pRV-MMLV-Ubqln2(mouse)-TagBFP
$70.00
In stock
SKU
PL35510
NM_018798.2；Chap1； Dsk2； HRIHFB2157； Plic-2； Plic2
100μg liquid plasmid(Instantly):$91
| name | pRV-MMLV-Ubqln2(mouse)-TagBFP | another name | apRV-MMLV-Ubqln2(mouse)-TagBFP |
| Insert Size (bp) | 1914bp | Plasmid host | Mammary cell,retrovirus |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Blue | |
| Promoter | Replicon | pUC | |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGCCCTTTGT ACACCCTAAG CCTCCGCCTC CTCTTCCTCC ATCCGCCCCG TCTCTCCCCC TTGAACCTCC TCGTTCGACC CCGCCTCGAT CCTCCCTTTA TCCAGCCCTC ACTCCTTCTC TAGGCGCCGG AATTAGATCT CTCGAGGTTA ACGAATTCGC CACCATGGCT GAGAACGGCG AGAGCAGCGG CCCCCCGCGC CCCTCCCGCG GCCCTGCGGC GGCCCCAGGC GCGGCCAGCC CGCCGGCCGA GCCCAAAATC ATCAAAGTCA CTGTGAAGAC CCCCAAAGAG AAGGAGGAGT TCGCGGTGCC CGAGAACAGC ACCGTGCAGC AGTTCAAGGA AGCGATTTCC AAACGCTTCA AATCGCAGAC CGATCAGCTC GTGCTGATTT TCGCCGGAAA GATCCTGAAA GATCAAGATA CCTTGATGCA GCATGGCATC CATGATGGAC TGACTGTTCA CCTGGTTATC AAAAGCCAGA ACCGTCCGCA GGGCCAGGCC ACCACGCAGC CCAGCACGAC TGCGGGAACG AGCACGACGA CCACGACCAC CACCACGGCG GCGGCGCCGG CGGCGACGAC ATCATCGGCT CCCAGGAGTA GCTCCACACC TACCACCACG AATAGCAGCT CCTTTGGGCT GGGAAGCCTT TCCAGCCTTA GTAACCTGGG CTTGAACTCG CCCAACTTCA CCGAGCTTCA GAACCAGATG CAGCAGCAGC TCCTGGCCAG CCCTGAGATG ATGATCCAGA TCATGGAAAA TCCCTTTGTT CAGAGCATGC TTTCGAATCC TGATCTGATG AGGCAGCTCA TCATGGCCAA TCCACAGATG CAACAATTGA TCCAGAGAAA CCCAGAAATC AGCCACCTAC TGAACAACCC AGATATAATG AGGCAGACGC TGGAAATCGC CAGGAATCCT GCCATGATGC AAGAGATGAT GCGAAATCAA GACCTGGCTC TCAGTAATCT CGAAAGCATC CCAGGTGGCT ACAATGCTCT GCGGCGCATG TACACTGACA TTCAAGAACC TATGCTGAAT GCCGCACAGG AGCAGTTTGG GGGCAACCCG TTTGCCACGG TGGGGAGCAG TTCCACCTCA GGGGAAGGTA CTCAGCCCTC CCGCACGGAG AATCGTGATC CGCTGCCCAA TCCTTGGGCA CCACCGCCAA CAACCCAGAC GGCTGCGACC ACCACCACCA CCACGACCAC AAGCAGTGGC AGTGGCTCTG GCAGCAGCTC CAGCAGCACC ACCGCCGGGA ACACCATGGC TGCAGCTAAT TACGTGGCTA GCATCTTCAG CACCCCAGGA ATGCAGAGCC TGCTGCAGCA GATAACTGAG AATCCCCAGC TGATTCAGAA TATGCTGTCT GCGCCCTACA TGCGGAGCAT GATGCAGTCG