT GCAGAGACAG TTGAAGACAT GGTGAAAAAG AACCTGCCCC CGGCTAGCAG CCCAGGGTAT GGCATGACCA CAGGCAACAA CCCAATGAGT GGTACCACTA CACCAACCAA CACCTTTCCG GGGGGTCCCA TTACCACCTT GTTTAATATG AGCATGAGCA TCAAAGATCG GCATGAGTCG GTGGGCCATG GGGAGGACTT CAGCAAGGTG TCTCAGAACC CAATTCTTAC CAGTTTGTTG CAAATCACAG GGAACGGGGG GTCTACCATT GGCTCGAGTC CGACCCCTCC TCATCACACG CCGCCACCTG TCTCTTCGAT GGCCGGCAAC ACCAAGAACC ACCCGATGCT CATGAACCTT CTTAAAGATA ATCCTGCCCA GGATTTCTCA ACCCTTTATG GAAGCAGCCC TTTAGAAAGG CAGAACTCCT CTTCCGGCTC ACCCCGCATG GAAATATGCT CGGGGAGCAA CAAGACCAAG AAAAAGAAGT CATCAAGATT ACCACCTGAG AAACCAAAGC ACCAGACTGA AGATGACTTT CAGAGGGAGC TATTTTCAAT GGATGTTGAC TCACAGAACC CTATCTTTGA TGTCAACATG ACAGCTGACA CGCTGGATAC GCCACACATC ACTCCAGCTC CAAGCCAGTG TAGCACTCCC CCAACAACTT ACCCACAACC AGTACCTCAC CCCCAACCCA GTATTCAAAG GATGGTCCGA CTATCCAGTT CAGACAGCAT TGGCCCAGAT GTAACTGACA TCCTTTCAGA CATTGCAGAA GAAGCTTCTA AACTTCCCAG CACTAGTGAT GATTGCCCAG CCATTGGCAC CCCTCTTCGA GATTCTTCAA GCTCTGGGCA TTCTCAGAGT ACCCTGTTTG ACTCTGATGT CTTTCAAACT AACAATAATG AAAATCCATA CACTGATCCA GCTGATCTTA TTGCAGATGC TGCTGGAAGC CCCAGTAGTG ACTCTCCTAC CAATCATTTT TTTCATGATG GAGTAGATTT CAATCCTGAT TTATTGAACA GCCAGAGCCA AAGTGGTTTT GGAGAAGAAT ATTTTGATGA AAGCAGCCAA AGTGGGGATA ATGATGATTT CAAAGGATTT GCATCTCAGG CACTAAATAC TTTGGGGGTG CCAATGCTTG GAGGTGATAA TGGGGAGACC AAGTTTAAGG GCAATAACCA AGCCGACACA GTTGATTTCA GTATTATTTC AGTAGCCGGC AAAGCTTTAG CTCCTGCAGA TCTTATGGAG CATCACAGTG GTAGTCAGGG TCCTTTACTG ACCACTGGGG ACTTAGGGAA AGAAAAGACT CAAAAGAGGG TAAAGGAAGG CAATGGCACC AGTAATAGTA CTCTCTCGGG GCCCGGATTA GACAGCAAAC CAGGGAAGCG CAGTCGGACC CCTTCTAATG ATGGGAAAAG CAAAGATAAG CCTCCAAAGC GGAAGAAGGC AGACACTGAG GGAAAGTCTC CATCTCATAG TTCTTCTAAC AGACCTTTTA CCCCACCTAC CAGTACAGGT GGATCTAAAT CGCCAGGCAG TGCAGGAAGA TCTCAGACTC CCCCAGGTGT TGCCACACCA CCCATTCCCA AAATCACTAT TCAGATTCCT AAGGGAACAG TGATGGTGGG CAAGCCTTCC TCTCACAGTC AGTATACCAG CAGTGGTTCT GTGTCTTCCT CAGGCAGCAA AAGCCACCAT AGCCATTCTT CCTCCTCTTC CTCATCTGCT TCCACCTCAG GGAAGATGAA AAGCAGTAAA TCAGAAGGTT CATCAAGTTC CAAGTTAAGT AGCAGTATGT