pMSP3535-CRE
$70.00
In stock
SKU
PL35442
100μg liquid plasmid(Instantly):$91
| name | pMSP3535-CRE | another name | apMSP3535-CRE |
| Insert Size (bp) | Plasmid host | Gram-positive bacteria | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Ery | |
| Selective marker | Fluorescent labeling | ||
| Promoter | PnisA | Replicon | pUC |
| Competence | MC1061 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGATCTGATC CGTAGCGGTT TTCAAAATTT GCAACCAGGA ATGAATTACT ATCCCTTTTA TCAAGAAGCG CAAAAGAAAA ACGAAATGAT ACACCAATCA GTGCAAAAAA AGATATAATG GGAGATAAGA CGGTTCGTGT TCGTGCTGAC TTGCACCATA TCATAAAAAT CGAAACAGCA AAGAATGGCG GAAACGTAAA AGAAGTTATG GAAATAAGAC TTAGAAGCAA ACTTAAGAGT GTGTTGATAG TGCAGTATCT TAAAATTTTG TATAATAGGA ATTGAAGTTA AATTAGATGC TAAAAATTTG TAATTAAGAA GGAGTGATTA CATGAACAAA AATATAAAAT ATTCTCAAAA CTTTTTAACG AGTGAAAAAG TACTCAACCA AATAATAAAA CAATTGAATT TAAAAGAAAC CGATACCGTT TACGAAATTG GAACAGGTAA AGGGCATTTA ACGACGAAAC TGGCTAAAAT AAGTAAACAG GTAACGTCTA TTGAATTAGA CAGTCATCTA TTCAACTTAT CGTCAGAAAA ATTAAAACTG AATACTCGTG TCACTTTAAT TCACCAAGAT ATTCTACAGT TTCAATTCCC TAACAAACAG AGGTATAAAA TTGTTGGGAG TATTCCTTAC CATTTAAGCA CACAAATTAT TAAAAAAGTG GTTTTTGAAA GCCATGCGTC TGACATCTAT CTGATTGTTG AAGAAGGATT CTACAAGCGT ACCTTGGATA TTCACCGAAC ACTAGGGTTG CTCTTGCACA CTCAAGTCTC GATTCAGCAA TTGCTTAAGC TGCCAGCGGA ATGCTTTCAT CCTAAACCAA AAGTAAACAG TGTCTTAATA AAACTTACCC GCCATACCAC AGATGTTCCA GATAAATATT GGAAGCTATA TACGTACTTT GTTTCAAAAT GGGTCAATCG AGAATATCGT CAACTGTTTA CTAAAAATCA GTTTCATCAA GCAATGAAAC ACGCCAAAGT AAACAATTTA AGTACCGTTA CTTATGAGCA AGTATTGTCT ATTTTTAATA GTTATCTATT ATTTAACGGG AGGAAATAAT TCTATGAGTC GCTTTTGTAA ATTTGGAAAG TTACACGTTA CTAAAGGGAA TGTAGATAAA TTATTAGGTA TACTACTGAC AGCTTCCAAG GAGCTAAAGA GGTCCCTAGC GCTTAGAATC GCTTTAGGAA ACACGATCCA GTCCAATAAT CGTCGATAAA AACTTTTGAA AAAGGTTGGT GAAATTACCT ACTTTTGGAA TAATCACAAA TCACAAGTGA TTAATCACAA ATCACAAGTG ATTAATCACT TGTTTATTAA GATATTAAAA GCTATAATTT AAATAAAGCG TGAATTTTAT TACACAAAAA GAGGGGGGAG AAACTTGGAA CTAGCATTTA GAGAAAGCTT AAAAAAGATG AGAGGTACCA AATCAAAAGA AAAATTCTCC CAAGAATTAG AAATGAGTAG ATCAAATTAT TCACGAATAG AATCAGGAAA ATCAGATCCA ACCATAAAAA CACTAGAACA AATTGCAAAG TTAACTAACT CAACGCTAGT AGTGGATTTA ATCCCAAATG AGCCAACAGA ACCAGAACCA GAAACAGAAT CAGAACAAGT AACATTGGAT TTAGAAATGG AAGAAGAAAA AAGCAATGAC TTCGTGTGAA TAATGCACGA AATCGTTGCT TATTTTTTTT TAAAAGCGGT ATACTAGATA TAACGAAACA ACGAACTGAA TAGAAACGAA AAAAGAGCCA