pSGKP-Str
$70.00
In stock
SKU
PL35742
100μg liquid plasmid(Instantly):$91
| name | pSGKP-Str | another name | apSGKP-Str |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Empty vector | Prokaryotic resistance | Str |
| Selective marker | Fluorescent labeling | ||
| Promoter | J23119 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGGGCCTAAA GAACTAAAAA ATCTATCTGT TTCTTTTCAT TCTCTGTATT TTTTATAGTT TCTGTTGCAT GGGCATAAAG TTGCAAGCTT GATATCTTGA CAGCTAGCTC AGTCCTAGGT ATAATACTAG TCGAGACCAT TGGTCTCAGT TTTAGAGCTA GAAATAGCAA GTTAAAATAA GGCTAGTCCG TTATCAACTT GAAAAAGTGG CACCGAGTCG GTGCTTTTTT TGATATCGAA TTCCTGCAGC CCGGGGGATC CACTAGTTCT AGAGCGGCCG CCACCGCGGT GGAGCTCATC CCAATGGCGC GCCGAGCTTG GCTCGAGCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTCAACCCC AAGTCAGAGG GTCCAATCGC AGATAGAAGG CAAGGCGTTC GCGGTCGGGG GCTTCGATCC CCAATACATT GAATAGGACA GCGAAGGCGC GCTCTGCTTC ATCTGGCGCT GCCCAGTTCT CTTCGGCGTT AGCAATCATG AGTGCCAAAT CGGCATAGCG ATCTGCTGTT CCGAGCCGCC CAAGGTCGAT CAGACCCGTG CATTGAAGAG TTTTAGGGTC CACCATGAAG TTCGGCATGC AGGGATCACC ATGGCAAACA ACCATATCGG TGCGCTCTTG GTCGAGCCGC ACCGGTAGCT CTCGTTCGAC ACGAGCCAAA AGATCGAGCT GCGGCGTACT CTTGTCCTCG TCCGGTAAGA AGTCGGGATT GACGGCATTG CGGGACACCA CATCAACGGC GCGTCCGAAC ATTCGCGACA GCCTGCGCTC AAACGGACAT TGATCAACCA ATAGGCTGTG AACAGCGCCA AGTTGCTGCC CCATTGACGG CCACGCTTTG AGCAAATCCG CTCCAGACAG ATCAGCCGCC GGTACTCCCG GAATTGCCGT TATCACCAAG CATGCACCCT CCTGTTCCTC CTGCCAGTTG ATCACCTCGG GGCAAGCCAC ACCTCGACCT TTGAGCCAAA TGAGGCGGTC ACGCTCTCCA GCGAGCTCAC CGCGGCGGGA AGCAGGTGCG ATTTTCGCGA AGGCATGCCC GTCACCACGT CGAAAAACAA AATCACCAGA TTCTCCGCCT CTGACAGGCA ACCAGTCAGA ATGCGATTCA CCAAAAAAAA TATTAGTTCG ATTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAACA CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA ATTGACGCGT ATTGGGATGG TACCGGGCCC CCCCTCGAGG TCGACGGTAT CGATACGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT AATCGGCATT TTCTTTTGCG TTTTTATTTG TTAACTGTTA ATTGTCCTTG TTCAAGGATG CTGTCTTTGA CAACAGATGT TTTCTTGCCT TTGATGTTCA GCAGGAAGCT AGGCGCAAAC GTTGATTGTT TGTCTGCGTA GAATCCTCTG TTTGTCATAT AGCTTGTAAT CACGACATTG TTTCCTTTCG CTTGAGGTAC AGCGAAGTGT GAGTAAGTAA AGGTTACATC GTTAGGATCA AGATCCATTT TTAACACAAG GCCAGTTTTG TTCAGCGGCT TGTATGGGCC AGTTAAAGAA TTAGAAACAT AACCAAGCAT GTAAATATCG TTAGACGTAA TGCCGTCAAT CGTCATTTTT GATCCGCGGG AGTCAGTGAA CAGATACCAT TTGCCGTTCA TTTTAAAGAC GTTCGCGCGT TCAATTTCAT CTGTTACTGT GTTAGATGCA ATCAGCGGTT TCATCACTTT TTTCAGTGTG TAATCATCGT TTAGCTCAAT CATACCGAGA GCGCCGTTTG CTAACTCAGC CGTGCGTTTT TTATCGCTTT GCAGAAGTTT TTGACTTTCT TGACGGAAGA ATGATGTGCT TTTGCCATAG TATGCTTTGT TAAATAAAGA TTCTTCGCCT TGGTAGCCAT CTTCAGTTCC AGTGTTTGCT TCAAATACTA AGTATTTGTG GCCTTTATCT TCTACGTAGT GAGGATCTCT CAGCGTATGG TTGTCGCCTG AGCTGTAGTT GCCTTCATCG ATGAACTGCT GTACATTTTG ATACGTTTTT CCGTCACCGT CAAAGATTGA TTTATAATCC TCTACACCGT TGATGTTCAA AGAGCTGTCT GATGCTGATA CGTTAACTTG TGCAGTTGTC AGTGTTTGTT TGCCGTAATG TTTACCGGAG AAATCAGTGT AGAATAAACG GATTTTTCCG TCAGATGTAA ATGTGGCTGA ACCTGACCAT TCTTGTGTTT GGTCTTTTAG GATAGAATCA TTTGCATCGA ATTTGTCGCT GTCTTTAAAG ACGCGGCCAG CGTTTTTCCA GCTGTCAATA GAAGTTTCGC CGACTTTTTG ATAGAACATG TAAATCGATG TGTCATCCGC ATTTTTAGGA TCTCCGGCTA ATGCAAAGAC GATGTGGTAG CCGTGATAGT TTGCGACAGT GCCGTCAGCG TTTTGTAATG GCCAGCTGTC CCAAACGTCC AGGCCTTTTG CAGAAGAGAT ATTTTTAATT GTGGACGAAT CGAACTCAGG AACTTGATAT TTTTCATTTT TTTGCTGTTC AGGGATTTGC AGCATATCAT GGCGTGTAAT ATGGGAAATG CCGTATGTTT CCTTATATGG CTTTTGGTTC GTTTCTTTCG CAAACGCTTG AGTTGCGCCT CCTGCCAGCA GTGCGGTAGT AAAGGTTAAT ACTGTTGCTT GTTTTGCAAA CTTTTTGATG TTCATCGTTC ATGTCTCCTT TTTTATGTAC TGTGTTAGCG GTCTGCTTCT TCCAGCCCTC CTGTTTGAAG ATGGCAAGTT AGTTACGCAC AATAAAAAAA GACCTAAAAT ATGTAAGGGG TGACGCCAAA GTATACACTT TGCCCTTTAC ACATTTTAGG TCTTGCCTGC TTTATCAGTA ACAAACCCGC GCGATTTACT TTTCGACCTC ATTCTATTAG ACTCTCGTTT GGATTGCAAC TGGTCTATTT TCCTCTTTTG TTTGATAGAA AATCATAAAA GGATTTGCAG ACTA
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