CTGAGCCAGA ATCCAGATAT GGCGGCCCAG ATGATGCTGA GTAGCCCGCT GTTCACTTCG AATCCTCAGC TGCAGGAGCA GATGCGTCCA CAGCTCCCGA ATTTCCTGCA GCAGATGCAG AATCCAGAAA CCATCGCGGC CATGTCAAAC CCGAGAGCGA TGCAAGCGCT AATGCAGATC CAGCAGGGGC TACAGACTCT AGCCACTGAA GCACCTGGCC TCATTCCCAG CTTCGCTCCA GGTGTGGGGA TGGGAGTGCT GGGAACCGCC ATAACCCCTG TGGGCCCAGT CACTCCCATA GGGCCCATCG GCCCTATAGT TCCTTTCACC CCCATAGGCC CCATCGGGCC TATAGGACCC ACTGGCCCTG CAAGCTCCCC CGGCTCCACC GGCACTGGCA TCCCGCCTGC AACCACTGTG TCCAGCTCTG CACCTACTGA AACCATAAGT CCAACCTCGG AATCTGGACC CAACCAGCAG TTCATTCAAC AAATGGTGCA GGCTCTCACT GGAGGAAGCC CTCCACAGCC GCCGAATCCT GAAGTGAGAT TTCAGCAGCA ACTGGAACAG CTCAACGCCA TGGGGTTCTT AAACCGCGAA GCAAACTTGC AGGCCCTAAT AGCAACAGGA GGCGACATCA ACGCGGCCAT TGAGAGGCTG CTGGGCTCTC AGCCATCCGA ATTCGAGGGC AGAGGAAGTC TTCTAACATG CGGTGACGTG GAGGAGAATC CCGGCCCTAT GAGCGAGCTG ATTAAGGAGA ACATGCACAT GAAGCTGTAC ATGGAGGGCA CCGTGGACAA CCATCACTTC AAGTGCACAT CCGAGGGCGA AGGCAAGCCC TACGAGGGCA CCCAGACCAT GAGAATCAAG GTGGTCGAGG GCGGCCCTCT CCCCTTCGCC TTCGACATCC TGGCTACTAG CTTCCTCTAC GGCAGCAAGA CCTTCATCAA CCACACCCAG GGCATCCCCG ACTTCTTCAA GCAGTCCTTC CCTGAGGGCT TCACATGGGA GAGAGTCACC ACATACGAAG ACGGGGGCGT GCTGACCGCT ACCCAGGACA CCAGCCTCCA GGACGGCTGC CTCATCTACA ACGTCAAGAT CAGAGGGGTG AACTTCACAT CCAACGGCCC TGTGATGCAG AAGAAAACAC TCGGCTGGGA GGCCTTCACC GAGACGCTGT ACCCCGCTGA CGGCGGCCTG GAAGGCAGAA ACGACATGGC CCTGAAGCTC GTGGGCGGGA GCCATCTGAT CGCAAACATC AAGACCACAT ATAGATCCAA GAAACCCGCT AAGAACCTCA AGATGCCTGG CGTCTACTAT GTGGACTACA GACTGGAAAG AATCAAGGAG GCCAACAACG AGACCTACGT CGAGCAGCAC GAGGTGGCAG TGGCCAGATA CTGCGACCTC CCTAGCAAAC TGGGGCACAA GCTTAATTAA GTCGACCTGC AGCCAAGCTT ATCGATAAAA TAAAAGATTT TATTTAGTCT CCAGAAAAAG GGGGGAATGA AAGACCCCAC CTGTAGGTTT GGCAAGCTAG CTTAAGTAAC GCCATTTTGC AAGGCATGGA AAATACATAA CTGAGAATAG AGAAGTTCAG ATCAAGGTTA GGAACAGAGA GACAGCAGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGTCCCC AGATGCGGTC CCGCCCTCAG CAGTTTCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC CGAGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGCGCG CCAGTCCTCC GATAGACTGC GTCGCCCGGG TACCCGTGTA TCCAATAAAC CCTCTTGCAG TTGCATCCGA CTTGTGGTCT CGCTGTTCCT TGGGAGGGTC TCCTCTGAGT GATTGACTAC CCGTCAGCGG GGGTCTTTCA TGGGTAACAG TTTCTTGAAG TTGGAGAACA ACATTCTGAG GGTAGGAGTC GAATATTAAG TAATCCTGAC TCAATTAGCC ACTGTTTTGA ATCCACATAC TCCAATACTC CTGAAATAGT TCATTATGGA CAGCGCAGAA AGAGCTGGGG AGAATTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGCGATTAGT CCAATTTGTT AAAGACAGGA TATCAGTGGT CCAGGCTCTA GTTTTGACTC AACAATATCA CCAGCTGAAG CCTATAGAGT ACGAGCCATA GATAAAATAA AAGATTTTAT TTAGTCTCCA GAAAAAGGGG GGAATGAAAG ACCCCACCTG TAGGTTTGGC AAGCTAGCTT AAGTAACGCC ATTTTGCAAG GCATGGAAAA TACATAACTG AGAATAGAGA AGTTCAGATC AAGGTTAGGA ACAGAGAGAC AGCAGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG GCTCAGGGCC AAGAACAGAT GGTCCCCAGA TGCGGTCCCG CCCTCAGCAG TTTCTAGAGA ACCATCAGAT GTTTCCAGGG TGCCCCAAGG ACCTGAAATG ACCCTGTGCC TTATTTGAAC TAACCAATCA GTTCGCTTCT CGCTTCTGTT CGCGCGCTTC TGCTCCCCGA GCTCAATAAA AGAGCCCACA ACCCCTCACT CGGCGCGCCA GTCCTCCGAT AGACTGCGTC GCCCGGGTAC CCGTATTCCC AATAAAGCCT CTTGCTGTTT GCATCCGAAT CGTGGACTCG CTGATCCTTG GGAGGGTCTC CTCAGATTGA TTGACTGCCC ACCTCGGGGG TCTTTCATTT GGAGGTTCCA CCGAGATTTG GAGACCCCTG CCCAGGGACC ACCGACCCCC CCGCCGGGAG GTAAGCTGGC CAGCGGTCGT TTCGTGTCTG TCTCTGTCTT TGTGCGTGTT TGTGCCGGCA TCTAATGTTT GCGCCTGCGT CTGTACTAGT TAGCTAACTA GCTCTGTATC TGGCGGACCC GTGGTGGAAC TGACGAGTTC TGAACACCCG GCCGCAACCC TGGGAGACGT CCCAGGGACT TTGGGGGCCG TTTTTGTGGC CCGACCTGAG GAAGGGAGTC GATGTGGAAT CCGACCCCGT CAGGATATGT GGTTCTGGTA GGAGACGAGA ACCTAAAACA GTTCCCGCCT CCGTCTGAAT TTTTGCTTTC GGTTTGGAAC CGAAGCCGCG CGTCTTGTCT GCTGCAGCGC TGCAGCATCG TTCTGTGTTG TCTCTGTCTG ACTGTGTTTC TGTATTTGTC TGAAAATTAG GGCCAGACTG TTACCACTCC CTTAAGTTTG ACCTTAGGTC ACTGGAAAGA TGTCGAGCGG ATCGCTCACA ACCAGTCGGT AGATGTCAAG AAGAGACGTT GGGTTACCTT CTGCTCTGCA GAATGGCCAA CCTTTAACGT CGGATGGCCG CGAGACGGCA CCTTTAACCG AGACCTCATC ACCCAGGTTA AGATCAAGGT CTTTTCACCT GGCCCGCATG GACACCCAGA CCAGGTCCCC TACATCGTGA CCTGGGAAGC CTTGGCTTTT GACCCCCCTC CCTGGGTCA
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