TGACACATTT ATAAAATGTT TGACGACATT TTATAAATGC ATAGCCCGAT AAGATTGCCA AACCAACGCT TATCAGTTAG TCAGATGAAC TCTTCCCTCG TAAGAAGTTA TTTAATTAAC TTTGTTTGAA GACGGTATAT AACCGTACTA TCATTATATA GGGAAATCAG AGAGTTTTCA AGTATCTAAG CTACTGAATT TAAGAATTGT TAAGCAATCA ATCGGAAATC GTTTGATTGC TTTTTTTGTA TTCATTTATA GAAGGTGGAG TTTGTATGAA TCATGATGAA TGTAAAACTT ATATAAAAAA TAGTTTATTG GAGATAAGAA AATTAGCAAA TATCTATACA CTAGAAACGT TTAAGAAAGA GTTAGAAAAG AGAAATATCT ACTTAGAAAC AAAATCAGAT AAGTATTTTT CTTCGGAGGG GGAAGATTAT ATATATAAGT TAATAGAAAA TAACAAAATA ATTTATTCGA TTAGTGGAAA AAAATTGACT TATAAAGGAA AAAAATCTTT TTCAAAACAT GCAATATTGA AACAGTTGAA TGAAAAAGCA AACCAAGTTA ATTAAACAAC CTATTTTATA GGATTTATAG GAAAGGAGAA CAGCTGAATG AATATCCCTT TTGTTGTAGA AACTGTGCTT CATGACGGCT TGTTAAAGTA CAAATTTAAA AATAGTAAAA TTCGCTCAAT CACTACCAAG CCAGGTAAAA GCAAAGGGGC TATTTTTGCG TATCGCTCAA AATCAAGCAT GATTGGCGGT CGTGGTGTTG TTCTGACTTC CGAGGAAGCG ATTCAAGAAA ATCAAGATAC ATTTACACAT TGGACACCCA ACGTTTATCG TTATGGAACG TATGCAGACG AAAACCGTTC ATACACGAAA GGACATTCTG AAAACAATTT AAGACAAATC AATACCTTCT TTATTGATTT TGATATTCAC ACGGCAAAAG AAACTATTTC AGCAAGCGAT ATTTTAACAA CCGCTATTGA TTTAGGTTTT ATGCCTACTA TGATTATCAA ATCTGATAAA GGTTATCAAG CATATTTTGT TTTAGAAACG CCAGTCTATG TGACTTCAAA ATCAGAATTT AAATCTGTCA AAGCAGCCAA AATAATTTCG CAAAATATCC GAGAATATTT TGGAAAGTCT TTGCCAGTTG ATCTAACGTG TAATCATTTT GGTATTGCTC GCATACCAAG AACGGACAAT GTAGAATTTT TTGATCCTAA TTACCGTTAT TCTTTCAAAG AATGGCAAGA TTGGTCTTTC AAACAAACAG ATAATAAGGG CTTTACTCGT TCAAGTCTAA CGGTTTTAAG CGGTACAGAA GGCAAAAAAC AAGTAGATGA ACCCTGGTTT AATCTCTTAT TGCACGAAAC GAAATTTTCA GGAGAAAAGG GTTTAATAGG GCGTAATAAC GTCATGTTTA CCCTCTCTTT AGCCTACTTT AGTTCAGGCT ATTCAATCGA AACGTGCGAA TATAATATGT TTGAGTTTAA TAATCGATTA GATCAACCCT TAGAAGAAAA AGAAGTAATC AAAATTGTTA GAAGTGCCTA TTCAGAAAAC TATCAAGGGG CTAATAGGGA ATACATTACC ATTCTTTGCA AAGCTTGGGT ATCAAGTGAT TTAACCAGTA AAGATTTATT TGTCCGTCAA GGGTGGTTTA AATTCAAGAA AAAAAGAAGC GAACGTCAAC GTGTTCATTT GTCAGAATGG AAAGAAGATT TAATGGCTTA TATTAGCGAA AAAAGCGATG TATACAAGCC TTATTTAGTG ACGACCAAAA AAGAGATTAG AGAAGTGCTA GGCATTCCTG AACGGACATT AGATAAATTG CTGAAGGTAC TGAAGGCGAA TCAGGAAATT TTCTTTAAGA TTAAACCAGG AAGAAATGGT GGCATTCAAC TTGCTAGTGT TAAATCATTG TTGCTATCGA TCATTAAAGT AAAAAAAGAA GAAAAAGAAA GCTATATAAA GGCGCTGACA AATTCTTTTG ACTTAGAGCA TACATTCATT CAAGAGACTT TAAACAAGCT AGCAGAACGC CCTAAAACGG ACACACAACT CGATTTGTTT AGCTATGATA CAGGCTGAAA ATAAAACCCG CACTATGCCA TTACATTTAT ATCTATGATA