ATTCTAGCCA GGGGTCTTCT GGATCTAGCC AGTCCAAAAA TTCATCCCAG TCTGGGGGGA AGCCAGGCTC CTCTCCCATA ACCAAGCATG GACTGAGCAG TGGCTCTAGC AGCACCAAGA TGAAACCTCA AGGAAAGCCA TCATCACTTA TGAATCCTTC TTTAAGTAAA CCAAACATAT CCCCTTCTCA TTCAAGGCCA CCTGGAGGCT CTGACAAGCT TGCCTCTCCA ATGAAGCCTG TTCCTGGAAC TCCTCCATCC TCTAAAGCCA AGTCCCCTAT CAGTTCAGGT TCTGGTGGTT CTCATATGTC TGGAACTAGT TCAAGCTCTG GCATGAAGTC ATCTTCAGGG TTAGGATCCT CAGGCTCGTT GTCCCAGAAA ACTCCCCCAT CATCTAATTC CTGTACGGCA TCTTCCTCCT CCTTTTCCTC AAGTGGCTCT TCCATGTCAT CCTCTCAGAA CCAGCATGGG AGTTCTAAAG GAAAATCTCC CAGCAGAAAC AAGAAGCCGT CCTTGACAGC TGTCATAGAT AAACTGAAGC ATGGGGTTGT CACCAGTGGC CCTGGGGGTG AAGACCCACT GGACGGCCAG ATGGGGGTGA GCACAAATTC TTCCAGCCAT CCTATGTCCT CCAAACATAA CATGTCAGGA GGAGAGTTTC AGGGCAAGCG TGAGAAAAGT GATAAAGACA AATCAAAGGT TTCCACCTCC GGGAGTTCAG TGGATTCTTC TAAGAAGACC TCAGAGTCAA AAAATGTGGG GAGCACAGGT GTGGCAAAAA TTATCATCAG TAAGCATGAT GGAGGCTCCC CTAGCATTAA AGCCAAAGTG ACTTTGCAGA AACCTGGGGA AAGTAGTGGA GAAGGGCTTA GGCCTCAAAT GGCTTCTTCT AAAAACTATG GCTCTCCACT CATCAGTGGT TCCACTCCAA AGCATGAGCG TGGCTCTCCC AGCCATAGTA AGTCACCAGC ATATACCCCC CAGAATCTGG ACAGTGAAAG TGAGTCAGGC TCCTCCATAG CAGAGAAATC TTATCAGAAT AGTCCCAGCT CAGACGATGG TATCCGACCA CTTCCAGAAT ACAGCACAGA GAAACATAAG AAGCACAAAA AGGAAAAGAA GAAAGTAAAA GACAAAGATA GGGACCGAGA CCGGGACAAA GACCGAGACA AGAAAAAATC TCATAGCATC AAGCCAGAGA GTTGGTCCAA ATCACCCATC TCTTCAGACC AGTCCTTGTC TATGACAAGT AACACAATCT TATCTGCAGA CAGACCCTCA AGGCTCAGCC CAGACTTTAT GATTGGGGAG GAAGATGATG ATCTTATGGA TGTGGCCCTG ATTGGGAATG GAGGAGGGGG AAGCGGCAAG CCCATCCCCA ACCCCCTGCT GGGCCTGGAC AGCACCGCTA GCAAAGACAA TACTGTGCCT CTGAAGCTGA TCGCTCTCCT GGCTAATGGC GAGTTCCATA GTGGCGAACA GCTGGGAGAA ACCCTGGGCA TGTCCAGGGC CGCTATCAAC AAGCACATTC AGACTCTGCG CGACTGGGGC GTGGACGTGT TCACCGTGCC CGGAAAGGGC TACTCTCTGC CCGAGCCTAT CCCGCTGCTG AACGCTAAAC AGATTCTGGG ACAGCTGGAC GGCGGGAGCG TGGCAGTCCT GCCTGTGGTC GACTCCACCA ATCAGTACCT GCTGGATCGA ATCGGCGAGC TGAAGAGTGG GGATGCTTGC ATTGCAGAAT ATCAGCAGGC AGGGAGAGGA AGCAGAGGGA GGAAATGGTT CTCTCCTTTT GGAGCTAACC TGTACCTGAG