pBIFC-EEF2(human)-Myc-CC155
$84.00
In stock
SKU
PL34612
NM_001961.4;EEF-2; EF-2; EF2; SCA26;
100μg liquid plasmid(Instantly):$105
| name | pBIFC-EEF2(human)-Myc-CC155 | another name | apBIFC-EEF2(human)-Myc-CC155 |
| Insert Size (bp) | 2574bp | Plasmid host | Mammary cell |
| Plasmid uses | Bimolecular fluorescence | Fragment type | BIFC |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GAGTTCGAGC TTGCATGCCT GCAGGTCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGACAC CGGGACCGAT CCAGCCTCCG GACTCTAGAG GATCCGGTAC TAGAGGAACT GAAAAACCAG AAAGTTAACT GGTAAGTTTA GTCTTTTTGT CTTTTATTTC AGGTCCCGGA TCCGGTGGTG GTGCAAATCA AAGAACTGCT CCTCAGTGGA TGTTGCCTTT ACTTCTAGGC CTGTACGGAA GTGTTACTTC TGCTCTAAAA GCTGCGGAAT TGTACCCGCG GGCCCGCCAC CATGGTGAAC TTCACGGTAG ACCAGATCCG CGCCATCATG GACAAGAAGG CCAACATCCG CAACATGTCT GTCATCGCCC ACGTGGACCA TGGCAAGTCC ACGCTGACAG ACTCCCTGGT GTGCAAGGCG GGCATCATCG CCTCGGCCCG GGCCGGGGAG ACACGCTTCA CTGATACCCG GAAGGACGAG CAGGAGCGTT GCATCACCAT CAAGTCAACT GCCATCTCCC TCTTCTACGA GCTCTCGGAG AATGACTTGA ACTTCATCAA GCAGAGCAAG GACGGTGCCG GCTTCCTCAT CAACCTCATT GACTCCCCCG GGCATGTCGA CTTCTCCTCG GAGGTGACTG CTGCCCTCCG AGTCACCGAT GGCGCATTGG TGGTGGTGGA CTGCGTGTCA GGCGTGTGCG TGCAGACGGA GACAGTGCTG CGGCAGGCCA TTGCCGAGCG CATCAAGCCT GTGCTGATGA TGAACAAGAT GGACCGCGCC CTGCTGGAGC TGCAGCTGGA GCCCGAGGAG CTCTACCAGA CTTTCCAGCG CATCGTGGAG AACGTGAACG TCATCATCTC CACCTACGGC GAGGGCGAGA GCGGCCCCAT GGGCAACATC ATGATCGATC CTGTCCTCGG TACCGTGGGC TTTGGGTCTG GCCTCCACGG GTGGGCCTTC ACCCTGAAGC AGTTTGCCGA GATGTATGTG GCCAAGTTCG CCGCCAAGGG GGAGGGCCAG TTGGGGCCTG CCGAGCGGGC CAAGAAAGTA GAGGACATGA TGAAGAAGCT GTGGGGTGAC AGGTACTTTG ACCCAGCCAA CGGCAAGTTC AGCAAGTCAG CCACCAGCCC CGAAGGGAAG AAGCTGCCAC GCACCTTCTG CCAGCTGATC CTGGACCCCA TCTTCAAGGT GTTTGATGCG ATCATGAATT TCAAGAAAGA GGAGACAGCA AAACTGATAG AGAAACTGGA CATCAAACTG GACAGCGAGG ACAAGGACAA AGAAGGCAAA CCCCTGCTGA AGGCTGTGAT GCGCCGCTGG CTGCCTGCCG GAGACGCCTT GTTGCAGATG ATCACCATCC ACCTGCCCTC CCCTGTGACG GCCCAGAAGT ACCGCTGCGA GCTCCTGTAC GAGGGGCCCC CGGACGACGA GGCTGCCATG GGCATTAAAA GCTGTGACCC CAAAGGCCCT CTTATGATGT ATATTTCCAA AATGGTGCCA ACCTCCGACA AAGGTCGGTT CTACGCCTTT GGACGAGTCT TCTCGGGGCT GGTCTCCACT GGCCTGAAGG TCAGGATCAT GGGGCCCAAC TATACCCCTG GGAAGAAGGA GGACCTCTAC CTGAAGCCAA TCCAGAGAAC AATCTTGATG ATGGGCCGCT ACGTGGAGCC CATCGAGGAT GTGCCTTGTG GGAACATTGT GGGCCTCGTG GGCGTGGACC AGTTCCTGGT GAAGACGGGC ACCATCACCA CCTTCGAGCA CGCGCACAAC ATGCGGGTGA TGAAGTTCAG CGTCAGCCCT GTTGTCAGAG TGGCCGTGGA GGCCAAGAAC CCGGCTGACC TGCCCAAGCT GGTGGAGGGG CTGAAGCGGC TGGCCAAGTC CGACCCCATG GTGCAGTGCA TCATCGAGGA GTCGGGAGAG CATATCATCG CGGGCGCCGG CGAGCTGCAC CTGGAGATCT GCCTGAAGGA CCTGGAGGAG GACCACGCCT GCATCCCCAT CAAGAAATCT GACCCGGTCG TCTCGTACCG CGAGACGGTC AGTGAAGAGT CGAACGTGCT CTGCCTCTCC AAGTCCCCCA ACAAGCACAA CCGGCTGTAC ATGAAGGCGC GGCCCTTCCC CGACGGCCTG GCCGAGGACA TCGATAAAGG CGAGGTGTCC GCCCGTCAGG AGCTCAAGCA GCGGGCGCGC TACCTGGCCG AGAAGTACGA GTGGGACGTG GCTGAGGCCC GCAAGATCTG GTGCTTTGGG CCCGACGGCA CCGGCCCCAA CATCCTCACC GACATCACCA AGGGTGTGCA GTACCTCAAC GAGATCAAGG ACAGTGTGGT GGCCGGCTTC CAGTGGGCCA CCAAGGAGGG CGCACTGTGT GAGGAGAACA TGCGGGGTGT GCGCTTCGAC GTCCACGACG TCACCCTGCA CGCCGACGCC ATCCACCGCG GAGGGGGCCA GATCATCCCC ACAGCACGGC GCTGCCTCTA TGCCAGTGTG CTGACCGCCC AGCCACGCCT CATGGAGCCC ATCTACCTTG TGGAGATCCA GTGTCCAGAG CAGGTGGTCG GTGGCATCTA CGGGGTTTTG AACAGGAAGC GGGGCCACGT GTTCGAGGAG TCCCAGGTGG CCGGCACCCC CATGTTTGTG GTCAAGGCCT ATCTGCCCGT CAACGAGTCC TTTGGCTTCA CCGCTGACCT GAGGTCCAAC ACGGGCGGCC AGGCGTTCCC CCAGTGTGTG TTTGACCACT GGCAGATCCT GCCCGGAGAC CCCTTCGACA ACAGCAGCCG CCCCAGCCAG GTGGTGGCGG AGACCCGCAA GCGCAAGGGC CTGAAAGAAG GCATCCCTGC CCTGGACAAC TTCCTGGACA AATTGCTCGA GGAGCAGAAG CTGATCTCAG AGGAGGACCT GGGTACCCGT CCGGCGTGCA AAATCCCGAA CGACCTGAAA CAGAAAGTCA TGAACCACGA CAAGCAGAAG AACGGCATCA AGGCCAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG TAAGCGGCCG CGGGGATCCA GACATGATAA GATACATTGA TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT CGGATCCTCT AGAGTCGATC TGCAGGCATG CTAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGT
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