CGTGTTTGTT TTTTCTTTGC TGTTTAGCGA ATGATTAGCA GAAATATACA GAGTAAGATT TTAATTAATT ATTAGGGGGA GAAGGAGAGA GTAGCCCGAA AACTTTTAGT TGGCTTGGAC TGAACGAAGT GAGGGAAAGG CTACTAAAAC GTCGAGGGGC AGTGAGAGCG AAGCGAACAC TTGATTTTTT AATTTTCTAT CTTTTATAGG TCATTAGAGT ATACTTATTT GTCCTATAAA CTATTTAGCA GCATAATAGA TTTATTGAAT AGGTCATTTA AGTTGAGCAT ATTAGAGGAG GAAAATCTTG GAGAAATATT TGAAGAACCC GATTACATGG ATTGGATTAG TTCTTGTGGT TACGTGGTTT TTAACTAAAA GTAGTGAATT TTTGATTTTT GGTGTGTGTG TCTTGTTGTT AGTATTTGCT AGTCAAAGTG ATTAAATAGA ATTCTCATGT TTGACAGCTT ATCATCGGAG CTCCGATGAT AAGCTGTCCA AACATGAGAA TTCCCCGGCT TTAGGTATAG TGTGTATCTC AATCCTTGGT ATATTGAAAA GAAAGACTAA AAATTGATAG ATTATATTTC TTCAGAATGA ATGGTATAAT GAAGTAATGA GTACTAAACA ATCGGAGGTA AAGTGGTGTA TAAAATTTTA ATAGTTGATG ATGATCAGGA AATTTTAAAA TTAATGAAGA CAGCATTAGA AATGAGAAAC TATGAAGTTG CGACGCATCA AAACATTTCA CTTCCCTTGG ATATTACTGA TTTTCAGGGA TTTGATTTGA TTTTGTTAGA TATCATGATG TCAAATATTG AAGGGACAGA AATTTGTAAA AGGATTCGCA GAGAAATATC AACTCCAATT ATCTTTGTTA GTGCGAAAGA TACAGAAGAG GATATTATAA ACGGCTTAGG TATTGGTGGG GATGACTATA TTACTAAGCC TTTTAGCCTT AAACAGTTGG TTGCAAAAGT GGAAGCAAAT ATAAAGCGAG AGGAACGCAA TAAACATGCA GTTCATGTTT TTTCAGAGAT TCGTAGAGAT TTAGGACCAA TTACATTTTA TTTAGAAGAA AGGCGAGTCT GTGTCAATGG TCAAACAATT CCACTGACTT GTCGTGAATA CGATATTCTT GAATTACTAT CACAACGAAC TTCTAAAGTT TATACGAGAG AGGATATTTA TGATGACGTA TATGATGAAT ATTCTAATGC ACTTTTTCGG TCAATCTCGG AGTATATTTA TCAGATTAGG AGTAAGTTTG CACCATACGA TATTAATCCG ATAAAAACGG TTCGGGGACT TGGGTATCAG TGGCATGGGT AAAAAATATT CAATGCGTCG ACGGATATGG CAAGCTGTCA TTGAAATTAT CATAGGTACT TGTCTACTTA TCCTGTTGTT ACTGGGCTTG ACTTTCTTTC TACGACAAAT TGGACAAATC AGTGGTTCAG AAACTATTCG TTTATCTTTA GATTCAGATA ATTTAACTAT TTCTGATATC GAACGTGATA TGAAACACTA CCCATATGAT TATATTATTT TTGACAATGA TACAAGTAAA ATTTTGGGAG GACATTATGT CAAGTCGGAT GTACCTAGTT TTGTAGCTTC AAAACAGTCT TCACATAATA TTACAGAAGG AGAAATTACT TATACTTATT CAAGCAATAA GCATTTTTCA GTTGTTTTAA GACAAAACAG TATGCCTGAA TTTACAAATC ATACGCTTCG TTCAATTTCT TATAATCAAT TTACTTACCT TTTCTTTTTT CTTGGTGAAA TAATACTCAT TATTTTTTCT GTCTATCATC TCATTAGAGA ATTTTCTAAG AATTTTCAAG CCGTTCAAAA GATTGCATTG AAGATGGGGG AAATAACTAC TTTTCCTGAA CAAGAGGAAT CAAAAATTAT TGAATTTGAT CAGGTTCTGA ATAACTTATA TTCGAAAAGT AAGGAGTTAG CTTTCCTTAT TGAAGCGGAG CGTCATGAAA AACATGATTT ATCCTTCCAG GTTGCTGCAC TTTCACATGA TGTTAAGACA CCTTTAACAG TATTAAAAGG AAATATTGAA CTGCTAGAGA TGACTGAAGT AAATGAACAA CAAGCTGATT TTATTGAGTC AATGAAAAAT