pCMV-NCOR1(human)-3×Myc-Neo
$157.00
In stock
SKU
PL35475
NM_006311.4；hN-CoR; N-CoR; N-CoR1; PPP1R109; TRAC1
100μg liquid plasmid(Instantly):$178
| name | pCMV-NCOR1(human)-3×Myc-Neo | another name | apCMV-NCOR1(human)-3×Myc-Neo |
| Insert Size (bp) | 7320bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCATGTC AAGTTCAGGT TATCCTCCCA ACCAAGGAGC ATTCAGCACA GAACAAAGTC GTTATCCTCC TCACTCTGTC CAGTATACAT TTCCCAACAC CCGCCACCAG CAGGAGTTCG CAGTCCCTGA TTATCGTTCC TCTCATCTTG AAGTGAGTCA GGCATCACAG CTTTTGCAGC AACAGCAGCA GCAACAGCTT CGAAGGCGAC CTTCCTTGCT TTCAGAATTT CACCCAGGTT CTGACAGGCC TCAAGAAAGG AGAACTAGTT ATGAACCGTT TCATCCAGGC CCATCCCCAG TGGATCATGA TTCACTGGAA TCGAAGCGAC CACGTCTGGA ACAGGTTTCT GATTCTCATT TTCAGCGTGT CAGTGCTGCG GTTTTGCCTT TAGTGCACCC GCTGCCAGAA GGGCTGAGGG CTTCTGCAGA TGCTAAGAAG GATCCAGCAT TCGGAGGCAA ACATGAAGCT CCATCCTCTC CAATTTCGGG GCAACCATGT GGAGATGATC AAAATGCTTC ACCTTCAAAA CTCTCAAAGG AAGAGTTAAT ACAGAGTATG GATCGTGTAG ATCGAGAAAT TGCAAAAGTA GAACAGCAGA TCCTTAAACT GAAAAAGAAA CAACAACAGC TTGAAGAAGA GGCAGCTAAA CCTCCTGAGC CTGAGAAGCC CGTGTCCCCT CCTCCTGTGG AGCAGAAACA CCGCAGTATT GTCCAAATTA TTTATGATGA GAATCGGAAA AAAGCAGAAG AAGCTCATAA AATTTTTGAA GGTCTTGGCC CAAAAGTTGA ACTGCCACTG TATAACCAGC CATCAGATAC CAAGGTGTAC CATGAGAACA TCAAGACAAA CCAGGTGATG AGGAAAAAAC TCATTTTATT TTTTAAAAGA AGAAATCATG CAAGAAAACA AAGGGAACAA AAAATCTGCC AGCGTTATGA TCAGCTCATG GAGGCATGGG AGAAAAAAGT GGACAGAATA GAAAATAATC CTCGGAGGAA AGCTAAAGAA AGCAAAACAA GGGAATACTA TGAAAAGCAG TTTCCAGAAA TTCGAAAACA AAGAGAACAG CAAGAAAGAT TTCAGCGAGT TGGGCAGAGG GGAGCTGGTC TTTCAGCCAC CATTGCTAGG AGTGAGCATG AGATTTCTGA AATTATTGAT GGGCTCTCTG AGCAGGAGAA TAATGAGAAA CAAATGCGGC AGCTCTCTGT GATTCCACCT ATGATGTTTG ATGCAGAACA AAGACGAGTC AAGTTCATTA ACATGAATGG GCTTATGGAG GACCCTATGA AAGTGTATAA AGATAGGCAG TTTATGAATG TTTGGACTGA CCATGAAAAG GAGATCTTTA AGGACAAGTT TATCCAGCAT CCAAAAAACT TTGGACTAAT TGCATCATAC TTGGAGAGGA AGAGTGTTCC TGATTGTGTT TTGTATTACT ATTTAACCAA GAAAAATGAG AATTATAAAG CCCTCGTCAG AAGGAATTAT GGGAAACGCA GAGGCAGAAA CCAGCAAATT GCTCGACCCT CGCAAGAAGA AAAAGTAGAA GAAAAAGAAG AGGATAAAGC AGAAAAAACA GAAAAAAAAG AAGAAGAAAA GAAAGATGAA GAGGAAAAAG ATGAAAAAGA AGACTCCAAA GAAAATACCA AGGAAAAGGA CAAGATAGAT GGTACAGCAG AAGAAACTGA GGAAAGAGAG CAAGCCACAC CCCGGGGGCG AAAGACTGCC AACAGTCAGG GCCGCCGTAA GGGCCGGATC ACCAGGTCCA TGACAAACGA AGCTGCAGCT GCCAGTGCTG CAGCCGCAGC GGCTACTGAA GAGCCCCCAC CACCTCTGCC ACCGCCACCA GAACCCATTT CTACAGAGCC