pUC18T-mini-Tn7T-Gm-Pc-sfGFP
$99.00
In stock
SKU
PL35281
100μg liquid plasmid(Instantly):$120
| name | pUC18T-mini-Tn7T-Gm-Pc-sfGFP | another name | apUC18T-mini-Tn7T-Gm-Pc-sfGFP |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp,Gen | |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT AGAGGACCAG CCGCGTAACC TGGCAAAATC GGTTACGGTT GAGTAATAAA TGGATGCCCT GCGTAAGCGG GTGTGGGCGG ACAATAAAGT CTTAAACTGA ACAAAATAGA TCTAAACTAT GACAATAAAG TCTTAAACTA GACAGAATAG TTGTAAACTG AAATCAGTCC AGTTATGCTG TGAAAAAGCA TACTGGACTT TTGTTATGGC TAAAGCAAAC TCTTCATTTT CTGAAGTGCA AATTGCCCGT CGTATTAAAG AGGGGCGTGG GGTTCGAGGT CGACGGTATC GATAAGCTAG CTTAATTAGC TGAGCTTGGA CTCCTGTTGA TAGATCCAGT AATGACCTCA GAACTCCATC TGGATTTGTT CAGAACGCTC GGTTGCCGCC GGGCGTTTTT TATTGGTGAG AATCCAAGCT AGACTGCGAT GAGTGGCAGG GCGGGGCGTA ATTTTTTTAA GGCAGTTATT GGTGCCCTTA AACGCCTGGG GTAATGACTC TCTAGCTTGA GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGTTTT ATCTGTTGTT TGTCGGTGAA CGCTCTCCTG AGTAGGACAA ATCCGCCGCT AGGAGCTTGC GGCCCGGACG ATCGAATTGG GGATCTTGAA GTACCTATTC CGAAGTTCCT ATTCTCTAGA AAGTATAGGA ACTTCAGAGC GCTTTTGAAG CTGATGTGCT TAAAAACTTA CTCAATGGAA TTCTAGATCG AATTGGCCGC GGCGTTGTGA CAATTTACCG AACAACTCCG CGGCCGGGAA GCCGATCTCG GCTTGAACGA ATTGTTAGGT GGCGGTACTT GGGTCGATAT CAAAGTGCAT CACTTCTTCC CGTATGCCCA ACTTTGTATA GAGAGCCACT GCGGGATCGT CACCGTAATC TGCTTGCACG TAGATCACAT AAGCACCAAG CGCGTTGGCC TCATGCTTGA GGAGATTGAT GAGCGCGGTG GCAATGCCCT GCCTCCGGTG CTCGCCGGAG ACTGCGAGAT CATAGATATA GATCTCACTA CGCGGCTGCT CAAACTTGGG CAGAACGTAA GCCGCGAGAG CGCCAACAAC CGCTTCTTGG TCGAAGGCAG CAAGCGCGAT GAATGTCTTA CTACGGAGCA AGTTCCCGAG GTAATCGGAG TCCGGCTGAT GTTGGGAGTA GGTGGCTACG TCTCCGAACT CACGACCGAA AAGATCAAGA GCAGCCCGCA TGGATTTGAC TTGGTCAGGG CCGAGCCTAC ATGTGCGAAT GATGCCCATA CTTGAGCCAC CTAACTTTGT TTTAGGGCGA CTGCCCTGCT GCGTAACATC GTTGCTGCTG CGTAACATCG TTGCTGCTCC ATAACATCAA ACATCGACCC ACGGCGTAAC GCGCTTGCTG CTTGGATGCC CGAGGCATAG ACTGTACAAA AAAACAGTCA TAACAAGCCA TGAAAACCGC CACTGCGCCG TTACCACCGC TGCGTTCGGT CAAGGTTCTG GACCAGTTGC GTGAGCGCAT ACGCTACTTG CATTACAGTT TACGAACCGA ACAGGCTTAT GTCAATTCGA TCCATTGCTG TTGACAAAGG GAATCAGGGG ATCTTGAAGT TCCTATTCCG AAGTTCCTAT TCTCTAGAAA GTATAGGAAC TTCAGAGCGC TTTTGAAGCT AATTCGATCA TGCATGAGCT CTTATTACTT GTACAGTTCG TCCATGCCGT GGGTGATGCC GGCGGCGGTC ACGAATTCCA GCAGCACCAT GTGGTCGCGC TTTTCGTTCG GGTCCTTCGA CAGCACCGAC TGGGTCGACA GGTAGTGGTT GTCCGGCAGC AGCACCGGGC CGTCGCCGAT CGGGGTGTTC TGCTGGTAGT GGTCGGCCAG CTGCACCGAG CCGTCTTCCA CGTTGTGGCG GATCTTGAAG TTGGCCTTGA TGCCGTTCTT CTGCTTGTCG GCGGTGATGT ACACGTTGTG CGAGTTGAAG TTGTATTCCA GCTTGTGGCC CAGGATGTTG CCGTCTTCCT TGAAGTCGAT GCCCTTCAGT TCGATGCGGT TCACCAGGGT GTCGCCTTCG AACTTCACTT CGGCGCGGGT CTTGTAGGTG CCGTCGTCCT TGAACGAGAT GGTGCGTTCC TGCACGTAGC CTTCCGGCAT GGCCGACTTG AAGAAGTCGT GCTGCTTCAT GTGGTCCGGG TAGCGGGCGA AGCACTGCAC GCCGTAGGTC AGGGTGGTCA CCAGGGTCGG CCACGGCACC GGCAGCTTGC CGGTGGTGCA GATGAACTTC AGGGTCAGCT TGCCGTTGGT GGCGTCGCCT TCGCCTTCGC CGCGCACCGA GAACTTGTGG CCGTTCACGT CGCCGTCCAG TTCCACCAGG ATCGGCACCA CGCCGGTGAA CAGTTCTTCG CCCTTGCGCA TGTAGTCCTC CTGCCACCGT TTAATTCGGA TCCACGGGTT CGCTACCTGC ATTACAGCCT ACAGACCGAG AAGGCTTATG TCTACTGGGC CAAGGCATTT GTGTTGTGGA CGGCCCGCAG CCATGGTGGG TTTCGACATC CGCGCGAAAT GGGGCAAGCT GAAGTCGAGG GTTTTCTGAC CATGCTCGCC ACCGAGAAGC AAGTGGCGCC GGCCACCCAC CGGCAGGCGC TCAACGCGCT GTTGTTCTTG TATCGGCAGG TACCTCGCGA AGGCCTTGCA GGCCAACCAG ATAAGTGAAA TCTAGTTCCA AACTATTTTG TCATTTTTAA TTTTCGTATT AGCTTACGAC GCTACACCCA GTTCCCATCT ATTTTGTCAC TCTTCCCTAA ATAATCCTTA AAAACTCCAT TTCCACCCCT CCCAGTTCCC AACTATTTTG TCCGCCCACA GCGGGGCATT TTTCTTCCTG TTATGTTTGG GCGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CCCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GAGCTTATCG GCCAGCCTCG CAGAGCAGGA TTCCCGTTGA GCACCGCCAG GTGCGAATAA GGGACAGTGA AGAAGGAACA CCCGCTCGCG GGTGGGCCTA CTTCACCTAT CCTGCCCGGC TGACGCCGTT GGATACACCA AGGAAAGTCT ACACGAACCC TTTGGCAAAA TCCTGTATAT CGTGCGAAAA AGGATGGATA TACCGAAAAA ATCGCTATAA TGACCCCGAA GCAGGGTTAT GCAGCGGAAA GTATACCTTA AGGAATCCCC TGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCGAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTC
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