AGTTTGACTG TTTTTGACAA GTATTTTAAC ACAATGATTA GTTATACAAA ACTTTTGAAT GATGAAAATG ATTACAAAGC GACAATCTCC CTGGAGGATT TTTTGATAGA TTTATCAGTT GAGTTGGAAG AGTTGTCAAC AACTTATCAA GTGGATTATC AGCTAGTTAA AAAAACAGAT TTAACCACTT TTTACGGAAA TACATTAGCT TTAAGTCGAG CACTTATCAA TATCTTTGTT AATGCCTGTC AGTATGCTAA AGAGGGTGAA AAAATAGTCA GTTTGAGTAT TTATGATGAT GAAAAATATC TCTATTTTGA AATCTGGAAT AATGGTCATC CTTTTTCTGA ACAAGCAAAA AAAAATGCTG GAAAACTATT TTTCACAGAA GATACTGGAC GTAGTGGGAA ACACTATGGG ATTGGACTAT CTTTTGCTCA AGGTGTAGCT TTAAAACATC AAGGAAACTT AATTCTCAGT AATCCTCAAA AAGGTGGGGC AGAAGTTATC CTAAAAATAA AAAAGTAATT TAGTAATCTC TAAGGATTAC TTTTTTTGTT TCGGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CATTGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGGTTAAC CTGGCTTATC GAAATTAATA CGACTCACTA TAGGGAGACC GGCCTCGAGC TAATCGCCAT CTTCCAGCAG GCGCACCATT GCCCCTGTTT CACTATCCAG GTTACGGATA TAGTTCATGA CAATATTTAC ATTGGTCCAG CCACCAGCTT GCATGATCTC CGGTATTGAA ACTCCAGCGC GGGCCATATC TCGCGCGGCT CCGACACGGG CACTGTGTCC AGACCAGGCC AGGTATCTCT GACCAGAGTC ATCCTTAGCG CCGTAAATCA ATCGATGAGT TGCTTCAAAA ATCCCTTCCA GGGCGCGAGT TGATAGCTGG CTGGTGGCAG ATGGCGCGGC AACACCATTT TTTCTGACCC GGCAAAACAG GTAGTTATTC GGATCATCAG CTACACCAGA GACGGAAATC CATCGCTCGA CCAGTTTAGT TACCCCCAGG CTAAGTGCCT TCTCTACACC TGCGGTGCTA ACCAGCGTTT TCGTTCTGCC AATATGGATT AACATTCTCC CACCGTCAGT ACGTGAGATA TCTTTAACCC TGATCCTGGC AATTTCGGCT ATACGTAACA GGGTGTTATA AGCAATCCCC AGAAATGCCA GATTACGTAT ATCCTGGCAG CGATCGCTAT TTTCCATGAG TGAACGAACC TGGTCGAAAT CAGTGCGTTC GAACGCTAGA GCCTGTTTTG CACGTTCACC GGCATCAACG TTTTCTTTTC GGATCCGCCG CATAACCAGT GAAACAGCAT TGCTGTCACT TGGTCGTGGC AGCCCGGACC GACGATGAAG CATGTTTAGC TGGCCCAAAT GTTGCTGGAT AGTTTTTACT GCCAGACCGC GCGCCTGAAG ATATAGAAGA TAATCGCGAA CATCTTCAGG TTCTGCGGGA AACCATTTCC GGTTATTCAA CTTGCACCAT GCCGCCCACG ACCGGCAAAC GGACAGAAGC ATTTTCCAGG TATGCTCAGA AAACGCCTGG CGATCCCTGA ACATGTCCAT CAGGTTCTTG CGAACCTCAT CACTCGTTGC ATCGACCGGT AATGCAGGCA AATTTTGGTG TACGGTCAGT AAATTGGACA TCTGCAGCCC GGGACTAGTG GTACCGGATC CATGCAGAGT CTCCTGTTTT ACAACCGGGT GTACATAGCG AAATACTTGT AATGCGTGGT GATGCACCTG AATCTTTCTT CGAAACAGAT ACCAAATCCA AGCTAAAATC TTTTGTACTC ATTTTGAGTG CCTCCTTATA ATTTATTTTG TAGTTCCTTC GAACGAAATC ATTGTATCTA ACAAACTTCA GAATTTAATC AGAGCCGTTT ATTATGCTCG CGTTATCGAC AATAATATTA TTACCAATAC TTTCTCAAGA TAGAATTAAG ACTGTTTTAG ATTTGTTAAT GTTTCTATTG TCAGTATAGT TATAAGACT
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