TGTGGAGACC TCTCGATGGA CAGAAGAAGA AATGGAAGTT GCTAAAAAAG GTCTAGTAGA ACATGGTCGT AACTGGGCAG CAATTGCTAA AATGGTGGGA ACGAAAAGTG AAGCTCAATG TAAAAACTTC TATTTTAACT ATAAAAGGCG ACACAATCTT GACAACCTCT TACAGCAGCA TAAACAGAAA ACTTCACGAA AACCTCGTGA AGAGCGAGAT GTGTCTCAAT GTGAAAGTGT CGCTTCCACT GTTTCTGCTC AGGAGGATGA AGATATTGAA GCCTCCAATG AAGAAGAAAA TCCAGAAGAC AGCGAAGTTG AAGCTGTCAA GCCCAGCGAG GACAGTCCTG AAAATGCTAC TTCTCGAGGA AACACAGAAC CTGCGGTTGA GCTTGAGCCC ACCACGGAAA CTGCACCCAG TACATCTCCC TCCTTAGCAG TTCCAAGTAC AAAACCAGCT GAAGATGAAA GTGTGGAGAC CCAGGTGAAT GACAGCATCA GTGCTGAGAC AGCAGAGCAG ATGGATGTAG ATCAGCAGGA GCACAGTGCT GAAGAGGGTT CTGTTTGTGA TCCCCCACCC GCTACCAAAG CTGACTCTGT GGACGTTGAA GTGAGGGTGC CAGAAAACCA TGCATCTAAA GTTGAAGGTG ATAATACCAA AGAAAGAGAC TTGGATAGAG CCAGTGAGAA GGTGGAACCT AGAGATGAAG ATTTGGTGGT AGCTCAGCAA ATAAATGCCC AAAGGCCCGA GCCCCAGTCA GACAATGATT CCAGTGCCAC GTGCAGCGCT GATGAGGATG TGGATGGAGA GCCAGAGAGG CAGAGAATGT TTCCTATGGA CTCAAAGCCT TCACTGTTAA ACCCCACTGG ATCTATACTC GTCTCATCTC CGTTAAAACC AAATCCACTG GATCTGCCAC AGCTTCAGCA TCGAGCTGCT GTTATCCCAC CAATGGTATC CTGCACCCCA TGTAACATAC CAATTGGAAC CCCAGTGAGC GGCTATGCTC TCTACCAGCG ACACATTAAA GCAATGCATG AGTCAGCACT CCTGGAGGAG CAGCGGCAGA GACAAGAACA GATAGATTTG GAATGTAGAA GTTCTACAAG TCCATGTGGC ACATCCAAGA GTCCAAACAG AGAGTGGGAA GTCCTTCAGC CTGCTCCACA TCAAGTGATA ACTAATCTCC CTGAAGGCGT TCGGCTTCCG ACAACTCGAC CAACCAGGCC ACCGCCCCCT CTCATCCCGT CATCCAAAAC CACAGTGGCT TCAGAAAAAC CATCTTTTAT AATGGGAGGC TCCATCTCAC AGGGAACACC AGGCACTTAT TTGACTTCTC ATAATCAGGC TTCCTACACT CAAGAAACAC CCAAGCCGTC AGTGGGATCT ATCTCTCTTG GACTGCCACG GCAACAGGAA TCTGCCAAAT CAGCTACTTT GCCCTACATC AAGCAGGAAG AATTTTCTCC CCGAAGCCAA AACTCACAAC CTGAGGGTCT GTTGGTCAGG GCCCAACATG AAGGTGTAGT CAGAGGTACC GCAGGAGCCA TACAAGAAGG AAGTATAACT CGGGGAACTC CAACCAGCAA AATTTCAGTG GAGAGCATTC CATCCCTACG GGGCTCTATC ACTCAGGGCA CCCCGGCTCT GCCCCAGACT GGCATACCAA CAGAGGCTTT GGTGAAGGGG TCCATTTCGA GAATGCCCAT TGAAGACAGC AGTCCTGAGA AAGGCAGAGA GGAAGCTGCA TCCAAAGGCC ATGTTATTTA TGAAGGCAAA AGTGGACATA TCTTGTCATA TGATAATATT AAGAATGCCC GAGAAGGGAC TAGGAGTCCA AGAACAGCTC ATGAAATCAG TTTAAAGAGA AGCTATGAAT CAGTGGAAGG AAATATAAAG CAAGGGATGT CAATGAGGGA GTCTCCTGTA TCAGCACCGT TAGAGGGGCT GATATGCCGA GCATTACCCA GGGGGAGTCC TCATTCTGAC CTCAAAGAAA GGACTGTATT GTCTGGCTCC ATAATGCAGG GGACACCAAG AGCAACAACT GAAAGCTTTG AAGATGGCCT TAAATATCCC AAACAAATTA AAAGGGAAAG TCCTCCCATA CGAGCATTTG AAGGTGCCAT TACCAAAGGA AAACCATATG