TATGTTTTGG CGCCTGAAGC GGGGACCAGC AGCAATCGGC CTGGGCCCGG TCATCGGAAT TGTCATGGCA GAAGCGCTGC GAAAGCTGGG AGCAGACAAG GTGCGAGTCA AATGGCCCAA TGACCTGTAT CTGCAGGATA GAAAGCTGGC AGGCATCCTG GTGGAGCTGG CCGGAATAAC AGGCGATGCT GCACAGATCG TCATTGGCGC CGGGATTAAC GTGGCTATGA GGCGCGTGGA GGAAAGCGTG GTCAATCAGG GCTGGATCAC ACTGCAGGAA GCAGGGATTA ACCTGGACAG GAATACTCTG GCCGCTACGC TGATCCGAGA GCTGCGGGCA GCCCTGGAAC TGTTCGAGCA GGAAGGCCTG GCTCCATATC TGCCACGGTG GGAGAAGCTG GATAACTTCA TCAATAGACC CGTGAAGCTG ATCATTGGGG ACAAAGAGAT TTTCGGGATT AGCCGGGGGA TTGATAAACA GGGAGCCCTG CTGCTGGAAC AGGACGGAGT TATCAAACCC TGGATGGGCG GAGAAATCAG TCTGCGGTCT GCCGAAAAGT AGTCTAGCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG CGGACTAGAG CGATCCCTAG GCTAGGCTTT TGCAAAGCTA GCAGCTGAAT CTAAGTGGAT CTGCGATCGC TCCGGTGCCC GTCAGTGGGC AGAGCGCACA TCGCCCACAG TCCCCGAGAA GTTGGGGGGA GGGGTCGGCA ATTGAACGGG TGCCTAGAGA AGGTGGCGCG GGGTAAACTG GGAAAGTGAT GTCGTGTACT GGCTCCGCCT TTTTCCCGAG GGTGGGGGAG AACCGTATAT AAGTGCAGTA GTCGCCGTGA ACGTTCTTTT TCGCAACGGG TTTGCCGCCA GAACACAGCT GAAGCATCGA GGGGCTCGCA TCTCTCCTTC ACGCGCCCGC CGCCCTACCT GAGGCCGCCA TCCACGCCGG TTGAGTCGCG TTCTGCCGCC TCCCGCCTGT GGTGCCTCCT GAACTGCGTC CGCCGTCTAG GTAAGTTTAA AGCTCAGGTC GAGACCGGGC CTTTGTCCGG CGCTCCCTTG GAGCCTACCT AGACTCAGCC GGCTCTCCAC GCTTTGCCTG ACCCTGCTTG CTCAACTCTA CGTCTTTGTT TCGTTTTCTG TTCTGCGCCG TTACAGATCC AAGCTGTGAC CGGCGCCTAC TCTAGAGCCG CCATGTCTAG ACTGGACAAG AGCAAAGTCA TAAACTCTGC TCTGGAATTA CTCAATGGAG TCGGTATCGA AGGCCTGACG ACAAGGAAAC TCGCTCAAAA GCTGGGAGTT GAGCAGCCTA CCCTGTACTG GCACGTGAAG AACAAGCGGG CCCTGCTCGA TGCCCTGCCA ATCGAGATGC TGGACAGGCA TCATACCCAC TCCTGCCCCC TGGAAGGCGA GTCATGGCAA GACTTTCTGC GGAACAACGC CAAGTCATAC CGCTGTGCTC TCCTCTCACA TCGCGACGGG GCTAAAGTGC ATCTCGGCAC CCGCCCAACA GAGAAACAGT ACGAAACCCT GGAAAATCAG CTCGCGTTCC TGTGTCAGCA AGGCTTCTCC CTGGAGAACG CACTGTACGC TCTGTCCGCC GTGGGCCACT TTACACTGGG CTGCGTATTG GAGGAACAGG AGCATCAAGT AGCAAAAGAG GAAAGAGAGA CACCTACCAC CGATTCTATG CCCCCACTTC TGAAACAAGC AATTGAGCTG TTCGACCGGC AGGGAGCCGA ACCTGCCTTC CTTTTCGGCC TGGAACTAAT