ATGGCATCAC CACCATCAAA GAAATGGGGC GTTCCATTCA TGAGATTCCA AGGCAAGATA TTTTAACTCA GGAAAGTCGG AAAACTCCAG AAGTGGTCCA GAGCACACGG CCGATAATTG AGGGTTCCAT TTCCCAGGGC ACACCAATAA AGTTTGACAA CAACTCAGGT CAATCTGCCA TCAAACACAA TGTCAAATCC TTAATCACGG GGCCTAGCAA ACTATCCCGT GGAATGCCTC CGCTGGAAAT TGTGCCAGAG AACATAAAAG TGGTAGAACG GGGAAAATAT GAGGATGTGA AAGCAGGCGA GACCGTGCGT TCCCGGCACA CGTCAGTGGT AAGCTCTGGC CCCTCCGTTC TTAGGTCCAC ACTGCATGAA GCTCCCAAAG CACAACTGAG CCCTGGGATT TATGATGACA CCAGTGCACG GAGGACCCCT GTGAGTTATC AAAACACCAT GTCCAGAGGC TCACCCATGA TGAACAGAAC TTCTGATGTT ACAATTTCTT CTAACAAGTC TACCAATCAT GAAAGGAAAT CGACACTGAC CCCTACCCAG AGGGAAAGTA TCCCAGCGAA GTCTCCAGTG CCTGGGGTGG ACCCTGTCGT GAGCCACAGT CCGTTTGATC CCCATCACAG AGGCAGCACT GCAGGCGAGG TTTATCGGAG CCACCTGCCC ACGCACTTGG ATCCAGCCAT GCCTTTTCAC AGGGCTTTGG ATCCTGCAGC GGCTGCTTAC CTGTTTCAGA GACAGCTTTC ACCAACTCCA GGTTACCCAA GTCAGTATCA GCTTTACGCA ATGGAGAACA CAAGACAGAC AATCTTAAAT GATTACATTA CCTCACAACA GATGCAAGTG AACTTGCGTC CAGATGTGGC CAGAGGACTC TCCCCAAGAG AGCAGCCACT GGGTCTCCCA TACCCAGCAA CGAGAGGAAT CATTGACCTG ACCAATATGC CTCCAACAAT TTTAGTGCCT CATCCAGGGG GAACAAGCAC TCCTCCCATG GACAGAATCA CTTATATTCC TGGTACACAG ATTACTTTCC CTCCCAGGCC GTACAACTCT GCTTCCATGT CTCCAGGACA CCCAACACAC CTTGCAGCTG CTGCAAGTGC TGAGAGGGAA CGGGAACGGG AGCGGGAGAA GGAGCGGGAG CGGGAACGGA TTGCTGCAGC TTCCTCCGAC CTCTACCTGC GGCCAGGCTC AGAACAGCCT GGCCGACCTG GCAGTCATGG ATATGTTCGC TCCCCTTCCC CTTCAGTAAG AACTCAGGAG ACCATGTTGC AACAGAGACC CAGTGTTTTC CAAGGAACCA ATGGAACCAG TGTAATCACA CCTTTGGATC CAACTGCTCA GCTACGAATC ATGCCACTGC CTGCTGGGGG CCCTTCAATA AGCCAAGGCC TGCCAGCCTC CCGTTACAAC ACTGCTGCGG ATGCCCTGGC TGCTCTTGTG GATGCTGCAG CTTCTGCACC CCAGATGGAT GTGTCCAAAA CAAAAGAGAG TAAGCATGAA GCTGCCAGGT TAGAAGAAAA TTTGAGAAGC AGGTCAGCAG CAGTTAGTGA ACAGCAGCAG CTAGAGCAGA AAACCCTGGA GGTGGAGAAG AGATCTGTTC AGTGTTTATA CACTTCTTCA GCCTTTCCAA GTGGCAAGCC CCAGCCTCAT TCTTCAGTAG TTTATTCTGA GGCTGGGAAA GATAAAGGGC CTCCTCCAAA ATCCAGATAT GAGGAAGAGC TAAGGACCAG AGGGAAGACT ACCATTACTG CAGCTAACTT CATAGACGTG ATCATCACCC GGCAAATTGC CTCGGACAAG GATGCGAGGG AACGTGGCTC TCAAAGTTCA GACTCTTCTA GTAGCTTATC TTCTCACAGG TATGAAACAC CTAGCGATGC TATTGAGGTG ATAAGTCCTG CCAGCTCACC TGCGCCACCC CAGGAGAAAC TGCAGACCTA TCAGCCAGAG GTTGTTAAGG CAAATCAAGC GGAAAATGAT CCTACCAGAC AATATGAAGG ACCATTACAT CACTATCGAC CACAGCAGGA ATCACCATCT CCCCAACAAC AGCTGCCCCC TTCTTCACAG GCAGAGGGAA TGGGGCAAGT GCCCAGGACC CATCGGCTGA TCACACTTGC TGATCACATC TGTCAAATTA TCACACAAGA TTTTGCTAGA AATCAAGTTT CCTCGCAGAC TCCCCAGCAG CCTCCTACTT CTACATTCCA GAACTCACCT TCTGCTTTGG TATCTACACC TGTGAGGACT AAAACATCAA ACCGTTACAG CCCAGAATCC CAGGCTCAGT CTGTCCATCA TCAAAGACCA GGTTCAAGGG TCTCTCCAGA AAATCTTGTG GACAAATCCA GGGGAAGTAG GCCTGGAAAA TCCCCAGAGA GGAGTCACGT CTCTTCGGAG CCCTACGAGC CCATCTCCCC ACCCCAGGTT CCGGTTGTGC ATGAGAAACA GGACAGCTTG CTGCTCTTGT CTCAGAGGGG CGCAGAGCCT GCAGAGCAGA GGAATGATGC CCGCTCACCA GGGAGTATAA GCTACTTGCC TTCATTCTTC ACCAAGCTTG AAAATACATC ACCCATGGTT AAATCAAAGA AGCAGGAGAT TTTTCGTAAG TTGAACTCCT CTGGTGGAGG TGACTCTGAT ATGGCAGCTG CTCAGCCAGG AACTGAGATC TTTAATCTGC CAGCAGTTAC TACGTCAGGC TCAGTTAGCT CTAGAGGCCA TTCTTTTGCT GATCCTGCCA GTAATCTTGG GCTGGAAGAC ATTATCAGGA AGGCTCTCAT GGGAAGCTTT GATGACAAAG TTGAGGATCA TGGAGTTGTC ATGTCCCAGC CTATGGGAGT AGTGCCTGGT ACTGCCAACA CCTCAGTTGT GACCAGTGGT GAGACACGAA GAGAGGAAGG GGACCCATCA CCTCATTCAG GAGGAGTTTG CAAACCAAAG CTGATCAGCA AGTCAAACAG CAGGAAATCT AAGTCTCCTA TACCTGGGCA AGGCTACTTA GGAACGGAAC GGCCCTCTTC AGTCTCCTCT GTACATTCAG AAGGGGATTA CCATAGGCAG ACGCCAGGGT GGGCCTGGGA AGACAGGCCC TCTTCAACAG GCTCAACTCA GTTTCCTTAT AACCCTCTGA CTATGCGGAT GCTCAGCAGT ACTCCACCAA CACCGATTGC ATGTGCTCCC TCTGCGGTGA ACCAAGCAGC TCCTCACCAA CAGAACAGGA TCTGGGAGCG AGAGCCTGCC CCACTGCTCT CAGCACAGTA CGAGACCCTG TCGGATAGTG ATGACCTCGA GGAACAAAAA CTAATATCAG AGGAAGATTT GGAACAGAAA CTAATCAGTG AAGAAGATTT AGAGCAAAAG TTAATTTCCG AGGAGGACTT ATAGTGAGGG CCCGATATCT CGACAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAG CTGACGTCCT TTCCATGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCCTGGAAA CGGGGGAGGC TAACTGAAAC ACGGAAGGAG ACAATACCGG AAGGAACCCG CGCTATGACG GCAATAAAAA GACAGAATAA AACGCACGGG TGTTGGGTCG TTTGTTCATA AACGCGGGGT TCGGTCCCAG GGCTGGCACT CTGTCGATAC CCCACCGAGA CCCCATTGGG GCCAATACGC CCGCGTTTCT TCCTTTTCCC CACCCCACCC CCCAAGTTCG GGTGAAGGCC CAGGGCTCGC AGCCAACGTC GGGGCGGCAG GCCCTGCCAT AGCAGATCTG CGCAGCTGGG GCTCTAGGGG GTATCCCCAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGCATCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGG GGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCAATGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCGACGGA TCGGGAGATC TCCCGATCCC CTATGGTCGA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATCTGCTC CCTGCTTGTG TGTTGGAGGT CGCTGAGTAG TGCGCGAGCA AAATTTAAGC TACAACAAGG CAAGGCTTGA CCGACAATTG CATGAAGAAT CTGCTTAGG
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