CATATGTGGC CTGGAGAAAC AGCTAAAGTG CGAAAGCGGC GGGCCGACCG ACGCCCTTGA CGATTTTGAC TTAGACATGC TCCCAGCCGA TGCCCTTGAC GACTTTGACC TTGATATGCT GTCTGCTGAC GCTCTTGACG ATTTTGACCT TGACATGCTC CCCGGGGAGG GCAGAGGAAG TCTTCTAACA TGCGGTGACG TGGAGGAGAA TCCCGGCCCT CTTAAGATGA CCGAGTACAA GCCCACGGTG CGCCTCGCCA CCCGCGACGA CGTCCCCAGG GCCGTACGCA CCCTCGCCGC CGCGTTCGCC GACTACCCCG CCACGCGCCA CACCGTCGAT CCGGACCGCC ACATCGAGCG GGTCACCGAG CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG CTCGACATCG GCAAGGTGTG GGTCGCGGAC GACGGCGCCG CGGTGGCGGT CTGGACCACG CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG GCCCGCGCAT GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC TCCTGGCGCC GCACCGGCCC AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGCGTCTCGC CCGACCACCA GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC CCGCAACCTC CCCTTCTACG AGCGGCTCGG CTTCACCGTC ACCGCCGACG TCGAGGTGCC CGAAGGACCG CGCACCTGGT GCATGACCCG CAAGCCCGGT GCCTGAGCGG CCGCGACTCT AGTCATAATC AGCCATACCA CATTTGTAGA GGTTTTACTT GCTTTAAAAA ACCTCCCACA CCTCCCCCTG AACCTGAAAC ATAAAATGAA TGCAATTGTT GTTGTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTAGGT ACAAGTAGGC GCGTCGAGAT AACTTCGTAT AATGTATGCT ATACGAAGTT ATGTTTTGTT ACTTTATAGA AGAAATTTTG AGTTTTTGTT TTTTTTTAAT AAATAAATAA ACATAAATAA ATTGTTTGTT GAATTTATTA TTAGTATGTA AGTGTAAATA TAATAAAACT TAATATCTAT TCAAATTAAT AAATAAACGT CAATTTTACG CATGATTATC TTTAACGTAC GTCACAATAT GATTATCTTT CTAGGGTTAA AGAGCTCCAA TTCGCCCTAT AGTGAGTCGT ATTACGCGCG CTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGCTT ACAATTTAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCGCGCA ATTAACCCTC ACTAAAGGGA ACAAAAGCTG GGTACCTTAA CCCTAGAAAG ATAGTCTGCG TAAAATTGAC GCATGCATTC TTGAAATATT GCTCTCTCTT TCTAAATAGC GCGAATCCGT CGCTGTGCAT TTAGGACATC TCAGTCGCCG CTTGGAGCTC CCGTGAGGCG TGCTTGTCAA TGCGGTAAGT GTCACTGATT TTGAACTATA ACGACCGCGT GAGTCAAAAT GACGCATGAT TATCTTTTAC GTGACTTTTA AGATTTAACT CATACGATAA TTATATTGTT ATTTCATGTT CTACTTACGT GATAACTTAT TATATATATA TTTTCTTGTT ATAGATATCA TAACTTCGTA TAATGTATGC TATACGAAGT TATGCGGCC
